FastQCFastQC Report
Tue 31 May 2016
SRR212573_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212573_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150444
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT30862.0512615990002923No Hit
CCAGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA21361.4197973996968971No Hit
CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC20091.3353806067373906No Hit
CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC18201.209752466033873No Hit
CCAGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA12360.8215681582515754No Hit
CCAGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA11730.7796921113504028No Hit
CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC10610.7052458057483183No Hit
CCAGACGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC9690.6440934832894631No Hit
CCAGACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG5790.38486081199649036No Hit
CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4950.329026082794927No Hit
CCAGACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT4620.30709101060859856No Hit
CCAGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4530.3011087181941453No Hit
CCAGACGGGACACAGCGGCCTGGAGTCGGAAGCAGAACGCACAGGACCACTTCTGC4460.29645582409401505No Hit
CCAGACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG3830.2545797771928425No Hit
CCAGACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC3550.2359682007923214No Hit
CCAGACGGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3390.2253330142777379No Hit
CCAGACGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT3180.21137433197734706No Hit
CCAGACGGGATAGTTACTTTTCTTTCTGTTTGGCTTATTCCAGCCCCGAGTTTGAT2970.1974156496769562No Hit
CCAGACGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2880.191433357262503No Hit
CCAGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.19076865810534152No Hit
CCAGACGGGCTCCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCG2830.18810986147669564No Hit
CCAGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.18611576400521124No Hit
CCAGACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC2800.18611576400521124No Hit
CCAGACGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC2650.17614527664778923No Hit
CCAGACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2530.1681688867618516No Hit
CCAGACGGGCAGTTTCTCAATCATCTCAAATACCCACAGGCAAGTTAACTGGTGAG2490.1655100901332057No Hit
CCAGACGGGGCTCCAGACGGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCG2460.16351599266172132No Hit
CCAGACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG2360.15686900109010662No Hit
CCAGACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2300.1528808061471378No Hit
CCAGACGGGATCTTTAATCTAGAATGATCATTATGACTGACAAACACACATATATT2280.15155140783281484No Hit
CCAGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC2270.1508867086756534No Hit
CCAGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.148892611204169No Hit
CCAGACGGGATCCACTCCTATTATCCGTAGGTTTGGTCTTCTCATTGTGTCCTGGA2200.14623381457552312No Hit
CCAGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2190.14556911541836165No Hit
CCAGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2180.14490441626120018No Hit
CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2110.1402515221610699No Hit
CCAGACGGGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA2100.13958682300390843No Hit
CCAGACGGGATGTCTTTAAGAAATGTTTGCAAACTGTCATTTCCAACCTTACAGAT1840.12230464491771025No Hit
CCAGACGGGAAGGACTGCACGGTGAAGATCTGGAACAACGACCTGACCATCTCACT1830.12163994576054878No Hit
CCAGACGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC1820.12097524660338731No Hit
CCAGACGGGGGGAATTCTTTCGGCAACATTAACTGGTCGATGCTGAAAAACATCGA1810.12031054744622584No Hit
CCAGACGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC1780.11831644997474143No Hit
CCAGACGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCAGAAAAACATCGAA1770.11765175081757996No Hit
CCAGACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1740.11565765334609557No Hit
CCAGACGGGGTTGCTGGGCTTGCGTGAGCCACCCCGTGTAGTGTTGGGCTTGTATG1710.11366355587461115No Hit
CCAGACGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA1620.10768126346015792No Hit
CCAGACGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC1580.10502246683151206No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACGT150.00820718150.3199150
AGTTATA150.00820718150.3199150
CATCCAA204.042846E-450.3199150
ATCGAAC150.00820718150.3199150
TAATCGC150.00820718150.3199150
CTTGCTC252.0016985E-550.31990450
TTTTACG252.0016985E-550.31990450
ATGGACG150.00821808150.3030249
TTGTCTG150.00821808150.3030249
AGCCACG150.00821808150.3030249
AAATTCT150.00821808150.3030249
CTAATCG150.00821808150.3030249
GCTTGCT252.0056797E-550.30301749
ATCAGTC204.0562646E-450.28614848
GTAATGG204.0562646E-450.28614846
TTCTAAT150.00822899150.28614447
TAGTTCC150.00822899150.28614446
TCTAATC150.00822899150.28614448
ACATATA355.0171366E-850.28614448
CACTTCT900.050.28614448
Produced by FastQC (version 0.11.5)