FastQCFastQC Report
Tue 31 May 2016
SRR212565_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212565_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21024
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGATGTGAGTTAAAGTCAGTCAGTGCTTCTGTCTGCTTGAGCTACTTACCT3891.8502663622526636No Hit
GCCCTCGGGATCACAGGCAGTCCTCAGCTGCCTGTGTGGGTGCTGGGAACTGAACCTG3491.6600076103500763No Hit
GCCCTCGGGAGAACATACACATAGAACATACACACACACAGAACATACAGAACATACA1810.8609208523592085No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC1690.8038432267884322No Hit
GCCCTCGGGAGCAGGACCTAGGACTCCTCTCCTACCTCTTTTGAAAGGTTCTTTTCGT1660.7895738203957382No Hit
GCCCTCGGGATGTGAATTTTTAAGCTGCGAATCTGATGGCCTTAATTTCCTTTTTGAC1300.6183409436834094No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC1250.594558599695586No Hit
GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT1240.5898021308980212No Hit
GCCCTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC1140.5422374429223744No Hit
GCCCTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG1100.5232115677321156No Hit
GCCCTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC840.3995433789954338No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC730.3472222222222222No Hit
GCCCTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.3329528158295281No Hit
GCCCTCGGGATCACAGGCAGTCCTCAGCTGCCTGTGTGGGGGCTGGGAACTGAACCTG660.3139269406392694No Hit
GCCCTCGGGAGACCAGCTGTGTGGCCCTTTTGGTTTTGGAGACCCCATACCTACAGCT650.3091704718417047No Hit
GCCCTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA630.2996575342465753No Hit
GCCCTCGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC600.28538812785388123No Hit
GCCCTCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT590.2806316590563166No Hit
GCCCTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC520.24733637747336376No Hit
GCCCTCGGGAAATTGAACCAGCTGCAGTCAGTTGCCGTGAAATTAAGAGAAGCAGAAA490.2330669710806697No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCTCC490.2330669710806697No Hit
GCCCTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.22355403348554032No Hit
GCCCTCGGGGGAATTCCTTCGGCAACATTAACTGGCTGATGCTGAAAAACATCGAACT460.21879756468797562No Hit
GCCCTCGGGAGCTTCAGGAATCAAAATGTGAAAGATTGAAATATTAATTTATGTTGCT460.21879756468797562No Hit
GCCCTCGGGAGCAAGACATGTATTCTTAATATGTAATTTGTGATATTTGAAAATAATG420.1997716894977169No Hit
GCCCTCGGGAGTATTGCTGGGTTTAATTTTTCCTACTATTATTGATTCTTGATATTCA410.1950152207001522No Hit
GCCCTCGGGAAGAGAGACTTCATACAAGTCCTGCCTTTCAAAGTGTCACTTGGTTGCA390.1855022831050228No Hit
GCCCTCGGGAGCCTTTATGATGTGAGCTCTCAGCTGTTCACACTGCCATGCCTCCGTT390.1855022831050228No Hit
GCCCTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.1855022831050228No Hit
GCCCTCGGGGAATATTCAGTATGGCCTCCTCTTACGTTGGTGAGTCGTCTTGCCAACA360.17123287671232876No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCCCC340.1617199391171994No Hit
GCCCTCGGGAGTGTGGTGGCTGGCTTCCAGTGGGCTACTAAGGAGGGCGCTCTCTGTG320.15220700152207No Hit
GCCCTCGGGATGGTTGCTTCTGAATTTTTGCATTTATGAAAGCTGTCTCCATTGCTAT320.15220700152207No Hit
GCCCTCGGGATTGTCTGTTCTATTTATTTTTCCAGTGAAAAGTATTTTGATAGAGCTT310.1474505327245053No Hit
GCCCTCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA310.1474505327245053No Hit
GCCCTCGGGATTCTAAAAGATAAAGTTTGTTCTAAAAACCAGCTGTAGTATTTTTTTT300.14269406392694062No Hit
GCCCTCGGGATGTAGCGTTTGCCACAGGTCAGACTGGTTCATTTCCTAGCTAGAAGCC300.14269406392694062No Hit
GCCCTCGGGGATAAGATGGCGGCGGACACTGGGAGCTGGTGCGTGTACGCCGTGCTGC300.14269406392694062No Hit
GCCCTCGGGGATTTTCACAGCATAGCTCCAGGCAGCTGACACTCGCAGTTATTGCAGG290.13793759512937595No Hit
GCCCTCGGGAGCCTGACTAACTCCAAATGATGATGCTCACATGGAAAACGATGAACCA270.12842465753424656No Hit
GCCCTCGGGAGCAGGGTCAAGCTACATAGAGATAACTAAGACCTCCTATAGAAAACAA270.12842465753424656No Hit
GCCCTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT260.12366818873668188No Hit
GCCCTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC260.12366818873668188No Hit
GCCCTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.12366818873668188No Hit
GCCCTCGGGAGTTCTGCCCAGCCCTGACCTGTCCTTAGATAACAGAAACATCTTTGAT250.1189117199391172No Hit
GCCCTCGGGATTTGAAGGTGGCTCCTGCCACCTGCTAATAGCAGTTCAAACTAAATTT250.1189117199391172No Hit
GCCCTCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA240.1141552511415525No Hit
GCCCTCGGGAACCACACTGCTACCTCCCAGATCAAGGGAGTACTTTTTCTCCGTAAAA240.1141552511415525No Hit
GCCCTCGGGATGTCAGTTTACTCTGTAAGCCCAGAAAAATACCGAACACTCTGCTCCA240.1141552511415525No Hit
GCCCTCGGGGATTGTTTCCCATGTGTAACGTTTCGGTTGAGATGTTAACTGGTGGACG240.1141552511415525No Hit
GCCCTCGGGATACAAAACTGTGACAGAGTTATCTAGTTATGTTTGTCTTTGTGAATAG230.10939878234398781No Hit
GCCCTCGGGATGTAAGGACATATAAGTTTGTTCTAGAAAGAAGTAGAATTTCTGTATC230.10939878234398781No Hit
GCCCTCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG230.10939878234398781No Hit
GCCCTCGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTTTTCTTCCGCCCC230.10939878234398781No Hit
GCCCTCGGGATAATATCTCATTTAAAGGAGGTTCTTTATGAACTTGGTGTCCATTGTC230.10939878234398781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGC650.052.2829785
ACAGTGT150.006970423352.28297410
AGCTCGA150.006970423352.28297410
GACAGTG203.2805811E-452.2829749
CGGGGTG458.0035534E-1152.2829746
CGGGGTA150.006970423352.2829746
CGGGGGT251.5487922E-552.2829746
CGGGGGG1950.052.2829746
CGGGGGA1100.052.2829746
CGGGGCT401.6625563E-952.2829746
CGGGGAC150.006970423352.2829746
GAGCTTC150.006970423352.2829749
GGATTCT150.006970423352.2829748
GGACAGT251.5487922E-552.2829748
GATTGTC150.006970423352.2829749
GGGAGGA150.006970423352.2829747
CGGGCTT150.006970423352.2829746
CGGGCTC150.006970423352.2829746
GGGAGAC203.2805811E-452.2829747
CGGGTGT150.006970423352.2829746
Produced by FastQC (version 0.11.5)