##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR212571_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39208
Sequences flagged as poor quality	0
Sequence length	58
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.88693633952255	39.0	38.0	39.0	35.0	40.0
2	37.063456437461745	39.0	36.0	39.0	33.0	40.0
3	36.6943225872271	38.0	35.0	39.0	31.0	40.0
4	36.50874821465007	38.0	35.0	39.0	31.0	40.0
5	37.27935625382575	38.0	36.0	39.0	33.0	40.0
6	37.78392164864314	39.0	38.0	39.0	35.0	40.0
7	36.93544684758213	38.0	36.0	39.0	33.0	40.0
8	37.81246174250153	39.0	38.0	39.0	35.0	40.0
9	37.79700571311977	39.0	38.0	39.0	35.0	40.0
10	37.037288308508465	38.0	36.0	39.0	33.0	40.0
11	36.803662517853496	38.0	36.0	39.0	33.0	40.0
12	36.51907773923689	38.0	35.0	39.0	31.0	40.0
13	36.15494286880229	38.0	35.0	39.0	30.0	40.0
14	36.48946643542134	38.0	35.0	39.0	31.0	40.0
15	36.43957865741685	38.0	35.0	39.0	31.0	40.0
16	36.06307386247705	38.0	35.0	39.0	30.0	40.0
17	35.29340950826362	38.0	33.0	39.0	28.0	40.0
18	35.36977147520914	38.0	34.0	39.0	28.0	40.0
19	35.01234441950622	38.0	33.0	39.0	27.0	40.0
20	35.051877167924914	38.0	33.0	39.0	28.0	40.0
21	34.49517955519282	38.0	33.0	39.0	26.0	40.0
22	34.45230565190777	38.0	33.0	39.0	26.0	40.0
23	34.28978779840849	37.0	33.0	39.0	26.0	40.0
24	34.34028769638849	37.0	33.0	39.0	26.0	40.0
25	34.092124056315036	37.0	33.0	39.0	26.0	40.0
26	33.82113344215466	37.0	33.0	39.0	24.0	40.0
27	33.51157926953683	36.0	32.0	39.0	23.0	40.0
28	33.30297388288105	36.0	32.0	38.0	23.0	40.0
29	33.04675066312998	36.0	31.0	38.0	23.0	40.0
30	32.93508977759641	36.0	31.0	38.0	23.0	40.0
31	32.00780452968782	35.0	30.0	38.0	19.0	39.0
32	31.70424403183024	35.0	29.0	38.0	18.0	39.0
33	32.23538563558458	36.0	31.0	38.0	20.0	39.0
34	30.86467047541318	35.0	29.0	38.0	14.0	39.0
35	29.874489900020404	34.0	27.0	38.0	10.0	39.0
36	31.300703937971843	35.0	30.0	38.0	12.0	40.0
37	30.97566823097327	35.0	29.0	38.0	8.0	39.0
38	30.55248928790043	35.0	28.0	38.0	6.0	39.0
39	30.6146449704142	35.0	29.0	38.0	2.0	39.0
40	30.23625280554989	35.0	28.0	38.0	2.0	39.0
41	30.878213629871453	35.0	30.0	38.0	2.0	39.0
42	30.61109977555601	35.0	29.0	39.0	2.0	40.0
43	30.41945521322179	35.0	29.0	38.0	2.0	39.0
44	30.522622934095082	35.0	29.0	39.0	2.0	40.0
45	30.058406447663742	35.0	29.0	38.0	2.0	39.0
46	29.328682921852682	35.0	27.0	38.0	2.0	39.0
47	28.77565802897368	35.0	26.0	38.0	2.0	39.0
48	28.939680677412774	35.0	26.0	38.0	2.0	39.0
49	28.611354825545806	35.0	26.0	38.0	2.0	39.0
50	28.374158335033666	34.0	25.0	38.0	2.0	39.0
51	28.192027137318913	35.0	25.0	38.0	2.0	39.0
52	27.862885125484596	34.0	24.0	38.0	2.0	39.0
53	27.56422158743114	34.0	23.0	38.0	2.0	39.0
54	27.458605386655783	34.0	23.0	38.0	2.0	39.0
55	27.126428279942868	34.0	22.0	38.0	2.0	39.0
56	26.931825137726996	35.0	16.0	38.0	2.0	39.0
57	26.58153948173842	34.0	10.0	38.0	2.0	39.0
58	25.831233421750664	33.0	2.0	38.0	2.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-2.9884065945514564
1	2	-8.54860020231861
1	3	-4.246938001968147
1	4	-8.676971042369573
1	5	-5.050910789839101
1	6	-2.401759300639874
1	7	-3.5471856126634336
1	8	-2.3263615595830487
1	9	-2.3057385101898973
1	10	-1.4952683507062048
1	11	-1.0052032532396922
1	12	-7.046859015166248
1	13	-1.1672811863508556
1	14	-0.6460729721697049
1	15	-4.262519453420275
1	16	0.368966259423253
1	17	-0.7465385712375223
1	18	-0.8189661822419367
1	19	-4.404370526031229
1	20	-1.3557352055748453
1	21	-0.6667893001553011
1	22	-2.1681078684987654
1	23	-6.043703176273873
1	24	-7.7894475573581445
1	25	-1.1933222877484795
1	26	-15.0865032411254
1	27	-4.899474583322306
1	28	-15.826943743549045
1	29	-2.702546999059866
1	30	-19.796224421268054
1	31	-10.547959689375944
1	32	-10.286298931658756
1	33	-13.857853632612816
1	34	-15.9234502759419
1	35	-9.727252091634718
1	36	-11.42890041652593
1	37	-9.94307423457317
1	38	-12.336033129782308
1	39	-10.367380690560456
1	40	-10.07362277909781
