FastQCFastQC Report
Tue 31 May 2016
SRR212575_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212575_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91719
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT14741.607082501989773No Hit
CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT12941.4108309074455674No Hit
CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT11811.2876285175372604No Hit
CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC10571.15243297462903No Hit
CCAGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG9020.9834385459937417No Hit
CCAGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC7940.8656875892672184No Hit
CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6240.6803388610865797No Hit
CCAGACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC3390.3696071697249207No Hit
CCAGACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2860.31182197799801564No Hit
CCAGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA2680.2921968185435951No Hit
CCAGACGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT2600.28347452545274154No Hit
CCAGACGGGCTCCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTG2460.2682105125437477No Hit
CCAGACGGGATCCACTCCTATTATCCGTAGGTTTGGTCTTCTCATTGTGTCCTGGATT2380.2594882194528942No Hit
CCAGACGGGATAGTTACTTTTCTTTCTGTTTGGCTTATTCCAGCCCCGAGTTTGATTA2080.2267796203621932No Hit
CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.21260589408955613No Hit
CCAGACGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATG1920.20933503418048605No Hit
CCAGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1850.20170302772598916No Hit
CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1770.19298073463513557No Hit
CCAGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCAGAAAAACATCGAACT1770.19298073463513557No Hit
CCAGACGGGACACAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACCACTTCTGCCC1680.1831681549079253No Hit
CCAGACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1570.1711750019080016No Hit
CCAGACGGGGCTCCAGACGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTA1520.16572356872621813No Hit
CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT1500.16354299545350473No Hit
CCAGACGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA1460.15918184890807793No Hit
CCAGACGGGGCTCCAGACGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGA1420.15482070236265114No Hit
CCAGACGGGGGGAATTCTTTCGGCAACATTAACTGGTCGATGCTGAAAAACATCGAAC1390.15154984245358105No Hit
CCAGACGGGGTTGCTGGGCTTGCGTGAGCCACCCCGTGTAGTGTTGGGCTTGTATGAG1340.14609840927179754No Hit
CCAGACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1330.14500812263544086No Hit
CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1270.1384664028173007No Hit
CCAGACGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA1260.13737611618094397No Hit
CCAGACGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1250.13628582954458726No Hit
CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1190.1297441097264471No Hit
CCAGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1180.12865382309009038No Hit
CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1130.1232023899083069No Hit
CCAGACGGGGAGTGCCCCAGACTTTAACTGTGTAAATAAGAGTGTTTCATTCAAATGT1130.1232023899083069No Hit
CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC1110.1210218166355935No Hit
CCAGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.11775095672652341No Hit
CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1050.1144800968174533No Hit
CCAGACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1040.1133898101810966No Hit
CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1030.1122995235447399No Hit
CCAGACGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG1020.11120923690838322No Hit
CCAGACGGGAAGATTGCCGTGGCCCTGGCTGTTGCCGCAATCCCTGAGGGTCTGCCTG980.10684809036295644No Hit
CCAGACGGGATTAAAGGCATGCACTACAATATATGACTTTCAACTTGACTTATTTCCA970.10575780372659971No Hit
CCAGACGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTG940.10248694381752962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCA251.5941188E-552.2664836
CGGGTAT503.6379788E-1252.2664836
CGGGTAC353.67927E-852.2664836
GGATGTA353.67927E-852.2664838
GAATGGT150.007054858452.2664839
ATGGCAA150.007054858452.26648310
GGATAGT307.639155E-752.2664838
AGGCATT150.007054858452.26648310
GATGGCA150.007054858452.2664839
CGGGCAC203.345318E-452.2664836
GGAGTTT203.345318E-452.2664838
ATCCACT203.345318E-452.26648310
GGAGTAC150.007054858452.2664838
GATGCCA503.6379788E-1252.2664839
GAGTTTC203.345318E-452.2664839
GGATTGA150.007054858452.2664838
GGATTAC650.052.2664838
GAACAGT307.639155E-752.2664839
GATTACC650.052.2664839
GGGGGTA150.007054858452.2664837
Produced by FastQC (version 0.11.5)