##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212572_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 36638 Sequences flagged as poor quality 0 Sequence length 58 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.92414978983569 39.0 38.0 39.0 35.0 40.0 2 37.10682897538075 39.0 36.0 39.0 33.0 40.0 3 36.743353894863255 38.0 35.0 39.0 31.0 40.0 4 36.56880834106665 38.0 35.0 39.0 31.0 40.0 5 37.3199956329494 39.0 36.0 39.0 33.0 40.0 6 37.82152410066052 39.0 38.0 39.0 35.0 40.0 7 36.903570063868116 38.0 36.0 39.0 33.0 40.0 8 37.826682679185545 39.0 38.0 39.0 35.0 40.0 9 37.80370107538621 39.0 38.0 39.0 35.0 40.0 10 37.063868115071784 38.0 36.0 39.0 33.0 40.0 11 36.879660461815604 38.0 36.0 39.0 33.0 40.0 12 36.57470385938097 38.0 35.0 39.0 31.0 40.0 13 36.295294502975054 38.0 35.0 39.0 31.0 40.0 14 36.5421147442546 38.0 35.0 39.0 31.0 40.0 15 36.50390305147661 38.0 35.0 39.0 31.0 40.0 16 36.213958185490476 38.0 35.0 39.0 30.0 40.0 17 35.4625252470113 38.0 34.0 39.0 29.0 40.0 18 35.59861346143349 38.0 35.0 39.0 29.0 40.0 19 35.21035536874284 38.0 33.0 39.0 28.0 40.0 20 35.249686118237896 38.0 33.0 39.0 28.0 40.0 21 34.739587313718 38.0 33.0 39.0 26.0 40.0 22 34.64812489764725 38.0 33.0 39.0 26.0 40.0 23 34.50316611168732 38.0 33.0 39.0 26.0 40.0 24 34.528003711993016 37.0 33.0 39.0 27.0 40.0 25 34.38683880124461 37.0 33.0 39.0 26.0 40.0 26 34.12882799279436 38.0 33.0 39.0 25.0 40.0 27 33.87087177247666 37.0 33.0 39.0 25.0 40.0 28 33.62162235929909 36.0 32.0 39.0 23.0 40.0 29 33.440280583001254 36.0 32.0 38.0 23.0 40.0 30 33.32048692614226 36.0 32.0 38.0 23.0 40.0 31 32.45709372782357 36.0 30.0 38.0 20.0 39.0 32 32.135132922102734 36.0 30.0 38.0 19.0 39.0 33 32.71049183907419 36.0 31.0 38.0 22.0 39.0 34 31.311043179212838 35.0 29.0 38.0 16.0 39.0 35 30.472296522735956 35.0 27.0 38.0 12.0 39.0 36 31.80531142529614 36.0 30.0 38.0 16.0 40.0 37 31.483377913641576 36.0 30.0 38.0 15.0 40.0 38 31.129646814782465 35.0 29.0 38.0 12.0 39.0 39 31.141656203941263 35.0 29.0 38.0 13.0 40.0 40 30.784294994268247 35.0 29.0 38.0 10.0 39.0 41 31.473115344724057 36.0 30.0 39.0 7.0 40.0 42 31.19378787051695 36.0 30.0 39.0 2.0 40.0 43 31.013565150936188 36.0 29.0 39.0 2.0 40.0 44 31.11851083574431 36.0 30.0 39.0 2.0 40.0 45 30.6654566297287 35.0 29.0 38.0 2.0 39.0 46 29.985233910147933 35.0 28.0 38.0 2.0 39.0 47 29.515011736448496 35.0 27.0 38.0 2.0 39.0 48 29.66589333478902 35.0 28.0 38.0 2.0 39.0 49 29.405890059501065 35.0 27.0 38.0 2.0 39.0 50 29.22282875702822 35.0 27.0 38.0 2.0 39.0 51 29.011545390032207 35.0 27.0 38.0 2.0 39.0 52 28.73254544462034 35.0 26.0 38.0 2.0 39.0 53 28.42726131339047 35.0 26.0 38.0 2.0 39.0 54 28.285905344178175 35.0 25.0 38.0 2.0 39.0 55 27.96257983514384 35.0 24.0 38.0 2.0 39.0 56 27.717970413232162 35.0 23.0 38.0 2.0 39.0 57 27.445548337791365 35.0 21.0 38.0 2.0 39.0 58 26.740515311971176 34.0 19.0 38.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.3469294899297637 1 2 -4.850146090111146 1 3 0.49208833385483075 1 4 -4.7955931438114305 1 5 -3.310642844321663 1 6 -3.125288118288367 1 7 -5.914302683641637 1 8 -3.075773589246566 1 9 -3.0278798396373503 1 10 -3.2116029775892443 1 11 -3.708507044168755 1 12 -3.2588739556445887 1 13 1.1552066456877412 1 14 0.6573779985450585 1 15 -3.2786981631507714 1 16 -15.534926662641247 1 17 -6.487127508757482 1 18 -7.837674544642844 1 19 -1.1705780697328905 1 20 -3.1273652241036842 1 21 2.6102239187846408 1 22 -8.941832518204738 1 23 -1.8574103329164835 1 24 -1.8415941598975536 1 25 -1.500276528765614 1 26 -7.932430612061825 1 27 -9.643448423562258 1 28 -8.276694007081279 1 29 -6.077598377414983 1 30 0.9332896394304946 1 31 -3.629177200435766 1 32 1.5301912897073926 1 33 1.0823137010676298 1 34 2.723767201759717 1 35 8.414937528085524 1 36 1.0033271642771169 1 37 -11.581172448205134 1 38 1.9605627339755856 1 39 4.717894422806708 1 40 -0.490737939893652 1 41 5.588073346814831 1 42 2.9855146754039374 1 43 8.950960094726938 1 44 1.8803337631524535 1 45 3.2179960917825596 1 46 -23.812767401595618 1 47 -23.455373867471337 1 48 -23.686849234661892 1 49 -23.237640361360395 1 50 -23.26279379737039 1 51 -22.74247477578501 1 52 -22.403172645557166 1 53 -22.060043989591342 1 54 -21.918843919092566 1 55 -21.637756612589573 1 56 -21.15910229764351 1 57 -20.883171206517662 1 58 -20.146153736525022 2 1 0.6530705100702363 2 2 -0.8501460901111457 2 3 -0.5079116661451692 2 4 3.2044068561885695 2 5 -1.310642844321663 2 6 -2.125288118288367 2 7 -3.914302683641637 2 8 -2.075773589246566 2 9 -2.0278798396373503 2 10 -3.2116029775892443 2 11 -14.708507044168755 2 12 -9.258873955644589 2 13 -8.844793354312259 2 14 -9.342622001454941 2 15 -5.2786981631507714 2 16 -1.5349266626412472 2 17 -3.4871275087574816 2 18 -0.8376745446428444 2 19 -0.17057806973289047 2 20 -10.127365224103684 2 21 -10.38977608121536 2 22 -12.941832518204738 2 23 -14.857410332916483 2 24 -6.841594159897554 2 25 -9.500276528765614 2 26 3.0675693879381747 2 27 -1.6434484235622584 2 28 -1.2766940070812787 2 29 -6.077598377414983 2 30 -1.0667103605695054 2 31 -2.629177200435766 2 32 -0.4698087102926074 2 33 1.0823137010676298 2 34 5.723767201759717 2 35 3.414937528085524 2 36 7.003327164277117 2 37 2.418827551794866 2 38 -4.039437266024414 2 39 5.717894422806708 2 40 -17.490737939893652 2 41 -8.41192665318517 2 42 -17.014485324596063 2 43 -5.049039905273062 2 44 -5.1196662368475465 2 45 -4.78200390821744 2 46 9.187232598404382 2 47 9.544626132528663 2 48 12.313150765338108 2 49 -14.237640361360395 2 50 9.737206202629611 2 51 -22.74247477578501 2 52 -22.403172645557166 2 53 -22.060043989591342 2 54 -21.918843919092566 2 55 -21.637756612589573 2 56 -21.15910229764351 2 57 -20.883171206517662 2 58 -20.146153736525022 3 1 -0.6802628232630994 3 2 -1.1834794234444814 3 3 -2.841244999478505 3 4 -2.462259810478095 3 5 -0.3106428443216629 3 6 0.874711881711633 3 7 -5.247636016974969 3 8 -0.07577358924656608 3 9 -0.0278798396373503 3 10 0.7883970224107557 3 11 1.9581596224979094 3 12 -0.925540622311253 3 13 1.488539979021077 3 14 -2.3426220014549415 3 15 0.7213018368492286 3 16 -2.534926662641247 3 17 -9.487127508757482 3 18 -5.837674544642844 3 19 -5.503911403066223 3 20 -3.1273652241036842 3 21 -12.38977608121536 3 22 -11.27516585153807 3 23 -14.190743666249816 3 24 -10.508260826564221 3 25 -12.166943195432282 3 26 -9.599097278728493 3 27 -8.643448423562258 3 28 -6.610027340414611 3 29 -12.74426504408165 3 30 -8.066710360569505 3 31 -7.629177200435766 3 32 -9.80314204362594 3 33 -11.584352965599038 3 34 -4.609566131573615 3 35 -9.251729138581144 3 36 -13.66333950238955 3 37 -10.247839114871802 3 38 -16.37277059935775 3 39 -9.615438910526624 3 40 -7.490737939893652 3 41 -16.078593319851834 3 42 -10.68115199126273 3 43 -10.382373238606394 3 44 -11.119666236847547 3 45 -6.448670574884108 3 46 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4.172401622585017 4 30 3.9332896394304946 4 31 -2.629177200435766 4 32 -8.719808710292607 4 33 -3.66768629893237 4 34 -2.776232798240283 4 35 -4.335062471914476 4 36 -5.246672835722883 4 37 -5.331172448205134 4 38 -5.039437266024414 4 39 -8.532105577193292 