##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212574_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 162910 Sequences flagged as poor quality 0 Sequence length 56 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.01642010926278 39.0 38.0 40.0 35.0 40.0 2 37.897501688048614 39.0 38.0 40.0 35.0 40.0 3 37.36978699895648 39.0 37.0 40.0 33.0 40.0 4 37.183107237124794 39.0 36.0 39.0 33.0 40.0 5 36.940451783193176 39.0 36.0 39.0 33.0 40.0 6 37.61608863789822 39.0 38.0 40.0 34.0 40.0 7 37.89095205941931 39.0 38.0 40.0 35.0 40.0 8 37.813848137008165 39.0 38.0 40.0 35.0 40.0 9 37.732686759560494 39.0 38.0 40.0 35.0 40.0 10 36.355325026088025 38.0 35.0 39.0 31.0 40.0 11 36.47793260082254 38.0 36.0 39.0 31.0 40.0 12 36.38955251365785 38.0 35.0 39.0 31.0 40.0 13 36.31757412068013 38.0 35.0 39.0 31.0 40.0 14 36.247799398440854 38.0 35.0 39.0 30.0 40.0 15 36.26862071082193 38.0 35.0 39.0 30.0 40.0 16 36.02481738383156 38.0 35.0 39.0 30.0 40.0 17 35.88833098029587 38.0 35.0 39.0 30.0 40.0 18 35.792548032656065 38.0 35.0 39.0 29.0 40.0 19 35.44774415321343 38.0 35.0 39.0 29.0 40.0 20 35.54550365232337 38.0 35.0 39.0 29.0 40.0 21 35.26993431956295 38.0 35.0 39.0 29.0 40.0 22 35.00942851881407 38.0 34.0 39.0 28.0 40.0 23 34.661045976305935 38.0 33.0 39.0 27.0 40.0 24 34.43612424037812 38.0 33.0 39.0 27.0 40.0 25 34.347326744828436 38.0 33.0 39.0 27.0 40.0 26 33.66516481492849 37.0 33.0 39.0 23.0 40.0 27 33.433681173654165 37.0 33.0 39.0 23.0 40.0 28 32.965821619299 36.0 32.0 39.0 22.0 40.0 29 32.95689644589037 36.0 32.0 39.0 22.0 40.0 30 32.53996071450494 36.0 32.0 39.0 18.0 40.0 31 31.643349088453746 36.0 30.0 39.0 10.0 40.0 32 31.33740101896753 36.0 30.0 39.0 8.0 40.0 33 31.092204284574304 35.0 30.0 38.0 7.0 40.0 34 31.08886501749432 36.0 30.0 39.0 2.0 40.0 35 30.693517893315327 35.0 29.0 39.0 2.0 40.0 36 30.682125099748326 35.0 30.0 39.0 2.0 40.0 37 30.376674237308944 35.0 29.0 39.0 2.0 40.0 38 30.250365232336872 35.0 29.0 39.0 2.0 40.0 39 30.090688109999387 35.0 29.0 39.0 2.0 40.0 40 29.79677122337487 35.0 28.0 39.0 2.0 40.0 41 29.475458842305567 35.0 28.0 39.0 2.0 40.0 42 29.127751519243755 35.0 27.0 39.0 2.0 40.0 43 28.838174452151495 35.0 26.0 39.0 2.0 40.0 44 28.795568105088698 35.0 26.0 39.0 2.0 40.0 45 28.284592719906698 35.0 25.0 39.0 2.0 40.0 46 27.42598981032472 34.0 21.0 39.0 2.0 40.0 47 27.268516358725677 34.0 20.0 38.0 2.0 39.0 48 27.24391995580382 34.0 21.0 38.0 2.0 39.0 49 26.862463937143207 34.0 18.0 38.0 2.0 39.0 50 26.486397397335953 34.0 13.0 38.0 2.0 39.0 51 25.847842366951078 33.0 2.0 38.0 2.0 39.0 52 25.52687987232214 33.0 2.0 38.0 2.0 39.0 53 25.224989257872444 33.0 2.0 38.0 2.0 39.0 54 24.868706647842366 33.0 2.0 38.0 2.0 39.0 55 24.27516420109263 33.0 2.0 38.0 2.0 39.0 56 13.880553679945983 18.0 2.0 22.0 2.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.2223455727274342 1 2 -1.4607311119172124 1 3 -2.142316121155268 1 4 -2.2540532885651317 1 5 -2.0937002324238705 1 6 -2.04506532756907 1 7 -1.517880631979402 1 8 -1.5559379960582618 1 9 -1.950355211024828 1 10 -2.739062646663143 1 11 -2.9009682050114662 1 12 -2.427474453860519 1 13 -2.3372983899573825 1 14 -2.338209928694674 1 15 -2.4322724761047922 1 16 -3.109003337388195 1 17 -2.8223129045257735 1 18 -2.5624502467919754 1 19 -3.5794521662832395 1 20 -3.111828147654961 1 21 -2.9979578191390317 1 22 -2.898913641921453 1 23 -3.3659485247028016 1 24 -2.854116765617512 1 25 -3.6301746266940356 1 26 -4.222384492617991 1 27 -3.8813048625933284 1 28 -4.094764857786451 1 29 -3.6518412786641115 1 30 -3.7985528782042977 1 31 -2.7811681925701954 1 32 -2.156047190956187 1 33 -1.637544461670398 1 34 -1.9219226646168792 1 35 -2.7063265259799216 1 36 -1.092314700340122 1 37 -1.6575509348590778 1 