FastQCFastQC Report
Tue 31 May 2016
SRR212570_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212570_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40478
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT10842.6779979247986563No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA7851.9393250654676615No Hit
TCGTAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC6171.5242847966796778No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5391.331587529028114No Hit
TCGTAAGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3230.7979643263007066No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3090.7633776372350413No Hit
TCGTAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG2720.6719699589900686No Hit
TCGTAAGGGGGGTTAGAGGGGGAAGGGGTTGTGTGTTTTCTTTTTTTGAGACAAGGCT2210.5459755916794308No Hit
TCGTAAGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTG2080.5138593804041701No Hit
TCGTAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1830.4520974356440536No Hit
TCGTAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1700.4199812243687929No Hit
TCGTAAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC1570.3878650130935323No Hit
TCGTAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1490.36810119077029496No Hit
TCGTAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1400.345866890656653No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1390.34339641286624834No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG1260.31128020159098774No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1220.30139829042936905No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1200.2964573348485597No Hit
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1140.28163446810613174No Hit
TCGTAAGGGGTGGTATCAACGCAGAATACAGTGCTTCGTAAGGGGGGGCTGGTGAGAT1040.25692969020208506No Hit
TCGTAAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC1020.25198873462127575No Hit
TCGTAAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGTCC1010.24951825683087106No Hit
TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.24951825683087106No Hit
TCGTAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1000.24704777904046646No Hit
TCGTAAGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC950.2346953900884431No Hit
TCGTAAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA920.2272839567172291No Hit
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT910.22481347892682446No Hit
TCGTAAGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGT880.21740204555561046No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCG870.21493156776520578No Hit
TCGTAAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC840.20752013439399178No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA820.2025791788131825No Hit
TCGTAAGGGATAGAACAAGAGAGAATCATCTCTAACTTATTTTATGAGGCCAGAATCA780.1926972676515638No Hit
TCGTAAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA780.1926972676515638No Hit
TCGTAAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT730.1803448786995405No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC680.16799248974751715No Hit
TCGTAAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA660.16305153416670784No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCGGACTGCTCTTCCGA640.1581105785858985No Hit
TCGTAAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC620.15316962300508918No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA610.15069914521468453No Hit
TCGTAAGGGACCATAGCCAGTGCCAGCACGGCCTCCAGCATTCCCAGGCCTGCGCACT610.15069914521468453No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT610.15069914521468453No Hit
TCGTAAGGGGTTCTCCTCTCCCCGTCATGGCTTGTGCCCGTCCCCCCATATCGGGGTA600.14822866742427984No Hit
TCGTAAGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA600.14822866742427984No Hit
TCGTAAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG590.14575818963387518No Hit
TCGTAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA570.14081723405306587No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG560.13834675626266119No Hit
TCGTAAGGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGTCA540.13340580068185187No Hit
TCGTAAGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGG530.13093532289144721No Hit
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT520.12846484510104253No Hit
TCGTAAGGGATATGGTCTGTGACTATAGCTGACGTCTTCTGCAGAGGCAGTATGTTCA520.12846484510104253No Hit
TCGTAAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA510.12599436731063787No Hit
TCGTAAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA500.12352388952023323No Hit
TCGTAAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA500.12352388952023323No Hit
TCGTAAGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAATTCTCAG480.11858293393942389No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCGA480.11858293393942389No Hit
TCGTAAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA480.11858293393942389No Hit
TCGTAAGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGGTC480.11858293393942389No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCGA480.11858293393942389No Hit
TCGTAAGGGAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTG470.11611245614901922No Hit
TCGTAAGGGGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA470.11611245614901922No Hit
TCGTAAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT470.11611245614901922No Hit
TCGTAAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA460.11364197835861455No Hit
TCGTAAGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG460.11364197835861455No Hit
TCGTAAGGGGGGGCTGATGAGATGGCTCAACGGTTAAGAGCACTGATTGCTCTTCTGA450.1111715005682099No Hit
TCGTAAGGGGGCACGTGCTGCTCCGGCTCCCGCGTGGCGTTCTCAGCATCCAGTCTTC450.1111715005682099No Hit
TCGTAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA440.10870102277780523No Hit
TCGTAAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT440.10870102277780523No Hit
TCGTAAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC440.10870102277780523No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCG440.10870102277780523No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCA440.10870102277780523No Hit
TCGTAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA430.10623054498740056No Hit
TCGTAAGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC420.10376006719699589No Hit
TCGTAAGGGGCTCTTTCGGCCTTGCTTGCCGGCAGACTCGCCGCCATGGGCCGTGTGA420.10376006719699589No Hit
TCGTAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.10376006719699589No Hit
TCGTAAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTGTGCCCGCCC420.10376006719699589No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCGA420.10376006719699589No Hit
TCGTAAGGGGCCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAGACCC410.10128958940659125No Hit
TCGTAAGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCCG410.10128958940659125No Hit
TCGTAAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA410.10128958940659125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTCT353.5632183E-852.353318
GAAGCAT251.562549E-552.353319
AGGGCTT251.562549E-552.353316
AGGGTGT251.562549E-552.353316
AAGGGAA1750.052.353315
AGGGACT353.5632183E-852.353316
AGGGAAC503.6379788E-1252.353316
AAGCATG251.562549E-552.3533110
AAGCAGT458.185452E-1152.3533110
AGGGGGC1200.052.3533066
AGGGGAT150.006980201752.3533066
GGATGCC150.006980201752.3533068
GGGTCGA150.006980201752.3533068
GATGCCA150.006980201752.3533069
GAACGCA150.006980201752.3533069
AGGGCAT401.711669E-952.3533066
GGACAGT203.2955722E-452.3533068
AAGGGTC307.445324E-752.3533065
AAGGGAC1200.052.3533065
GCGTGAA150.006980201752.35330610
Produced by FastQC (version 0.11.5)