4 40 -2.990737939893652 4 41 -3.6619266531851693 4 42 -2.0144853245960626 4 43 -0.7990399052730623 4 44 -4.1196662368475465 4 45 -0.03200390821744037 4 46 -1.562767401595618 4 47 -0.45537386747133723 4 48 -1.9368492346618922 4 49 -2.237640361360395 4 50 -0.7627937973703887 4 51 0.7575252242149908 4 52 1.0968273544428335 4 53 1.1899560104086575 4 54 -1.4188439190925664 4 55 -2.8877566125895733 4 56 1.8408977023564894 4 57 -4.6331712065176625 4 58 -3.3961537365250223 5 1 -0.5969294899297637 5 2 -1.6001460901111457 5 3 -1.2579116661451692 5 4 -3.2955931438114305 5 5 -0.5606428443216629 5 6 -0.37528811828836695 5 7 -2.914302683641637 5 8 -0.3257735892465661 5 9 -0.2778798396373503 5 10 -0.2116029775892443 5 11 -2.208507044168755 5 12 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46 8.187232598404382 120 47 8.544626132528663 120 48 12.313150765338108 120 49 10.762359638639605 120 50 6.737206202629611 120 51 5.257525224214991 120 52 8.596827354442834 120 53 -1.0600439895913425 120 54 11.081156080907434 120 55 11.362243387410427 120 56 -4.159102297643511 120 57 5.1168287934823375 120 58 0.8538462634749777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25.0 3 0.0 4 5.0 5 15.0 6 14.0 7 32.0 8 37.0 9 58.0 10 105.0 11 97.0 12 145.0 13 165.0 14 177.0 15 229.0 16 243.0 17 269.0 18 269.0 19 285.0 20 350.0 21 399.0 22 506.0 23 493.0 24 541.0 25 573.0 26 692.0 27 775.0 28 902.0 29 1104.0 30 1252.0 31 1449.0 32 1736.0 33 2064.0 34 2465.0 35 3000.0 36 3623.0 37 4788.0 38 4756.0 39 2996.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 73.34461488072493 17.473661226049455 2.339101479338392 6.842622413887221 11 40.13319504339756 17.050603198864568 13.930891424204377 28.885310333533486 12 41.1840165947923 9.673017086085485 26.106774387248212 23.03619193187401 13 23.663955456083848 15.99978164746984 29.76417926742726 30.572083629019055 14 16.895027021125607 19.362410611932965 43.774223483814616 19.96833888312681 15 24.594683115890607 13.4668922976145 41.62618046836617 20.31224411812872 16 29.189071157572943 12.541965772306686 37.358408166607525 20.910554903512843 17 27.335007369397896 14.41399639718325 24.84578852557454 33.40520770784431 18 31.04154156886293 14.525902068890224 25.590916534745347 28.841639827501503 19 26.212638187525588 21.605022519448614 31.570902142759653 20.61143715026614 20 34.30591189475408 18.903870298597088 25.214258420219444 21.575959386429393 21 28.186582237021675 21.22386593154648 25.86658660407227 24.722965227359573 22 32.92210273486544 18.81379987990611 25.53086958895136 22.733227796277088 23 37.60406146791495 19.562191227447663 25.021153478723697 17.812593825913694 24 38.924067907636875 16.717615590370656 21.45859490146842 22.899721600524046 25 30.323707625962115 22.07544079917026 21.988099787106282 25.61275178776134 26 28.91151819596495 16.743563842856755 25.763739113816918 28.581178847361382 27 20.786700878964893 15.545667958726867 25.724736583501667 37.94289457880657 28 21.476931476931476 27.242697242697243 22.63991263991264 28.64045864045864 29 31.858141312656983 18.30020749153653 26.63809107786393 23.20356011794256 30 26.535572852656014 16.506896080841184 29.403250034139017 27.554281032363786 31 32.47207276104116 14.93458607598394 28.71657607953459 23.876765083440308 32 41.073037542662114 12.922866894197952 29.26689419795222 16.737201365187712 33 41.75266175266175 11.613431613431613 26.238056238056238 20.395850395850395 34 32.811860103202555 13.735768695224834 36.115433969476065 17.336937232096542 35 26.173378839590445 23.421160409556315 35.500341296928326 14.905119453924915 