38 -1.6922237977915309 1 39 -0.9620913748044906 1 40 -0.9827726946203725 1 41 -1.6782358086266846 1 42 -1.1291395479737467 1 43 -1.366942117998331 1 44 -2.223007996580229 1 45 -1.957771867598133 1 46 -1.611652625237177 1 47 -2.033364956608576 1 48 -1.4347865578260475 1 49 -1.9418169234387257 1 50 -1.2977705643289745 1 51 -1.9828789829449676 1 52 -2.177560773543547 1 53 -2.9623764587444334 1 54 -2.5964675573528737 1 55 -3.7686542917988817 1 56 -2.6709202280037587 2 1 -0.016965710859025762 2 2 -0.3180557211502162 2 3 -0.587790494110557 2 4 -0.5936873610236262 2 5 -0.6361090534586467 2 6 -0.26631820531049755 2 7 -0.18163926520368534 2 8 -0.10919135847193218 2 9 0.05715720870133367 2 10 0.001213616513879856 2 11 -0.743267974792154 2 12 -0.6116499052115429 2 13 -0.7654457303184614 2 14 0.039713250755227136 2 15 -0.8708402696344137 2 16 -1.329529206769024 2 17 -0.4391189144615595 2 18 -0.8045440308704954 2 19 -0.6778406310839173 2 20 -0.4747870716825915 2 21 -0.33959116458917293 2 22 0.27387201178022735 2 23 0.22341291962047194 2 24 6.633143534102715E-4 2 25 0.059453387361458 2 26 0.04182246242865517 2 27 -0.6787724501372523 2 28 -1.4591172145052198 2 29 -0.4194409393935459 2 30 -2.072877366269246 2 31 -0.9326888517244427 2 32 -0.8375569449850282 2 33 -0.9928697979117658 2 34 -1.260526808564535 2 35 -0.3738413690672857 2 36 0.055328580890947165 2 37 0.17990211251763455 2 38 -0.47466654588313517 2 39 -0.0713849650141114 2 40 -0.005128201708703983 2 41 -0.4712807619769812 2 42 -0.3795333441690687 2 43 -0.5359715618369343 2 44 0.03762450069348944 2 45 0.3534482947670661 2 46 -0.3488391028816693 2 47 -0.5114941217606415 2 48 -1.4623522914986502 2 49 -0.4246110589046168 2 50 -1.4711620583311564 2 51 -1.653786531715113 2 52 -1.1942819658372592 2 53 -1.1754504924291567 2 54 -1.2220582516458585 2 55 -2.2897375342561688 2 56 -1.6015515135968759 3 1 -0.45008884394896853 3 2 -0.3023460187495104 3 3 -0.518092518670791 3 4 0.033041259398984835 3 5 -0.37872438701324285 3 6 -0.11580376787272684 3 7 -0.09241479347008408 3 8 -0.07871232318041166 3 9 -0.017297930141459972 3 10 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220.0 7 641.0 8 329.0 9 477.0 10 599.0 11 602.0 12 776.0 13 1141.0 14 1168.0 15 1251.0 16 1511.0 17 1747.0 18 1750.0 19 2057.0 20 2443.0 21 2245.0 22 2008.0 23 2442.0 24 2729.0 25 2926.0 26 2830.0 27 3307.0 28 3843.0 29 4420.0 30 5182.0 31 6157.0 32 7164.0 33 8922.0 34 11259.0 35 13820.0 36 16905.0 37 20933.0 38 19366.0 39 9235.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 41.4191885089927 36.710453624700754 6.204039039960715 15.666318826345835 11 25.852924927874287 28.07746608556872 18.982260143637593 27.0873488429194 12 35.437358050457306 14.827205205328095 28.71340003683015 21.022036707384444 13 18.049843471855624 16.627585783561475 32.07353753606286 33.24903320852004 14 16.281996194217665 27.585169725615373 35.28205757780369 20.85077650236327 15 28.28310109876619 17.41636486403536 35.12061874654717 19.17991529065128 16 22.310478178135167 17.313854275366765 33.88558099564177 26.4900865508563 17 30.215456386962124 18.57712847584556 18.543367503529556 32.664047633662754 18 31.01282916948008 16.049352403167394 24.682953778159717 28.254864649192807 19 26.31023264379105 17.98170769136333 33.039715180160826 22.668344484684795 20 27.32060646982997 25.347738014854826 24.84868946043828 22.482966054876925 21 29.859431587993367 15.813025596955374 32.54987416364864 21.777668651402614 22 32.284083236142656 16.826468602295748 21.73715548462341 29.152292676938185 23 30.599717635504263 27.4544226873734 22.586090479405808 19.35976919771653 24 26.118102019519977 31.43453440549997 22.80645755325026 19.64090602172979 25 25.45473526485796 23.53692536323553 22.48866999914027 