FastQCFastQC Report
Tue 31 May 2016
SRR212568_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212568_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59836
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC20843.4828531318938434No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC14532.428304031018116No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC11881.985426833344475No Hit
TCGTAAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA8591.4355906143458788No Hit
TCGTAAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC7991.3353165318537337No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT5740.959288722508189No Hit
TCGTAAGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCG4300.7186309245270406No Hit
TCGTAAGGGGGGTTAGAGGGGGAAGGGGTTGTGTGTTTTCTTTTTTTGAGACAAGG4250.7102747509860284No Hit
TCGTAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4100.6852062303629921No Hit
TCGTAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA4090.6835349956547897No Hit
TCGTAAGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT3560.5949595561200615No Hit
TCGTAAGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3130.5230964636673574No Hit
TCGTAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2750.4595895447556655No Hit
TCGTAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC2680.4478909017982486No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC2110.3526305234307106No Hit
TCGTAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.33926064576509124No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC1960.32756200280767434No Hit
TCGTAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1940.32421953339126947No Hit
TCGTAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1890.3158633598502574No Hit
TCGTAAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC1740.2907948392272211No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTC1560.2607126144795775No Hit
TCGTAAGGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT1540.2573701450631727No Hit
TCGTAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA1540.2573701450631727No Hit
TCGTAAGGGGTGGTATCAACGCAGAATACAGTGCTTCGTAAGGGGGGGCTGGTGAG1390.23230162444013636No Hit
TCGTAAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC1370.22895915502373154No Hit
TCGTAAGGGATATGGTCTGTGACTATAGCTGACGTCTTCTGCAGAGGCAGTATGTT1330.22227421619092186No Hit
TCGTAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1230.20556186910889762No Hit
TCGTAAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG1190.19887693027608797No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCGGACTGCTCTTCC1170.19553446085968312No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT1140.19052075673507587No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTT1130.18884952202687344No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTC1110.18550705261046863No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC1090.18216458319406376No Hit
TCGTAAGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC1050.1754796443612541No Hit
TCGTAAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACA1020.17046594023664682No Hit
TCGTAAGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCC1020.17046594023664682No Hit
TCGTAAGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAG1020.17046594023664682No Hit
TCGTAAGGGGCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1010.16879470552844442No Hit
TCGTAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC990.16545223611203957No Hit
TCGTAAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC990.16545223611203957No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCC980.16378100140383717No Hit
TCGTAAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC910.15208235844642023No Hit
TCGTAAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTTTGCCCGC860.14372618490540812No Hit
TCGTAAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAAC850.14205495019720568No Hit
TCGTAAGGGAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTTTTGCCCCC840.14038371548900327No Hit
TCGTAAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT830.13871248078080087No Hit
TCGTAAGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA830.13871248078080087No Hit
TCGTAAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCT830.13871248078080087No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC810.13537001136439603No Hit
TCGTAAGGGGCCCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAGA800.1336987766561936No Hit
TCGTAAGGGATTGCAGAGCTTGGGTTTTGACAAATCAACAAGAGATTCCCATCAGA790.13202754194799118No Hit
TCGTAAGGGAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTG790.13202754194799118No Hit
TCGTAAGGGCTAGATTCTAAGGATCATGTCTGCGAGTCAGGATTCTCGATCCAGAG750.1253426031151815No Hit
TCGTAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA750.1253426031151815No Hit
TCGTAAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC750.1253426031151815No Hit
TCGTAAGGGGTTCTCCTCTCCCCGTCATGGCTTTTGCCCGGCCCCCCCTATCGGGG740.12367136840697908No Hit
TCGTAAGGGAGCTAATTGCCAAATTACAGATGAGTTACTCCCACAGTTTATTTATT730.12200013369877666No Hit
TCGTAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT730.12200013369877666No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTT720.12032889899057422No Hit
TCGTAAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT710.11865766428237183No Hit
TCGTAAGGGACTTTTGTATTTGCCAGGCATAGCCTCACATGAGACAGCTATATCAG700.1169864295741694No Hit
TCGTAAGGGGGGGCTGATGAGATGGCTCAACGGTTAAGAGCACTGATTGCTCTTCT700.1169864295741694No Hit
TCGTAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.11531519486596697No Hit
TCGTAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG680.11364396015776455No Hit
TCGTAAGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCC670.11197272544956215No Hit
TCGTAAGGGGGCACGTGCTGCTCCGGCTCCCGCGTGGCGTTCTCAGCATCCAGTCT660.11030149074135973No Hit
TCGTAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC660.11030149074135973No Hit
TCGTAAGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC650.10863025603315729No Hit
TCGTAAGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGG650.10863025603315729No Hit
TCGTAAGGGGTTCTCCTCTCCCCGTCATGGCTTTTGCCCGTCCCCCCCTATCGGGG650.10863025603315729No Hit
TCGTAAGGGGCCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAGAC640.10695902132495487No Hit
TCGTAAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATC640.10695902132495487No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCTACTGTTCTCCC630.10528778661675246No Hit
TCGTAAGGGACCCCATTGTTTCAAAGCTCCAGTTAAAGAGGGCCACATGTACTGCT630.10528778661675246No Hit
TCGTAAGGGGGGAATTCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCGCGAT630.10528778661675246No Hit
TCGTAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.10361655190855004No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC610.10194531720034762No Hit
TCGTAAGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC600.10027408249214519No Hit
TCGTAAGGGACCATAGCCAGTGCCAGCACGGCCTCCAGCATTCCCAGGCCTGCGCA600.10027408249214519No Hit
TCGTAAGGGGCTTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGA600.10027408249214519No Hit
TCGTAAGGGGAAGCTCACTGGCATGGCCTTTCCGTGTTCCTACCCCCAATGTGTCC600.10027408249214519No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGTT150.00828009450.17003650
TTATGAG150.00828009450.17003642
TTGTCGG150.00828009450.17003637
ACCGGAC301.0071708E-650.17003641
AAACTGG150.00828009450.17003639
CAGTCTA150.00828009450.17003649
GAGCGCA150.00828009450.17003643
CCGGACT301.0071708E-650.17003642
GCCTTCC150.00828009450.17003649
CACATGT150.00828009450.17003644
GCGACTG150.00828009450.17003650
TGCGCCA150.00828009450.17003645
ACGCACC204.082718E-450.17003650
TACGGAG204.082718E-450.17003636
CACCGGA301.0071708E-650.17003640
TATCGGG402.5320332E-950.17003649
AAATACG150.00828009450.17003638
CCAGAAT150.00828009450.17003650
ACCGTAG301.0071708E-650.17003639
GCGCGAT204.082718E-450.17003650
Produced by FastQC (version 0.11.5)