FastQCFastQC Report
Tue 31 May 2016
SRR212566_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212566_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20022
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGATGTGAGTTAAAGTCAGTCAGTGCTTCTGTCTGCTTGAGCTACTTACCT3651.822994705823594No Hit
GCCCTCGGGATCACAGGCAGTCCTCAGCTGCCTGTGTGGGTGCTGGGAACTGAACCTG3421.7081210668264908No Hit
GCCCTCGGGAGAACATACACATAGAACATACACACACACAGAACATACAGAACATACA2000.9989012086704625No Hit
GCCCTCGGGAGCAGGACCTAGGACTCCTCTCCTACCTCTTTTGAAAGGTTCTTTTCGT1750.8740385575866547No Hit
GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1660.8290880031964839No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC1430.7142143641993807No Hit
GCCCTCGGGATGTGAATTTTTAAGCTGCGAATCTGATGGCCTTAATTTCCTTTTTGAC1220.6093297372889822No Hit
GCCCTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC1210.6043352312456298No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC1050.5244231345519929No Hit
GCCCTCGGGATCACAGGCAGTCCTCAGCTGCCTGTGTGGGGGCTGGGAACTGAACCTG1050.5244231345519929No Hit
GCCCTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG1050.5244231345519929No Hit
GCCCTCGGGAAATTGAACCAGCTGCAGTCAGTTGCCGTGAAATTAAGAGAAGCAGAAA890.44451103785835583No Hit
GCCCTCGGGAGACCAGCTGTGTGGCCCTTTTGGTTTTGGAGACCCCATACCTACAGCT840.4195385076415942No Hit
GCCCTCGGGAGTATTGCTGGGTTTAATTTTTCCTACTATTATTGATTCTTGATATTCA830.41454400159824195No Hit
GCCCTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC810.40455498951153734No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCCC770.3845769653381281No Hit
GCCCTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.37958245929477574No Hit
GCCCTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA640.319648386774548No Hit
GCCCTCGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC640.319648386774548No Hit
GCCCTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC630.31465388073119566No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC610.3046648686444911No Hit
GCCCTCGGGAGCAAGACATGTATTCTTAATATGTAATTTGTGATATTTGAAAATAATG600.2996703626011388No Hit
GCCCTCGGGAAGAGAGACTTCATACAAGTCCTGCCTTTCAAAGTGTCACTTGGTTGCA570.28468684447108183No Hit
GCCCTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.27469783238437717No Hit
GCCCTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.25471980821096796No Hit
GCCCTCGGGAGCTTCAGGAATCAAAATGTGAAAGATTGAAATATTAATTTATGTTGCT480.23973629008091102No Hit
GCCCTCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT450.22475277195085405No Hit
GCCCTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT360.17980221756068324No Hit
GCCCTCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA360.17980221756068324No Hit
GCCCTCGGGGAGCATCGAGGTTCCTGAGCATGAGTTAAGGGACATCCACAGAAAGCCA350.17480771151733093No Hit
GCCCTCGGGATTGTCTGTTCTATTTATTTTTCCAGTGAAAAGTATTTTGATAGAGCTT330.1648186994306263No Hit
GCCCTCGGGATGTAGCGTTTGCCACAGGTCAGACTGGTTCATTTCCTAGCTAGAAGCC320.159824193387274No Hit
GCCCTCGGGGGAATTCCTTCGGCAACATTAACTGGCTGATGCTGAAAAACATCGAACT310.1548296873439217No Hit
GCCCTCGGGATGGTTGCTTCTGAATTTTTGCATTTATGAAAGCTGTCTCCATTGCTAT310.1548296873439217No Hit
GCCCTCGGGATTCTAAAAGATAAAGTTTGTTCTAAAAACCAGCTGTAGTATTTTTTTT300.1498351813005694No Hit
GCCCTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.1498351813005694No Hit
GCCCTCGGGATTTGAAGGTGGCTCCTGCCACCTGCTAATAGCAGTTCAAACTAAATTT290.14484067525721706No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCTCC290.14484067525721706No Hit
GCCCTCGGGGATAAGATGGCGGCGGACACTGGGAGCTGGTGCGTGTACGCCGTGCTGC290.14484067525721706No Hit
GCCCTCGGGAGCAGGGTCAAGCTACATAGAGATAACTAAGACCTCCTATAGAAAACAA280.13984616921386475No Hit
GCCCTCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG280.13984616921386475No Hit
GCCCTCGGGAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTG280.13984616921386475No Hit
GCCCTCGGGAGCCTTTATGATGTGAGCTCTCAGCTGTTCACACTGCCATGCCTCCGTT270.13485166317051242No Hit
GCCCTCGGGGAATATTCAGTATGGCCTCCTCTTACGTTGGTGAGTCGTCTTGCCAACA270.13485166317051242No Hit
GCCCTCGGGAGCCTGACTAACTCCAAATGATGATGCTCACATGGAAAACGATGAACCA260.12985715712716012No Hit
GCCCTCGGGATGTAAGGACATATAAGTTTGTTCTAGAAAGAAGTAGAATTTCTGTATC260.12985715712716012No Hit
GCCCTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.12486265108380781No Hit
GCCCTCGGGGAGCTGTTGGACCTTGTTGCAAGGAGGGGACAACTGCACAGAGCCAAGA250.12486265108380781No Hit
GCCCTCGGGGATTTTCACAGCATAGCTCCAGGCAGCTGACACTCGCAGTTATTGCAGG250.12486265108380781No Hit
GCCCTCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAA250.12486265108380781No Hit
GCCCTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC240.11986814504045551No Hit
GCCCTCGGGATAATATCTCATTTAAAGGAGGTTCTTTATGAACTTGGTGTCCATTGTC240.11986814504045551No Hit
GCCCTCGGGAGGGATGGTGATTCAGCTAGAAGTTCTTTTATTGAGAATAGCCTTTGCT220.10987913295375089No Hit
GCCCTCGGGGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.10987913295375089No Hit
GCCCTCGGGATACAAAACTGTGACAGAGTTATCTAGTTATGTTTGTCTTTGTGAATAG210.10488462691039856No Hit
GCCCTCGGGAACCACACTGCTACCTCCCAGATCAAGGGAGTACTTTTTCTCCGTAAAA210.10488462691039856No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTGG150.00703853952.1457327
CTACAGC150.00703853952.1457351
TAGAACA150.00703853952.1457322
CTCCTGG150.00703853952.1457346
TCAGGTC150.00703853952.1457345
ACTGAAC600.052.1457349
ACATCGA307.456747E-752.1457350
ACATTAA307.456747E-752.1457326
GGCCCTT150.00703853952.1457323
CATTAAC307.456747E-752.1457327
CTGAACC600.052.1457350
CCTTTTG150.00703853952.1457326
CGCAGGC307.456747E-752.1457346
GCGCAGG307.456747E-752.1457345
CCATACC150.00703853952.1457345
ATACCTA150.00703853952.1457347
CAGGCCG353.5517587E-852.1457348
CAGGTCT150.00703853952.1457346
TACCTAC150.00703853952.1457348
CCCTTTT150.00703853952.1457325
Produced by FastQC (version 0.11.5)