FastQCFastQC Report
Tue 31 May 2016
SRR212574_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212574_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162910
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT36382.233134859738506No Hit
CCAGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA22441.3774476704929102No Hit
CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC20691.2700263949419925No Hit
CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC18371.1276164753544902No Hit
CCAGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA12320.7562457798784605No Hit
CCAGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA12130.7445828985329324No Hit
CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC10360.6359339512614327No Hit
CCAGACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC7410.45485237247560006No Hit
CCAGACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG6160.37812288993923027No Hit
CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5660.3474310969246823No Hit
CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.31244245288809774No Hit
CCAGACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4660.2860475108955865No Hit
CCAGACGGGACACAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACCACTTCTGC4350.26701859922656684No Hit
CCAGACGGGATAGTTACTTTTCTTTCTGTTTGGCTTATTCCAGCCCCGAGTTTGAT4010.24614817997667426No Hit
CCAGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG3670.22527776072678163No Hit
CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC3590.220367073844454No Hit
CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC3590.220367073844454No Hit
CCAGACGGGATCTTTAATCTAGAATGATCATTATGACTGACAAACACACATATATT3100.19028911669019705No Hit
CCAGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.18967528082990606No Hit
CCAGACGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3080.18906144496961513No Hit
CCAGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.17985390706525076No Hit
CCAGACGGGATCCACTCCTATTATCCGTAGGTTTGGTCTTCTCATTGTGTCCTGGA2890.17739856362408693No Hit
CCAGACGGGCTCCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCG2870.176170891903505No Hit
CCAGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC2730.1675771898594316No Hit
CCAGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2700.1657356822785587No Hit
CCAGACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2610.1602111595359401No Hit
CCAGACGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT2610.1602111595359401No Hit
CCAGACGGGCAGTTTCTCAATCATCTCAAATACCCACAGGCAAGTTAACTGGTGAG2580.15836965195506722No Hit
CCAGACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2510.1540728009330305No Hit
CCAGACGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA2450.15038978577128476No Hit
CCAGACGGGGCTCCAGACGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCG2430.14916211405070284No Hit
CCAGACGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC2400.14732060646982995No Hit
CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2350.14425142716837516No Hit
CCAGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2260.13872690442575655No Hit
CCAGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.13258854582284696No Hit
CCAGACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG2130.1307470382419741No Hit
CCAGACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2080.1276778589405193No Hit
CCAGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCAGAAAAACATCGAA2030.12460867963906451No Hit
CCAGACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2000.12276717205819164No Hit
CCAGACGGGATGTCTTTAAGAAATGTTTGCAAACTGTCATTTCCAACCTTACAGAT1810.11110429071266342No Hit
CCAGACGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC1800.11049045485237248No Hit
CCAGACGGGGGGAATTCTTTCGGCAACATTAACTGGTCGATGCTGAAAAACATCGA1760.10803511141120864No Hit
CCAGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1720.10557976797004481No Hit
CCAGACGGGGTTGCTGGGCTTGCGTGAGCCACCCCGTGTAGTGTTGGGCTTGTATG1710.10496593210975384No Hit
CCAGACGGGAAGGACTGCACGGTGAAGATCTGGAACAACGACCTGACCATCTCACT1680.10312442452888099No Hit
CCAGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC1640.10066908108771713No Hit
CCAGACGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC1630.10005524522742619No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGTC204.066727E-450.2624648
TTGTATG204.066727E-450.2624650
CACAACC204.066727E-450.2624647
CATCAGT204.066727E-450.2624647
TCGCCAC301.0052863E-650.26245548
ATCTCAC150.00824557550.26245549
GCGACTG150.00824557550.26245550
CATATAT301.0052863E-650.26245549
TACCATC150.00824557550.26245547
ATGTGGC150.00824557550.26245549
TGGGTCG150.00824557550.26245547
CCCGTCA150.00824557550.26245550
TATCTCA252.0160354E-550.26245548
AATTGTC150.00824557550.26245548
CCTCATG150.00824557550.26245549
ATCCCTA150.00824557550.26245548
GAAAACC150.00824557550.26245550
ACATATA301.0052863E-650.26245548
ATCGAAC252.0160354E-550.26245550
TAATATG204.0791702E-450.23136543
Produced by FastQC (version 0.11.5)