SRR212563_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212563_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	43285
Sequences flagged as poor quality	0
Sequence length	56
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	1315	3.0380039274575488	No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	603	1.393092295252397	No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	556	1.2845096453736862	No Hit
GCCCTCGGGATGTGAGTTAAAGTCAGTCAGTGCTTCTGTCTGCTTGAGCTACTTAC	431	0.9957260020792422	No Hit
GCCCTCGGGATCACAGGCAGTCCTCAGCTGCCTGTGTGGGTGCTGGGAACTGAACC	405	0.935659004273998	No Hit
GCCCTCGGGAGCAGGACCTAGGACTCCTCTCCTACCTCTTTTGAAAGGTTCTTTTC	350	0.8085942012244427	No Hit
GCCCTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	324	0.7485272034191983	No Hit
GCCCTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	293	0.6769088598821762	No Hit
GCCCTCGGGAGTATTGCTGGGTTTAATTTTTCCTACTATTATTGATTCTTGATATT	231	0.5336721728081322	No Hit
GCCCTCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	182	0.4204689846367102	No Hit
GCCCTCGGGATGTGAATTTTTAAGCTGCGAATCTGATGGCCTTAATTTCCTTTTTG	158	0.36502252512417693	No Hit
GCCCTCGGGGTTCTTTTCTTTCTCTCATAATCCATTCTTTTTTTTGTTTTTGTTTT	157	0.3627122559778214	No Hit
GCCCTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	146	0.33729929536791037	No Hit
GCCCTCGGGCAGTTGTTAGTCCTGACATAGGTGCTAGGAACTGACCTTGAGTCTTC	139	0.32112741134342154	No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	138	0.3188171421970659	No Hit
GCCCTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	136	0.3141966039043549	No Hit
GCCCTCGGGAGAACATACACATAGAACATACACACACACAGAACATACAGAACATA	133	0.30726579646528823	No Hit
GCCCTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	130	0.3003349890262216	No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC	106	0.24488852951368834	No Hit
GCCCTCGGGGGAATTCCTTCGGCAACATTAACTGGCTGATGCTGAAAAACATCGAA	103	0.23795772207462168	No Hit
GCCCTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC	101	0.2333371837819106	No Hit
GCCCTCGGGGGCAGCATTTGTCTTGATATTCTAAGATCACAGTGGTCTCCTGCTTT	101	0.2333371837819106	No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC	100	0.23102691463555503	No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	92	0.21254476146471066	No Hit
GCCCTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	88	0.20330368487928843	No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT	83	0.1917523391475107	No Hit
GCCCTCGGGAGGCAGAATACCCCGATGAATATCATGGCGAGTACCCAGACGACTAC	82	0.18944207000115512	No Hit
GCCCTCGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCC	77	0.17789072426937738	No Hit
GCCCTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	72	0.1663393785375996	No Hit
GCCCTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	72	0.1663393785375996	No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC	68	0.15709830195217744	No Hit
GCCCTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA	65	0.1501674945131108	No Hit
GCCCTCGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCTCTTC	63	0.14554695622039968	No Hit
GCCCTCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC	63	0.14554695622039968	No Hit
GCCCTCGGGATCACAGGCAGTCCTCAGCTGCCTGTGTGGGGGCTGGGAACTGAACC	61	0.1409264179276886	No Hit
GCCCTCGGGAAATTGAACCAGCTGCAGTCAGTTGCCGTGAAATTAAGAGAAGCAGA	61	0.1409264179276886	No Hit
GCCCTCGGGAGACCAGCTGTGTGGCCCTTTTGGTTTTGGAGACCCCATACCTACAG	60	0.13861614878133302	No Hit
GCCCTCGGGAGCCTTTATGATGTGAGCTCTCAGCTGTTCACACTGCCATGCCTCCG	60	0.13861614878133302	No Hit
GCCCTCGGGAGCAAGACATGTATTCTTAATATGTAATTTGTGATATTTGAAAATAA	60	0.13861614878133302	No Hit
GCCCTCGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	59	0.13630587963497748	No Hit
GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	58	0.13399561048862194	Illumina Single End Adapter 2 (95% over 23bp)
GCCCTCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	55	0.12706480304955528	Illumina Single End Adapter 2 (96% over 30bp)
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC	55	0.12706480304955528	No Hit
GCCCTCGGGAAGAGAGACTTCATACAAGTCCTGCCTTTCAAAGTGTCACTTGGTTG	55	0.12706480304955528	No Hit
GCCCTCGGGATGTAAGGACATATAAGTTTGTTCTAGAAAGAAGTAGAATTTCTGTA	55	0.12706480304955528	No Hit
GCCCTCGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC	54	0.12475453390319972	No Hit
GCCCTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54	0.12475453390319972	No Hit
GCCCTCGGGATGCTGTGCCATTTTGATTTTTATTTTGGTTGGTTGGTTGAATAAAT	53	0.12244426475684417	No Hit
GCCCTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	51	0.11782372646413307	No Hit
GCCCTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	51	0.11782372646413307	No Hit
GCCCTCGGGAGCTTCAGGAATCAAAATGTGAAAGATTGAAATATTAATTTATGTTG	46	0.10627238073235533	No Hit
GCCCTCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46	0.10627238073235533	No Hit
GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCCGGCCGCC	45	0.10396211158599977	No Hit
GCCCTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45	0.10396211158599977	No Hit
GCCCTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	44	0.10165184243964422	No Hit
GCCCTCGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT	44	0.10165184243964422	No Hit
GCCCTCGGGGATAAGATGGCGGCGGACACTGGGAGCTGGTGCGTGTACGCCGTGCT	44	0.10165184243964422	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGCGT	20	4.0388788E-4	50.247665	33
AACTGAA	65	0.0	50.247665	48
CCACGCC	20	4.0388788E-4	50.247665	43
GCGGTCG	20	4.0388788E-4	50.247665	33
GCGCGTG	20	4.0388788E-4	50.247665	34
GAGCTAC	20	4.0388788E-4	50.247665	46
TGGAGGC	20	4.0388788E-4	50.247665	35
TTGAGCT	20	4.0388788E-4	50.247665	44
GCTACTT	20	4.0388788E-4	50.247665	48
CGCAGGC	40	2.4683686E-9	50.247665	46
GAACAGG	20	4.0388788E-4	50.247665	49
GCTTGAG	20	4.0388788E-4	50.247665	42
CGAGCGA	20	4.0388788E-4	50.247665	49
TGGGTGC	65	0.0	50.247665	37
AATATTA	20	4.0388788E-4	50.247665	40
CTTGAGC	20	4.0388788E-4	50.247665	43
CGGTCGG	20	4.0388788E-4	50.247665	34
TGGTGCG	20	4.0388788E-4	50.247665	37
GCCGAGC	20	4.0388788E-4	50.247665	47
TGAGCTA	20	4.0388788E-4	50.247665	45

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content