36 29.01305368944235 31.137691845540445 25.23895352012671 14.610300944890492 37 32.88995711905607 23.122934476825172 24.909185262065385 19.07792314205337 38 25.05736451048951 29.318728146853147 26.852054195804197 18.771853146853147 39 34.84922976073418 22.51993881787392 23.847372446192505 18.783458975199387 40 26.098000655522778 24.945373101715283 24.931716377144106 24.02490986561783 41 26.980962005954495 20.843462347381934 27.57368004151758 24.601895605145994 42 24.64005682594323 22.618364614922275 26.077097505668934 26.66448105346556 43 23.612818621424474 18.599568341392782 27.33655711280496 30.451055924377783 44 24.855917620387316 15.085630001911992 27.890524705689547 32.167927672011146 45 29.88555977385082 14.683309207112227 25.589271570207305 29.84185944882965 46 27.69340034965035 23.07965472027972 25.76486013986014 23.46208479020979 47 24.64409651064295 19.856818864934283 31.671994972265487 23.82708965215728 48 25.138657413731863 19.231714980465014 32.23409196470042 23.3955356411027 49 28.688546989861447 19.129341677369986 30.612412209985518 21.569699122783046 50 21.604634639558398 18.500300595726074 31.58441274525878 28.31065201945674 51 22.18487854195699 15.875618220072685 34.54654753121841 27.39295570675192 52 18.972159230622115 18.25359962842545 34.7722739815852 28.00196715936723 53 17.177763201049526 18.04416748660763 40.264567617798185 24.513501694544658 54 20.746672497198613 19.385607696302166 34.62516056738364 25.24255923911558 55 20.630450829756406 16.215107854662765 34.08699455942259 29.067446756158244 56 20.55614797801657 19.648374483908896 25.87154458206874 33.9239329560058 57 24.221377594268684 19.242022367449618 25.413579065379672 31.123020972902026 58 22.233769075097083 27.284909478750752 26.88016189903189 23.601159547120275 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.5 9 15.0 10 25.0 11 23.0 12 21.0 13 34.5 14 48.0 15 100.5 16 220.0 17 287.0 18 246.5 19 206.0 20 231.5 21 257.0 22 290.0 23 412.0 24 501.0 25 567.0 26 633.0 27 656.0 28 659.0 29 639.0 30 543.0 31 447.0 32 492.5 33 538.0 34 462.5 35 362.0 36 337.0 37 307.5 38 278.0 39 298.0 40 318.0 41 373.5 42 424.5 43 420.0 44 766.5 45 1113.0 46 968.0 47 918.0 48 1013.0 49 971.0 50 929.0 51 1196.5 52 1464.0 53 1454.5 54 1385.0 55 1325.0 56 1743.5 57 2162.0 58 2863.0 59 3855.5 60 4147.0 61 4216.0 62 4285.0 63 3535.0 64 2785.0 65 2355.0 66 1500.5 67 1076.0 68 850.5 69 625.0 70 513.0 71 401.0 72 332.5 73 217.0 74 170.0 75 136.5 76 103.0 77 68.0 78 33.0 79 33.0 80 22.0 81 11.0 82 8.5 83 6.0 84 3.5 85 2.5 86 4.0 87 2.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0027294066269992904 17 0.0 18 0.0 19 0.00818821988099787 20 0.0 21 0.0 22 0.0 23 0.0027294066269992904 24 0.0 25 0.0 26 0.024564659642993612 27 0.010917626507997161 28 0.021835253015994323 29 0.027294066269992905 30 0.06277635242098369 31 0.06823516567498226 32 0.03548228615099078 33 0.021835253015994323 34 0.030023472896992195 35 0.03548228615099078 36 0.05458813253998581 37 0.06823516567498226 38 0.08188219880997871 39 0.07096457230198155 40 0.07096457230198155 41 0.07369397892898083 42 0.09552923194497516 43 0.09552923194497516 44 0.07369397892898083 45 0.06823516567498226 46 0.08188219880997871 47 0.1119056717069709 48 0.10098804519897374 49 0.12282329821496807 50 0.12009389158796878 51 0.1119056717069709 52 0.10098804519897374 53 0.13647033134996453 54 0.13374092472296523 55 0.1664938042469567 56 0.17741143075495389 57 0.18287024400895244 58 0.1965172771439489 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 36638.