1	41	-13.554570046767342
1	42	-10.018958406320635
1	43	-9.281354707325853
1	44	-9.166862262704
1	45	-12.738659263018068
1	46	-8.820208339022955
1	47	-8.439578498171784
1	48	-8.447112649475748
1	49	-10.023918741039981
1	50	-9.906816814288298
1	51	-18.07666603567557
1	52	-10.884155684769034
1	53	-7.030100471727618
1	54	-7.044443185721978
1	55	-10.26501151540539
1	56	-10.944809150364357
1	57	-9.12544378754929
1	58	-8.235634640469168
2	1	-0.4884065945514564
2	2	-0.5486002023186103
2	3	-1.2469380019681466
2	4	-2.6769710423695727
2	5	0.4490892101608992
2	6	-0.4017593006398741
2	7	-4.547185612663434
2	8	-0.3263615595830487
2	9	-0.3057385101898973
2	10	0.5047316492937952
2	11	-2.005203253239692
2	12	2.4531409848337518
2	13	-0.6672811863508556
2	14	0.8539270278302951
2	15	2.737480546579725
2	16	2.368966259423253
2	17	-1.2465385712375223
2	18	3.1810338177580633
2	19	2.0956294739687706
2	20	0.6442647944251547
2	21	-3.666789300155301
2	22	-8.168107868498765
2	23	-4.543703176273873
2	24	-0.28944755735814454
2	25	-4.1933222877484795
2	26	0.9134967588746008
2	27	-7.899474583322306
2	28	0.6730562564509555
2	29	-0.7025469990598658
2	30	4.703775578731946
2	31	-8.547959689375944
2	32	-8.286298931658756
2	33	-7.857853632612816
2	34	-0.42345027594189943
2	35	-9.727252091634718
2	36	-3.9289004165259307
2	37	-4.943074234573171
2	38	4.663966870217692
2	39	-3.3673806905604557
2	40	-1.0736227790978106
2	41	1.445429953232658
2	42	-8.018958406320635
2	43	-7.281354707325853
2	44	-0.16686226270400084
2	45	2.261340736981932
2	46	-2.8202083390229546
2	47	1.0604215018282162
2	48	4.5528873505242515
2	49	2.4760812589600185
2	50	0.09318318571170181
2	51	3.9233339643244314
2	52	7.615844315230966
2	53	5.469899528272382
2	54	8.455556814278022
2	55	7.234988484594609
2	56	3.0551908496356432
2	57	5.37455621245071
2	58	0.7643653595308315
3	1	-1.9884065945514564
3	2	-3.0486002023186103
3	3	-4.496938001968147
3	4	-5.676971042369573
3	5	-4.300910789839101
3	6	-1.901759300639874
3	7	-6.547185612663434
3	8	-3.0763615595830487
3	9	-3.0557385101898973
3	10	-4.245268350706205
3	11	-3.505203253239692
3	12	-6.046859015166248
3	13	-6.917281186350856
3	14	-3.396072972169705
3	15	-7.012519453420275
3	16	-1.631033740576747
3	17	1.2534614287624777
3	18	-5.818966182241937
3	19	0.5956294739687706
3	20	-1.8557352055748453
3	21	-2.666789300155301
3	22	2.8318921315012346
3	23	-1.7937031762738727
3	24	-1.2894475573581445
3	25	0.5566777122515205
3	26	-1.8365032411253992
3	27	-12.399474583322306
3	28	-8.826943743549045
3	29	-4.452546999059866
3	30	-0.5462244212680538
3	31	-15.047959689375944
3	32	-8.536298931658756
3	33	-7.107853632612816
3	34	-12.4234502759419
3	35	-13.227252091634718
3	36	0.0710995834740693
3	37	-0.6930742345731709
3	38	0.663966870217692
3	39	-7.117380690560456
3	40	1.4263772209021894
3	41	-2.304570046767342
3	42	-7.768958406320635
3	43	-10.281354707325853
3	44	-7.416862262704001
3	45	-6.738659263018068
3	46	-10.820208339022955
3	47	-4.689578498171784
3	48	-5.4471126494757485
3	49	-4.5239187410399815
3	50	-6.906816814288298
3	51	-7.576666035675569
3	52	-4.884155684769034
3	53	-6.280100471727618
3	54	-2.2944431857219776
3	55	-4.515011515405391
3	56	-5.694809150364357
3	57	-5.37544378754929
3	58	-5.9856346404691685
4	1	-1.9884065945514564
4	2	-3.5486002023186103
4	3	-5.9136046686348145
4	4	-6.010304375702905
4	5	-3.3842441231724365
4	6	-1.5684259673065384
4	7	-4.213852279330101
4	8	-1.493028226249713
4	9	-1.4724051768565616
4	10	-1.661935017372869
4	11	-2.1718699199063565
4	12	-4.046859015166248
4	13	-5.3339478530175235
4	14	-2.9794063055030406
4	15	-3.4291861200869427
4	16	-9.297700407243415
4	17	-8.746538571237522
4	18	-3.8189661822419367
4	19	-5.904370526031229
4	20	-4.022401872241513
4	21	-1.333455966821969
4	22	-1.8347745351654332
4	23	-1.2103698429405405
4	24	-6.622780890691477
4	25	-4.526655621081812
4	26	-5.919836574458731
4	27	-6.732807916655638
4	28	-7.993610410215712
