FastQCFastQC Report
Tue 31 May 2016
SRR212567_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212567_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19505
Sequences flagged as poor quality0
Sequence length58
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGATGTGAGTTAAAGTCAGTCAGTGCTTCTGTCTGCTTGAGCTACTTACCT3751.9225839528326072No Hit
GCCCTCGGGATCACAGGCAGTCCTCAGCTGCCTGTGTGGGTGCTGGGAACTGAACCTG3371.7277621122789029No Hit
GCCCTCGGGAGCAGGACCTAGGACTCCTCTCCTACCTCTTTTGAAAGGTTCTTTTCGT1860.9536016406049732No Hit
GCCCTCGGGAGAACATACACATAGAACATACACACACACAGAACATACAGAACATACA1760.9023327351961036No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC1580.8100487054601384No Hit
GCCCTCGGGATGTGAATTTTTAAGCTGCGAATCTGATGGCCTTAATTTCCTTTTTGAC1330.6818764419379646No Hit
GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1210.6203537554473213No Hit
GCCCTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC1190.6100999743655473No Hit
GCCCTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG1040.533196616252243No Hit
GCCCTCGGGATCACAGGCAGTCCTCAGCTGCCTGTGTGGGGGCTGGGAACTGAACCTG980.5024352730069213No Hit
GCCCTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA930.4768008203024866No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC890.4562932581389388No Hit
GCCCTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA860.4409125865162779No Hit
GCCCTCGGGAAATTGAACCAGCTGCAGTCAGTTGCCGTGAAATTAAGAGAAGCAGAAA860.4409125865162779No Hit
GCCCTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC860.4409125865162779No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCCC790.4050243527300692No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC740.3793899000256345No Hit
GCCCTCGGGAGTATTGCTGGGTTTAATTTTTCCTACTATTATTGATTCTTGATATTCA690.3537554473211997No Hit
GCCCTCGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC640.3281209946167649No Hit
GCCCTCGGGAGCAAGACATGTATTCTTAATATGTAATTTGTGATATTTGAAAATAATG630.322994104075878No Hit
GCCCTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC500.25634452704434757No Hit
GCCCTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.24096385542168677No Hit
GCCCTCGGGAGACCAGCTGTGTGGCCCTTTTGGTTTTGGAGACCCCATACCTACAGCT470.24096385542168677No Hit
GCCCTCGGGAGCAGGGTCAAGCTACATAGAGATAACTAAGACCTCCTATAGAAAACAA440.2255831837990259No Hit
GCCCTCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT410.21020251217636504No Hit
GCCCTCGGGATGGTTGCTTCTGAATTTTTGCATTTATGAAAGCTGTCTCCATTGCTAT400.20507562163547807No Hit
GCCCTCGGGAGCCTTTATGATGTGAGCTCTCAGCTGTTCACACTGCCATGCCTCCGTT370.18969495001281725No Hit
GCCCTCGGGGGAATTCCTTCGGCAACATTAACTGGCTGATGCTGAAAAACATCGAACT360.18456805947193028No Hit
GCCCTCGGGAAGAGAGACTTCATACAAGTCCTGCCTTTCAAAGTGTCACTTGGTTGCA350.17944116893104334No Hit
GCCCTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT350.17944116893104334No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCTCC340.17431427839015637No Hit
GCCCTCGGGATTTGAAGGTGGCTCCTGCCACCTGCTAATAGCAGTTCAAACTAAATTT330.16918738784926943No Hit
GCCCTCGGGATGTAAGGACATATAAGTTTGTTCTAGAAAGAAGTAGAATTTCTGTATC320.16406049730838246No Hit
GCCCTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.16406049730838246No Hit
GCCCTCGGGACTGGGTTTTCTGTTTGTTTTTAAGAAACAAAACCACAATTGCTCTGAG310.15893360676749552No Hit
GCCCTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.15380671622660858No Hit
GCCCTCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG290.1486798256857216No Hit
GCCCTCGGGAGCTTCAGGAATCAAAATGTGAAAGATTGAAATATTAATTTATGTTGCT290.1486798256857216No Hit
GCCCTCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA280.14355293514483466No Hit
GCCCTCGGGATGTAGCGTTTGCCACAGGTCAGACTGGTTCATTTCCTAGCTAGAAGCC280.14355293514483466No Hit
GCCCTCGGGGATTTTCACAGCATAGCTCCAGGCAGCTGACACTCGCAGTTATTGCAGG270.1384260446039477No Hit
GCCCTCGGGGAATATTCAGTATGGCCTCCTCTTACGTTGGTGAGTCGTCTTGCCAACA270.1384260446039477No Hit
GCCCTCGGGGATAAGATGGCGGCGGACACTGGGAGCTGGTGCGTGTACGCCGTGCTGC270.1384260446039477No Hit
GCCCTCGGGGAGCATCGAGGTTCCTGAGCATGAGTTAAGGGACATCCACAGAAAGCCA260.13329915406306075No Hit
GCCCTCGGGAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTG260.13329915406306075No Hit
GCCCTCGGGAGGGATGGTGATTCAGCTAGAAGTTCTTTTATTGAGAATAGCCTTTGCT260.13329915406306075No Hit
GCCCTCGGGAGCTCGAGCGGCCTTCTCAGTCGAACCGTTCACGTTGCGAGCTGCTGGC260.13329915406306075No Hit
GCCCTCGGGAACCACACTGCTACCTCCCAGATCAAGGGAGTACTTTTTCTCCGTAAAA260.13329915406306075No Hit
GCCCTCGGGAGTTCTGCCCAGCCCTGACCTGTCCTTAGATAACAGAAACATCTTTGAT250.12817226352217378No Hit
GCCCTCGGGAGCCTGACTAACTCCAAATGATGATGCTCACATGGAAAACGATGAACCA250.12817226352217378No Hit
GCCCTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.12817226352217378No Hit
GCCCTCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA230.1179184824403999No Hit
GCCCTCGGGATAATATCTCATTTAAAGGAGGTTCTTTATGAACTTGGTGTCCATTGTC230.1179184824403999No Hit
GCCCTCGGGATTGTAGAATATCTTAAAAAGCTTAGTCTGACCAGTCTGATATGCATGG220.11279159189951295No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCCC220.11279159189951295No Hit
GCCCTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.10766470135862599No Hit
GCCCTCGGGGAGTTCACTTCCGGCCGCCTGCGGATCCAGATGTGACTCTCAGCTCTTT210.10766470135862599No Hit
GCCCTCGGGAAATTAGGAGTCAAGGAATTATAGCTCTGAGTTACCATGACTGGGAGGA210.10766470135862599No Hit
GCCCTCGGGTCTCTCTAGGCGTCGCTCTCTTGGTGTGCTTGTTCTTGACCCGGTCAAT210.10766470135862599No Hit
GCCCTCGGGATACAAAACTGTGACAGAGTTATCTAGTTATGTTTGTCTTTGTGAATAG200.10253781081773904No Hit
GCCCTCGGGATTGTCTGTTCTATTTATTTTTCCAGTGAAAAGTATTTTGATAGAGCTT200.10253781081773904No Hit
GCCCTCGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTTTTCTTCCGCCCC200.10253781081773904No Hit
GCCCTCGGGAAGATATTACCATGAGATTTGGGGATAAGAAATGGAAAGTTATTGCACA200.10253781081773904No Hit
GCCCTCGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCG200.10253781081773904No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCA251.5758473E-552.1079743
TGGTTGC150.007056328552.1079751
GAGCGCA150.007056328552.1079743
TACAGAA150.007056328552.1079746
ACTTGGT150.007056328552.1079748
ACTGAAC353.5663106E-852.1079749
GAAAATA203.329723E-452.1079749
GAAGATG150.007056328552.1079752
CTTGGTT150.007056328552.1079749
CTGGTGG150.007056328552.1079749
CTGAACC353.5663106E-852.1079750
AGGCCGC401.7025741E-952.1079749
TATTTTG150.007056328552.1079743
CACTTGG150.007056328552.1079747
CGCAGGC401.7025741E-952.1079746
TTGATAT150.007056328552.1079749
GCGCAGG401.7025741E-952.1079745
AGGCACC150.007056328552.1079750
GCCGCTC307.485178E-752.1079751
GCACCTC150.007056328552.1079752
Produced by FastQC (version 0.11.5)