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.99748894590316 #Duplication Level Percentage of deduplicated Percentage of total 1 68.95472061657033 23.442873519296906 2 12.508028259473347 8.504831049729788 3 5.41907514450867 5.527048419673563 4 2.9784842646114322 4.050439434466947 5 2.159601798330122 3.6710519133140456 6 1.3407193320488118 2.734865440253289 7 1.2443802183686576 2.9614061902942304 8 0.8831085420680796 2.4018778317593754 9 0.5700064226075787 1.7440908346525466 >10 3.5324341682723186 22.96249795294503 >50 0.23281952472703915 5.4233309678475905 >100 0.1445086705202312 8.439325290681806 >500 0.024084778420038536 5.270484196735629 >1k 0.008028259473346178 2.865876958349255 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 1050 2.865876958349255 No Hit TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 821 2.2408428407664176 No Hit TCGTAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 601 1.6403733828265734 No Hit TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 509 1.3892679731426387 No Hit TCGTAAGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 357 0.9743981658387466 No Hit TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 318 0.8679513073857743 No Hit TCGTAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 283 0.7724220754407991 No Hit TCGTAAGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTG 222 0.6059282711938425 No Hit TCGTAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 201 0.5486107320268574 No Hit TCGTAAGGGGGGTTAGAGGGGGAAGGGGTTGTGTGTTTTCTTTTTTTGAGACAAGGCT 177 0.4831049729788744 No Hit TCGTAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA 169 0.46126971996288 No Hit TCGTAAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC 154 0.42032862055789066 No Hit TCGTAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 150 0.4094109940498936 No Hit TCGTAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 145 0.3957639609148971 No Hit TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT 136 0.3711993012719035 No Hit TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 120 0.32752879523991485 No Hit TCGTAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 117 0.31934057535891697 No Hit TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG 113 0.3084229488509198 No Hit TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA 111 0.30296413559692126 No Hit TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT 107 0.29204650908892404 No Hit TCGTAAGGGGTGGTATCAACGCAGAATACAGTGCTTCGTAAGGGGGGGCTGGTGAGAT 106 0.2893171024619248 No Hit TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA 106 0.2893171024619248 No Hit TCGTAAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC 100 0.27294066269992906 No Hit TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 99 0.2702112560729298 No Hit TCGTAAGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC 98 0.26748184944593045 No Hit TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT 88 0.24018778317593756 No Hit TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA 87 0.23745837654893828 No Hit TCGTAAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA 87 0.23745837654893828 No Hit TCGTAAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCGGACTGCTCTTCCGA 79 0.21562312353294394 No Hit TCGTAAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC 79 0.21562312353294394 No Hit TCGTAAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG 75 0.2047054970249468 No Hit TCGTAAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCG 70 0.19105846388995032 No Hit TCGTAAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT 70 0.19105846388995032 No Hit TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA 67 0.18287024400895244 No Hit TCGTAAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 65 0.17741143075495389 No Hit TCGTAAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA 65 0.17741143075495389 No Hit TCGTAAGGGATATGGTCTGTGACTATAGCTGACGTCTTCTGCAGAGGCAGTATGTTCA 64 0.17468202412795458 No Hit TCGTAAGGGATAGAACAAGAGAGAATCATCTCTAACTTATTTTATGAGGCCAGAATCA 61 0.1664938042469567 No Hit TCGTAAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC 61 0.1664938042469567 No Hit TCGTAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCG 61 0.1664938042469567 No Hit TCGTAAGGGAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTG 59 0.16103499099295815 No Hit TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 58 0.15830558436595885 No Hit TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG 58 0.15830558436595885 No Hit TCGTAAGGGGGTGGCGCGGAGAACTCTGCAAAACAAGAGGCTGGGGATTGCGTTAGCG 58 0.15830558436595885 No Hit TCGTAAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA 57 0.15557617773895954 No Hit TCGTAAGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCCG 57 0.15557617773895954 No Hit TCGTAAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA 54 0.14738795785796166 No Hit TCGTAAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGTCC 53 0.1446585512309624 No Hit TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 53 0.1446585512309624 No Hit TCGTAAGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGT 52 0.1419291446039631 No Hit TCGTAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 52 0.1419291446039631 No Hit TCGTAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA 50 0.13647033134996453 No Hit TCGTAAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA 49 0.13374092472296523 No Hit TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA 49 0.13374092472296523 No Hit TCGTAAGGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGTCA 48 0.13101151809596592 No Hit TCGTAAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA 48 0.13101151809596592 No Hit TCGTAAGGGGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA 48 0.13101151809596592 No Hit TCGTAAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA 48 0.13101151809596592 No Hit TCGTAAGGGACCCCATTGTTTCAAAGCTCCAGTTAAAGAGGGCCACATGTACTGCTCC 47 0.12828211146896665 No Hit TCGTAAGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAATTCTCAG 46 0.12555270484196737 No Hit TCGTAAGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGG 46 0.12555270484196737 No Hit TCGTAAGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGGTC 45 0.12282329821496807 No Hit TCGTAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44 0.12009389158796878 No Hit TCGTAAGGGGCTCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC 44 0.12009389158796878 No Hit TCGTAAGGGAGCTAATTGCCAAATTACAGATGAGTTACTCCCACAGTTTATTTATTTT 43 0.11736448496096949 No Hit TCGTAAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG 43 0.11736448496096949 No Hit TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCGA 43 0.11736448496096949 No Hit TCGTAAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC 43 0.11736448496096949 No Hit TCGTAAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 42 0.11463507833397019 No Hit TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCCGA 42 0.11463507833397019 No Hit TCGTAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42 0.11463507833397019 No Hit TCGTAAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTGGC 42 0.11463507833397019 