4	29	-7.202546999059866
4	30	-11.462891087934722
4	31	-9.381293022709276
4	32	-4.452965598325424
4	33	-5.357853632612816
4	34	-3.7567836092752316
4	35	-6.3939187583013855
4	36	-5.5955670831925985
4	37	-4.609740901239839
4	38	-8.502699796448976
4	39	-7.200714023893788
4	40	-4.073622779097811
4	41	-2.22123671343401
4	42	-1.3522917396539675
4	43	0.05197862600747882
4	44	-6.666862262704001
4	45	-5.5719925963514
4	46	-4.320208339022955
4	47	-5.106245164838452
4	48	-9.947112649475748
4	49	-10.190585407706648
4	50	-9.740150147621632
4	51	-9.409999369008903
4	52	-9.884155684769034
4	53	-11.196767138394284
4	54	-9.044443185721978
4	55	-9.598344848738725
4	56	-6.611475817031025
4	57	-7.62544378754929
4	58	-7.902301307135835
5	1	-1.2384065945514564
5	2	-7.79860020231861
5	3	-2.9969380019681466
5	4	-6.676971042369573
5	5	-0.3009107898391008
5	6	-1.901759300639874
5	7	-5.297185612663434
5	8	-2.8263615595830487
5	9	-3.0557385101898973
5	10	-0.24526835070620479
5	11	-3.505203253239692
5	12	-3.7968590151662482
5	13	-4.167281186350856
5	14	-1.646072972169705
5	15	-6.762519453420275
5	16	-6.881033740576747
5	17	-10.246538571237522
5	18	-11.568966182241937
5	19	-8.90437052603123
5	20	-6.105735205574845
5	21	-8.666789300155301
5	22	-6.418107868498765
5	23	-5.043703176273873
5	24	-5.7894475573581445
5	25	-8.44332228774848
5	26	-3.836503241125399
5	27	-3.8994745833223057
5	28	-7.3269437435490445
5	29	-2.952546999059866
5	30	-8.296224421268054
5	31	-13.547959689375944
5	32	-17.536298931658756
5	33	-15.107853632612816
5	34	-14.1734502759419
5	35	-12.977252091634718
5	36	-17.42890041652593
5	37	-12.44307423457317
5	38	-16.336033129782308
5	39	-12.117380690560456
5	40	-11.07362277909781
5	41	-13.554570046767342
5	42	-9.518958406320635
5	43	-9.031354707325853
5	44	-11.416862262704
5	45	-9.238659263018068
5	46	-13.570208339022955
5	47	-7.939578498171784
5	48	-8.447112649475748
5	49	-11.523918741039981
5	50	-8.156816814288298
5	51	-15.826666035675569
5	52	-14.634155684769034
5	53	-13.530100471727618
5	54	-14.294443185721978
5	55	-13.26501151540539
5	56	-14.194809150364357
5	57	-13.87544378754929
5	58	-12.985634640469168
6	1	-0.888406594551455
6	2	-1.4486002023186089
6	3	-3.146938001968145
6	4	-2.776971042369574
6	5	-1.9509107898390994
6	6	-1.601759300639877
6	7	-1.5471856126634336
6	8	-0.9263615595830501
6	9	-0.9057385101898987
6	10	-2.8952683507062034
6	11	-2.8052032532396893
6	12	-3.3468590151662454
6	13	-5.567281186350854
6	14	-1.0460729721697035
6	15	-2.262519453420275
6	16	-1.4310337405767442
6	17	-5.746538571237522
6	18	-4.218966182241935
6	19	-2.4043705260312294
6	20	-0.5557352055748481
6	21	-4.0667893001553
6	22	-0.06810786849876393
6	23	-3.3437031762738734
6	24	-3.889447557358146
6	25	0.8066777122515205
6	26	-6.586503241125399
6	27	-9.699474583322306
6	28	-7.626943743549045
6	29	-8.102546999059864
6	30	-5.296224421268054
6	31	-4.847959689375944
6	32	-6.586298931658757
6	33	-7.157853632612817
6	34	-2.323450275941898
6	35	-7.227252091634718
6	36	-7.328900416525929
6	37	-7.043074234573172
6	38	-7.636033129782309
6	39	-6.6673806905604565
6	40	-8.97362277909781
6	41	-4.254570046767341
6	42	-4.518958406320635
6	43	-3.9813547073258526
6	44	-6.4668622627040016
6	45	-3.0386592630180687
6	46	-3.7202083390229532
6	47	-2.5395784981717853
6	48	-0.5471126494757499
6	49	-1.5239187410399815
6	50	-2.406816814288298
6	51	-0.2766660356755679
6	52	0.7158443152309673
6	53	0.4698995282723821
6	54	-2.944443185721976
6	55	1.9349884845946086
6	56	2.455190849635642
6	57	1.5745562124507089
6	58	1.66436535953083
7	1	-0.3634065945514564
7	2	-0.7986002023186103
7	3	-2.1219380019681466
7	4	-2.8019710423695727
7	5	-0.8009107898391008
7	6	-0.2767593006398741
7	7	-3.2971856126634336
7	8	-0.2013615595830487
7	9	-0.6807385101898973
7	10	-2.120268350706205
7	11	-2.005203253239692
7	12	-2.7968590151662482
7	13	-3.9172811863508556
7	14	-2.896072972169705
7	15	-2.512519453420275
