FastQCFastQC Report
Tue 31 May 2016
SRR212571_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212571_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39208
Sequences flagged as poor quality0
Sequence length58
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT11102.8310548867578045No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA8442.1526219138951235No Hit
TCGTAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC6771.7266884309324628No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5941.5149969394001226No Hit
TCGTAAGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3570.9105284635788614No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3290.8391144664354213No Hit
TCGTAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG2870.7319934707202612No Hit
TCGTAAGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTG2330.5942664762293409No Hit
TCGTAAGGGGGGTTAGAGGGGGAAGGGGTTGTGTGTTTTCTTTTTTTGAGACAAGGCT2190.5585594776576209No Hit
TCGTAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1850.4718424811263008No Hit
TCGTAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1810.46164048153438075No Hit
TCGTAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1750.4463374821465007No Hit
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1610.41063048357478066No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1350.3443174862273005No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1340.34176698632932057No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1290.32901448683942053No Hit
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT1270.3239134870434605No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG1260.3213629871454805No Hit
TCGTAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1250.3188124872475005No Hit
TCGTAAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC1220.3111609875535605No Hit
TCGTAAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC1090.27800448887982043No Hit
TCGTAAGGGGTGGTATCAACGCAGAATACAGTGCTTCGTAAGGGGGGGCTGGTGAGAT1060.2703529891858804No Hit
TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.2576004896959804No Hit
TCGTAAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA990.2524994899000204No Hit
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT970.2473984901040604No Hit
TCGTAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC920.23464599061416036No Hit
TCGTAAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG890.2269944909202204No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA860.21934299122628037No Hit
TCGTAAGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC850.21679249132830034No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCGGACTGCTCTTCCGA840.21424199143032036No Hit
TCGTAAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC760.19383799224648032No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA760.19383799224648032No Hit
TCGTAAGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGT760.19383799224648032No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCG690.17598449296062027No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG690.17598449296062027No Hit
TCGTAAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT680.1734339930626403No Hit
TCGTAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA670.17088349316466028No Hit
TCGTAAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA670.17088349316466028No Hit
TCGTAAGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCCG620.15813099367476025No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC610.15558049377678024No Hit
TCGTAAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC610.15558049377678024No Hit
TCGTAAGGGATAGAACAAGAGAGAATCATCTCTAACTTATTTTATGAGGCCAGAATCA600.15302999387880023No Hit
TCGTAAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA600.15302999387880023No Hit
TCGTAAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA590.15047949398082025No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA590.15047949398082025No Hit
TCGTAAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGTCC580.14792899408284024No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT570.14537849418486024No Hit
TCGTAAGGGAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTG550.14027749438890022No Hit
TCGTAAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGGCC530.1351764945929402No Hit
TCGTAAGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGGTC530.1351764945929402No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCGA530.1351764945929402No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCG530.1351764945929402No Hit
TCGTAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA530.1351764945929402No Hit
TCGTAAGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC510.13007549479698022No Hit
TCGTAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.1275249948990002No Hit
TCGTAAGGGAGCTAATTGCCAAATTACAGATGAGTTACTCCCACAGTTTATTTATTTT480.1224239951030402No Hit
TCGTAAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGCCC480.1224239951030402No Hit
TCGTAAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG470.11987349520506019No Hit
TCGTAAGGGGGGGCTGATGAGATGGCTCAACGGTTAAGAGCACTGATTGCTCTTCTGA460.11732299530708018No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCGA460.11732299530708018No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC460.11732299530708018No Hit
TCGTAAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA460.11732299530708018No Hit
TCGTAAGGGATATGGTCTGTGACTATAGCTGACGTCTTCTGCAGAGGCAGTATGTTCA460.11732299530708018No Hit
TCGTAAGGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGTCA450.11477249540910019No Hit
TCGTAAGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC450.11477249540910019No Hit
TCGTAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.11477249540910019No Hit
TCGTAAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC450.11477249540910019No Hit
TCGTAAGGGACCATAGCCAGTGCCAGCACGGCCTCCAGCATTCCCAGGCCTGCGCACT450.11477249540910019No Hit
TCGTAAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA450.11477249540910019No Hit
TCGTAAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA440.11222199551112018No Hit
TCGTAAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT440.11222199551112018No Hit
TCGTAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA440.11222199551112018No Hit
TCGTAAGGGGAAGTGGAGCTAGCCATAAACCTCAAGGCTTGTTTCCCAGAGATCCACT440.11222199551112018No Hit
TCGTAAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC440.11222199551112018No Hit
TCGTAAGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA440.11222199551112018No Hit
TCGTAAGGGGCCTTTTTTTCCCTCAGCGCTGGCCGCGGCCTCCCGGCGCTCTCGTCCT430.10967149561314019No Hit
TCGTAAGGGACCCCATTGTTTCAAAGCTCCAGTTAAAGAGGGCCACATGTACTGCTCC430.10967149561314019No Hit
TCGTAAGGGGCTCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC430.10967149561314019No Hit
TCGTAAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT430.10967149561314019No Hit
TCGTAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA420.10712099571516018No Hit
TCGTAAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCCC420.10712099571516018No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCCGA410.10457049581718016No Hit
TCGTAAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG410.10457049581718016No Hit
TCGTAAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA410.10457049581718016No Hit
TCGTAAGGGGGTGGCGCGGAGAACTCTGCAAAACAAGAGGCTGGGGATTGCGTTAGCG410.10457049581718016No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCA410.10457049581718016No Hit
TCGTAAGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAATTCTCAG400.10201999591920018No Hit
TCGTAAGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGG400.10201999591920018No Hit
TCGTAAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA400.10201999591920018No Hit
TCGTAAGGGGCTTTCGACCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATGACGAAC400.10201999591920018No Hit
TCGTAAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA400.10201999591920018No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTTCC400.10201999591920018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCCA251.5884361E-552.20384652
ATATGGG150.00705817852.20384652
AAAACCA203.3413235E-452.20384652
CCAACTG203.3625777E-452.13700543
GTGTTGT150.007094096452.13700544
CAGCATC150.007094096452.13700543
CCTATCG251.6005655E-552.13700547
CAGCACT150.007094096452.13700541
ACGTTTC150.007094096452.13700540
GCAGGTG150.007094096452.13700540
CAACTGC203.3625777E-452.13700544
TATCGGG401.7735147E-952.13700549
CGTAGCG251.6005655E-552.13700541
AGGTGTT150.007094096452.13700542
TCAGCAT150.007094096452.13700542
CCGTAGC251.6005655E-552.13700540
CCCAACT203.3625777E-452.13700542
CACCCAA203.3625777E-452.13700540
ATCGGGG401.7735147E-952.13700550
AGGCTAC251.6005655E-552.13700551
Produced by FastQC (version 0.11.5)