##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212563_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43285 Sequences flagged as poor quality 0 Sequence length 56 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.94402217858381 39.0 38.0 39.0 35.0 40.0 2 37.663001039621115 39.0 38.0 39.0 35.0 40.0 3 37.995633591313386 39.0 38.0 39.0 35.0 40.0 4 38.14448423241308 39.0 38.0 39.0 35.0 40.0 5 37.34808825228139 39.0 38.0 39.0 33.0 40.0 6 38.012775788379344 39.0 38.0 39.0 35.0 40.0 7 38.01395402564399 39.0 38.0 39.0 35.0 40.0 8 38.036432944438026 39.0 38.0 39.0 35.0 40.0 9 38.039598013168536 39.0 38.0 39.0 35.0 40.0 10 36.63904354857341 38.0 36.0 39.0 31.0 40.0 11 36.989003118863344 39.0 36.0 39.0 33.0 40.0 12 36.740510569481344 38.0 35.0 39.0 32.0 40.0 13 36.652743444611296 38.0 35.0 39.0 32.0 40.0 14 36.579923761118174 38.0 35.0 39.0 32.0 40.0 15 36.71403488506411 38.0 36.0 39.0 32.0 40.0 16 36.50028878364329 38.0 36.0 39.0 32.0 40.0 17 36.43922837010512 38.0 36.0 39.0 31.0 40.0 18 36.279103615571216 38.0 35.0 39.0 31.0 40.0 19 35.91916368256902 38.0 35.0 39.0 30.0 40.0 20 35.97421739632667 38.0 35.0 39.0 30.0 40.0 21 35.746956220399674 38.0 35.0 39.0 30.0 40.0 22 35.55226983943629 38.0 35.0 39.0 29.0 40.0 23 35.26055215432598 38.0 35.0 39.0 29.0 40.0 24 35.21349197181472 38.0 35.0 39.0 29.0 40.0 25 35.24130761233684 38.0 35.0 39.0 29.0 40.0 26 34.58311193254014 38.0 34.0 39.0 27.0 40.0 27 34.501397712833544 38.0 33.0 39.0 27.0 40.0 28 33.910939124407996 37.0 33.0 39.0 25.0 40.0 29 34.033545108005086 38.0 33.0 39.0 26.0 40.0 30 33.44932424627469 37.0 33.0 39.0 23.0 40.0 31 32.80351160910246 37.0 32.0 39.0 20.0 40.0 32 32.25406029802472 36.0 31.0 39.0 17.0 39.0 33 32.533371837819104 36.0 32.0 39.0 18.0 39.0 34 32.547279658080164 36.0 32.0 39.0 18.0 40.0 35 31.70922952523969 36.0 30.0 39.0 11.0 40.0 36 32.08277694351392 37.0 32.0 39.0 2.0 40.0 37 31.582026106041354 36.0 31.0 39.0 2.0 40.0 38 31.55989372761927 36.0 31.0 39.0 2.0 40.0 39 31.458911863232068 36.0 31.0 39.0 2.0 40.0 40 31.31689961880559 36.0 31.0 39.0 2.0 40.0 41 30.842162411920988 36.0 30.0 39.0 2.0 39.0 42 30.909991914057986 36.0 30.0 39.0 2.0 39.0 43 30.80064687536098 36.0 30.0 39.0 2.0 39.0 44 30.522860113203187 36.0 29.0 39.0 2.0 39.0 45 30.39330021947557 36.0 30.0 39.0 2.0 39.0 46 29.68739748180663 35.0 29.0 39.0 2.0 39.0 47 29.545038697008202 35.0 28.0 39.0 2.0 39.0 48 29.3792075776828 35.0 28.0 39.0 2.0 39.0 49 29.124269377382465 35.0 27.0 39.0 2.0 39.0 50 28.89070116668592 35.0 27.0 39.0 2.0 39.0 51 28.143121173616727 35.0 25.0 39.0 2.0 39.0 52 28.006722883215893 35.0 24.0 39.0 2.0 39.0 53 27.53339494051057 35.0 23.0 38.0 2.0 39.0 54 27.200277232297562 35.0 18.0 38.0 2.0 39.0 55 26.87732470832852 35.0 2.0 38.0 2.0 39.0 56 15.973593623657155 20.0 2.0 24.0 2.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.1713362256927624 1 2 -0.9306292593549657 1 3 -1.2869332429235811 1 4 -1.2326872705289702 1 5 -0.6187525441272683 1 6 -1.166176926569463 1 7 -1.1204055781039273 1 8 -2.548754834822134 1 9 -2.2336916900291683 1 10 -1.57180948515731 1 11 -1.6283850633327575 1 12 -1.1856547326962357 1 13 -1.8332670874434314 1 14 -1.8288040538385317 1 15 -1.71832510328273 1 16 -1.9545168582550971 1 17 -1.0931070438598525 1 18 -0.8549201515764651 1 19 -1.889807646254006 1 20 -1.5326235290135486 1 21 -0.09564428073652209 1 22 0.33036886141619703 1 23 -0.38526939717110054 1 24 -1.3638623109990746 1 25 -0.8038971532605927 1 26 