7	16	-2.256033740576747
7	17	-1.4965385712375223
7	18	-2.3189661822419367
7	19	-2.1543705260312294
7	20	-7.855735205574845
7	21	-6.416789300155301
7	22	-7.418107868498765
7	23	-9.543703176273873
7	24	-4.1644475573581445
7	25	-4.8183222877484795
7	26	-2.586503241125399
7	27	-1.8994745833223057
7	28	-1.0769437435490445
7	29	-0.9525469990598658
7	30	-4.796224421268054
7	31	-5.7979596893759435
7	32	-4.036298931658756
7	33	-3.232853632612816
7	34	-3.1734502759418994
7	35	-7.352252091634718
7	36	-6.178900416525931
7	37	-4.943074234573171
7	38	-6.961033129782308
7	39	-6.367380690560456
7	40	-8.07362277909781
7	41	-6.429570046767342
7	42	-8.268958406320635
7	43	-8.031354707325853
7	44	-6.166862262704001
7	45	-7.238659263018068
7	46	-7.070208339022955
7	47	-9.814578498171784
7	48	-8.822112649475748
7	49	-8.523918741039981
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119	58	-12.985634640469168
120	1	-2.4884065945514564
120	2	-7.04860020231861
120	3	-3.7469380019681466
120	4	-3.6769710423695727
120	5	-6.550910789839101
120	6	-2.901759300639874
120	7	-11.547185612663434
120	8	-2.3263615595830487
120	9	-3.3057385101898973
120	10	-1.4952683507062048
120	11	-2.005203253239692
120	12	-0.5468590151662482
120	13	-4.167281186350856
120	14	-1.646072972169705
120	15	-9.262519453420275
120	16	-1.631033740576747
120	17	-7.746538571237522
120	18	-5.318966182241937
120	19	-4.404370526031229
120	20	0.14426479442515472
120	21	1.3332106998446989
120	22	-2.1681078684987654
120	23	1.4562968237261273
120	24	-1.7894475573581445
120	25	-5.6933222877484795
120	26	0.9134967588746008
120	27	-2.8994745833223057
120	28	-2.3269437435490445
120	29	-1.7025469990598658
120	30	-4.296224421268054
120	31	-9.047959689375944
120	32	-13.286298931658756
120	33	-8.357853632612816
120	34	-6.923450275941899
120	35	-9.227252091634718
120	36	2.5710995834740693
120	37	-10.94307423457317
120	38	-22.836033129782308
120	39	-12.867380690560456
120	40	-10.07362277909781
120	41	-23.054570046767342
120	42	-13.518958406320635
120	43	-12.781354707325853
120	44	-15.666862262704
120	45	-12.238659263018068
120	46	-10.820208339022955
120	47	-10.439578498171784
120	48	-10.447112649475748
120	49	-20.52391874103998
120	50	-7.906816814288298
120	51	-9.076666035675569
120	52	-22.884155684769034
120	53	-22.530100471727618
120	54	-22.544443185721978
120	55	-22.26501151540539
120	56	-21.944809150364357
120	57	-21.62544378754929
120	58	-20.73563464046917
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35.0
3	3.0
4	3.0
5	11.0
6	21.0
7	43.0
8	43.0
9	80.0
10	121.0
11	146.0
12	198.0
13	220.0
14	219.0
15	268.0
16	295.0
17	317.0
18	374.0
19	341.0
20	383.0
21	505.0
22	555.0
23	569.0
24	633.0
25	602.0
26	758.0
27	886.0
28	1048.0
29	1198.0
30	1426.0
31	1609.0
32	1861.0
33	2171.0
34	2606.0
35	3210.0
36	3915.0
37	5060.0
38	4685.0
39	2787.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	73.80891654764334	17.129157314833705	2.4357274025709037	6.626198734952051
11	40.950826361966946	16.358906345643746	13.759946949602123	28.930320342787187
12	41.29769434809223	9.977555600897777	25.862068965517242	22.862681085492756
13	23.566619057335238	15.777392368904305	30.083146296674144	30.57284227708631
14	16.897061824117525	19.42970822281167	43.934911242603555	19.73831871046725
15	24.58936951642522	13.601815955927362	41.62415833503367	20.184656192613755
16	29.114698905807636	12.49776825566863	37.342821434947844	21.044711403575892
17	27.718832891246688	14.364415425423383	25.091817996327283	32.82493368700265
18	31.326684182332986	14.25911282299824	25.671504731780733	28.742698262888045
19	26.39834723391231	22.126150942433746	31.351544367077306	20.123957456576633
20	35.054070597837175	19.149153234033868	24.55111201795552	21.245664150173436
21	28.351356865945725	21.452254641909814	25.56366047745358	24.63272801469088
22	33.23046317078147	18.71556825137727	25.402978983880843	22.650989593960418
