FastQCFastQC Report
Tue 31 May 2016
SRR212564_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212564_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44054
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT13022.955463749035275No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6571.4913515231307033No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5131.1644799564171244No Hit
GCCCTCGGGATGTGAGTTAAAGTCAGTCAGTGCTTCTGTCTGCTTGAGCTACTTAC4661.0577927089481092No Hit
GCCCTCGGGATCACAGGCAGTCCTCAGCTGCCTGTGTGGGTGCTGGGAACTGAACC3500.7944795024288372No Hit
GCCCTCGGGAGCAGGACCTAGGACTCCTCTCCTACCTCTTTTGAAAGGTTCTTTTC3490.7922095609933264No Hit
GCCCTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2960.6719026649112453No Hit
GCCCTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA2800.6355836019430698No Hit
GCCCTCGGGAGTATTGCTGGGTTTAATTTTTCCTACTATTATTGATTCTTGATATT2670.6060743632814274No Hit
GCCCTCGGGATGTGAATTTTTAAGCTGCGAATCTGATGGCCTTAATTTCCTTTTTG1920.4358287556181051No Hit
GCCCTCGGGAGAACATACACATAGAACATACACACACACAGAACATACAGAACATA1790.4063195169564625No Hit
GCCCTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG1670.37908021973033096No Hit
GCCCTCGGGCAGTTGTTAGTCCTGACATAGGTGCTAGGAACTGACCTTGAGTCTTC1550.3518409225041994No Hit
GCCCTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA1400.3177918009715349No Hit
GCCCTCGGGGTTCTTTTCTTTCTCTCATAATCCATTCTTTTTTTTGTTTTTGTTTT1390.31552185953602396No Hit
GCCCTCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1320.2996322694874472No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1310.29736232805193624No Hit
GCCCTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.28374267943887044No Hit
GCCCTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.23834385072865122No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT1020.23153402642211832No Hit
GCCCTCGGGGGAATTCCTTCGGCAACATTAACTGGCTGATGCTGAAAAACATCGAA980.22245426068007446No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC900.20429472919598676No Hit
GCCCTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC900.20429472919598676No Hit
GCCCTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT810.18386525627638808No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC810.18386525627638808No Hit
GCCCTCGGGAGACCAGCTGTGTGGCCCTTTTGGTTTTGGAGACCCCATACCTACAG770.17478549053434422No Hit
GCCCTCGGGGGCAGCATTTGTCTTGATATTCTAAGATCACAGTGGTCTCCTGCTTT770.17478549053434422No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC750.1702456076633223No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC730.16570572479230036No Hit
GCCCTCGGGAGGCAGAATACCCCGATGAATATCATGGCGAGTACCCAGACGACTAC710.16116584192127842No Hit
GCCCTCGGGAGCAAGACATGTATTCTTAATATGTAATTTGTGATATTTGAAAATAA700.15889590048576746No Hit
GCCCTCGGGAGCCTTTATGATGTGAGCTCTCAGCTGTTCACACTGCCATGCCTCCG690.1566259590502565No Hit
GCCCTCGGGAAATTGAACCAGCTGCAGTCAGTTGCCGTGAAATTAAGAGAAGCAGA690.1566259590502565No Hit
GCCCTCGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCC670.15208607617923459No Hit
GCCCTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA660.1498161347437236No Hit
GCCCTCGGGATCACAGGCAGTCCTCAGCTGCCTGTGTGGGGGCTGGGAACTGAACC620.14073636900167977No Hit
GCCCTCGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC620.14073636900167977No Hit
GCCCTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG610.1384664275661688No Hit
GCCCTCGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC610.1384664275661688No Hit
GCCCTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA610.1384664275661688No Hit
GCCCTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.13619648613065782No Hit
GCCCTCGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCTCTTC570.12938666182412492No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC550.124846778953103No Hit
GCCCTCGGGATTCTAAAAGATAAAGTTTGTTCTAAAAACCAGCTGTAGTATTTTTT540.12257683751759205No Hit
GCCCTCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.12030689608208107No Hit
GCCCTCGGGAGCTTCAGGAATCAAAATGTGAAAGATTGAAATATTAATTTATGTTG520.11803695464657012No Hit
GCCCTCGGGAGGGATGGTGATTCAGCTAGAAGTTCTTTTATTGAGAATAGCCTTTG520.11803695464657012No Hit
GCCCTCGGGCACCTGAATCTCCCTCTGTTCTGGGATCCTGCTCGCGCTGGTGTTCG510.11576701321105916No Hit
GCCCTCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC490.11122713034003723No Hit
GCCCTCGGGAAGAGAGACTTCATACAAGTCCTGCCTTTCAAAGTGTCACTTGGTTG480.10895718890452627No Hit
GCCCTCGGGATTTCAAAAGGGAACTATTTAAGGTCTTTAATAAAACCCTCAAAATA460.10441730603350434No Hit
GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG460.10441730603350434Illumina Single End Adapter 2 (95% over 23bp)
GCCCTCGGGATGGTTGCTTCTGAATTTTTGCATTTATGAAAGCTGTCTCCATTGCT460.10441730603350434No Hit
GCCCTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG450.10214736459799338No Hit
GCCCTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.10214736459799338No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGCT203.9983494E-450.3524750
CTGAACC800.050.3524750
CTTTTTG203.9983494E-450.3524750
CATCGAA203.9983494E-450.3524750
CTTGATA354.901449E-850.29472748
AGCCCGA150.008184697550.29472448
AGAAGCA150.008184697550.29472448
ACTGAAC800.050.29472449
GCTACTT550.050.29472448
TTGATAT505.456968E-1250.29472449
GCCCGAA150.008184697550.29472449
CTACTTA550.050.29472449
TGAGTCT251.9841053E-550.29472448
CCAACTG251.997641E-550.23711444
GAGAAGC150.00822192250.23711447
TGACCTT251.997641E-550.23711442
TACAGAA251.997641E-550.23711446
TGACTGT150.00822192250.23711445
CTGGGAA800.050.23711443
AGCTACT550.050.23711447
Produced by FastQC (version 0.11.5)