FastQCFastQC Report
Tue 31 May 2016
SRR212572_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212572_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36638
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT10502.865876958349255No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA8212.2408428407664176No Hit
TCGTAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC6011.6403733828265734No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5091.3892679731426387No Hit
TCGTAAGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3570.9743981658387466No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3180.8679513073857743No Hit
TCGTAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG2830.7724220754407991No Hit
TCGTAAGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTG2220.6059282711938425No Hit
TCGTAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2010.5486107320268574No Hit
TCGTAAGGGGGGTTAGAGGGGGAAGGGGTTGTGTGTTTTCTTTTTTTGAGACAAGGCT1770.4831049729788744No Hit
TCGTAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1690.46126971996288No Hit
TCGTAAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC1540.42032862055789066No Hit
TCGTAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1500.4094109940498936No Hit
TCGTAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1450.3957639609148971No Hit
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1360.3711993012719035No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1200.32752879523991485No Hit
TCGTAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1170.31934057535891697No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG1130.3084229488509198No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1110.30296413559692126No Hit
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT1070.29204650908892404No Hit
TCGTAAGGGGTGGTATCAACGCAGAATACAGTGCTTCGTAAGGGGGGGCTGGTGAGAT1060.2893171024619248No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1060.2893171024619248No Hit
TCGTAAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC1000.27294066269992906No Hit
TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.2702112560729298No Hit
TCGTAAGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC980.26748184944593045No Hit
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT880.24018778317593756No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA870.23745837654893828No Hit
TCGTAAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA870.23745837654893828No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCGGACTGCTCTTCCGA790.21562312353294394No Hit
TCGTAAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC790.21562312353294394No Hit
TCGTAAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG750.2047054970249468No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCG700.19105846388995032No Hit
TCGTAAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT700.19105846388995032No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA670.18287024400895244No Hit
TCGTAAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA650.17741143075495389No Hit
TCGTAAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA650.17741143075495389No Hit
TCGTAAGGGATATGGTCTGTGACTATAGCTGACGTCTTCTGCAGAGGCAGTATGTTCA640.17468202412795458No Hit
TCGTAAGGGATAGAACAAGAGAGAATCATCTCTAACTTATTTTATGAGGCCAGAATCA610.1664938042469567No Hit
TCGTAAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC610.1664938042469567No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCG610.1664938042469567No Hit
TCGTAAGGGAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTG590.16103499099295815No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC580.15830558436595885No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG580.15830558436595885No Hit
TCGTAAGGGGGTGGCGCGGAGAACTCTGCAAAACAAGAGGCTGGGGATTGCGTTAGCG580.15830558436595885No Hit
TCGTAAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA570.15557617773895954No Hit
TCGTAAGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCCG570.15557617773895954No Hit
TCGTAAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA540.14738795785796166No Hit
TCGTAAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGTCC530.1446585512309624No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT530.1446585512309624No Hit
TCGTAAGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGT520.1419291446039631No Hit
TCGTAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA520.1419291446039631No Hit
TCGTAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA500.13647033134996453No Hit
TCGTAAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA490.13374092472296523No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA490.13374092472296523No Hit
TCGTAAGGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGTCA480.13101151809596592No Hit
TCGTAAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA480.13101151809596592No Hit
TCGTAAGGGGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA480.13101151809596592No Hit
TCGTAAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA480.13101151809596592No Hit
TCGTAAGGGACCCCATTGTTTCAAAGCTCCAGTTAAAGAGGGCCACATGTACTGCTCC470.12828211146896665No Hit
TCGTAAGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAATTCTCAG460.12555270484196737No Hit
TCGTAAGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGG460.12555270484196737No Hit
TCGTAAGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGGTC450.12282329821496807No Hit
TCGTAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.12009389158796878No Hit
TCGTAAGGGGCTCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC440.12009389158796878No Hit
TCGTAAGGGAGCTAATTGCCAAATTACAGATGAGTTACTCCCACAGTTTATTTATTTT430.11736448496096949No Hit
TCGTAAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG430.11736448496096949No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCGA430.11736448496096949No Hit
TCGTAAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC430.11736448496096949No Hit
TCGTAAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT420.11463507833397019No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCCGA420.11463507833397019No Hit
TCGTAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.11463507833397019No Hit
TCGTAAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTGGC420.11463507833397019No Hit
TCGTAAGGGGGGGCTGATGAGATGGCTCAACGGTTAAGAGCACTGATTGCTCTTCTGA410.1119056717069709No Hit
TCGTAAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGGCC410.1119056717069709No Hit
TCGTAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCAG410.1119056717069709No Hit
TCGTAAGGGGCCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAGACCC410.1119056717069709No Hit
TCGTAAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT400.10917626507997162No Hit
TCGTAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA390.10644685845297233No Hit
TCGTAAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC390.10644685845297233No Hit
TCGTAAGGGACCATAGCCAGTGCCAGCACGGCCTCCAGCATTCCCAGGCCTGCGCACT390.10644685845297233No Hit
TCGTAAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA380.10371745182597303No Hit
TCGTAAGGGGCCTTTTTTTCCCTCAGCGCTGGCCGCGGCCTCCCGGCGCTCTCGTCCT380.10371745182597303No Hit
TCGTAAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG380.10371745182597303No Hit
TCGTAAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGGGC380.10371745182597303No Hit
TCGTAAGGGGGCACGTGCTGCTCCGGCTCCCGCGTGGCGTTCTCAGCATCCAGTCTTC370.10098804519897374No Hit
TCGTAAGGGGTCAGACAAGCCCGGCAGCCCAGGAGCCATGGCGTCTTATTTTGATGAG370.10098804519897374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAG307.7556433E-752.02599339
GAGCGCA353.731475E-852.02599343
CCTATCG251.618816E-552.02599347
ATCTCAT150.00715048652.02599349
AGCTCAA150.00715048652.02599325
TTCGCTA503.6379788E-1252.02599333
ACATGAT150.00715048652.02599331
GATACAT150.00715048652.02599322
CACAATT150.00715048652.02599342
TTATCTC150.00715048652.02599347
TCGGTGT150.00715048652.02599351
TGATTTA150.00715048652.02599334
TTCTGTC150.00715048652.02599320
TATCGGT150.00715048652.02599349
TTATCAC150.00715048652.02599338
ACCGTAG251.618816E-552.02599339
GTTTCGC251.618816E-552.02599331
GGTTCGC353.731475E-852.02599331
CAATTAT150.00715048652.02599344
GGTTTCG251.618816E-552.02599330
Produced by FastQC (version 0.11.5)