23	38.55080595796776	19.330238726790448	24.44909202203632	17.669863293205466
24	39.28125079705155	16.869436580202514	21.011554059224117	22.83775856352182
25	29.756682309732707	22.79636808814528	21.824627627014895	25.62232197510712
26	29.007575177901906	16.61438008518887	25.972402887239525	28.4056418496697
27	20.765599081281103	15.82493301008039	25.471481434222277	37.93798647441623
28	21.84869662806713	27.592715400703977	21.966025608325257	28.59256236290364
29	32.38872592781533	18.240020405560514	26.603749521744675	22.76750414487948
30	26.99344237197316	16.697711209206194	29.40981347758414	26.899032941236506
31	32.4780002550695	14.735365387067976	28.618798622624663	24.16783573523785
32	41.277008239585726	12.828754368511008	29.481390780847427	16.41284661105584
33	41.691542288557216	11.544839903048858	26.618191095803034	20.14542671259089
34	33.043345153965866	13.577569711967751	36.5538178942266	16.825267239839782
35	26.08107763349236	23.570681429701253	35.43128300635253	14.916957930453862
36	29.00303656638342	31.406772308555976	24.591084232820435	14.99910689224017
37	33.26017608778869	23.01390838330994	24.488962613244862	19.236952915656502
38	24.46979557461144	29.461756373937675	26.810096215195365	19.258351836255517
39	35.523561545923314	22.326032572624698	23.602389339868278	18.548016541583703
40	26.094834626378116	24.696304614128213	24.90046957942017	24.3083911800735
41	26.54535245776122	20.879485478025625	27.795926701036187	24.77923536317697
42	24.592256055539956	22.647336583373743	25.76635442456418	26.994052936522117
43	23.726521028991428	18.60963658636178	27.210085749285422	30.453756635361373
44	25.188248206856063	14.94244071776808	27.78671159098451	32.08259948439135
45	29.943344222131486	14.53399346672111	25.393017558187015	30.129644752960395
46	27.631007989381523	22.79143374939378	25.668121601960337	23.90943665926436
47	24.235924932975873	20.20681731137495	31.533256734329118	24.024001021320057
48	24.98148857398187	19.43827396910507	32.31711987744159	23.26311757947147
49	28.982916677306502	19.149153494548145	30.458874901049516	21.409054927095834
50	21.68671621587107	18.5426404106965	31.72170714887748	28.04893622455495
51	22.149313179798806	15.763672573150181	34.38186181892458	27.705152428126432
52	18.811754793576224	17.65516889217964	34.77161896494498	28.761457349299157
53	16.93348653134176	17.857781182177966	40.653644835950466	24.55508745052981
54	20.92262893049631	18.838284502797006	34.76206288794094	25.477023678765743
55	20.909555442003068	15.838017373530915	33.99846704138989	29.253960143076135
56	20.807032787304184	19.375942347499425	25.62674094707521	34.19028391812118
57	24.070525105404368	19.16954133128913	24.821770793407435	31.93816276989907
58	21.701801456496742	27.412801839785356	27.292704740002556	23.592691963715346
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.5
9	16.0
10	29.0
11	28.5
12	28.0
13	40.0
14	52.0
15	110.5
16	235.0
17	301.0
18	245.5
19	190.0
20	232.5
21	275.0
22	335.0
23	447.5
24	500.0
25	578.0
26	656.0
27	690.0
28	688.5
29	653.0
30	574.0
31	495.0
32	530.5
33	566.0
34	499.0
35	389.0
36	346.0
37	319.5
38	293.0
39	317.5
40	342.0
41	376.5
42	450.5
43	490.0
44	881.5
45	1273.0
46	1054.5
47	960.0
48	1084.0
49	1048.5
50	1013.0
51	1268.5
52	1524.0
53	1521.0
54	1476.0
55	1434.0
56	1908.5
57	2383.0
58	3030.0
59	3958.0
60	4239.0
61	4448.5
62	4658.0
63	3851.5
64	3045.0
65	2569.5
66	1674.0
67	1254.0
68	981.0
69	708.0
70	588.0
71	468.0
72	367.5
73	208.0
74	149.0
75	121.0
76	93.0
77	74.0
78	49.0
79	43.0
80	30.0
81	17.0
82	18.0
83	19.0
84	11.5
85	3.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.002550499897980004
17	0.0
18	0.012752499489900022
19	0.002550499897980004
20	0.0
21	0.0
22	0.0
23	0.0
24	0.002550499897980004
25	0.0
26	0.002550499897980004
27	0.05866149765354009
28	0.005100999795960008
29	0.007651499693940012
30	0.04335849826566007
31	0.007651499693940012
32	0.01785349928586003
33	0.03315649867374005
34	0.028055498877780045