No Hit TCGTAAGGGGGGGCTGATGAGATGGCTCAACGGTTAAGAGCACTGATTGCTCTTCTGA 41 0.1119056717069709 No Hit TCGTAAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGGCC 41 0.1119056717069709 No Hit TCGTAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCAG 41 0.1119056717069709 No Hit TCGTAAGGGGCCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAGACCC 41 0.1119056717069709 No Hit TCGTAAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT 40 0.10917626507997162 No Hit TCGTAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA 39 0.10644685845297233 No Hit TCGTAAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC 39 0.10644685845297233 No Hit TCGTAAGGGACCATAGCCAGTGCCAGCACGGCCTCCAGCATTCCCAGGCCTGCGCACT 39 0.10644685845297233 No Hit TCGTAAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA 38 0.10371745182597303 No Hit TCGTAAGGGGCCTTTTTTTCCCTCAGCGCTGGCCGCGGCCTCCCGGCGCTCTCGTCCT 38 0.10371745182597303 No Hit TCGTAAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG 38 0.10371745182597303 No Hit TCGTAAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGGGC 38 0.10371745182597303 No Hit TCGTAAGGGGGCACGTGCTGCTCCGGCTCCCGCGTGGCGTTCTCAGCATCCAGTCTTC 37 0.10098804519897374 No Hit TCGTAAGGGGTCAGACAAGCCCGGCAGCCCAGGAGCCATGGCGTCTTATTTTGATGAG 37 0.10098804519897374 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.005458813253998581 0.0 0.0 0.0 0.0 11 0.008188219880997872 0.0 0.0 0.0 0.0 12 0.008188219880997872 0.0 0.0 0.0 0.0 13 0.008188219880997872 0.0 0.0 0.0 0.0 14 0.010917626507997161 0.0 0.0 0.0 0.0 15 0.010917626507997161 0.0 0.0 0.0 0.0 16 0.010917626507997161 0.0 0.0 0.0 0.0 17 0.013647033134996453 0.0 0.0 0.0 0.0 18 0.013647033134996453 0.0 0.0 0.0 0.0 19 0.013647033134996453 0.0 0.0 0.0 0.0 20 0.019105846388995033 0.0 0.0 0.0 0.0 21 0.021835253015994323 0.0 0.0 0.0 0.0 22 0.021835253015994323 0.0 0.0 0.0 0.0 23 0.024564659642993612 0.0 0.0 0.0 0.0 24 0.024564659642993612 0.0 0.0 0.0 0.0 25 0.024564659642993612 0.0 0.0 0.0 0.0 26 0.024564659642993612 0.0 0.0 0.0 0.0 27 0.024564659642993612 0.0 0.0 0.0 0.0 28 0.027294066269992905 0.0 0.0 0.0 0.0 29 0.030023472896992195 0.0 0.0 0.0 0.0 30 0.03275287952399149 0.0 0.0 0.0 0.0 31 0.03548228615099078 0.0 0.0 0.0 0.0 32 0.03548228615099078 0.0 0.0 0.0 0.0 33 0.03821169277799007 0.0 0.0 0.0 0.0 34 0.03821169277799007 0.0 0.0 0.0 0.0 35 0.051858725912986514 0.0 0.0 0.0 0.0 36 0.05458813253998581 0.0 0.0 0.0 0.0 37 0.05458813253998581 0.0 0.0 0.0 0.0 38 0.05458813253998581 0.0 0.0 0.0 0.0 39 0.05458813253998581 0.0 0.0 0.0 0.0 40 0.05458813253998581 0.0 0.0 0.0 0.0 41 0.0573175391669851 0.0 0.0 0.0 0.0 42 0.0573175391669851 0.0 0.0 0.0 0.0 43 0.0573175391669851 0.0 0.0 0.0 0.0 44 0.0573175391669851 0.0 0.0 0.0 0.0 45 0.0573175391669851 0.0 0.0 0.0 0.0 46 0.06823516567498226 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGGAG 30 7.7556433E-7 52.025993 39 GAGCGCA 35 3.731475E-8 52.025993 43 CCTATCG 25 1.618816E-5 52.025993 47 ATCTCAT 15 0.007150486 52.025993 49 AGCTCAA 15 0.007150486 52.025993 25 TTCGCTA 50 3.6379788E-12 52.025993 33 ACATGAT 15 0.007150486 52.025993 31 GATACAT 15 0.007150486 52.025993 22 CACAATT 15 0.007150486 52.025993 42 TTATCTC 15 0.007150486 52.025993 47 TCGGTGT 15 0.007150486 52.025993 51 TGATTTA 15 0.007150486 52.025993 34 TTCTGTC 15 0.007150486 52.025993 20 TATCGGT 15 0.007150486 52.025993 49 TTATCAC 15 0.007150486 52.025993 38 ACCGTAG 25 1.618816E-5 52.025993 39 GTTTCGC 25 1.618816E-5 52.025993 31 GGTTCGC 35 3.731475E-8 52.025993 31 CAATTAT 15 0.007150486 52.025993 44 GGTTTCG 25 1.618816E-5 52.025993 30 >>END_MODULE