28.519669372766238 26 32.02012198492694 18.209290755311503 23.902534903290398 25.86805235647116 27 21.18696149166042 19.822637938027754 25.493799087992723 33.49660148231911 28 23.8329419575347 27.722930523623933 27.70571818483593 20.738409334005432 29 30.770507830514106 16.534159733879346 30.51963623618823 22.175696199418322 30 19.843242445122552 25.48294615611773 31.143567280244117 23.530244118515604 31 29.637719503341003 19.3845907731686 30.92059116695175 20.057098556538648 32 28.68320129026698 15.842705620910206 36.6765775914606 18.797515497362216 33 30.510927329716548 14.721874980756322 33.67920635995837 21.087991329568755 34 27.099499913778235 16.691301455916044 37.05749759810805 19.151701032197668 35 23.570720168538287 26.31131535016663 36.59239729698097 13.525567184314111 36 26.224547650742004 23.19614948479022 33.66735689986689 16.911945964600896 37 22.22235920702239 21.477093401716147 39.460868921984414 16.83967846927705 38 24.389100931686595 20.120360835866542 36.86297239469968 18.62756583774718 39 23.102204408817638 20.69122861106829 36.95822414058887 19.248342839525204 40 24.077432727541918 19.964590196296157 39.38322784419687 16.574749231965058 41 25.097625525142043 17.774323098847 38.874391575518665 18.25365980049229 42 21.7578129823305 18.7801745959228 38.19501895365923 21.26699346808747 43 22.49666633741604 18.64505136309759 39.495876135914656 19.36240616357171 44 25.188766028906052 15.663721731399677 38.737320417600465 20.410191822093807 45 22.902498532999786 20.47808764940239 38.40143302757961 18.21798079001822 46 21.205512963866735 20.359788229043758 39.74720923192873 18.68748957516077 47 23.01307722733116 19.707307426085237 39.00425195293187 18.275363393651737 48 24.749579540957658 19.66264345073209 36.95463988919668 18.633137119113574 49 21.568445705034325 20.975226937075554 36.84630072483918 20.610026633050936 50 17.62705787075675 19.315392842349947 39.82516985354575 23.232379433347553 51 17.437913830517278 21.697547464930665 41.41860019412554 19.44593851042652 52 19.04237686465626 19.757934839076764 44.740617965019915 16.459070331247062 53 15.791068777832756 21.79428500742207 41.48812469074715 20.92652152399802 54 18.76543057093886 20.869866531362348 39.88837393494254 20.47632896275625 55 19.363040775041295 22.213148728989193 37.75171210630835 20.672098389661166 56 17.972210536735048 22.40046027071837 35.5651926455668 24.062136546979783 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 32.5 13 123.0 14 181.0 15 249.0 16 317.0 17 398.5 18 480.0 19 890.0 20 1300.0 21 1670.5 22 2109.0 23 2177.0 24 2348.0 25 2519.0 26 3192.0 27 3865.0 28 4467.0 29 5534.5 30 6000.0 31 5808.0 32 5616.0 33 5463.5 34 5311.0 35 4559.0 36 3807.0 37 5211.5 38 4819.0 39 3022.0 40 2596.0 41 2170.0 42 2186.0 43 2202.0 44 2306.5 45 2411.0 46 4058.0 47 5237.5 48 4770.0 49 4218.0 50 3666.0 51 3487.0 52 3308.0 53 3941.0 54 5653.0 55 6732.0 56 7011.0 57 7290.0 58 7083.0 59 6876.0 60 8455.5 61 10035.0 62 9842.0 63 9681.0 64 9713.0 65 11170.0 66 12627.0 67 10143.0 68 7659.0 69 6560.0 70 5461.0 71 4452.5 72 2816.5 73 2189.0 74 1874.0 75 1559.0 76 1309.0 77 1059.0 78 840.0 79 506.0 80 391.0 81 291.0 82 191.0 83 145.5 84 100.0 85 78.0 86 56.0 87 46.0 88 23.5 89 11.0 90 11.0 91 11.0 92 8.5 93 6.0 94 4.0 95 2.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.04174083849978515 26 0.0632250936099687 27 0.11785648517586397 28 0.14486526302866615 29 0.17003253330059542 30 0.22466392486649073 31 0.23571297035172795 32 0.28420600331471363 33 0.3185808114910073 34 0.33024369283653554 35 0.3517279479467191 36 0.39653796574795896 37 0.4210914001595973 38 0.44871401387269044 39 0.45116935731385427 40 0.4959793751150942 41 0.49782088269596714 42 0.5745503652323369 43 0.5684120066294273 44 0.5751642010926279 45 0.6230433981953226 46 0.6365477871217237 47 0.676447118040636 48 0.7255539868639126 49 0.6635565649745258 50 0.7077527469154749 51 0.7114357620772205 52 0.747652077834387 53 0.7550181081578786 54 0.7973727825179547 55 0.777730034988644 56 0.7771161991283531 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 162910.