35	0.028055498877780045
36	0.04845949806162008
37	0.05866149765354009
38	0.0637624974495001
39	0.08671699653132015
40	0.0612119975515201
41	0.0663129973474801
42	0.07396449704142012
43	0.0612119975515201
44	0.07906549683738012
45	0.0612119975515201
46	0.07906549683738012
47	0.10967149561314019
48	0.10967149561314019
49	0.11987349520506019
50	0.14027749438890022
51	0.10712099571516018
52	0.10457049581718016
53	0.10967149561314019
54	0.15047949398082025
55	0.1734339930626403
56	0.19638849214446033
57	0.1861864925525403
58	0.1861864925525403
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
58	39208.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.600081615996736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.70617720772519	24.4643950214242
2	11.1749963143152	7.733115690675373
3	5.307386112339673	5.509079779636808
4	3.0370042753943682	4.203223831871047
5	2.1745540321391714	3.7619873495205063
6	1.4153029632905794	2.9381758824729647
7	1.0319917440660475	2.499489900020404
8	0.700280112044818	1.938379922464803
9	0.5897095680377414	1.836359926545603
>10	3.464543712221731	22.992756580289736
>50	0.22851245761462477	5.376453784941849
>100	0.1400560224089636	8.521220159151193
>500	0.022114108801415303	5.394307284227708
>1k	0.007371369600471768	2.8310548867578045
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	1110	2.8310548867578045	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	844	2.1526219138951235	No Hit
TCGTAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	677	1.7266884309324628	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	594	1.5149969394001226	No Hit
TCGTAAGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	357	0.9105284635788614	No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	329	0.8391144664354213	No Hit
TCGTAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	287	0.7319934707202612	No Hit
TCGTAAGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTG	233	0.5942664762293409	No Hit
TCGTAAGGGGGGTTAGAGGGGGAAGGGGTTGTGTGTTTTCTTTTTTTGAGACAAGGCT	219	0.5585594776576209	No Hit
TCGTAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA	185	0.4718424811263008	No Hit
TCGTAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	181	0.46164048153438075	No Hit
TCGTAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	175	0.4463374821465007	No Hit
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT	161	0.41063048357478066	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA	135	0.3443174862273005	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	134	0.34176698632932057	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA	129	0.32901448683942053	No Hit
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT	127	0.3239134870434605	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG	126	0.3213629871454805	No Hit
TCGTAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	125	0.3188124872475005	No Hit
TCGTAAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC	122	0.3111609875535605	No Hit
TCGTAAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC	109	0.27800448887982043	No Hit
TCGTAAGGGGTGGTATCAACGCAGAATACAGTGCTTCGTAAGGGGGGGCTGGTGAGAT	106	0.2703529891858804	No Hit
TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	101	0.2576004896959804	No Hit
TCGTAAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA	99	0.2524994899000204	No Hit
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT	97	0.2473984901040604	No Hit
TCGTAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	92	0.23464599061416036	No Hit
TCGTAAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG	89	0.2269944909202204	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA	86	0.21934299122628037	No Hit
TCGTAAGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC	85	0.21679249132830034	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCGGACTGCTCTTCCGA	84	0.21424199143032036	No Hit
TCGTAAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC	76	0.19383799224648032	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA	76	0.19383799224648032	No Hit
TCGTAAGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGT	76	0.19383799224648032	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCG	69	0.17598449296062027	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG	69	0.17598449296062027	No Hit
TCGTAAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT	68	0.1734339930626403	No Hit
TCGTAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA	67	0.17088349316466028	No Hit
TCGTAAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA	67	0.17088349316466028	No Hit
TCGTAAGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCCG	62	0.15813099367476025	No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	61	0.15558049377678024	No Hit
TCGTAAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC	61	0.15558049377678024	No Hit
TCGTAAGGGATAGAACAAGAGAGAATCATCTCTAACTTATTTTATGAGGCCAGAATCA	60	0.15302999387880023	No Hit
TCGTAAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	60	0.15302999387880023	No Hit
TCGTAAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA	59	0.15047949398082025	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA	59	0.15047949398082025	No Hit
TCGTAAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGTCC	58	0.14792899408284024	No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT	57	0.14537849418486024	No Hit
TCGTAAGGGAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTG	55	0.14027749438890022	No Hit
TCGTAAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGGCC	53	0.1351764945929402	No Hit
TCGTAAGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGGTC	53	0.1351764945929402	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCGA	53	0.1351764945929402	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCG	53	0.1351764945929402	No Hit
TCGTAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	53	0.1351764945929402	No Hit
TCGTAAGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC	51	0.13007549479698022	No Hit
TCGTAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	50	0.1275249948990002	No Hit
TCGTAAGGGAGCTAATTGCCAAATTACAGATGAGTTACTCCCACAGTTTATTTATTTT	48	0.1224239951030402	No Hit
TCGTAAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGCCC	48	0.1224239951030402	No Hit
TCGTAAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG	47	0.11987349520506019	No Hit
TCGTAAGGGGGGGCTGATGAGATGGCTCAACGGTTAAGAGCACTGATTGCTCTTCTGA	46	0.11732299530708018	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCGA	46	0.11732299530708018	No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC	46	0.11732299530708018	No Hit
TCGTAAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA	46	0.11732299530708018	No Hit
TCGTAAGGGATATGGTCTGTGACTATAGCTGACGTCTTCTGCAGAGGCAGTATGTTCA	46	0.11732299530708018	No Hit
TCGTAAGGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGTCA	45	0.11477249540910019	No Hit
TCGTAAGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC	45	0.11477249540910019	No Hit
TCGTAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45	0.11477249540910019	No Hit
TCGTAAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC	45	0.11477249540910019	No Hit
TCGTAAGGGACCATAGCCAGTGCCAGCACGGCCTCCAGCATTCCCAGGCCTGCGCACT	45	0.11477249540910019	No Hit
TCGTAAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA	45	0.11477249540910019	No Hit
TCGTAAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA	44	0.11222199551112018	No Hit
TCGTAAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	44	0.11222199551112018	No Hit
TCGTAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA	44	0.11222199551112018	No Hit
TCGTAAGGGGAAGTGGAGCTAGCCATAAACCTCAAGGCTTGTTTCCCAGAGATCCACT	44	0.11222199551112018	No Hit
TCGTAAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC	44	0.11222199551112018	No Hit
TCGTAAGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	44	0.11222199551112018	No Hit
TCGTAAGGGGCCTTTTTTTCCCTCAGCGCTGGCCGCGGCCTCCCGGCGCTCTCGTCCT	43	0.10967149561314019	No Hit
TCGTAAGGGACCCCATTGTTTCAAAGCTCCAGTTAAAGAGGGCCACATGTACTGCTCC	43	0.10967149561314019	No Hit
TCGTAAGGGGCTCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC	43	0.10967149561314019	No Hit
TCGTAAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT	43	0.10967149561314019	No Hit
TCGTAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA	42	0.10712099571516018	No Hit
TCGTAAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCCC	42	0.10712099571516018	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCCGA	41	0.10457049581718016	No Hit
TCGTAAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG	41	0.10457049581718016	No Hit
TCGTAAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA	41	0.10457049581718016	No Hit
TCGTAAGGGGGTGGCGCGGAGAACTCTGCAAAACAAGAGGCTGGGGATTGCGTTAGCG	41	0.10457049581718016	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCA	41	0.10457049581718016	No Hit
TCGTAAGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAATTCTCAG	40	0.10201999591920018	No Hit
TCGTAAGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGG	40	0.10201999591920018	No Hit
TCGTAAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA	40	0.10201999591920018	No Hit
TCGTAAGGGGCTTTCGACCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATGACGAAC	40	0.10201999591920018	No Hit
TCGTAAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA	40	0.10201999591920018	No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTGCTCTTCC	40	0.10201999591920018	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.002550499897980004	0.0	0.0	0.0	0.0
11	0.005100999795960008	0.0	0.0	0.0	0.0
12	0.005100999795960008	0.0	0.0	0.0	0.0
13	0.005100999795960008	0.0	0.0	0.0	0.0
14	0.010201999591920016	0.0	0.0	0.0	0.0
15	0.010201999591920016	0.0	0.0	0.0	0.0
16	0.010201999591920016	0.0	0.0	0.0	0.0
17	0.010201999591920016	0.0	0.0	0.0	0.0
18	0.010201999591920016	0.0	0.0	0.0	0.0
19	0.010201999591920016	0.0	0.0	0.0	0.0
20	0.01275249948990002	0.0	0.0	0.0	0.0
21	0.01275249948990002	0.0	0.0	0.0	0.0
22	0.015302999387880024	0.0	0.0	0.0	0.0
23	0.015302999387880024	0.0	0.0	0.0	0.0
24	0.015302999387880024	0.0	0.0	0.0	0.0
25	0.015302999387880024	0.0	0.0	0.0	0.0
26	0.015302999387880024	0.0	0.0	0.0	0.0
27	0.01785349928586003	0.0	0.0	0.0	0.0
28	0.022954499081820037	0.0	0.0	0.0	0.0
29	0.022954499081820037	0.0	0.0	0.0	0.0
30	0.022954499081820037	0.0	0.0	0.0	0.0
31	0.022954499081820037	0.0	0.0	0.0	0.0
32	0.022954499081820037	0.0	0.0	0.0	0.0
33	0.022954499081820037	0.0	0.0	0.0	0.0
34	0.022954499081820037	0.0	0.0	0.0	0.0
35	0.02550499897980004	0.0	0.0	0.0	0.0
36	0.02550499897980004	0.0	0.0	0.0	0.0
37	0.03060599877576005	0.0	0.0	0.0	0.0
38	0.03060599877576005	0.0	0.0	0.0	0.0
39	0.03060599877576005	0.0	0.0	0.0	0.0
40	0.033156498673740056	0.0	0.0	0.0	0.0
41	0.03570699857172006	0.0	0.0	0.0	0.0
42	0.03570699857172006	0.0	0.0	0.0	0.0
43	0.03570699857172006	0.0	0.0	0.0	0.0
44	0.03570699857172006	0.0	0.0	0.0	0.0
45	0.03570699857172006	0.0	0.0	0.0	0.0
46	0.03570699857172006	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCCCA	25	1.5884361E-5	52.203846	52
ATATGGG	15	0.007058178	52.203846	52
AAAACCA	20	3.3413235E-4	52.203846	52
CCAACTG	20	3.3625777E-4	52.137005	43
GTGTTGT	15	0.0070940964	52.137005	44
CAGCATC	15	0.0070940964	52.137005	43
CCTATCG	25	1.6005655E-5	52.137005	47
CAGCACT	15	0.0070940964	52.137005	41
ACGTTTC	15	0.0070940964	52.137005	40
GCAGGTG	15	0.0070940964	52.137005	40
CAACTGC	20	3.3625777E-4	52.137005	44
TATCGGG	40	1.7735147E-9	52.137005	49
CGTAGCG	25	1.6005655E-5	52.137005	41
AGGTGTT	15	0.0070940964	52.137005	42
TCAGCAT	15	0.0070940964	52.137005	42
CCGTAGC	25	1.6005655E-5	52.137005	40
CCCAACT	20	3.3625777E-4	52.137005	42
CACCCAA	20	3.3625777E-4	52.137005	40
ATCGGGG	40	1.7735147E-9	52.137005	50
AGGCTAC	25	1.6005655E-5	52.137005	51
>>END_MODULE