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.013074703824195 #Duplication Level Percentage of deduplicated Percentage of total 1 73.89631496898737 27.351298262844516 2 10.45308302099572 7.73801485482782 3 4.6120932700918775 5.1212325824074645 4 2.5058874257852666 3.7100239395985515 5 1.7413512885999536 3.2226382665275306 6 1.2438223489999667 2.762261371309312 7 0.887259942286643 2.2988152967896385 8 0.6567382002719825 1.9446320054017558 9 0.5771335699359846 1.922533914431281 >10 3.083020995721251 20.561659812166226 >50 0.20398686523599457 5.158062734024922 >100 0.12106537530266344 8.57099011724265 >500 0.00663371919466649 1.4928488122276102 >1k 0.011609008590666357 8.144988030200723 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 3638 2.233134859738506 No Hit CCAGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 2244 1.3774476704929102 No Hit CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 2069 1.2700263949419925 No Hit CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 1837 1.1276164753544902 No Hit CCAGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 1232 0.7562457798784605 No Hit CCAGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 1213 0.7445828985329324 No Hit CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 1036 0.6359339512614327 No Hit CCAGACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC 741 0.45485237247560006 No Hit CCAGACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 616 0.37812288993923027 No Hit CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 566 0.3474310969246823 No Hit CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 509 0.31244245288809774 No Hit CCAGACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 466 0.2860475108955865 No Hit CCAGACGGGACACAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACCACTTCTGC 435 0.26701859922656684 No Hit CCAGACGGGATAGTTACTTTTCTTTCTGTTTGGCTTATTCCAGCCCCGAGTTTGAT 401 0.24614817997667426 No Hit CCAGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 367 0.22527776072678163 No Hit CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 359 0.220367073844454 No Hit CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC 359 0.220367073844454 No Hit CCAGACGGGATCTTTAATCTAGAATGATCATTATGACTGACAAACACACATATATT 310 0.19028911669019705 No Hit CCAGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 309 0.18967528082990606 No Hit CCAGACGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 308 0.18906144496961513 No Hit CCAGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 293 0.17985390706525076 No Hit CCAGACGGGATCCACTCCTATTATCCGTAGGTTTGGTCTTCTCATTGTGTCCTGGA 289 0.17739856362408693 No Hit CCAGACGGGCTCCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCG 287 0.176170891903505 No Hit CCAGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 273 0.1675771898594316 No Hit CCAGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 270 0.1657356822785587 No Hit CCAGACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 261 0.1602111595359401 No Hit CCAGACGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT 261 0.1602111595359401 No Hit CCAGACGGGCAGTTTCTCAATCATCTCAAATACCCACAGGCAAGTTAACTGGTGAG 258 0.15836965195506722 No Hit CCAGACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 251 0.1540728009330305 No Hit CCAGACGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA 245 0.15038978577128476 No Hit CCAGACGGGGCTCCAGACGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCG 243 0.14916211405070284 No Hit CCAGACGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC 240 0.14732060646982995 No Hit CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 235 0.14425142716837516 No Hit CCAGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 226 0.13872690442575655 No Hit CCAGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 216 0.13258854582284696 No Hit CCAGACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG 213 0.1307470382419741 No Hit CCAGACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 208 0.1276778589405193 No Hit CCAGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCAGAAAAACATCGAA 203 0.12460867963906451 No Hit CCAGACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC 200 0.12276717205819164 No Hit CCAGACGGGATGTCTTTAAGAAATGTTTGCAAACTGTCATTTCCAACCTTACAGAT 181 0.11110429071266342 No Hit CCAGACGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC 180 0.11049045485237248 No Hit CCAGACGGGGGGAATTCTTTCGGCAACATTAACTGGTCGATGCTGAAAAACATCGA 176 0.10803511141120864 No Hit CCAGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 172 0.10557976797004481 No Hit CCAGACGGGGTTGCTGGGCTTGCGTGAGCCACCCCGTGTAGTGTTGGGCTTGTATG 171 0.10496593210975384 No Hit CCAGACGGGAAGGACTGCACGGTGAAGATCTGGAACAACGACCTGACCATCTCACT 168 0.10312442452888099 No Hit CCAGACGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC 164 0.10066908108771713 No Hit CCAGACGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 163 0.10005524522742619 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.006138358602909582 0.0 0.0 0.0 0.0 11 0.009821373764655332 0.0 0.0 0.0 0.0 12 0.009821373764655332 0.0 0.0 0.0 0.0 13 0.01043520962494629 0.0 0.0 0.0 0.0 14 0.01718740408814683 0.0 0.0 0.0 0.0 15 0.018415075808728747 0.0 0.0 0.0 0.0 16 0.018415075808728747 0.0 0.0 0.0 0.0 17 0.018415075808728747 0.0 0.0 0.0 0.0 18 0.018415075808728747 0.0 0.0 0.0 0.0 19 0.019028911669019703 0.0 0.0 0.0 0.0 20 0.02700877785280216 0.0 0.0 0.0 0.0 21 0.028850285433675037 0.0 0.0 0.0 0.0 22 0.03253330059542078 0.0 0.0 0.0 0.0 23 0.03683015161745749 0.0 0.0 0.0 0.0 24 0.04603768952182186 0.0 0.0 0.0 0.0 25 0.04603768952182186 0.0 0.0 0.0 0.0 26 0.04665152538211282 0.0 0.0 0.0 0.0 27 0.04665152538211282 0.0 0.0 0.0 0.0 28 0.10005524522742619 0.0 0.0 0.0 0.0 29 0.10066908108771715 0.0 0.0 0.0 0.0 30 0.13258854582284696 0.0 0.0 0.0 0.0 31 0.13443005340371986 0.0 0.0 0.0 0.0 32 0.1393407402860475 0.0 0.0 0.0 0.0 33 0.1454790988889571 0.0 0.0 0.0 0.0 34 0.14916211405070284 0.0 0.0 0.0 0.0 35 0.2553557178810386 0.0 0.0 0.0 0.0 36 0.25903873304278435 0.0 0.0 0.0 0.0 37 0.26456325578540296 0.0 0.0 0.0 0.0 38 0.26640476336627583 0.0 0.0 0.0 0.0 39 0.2707016143883126 0.0 0.0 0.0 0.0 40 0.2749984654103493 0.0 0.0 0.0 0.0 41 0.2805229881529679 0.0 0.0 0.0 0.0 42 0.2829783315941317 0.0 0.0 0.0 0.0 43 0.2848198391750046 0.0 0.0 0.0 0.0 44 0.2885028543367504 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCAGTC 20 4.066727E-4 50.26246 48 TTGTATG 20 4.066727E-4 50.26246 50 CACAACC 20 4.066727E-4 50.26246 47 CATCAGT 20 4.066727E-4 50.26246 47 TCGCCAC 30 1.0052863E-6 50.262455 48 ATCTCAC 15 0.008245575 50.262455 49 GCGACTG 15 0.008245575 50.262455 50 CATATAT 30 1.0052863E-6 50.262455 49 TACCATC 15 0.008245575 50.262455 47 ATGTGGC 15 0.008245575 50.262455 49 TGGGTCG 15 0.008245575 50.262455 47 CCCGTCA 15 0.008245575 50.262455 50 TATCTCA 25 2.0160354E-5 50.262455 48 AATTGTC 15 0.008245575 50.262455 48 CCTCATG 15 0.008245575 50.262455 49 ATCCCTA 15 0.008245575 50.262455 48 GAAAACC 15 0.008245575 50.262455 50 ACATATA 30 1.0052863E-6 50.262455 48 ATCGAAC 25 2.0160354E-5 50.262455 50 TAATATG 20 4.0791702E-4 50.231365 43 >>END_MODULE