0.7987132085427433 1 27 -0.13694734626142946 1 28 0.6377293779224047 1 29 0.8049032530805533 1 30 0.5334316042257186 1 31 -0.8218393325784064 1 32 -1.358967638827064 1 33 -0.4959486447335806 1 34 -1.0878458081379137 1 35 -0.2789659176304333 1 36 -0.3505654211724334 1 37 -0.1432208756498774 1 38 -0.6017860455891828 1 39 0.7063491009023721 1 40 -0.8266337957365089 1 41 -1.3867634434517697 1 42 0.4740436544651523 1 43 -0.5695528580937683 1 44 0.029520096521903127 1 45 0.27500502005795724 1 46 0.01587915421154662 1 47 0.34104754059892883 1 48 -0.7107773039649281 1 49 0.0812787118625451 1 50 -0.7838470928651908 1 51 -0.16587723349044126 1 52 -0.24957250148518995 1 53 -0.7606761620006814 1 54 -0.9290720057114079 1 55 0.22919522426952454 1 56 -0.5487901847027583 2 1 -0.7290300746735383 2 2 -0.6646951934209042 2 3 -0.5081786641690016 2 4 -0.6766433144850126 2 5 -1.483954009328734 2 6 -0.19108535147788785 2 7 -0.2793799370782821 2 8 0.054175568108263406 2 9 -0.2783803347178093 2 10 -0.6853625987104195 2 11 -0.5719748069225048 2 12 0.07808153104002713 2 13 0.06783181365546653 2 14 -0.2324670575015304 2 15 -0.14250092745855625 2 16 0.4689263651881248 2 17 0.4761237253709112 2 18 0.4945303978740867 2 19 0.2017674453210887 2 20 -0.2798762762662932 2 21 0.9717549866627451 2 22 0.8644347954821328 2 23 0.32242291052120464 2 24 1.2866871395503736 2 25 0.5008647515013109 2 26 1.3628157726453054 2 27 1.013968404654321 2 28 1.540293480486504 2 29 0.8232182713955751 2 30 1.6030286738227915 2 31 -0.6841103948494691 2 32 0.6109957311363026 2 33 0.48793413914920336 2 34 0.9773556570635549 2 35 0.5415469028823878 2 36 1.2552953846883739 2 37 1.2157534833244803 2 38 1.5535253097221755 2 39 2.0916970862503597 2 40 0.9367361676334554 2 41 -0.23584769253601934 2 42 0.055728636150131905 2 43 -0.11021219875311061 2 44 0.4991171661189746 2 45 0.9497302947832331 2 46 0.7616666999990933 2 47 0.6619266614780521 2 48 1.2174278242402004 2 49 -0.032274401690568055 2 50 1.202966093947996 2 51 1.6788114111982004 2 52 1.7951161432034546 2 53 2.8708256695011514 2 54 2.1112210345816322 2 55 3.136154931229232 2 56 1.821173185260614 3 1 -0.4361825712997245 3 2 -0.42987737965572137 3 3 -0.5230234684874944 3 4 -0.16652185699513922 3 5 -1.067624724578394 3 6 -0.9616656483739732 3 7 -0.8151424202091846 3 8 -0.7359728799349199 3 9 -0.8141428178487118 3 10 -0.8763207633527941 3 11 -3.3547008528064453 3 12 -1.8773840560045087 3 13 -1.837026485939674 3 14 -1.6460972869212327 3 15 -2.9724604416285985 3 16 -2.325945429683671 3 17 -2.7667912543861704 3 18 -2.7504088733809766 3 19 -2.0890557665547576 3 20 -3.3183378147278333 3 21 -1.9648172130673487 3 22 -3.0809093340725298 3 23 -2.906322028750047 3 24 -1.2315314839314055 3 25 -2.75352121341097 3 26 -2.806549949351993 3 27 -2.5076240379907553 3 28 -2.8517443062881256 3 29 -2.9507358446638072 3 30 -3.3011548619396933 3 31 -2.136876926563371 3 32 -2.9018247816842084 3 33 -2.3636178176659115 3 34 -2.3863420487394187 3 35 -1.8842290755251696 3 36 -1.3513173008716812 3 37 -2.2484840335446137 3 38 -2.0085529628824155 3 39 -1.8786133051126654 3 40 -2.067987179195157 3 41 -2.310071714128462 3 42 -1.1703172477904857 3 43 -1.630455113732868 3 44 -2.1682242643803527 3 45 -1.475370919791665 3 46 -3.186376484886196 3 47 -3.3198547150401687 3 48 -2.805514146070191 3 49 -3.7495483558066276 3 50 -3.859786942489251 3 51 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4 37 2.1424934100644073 4 38 2.4267853829822457 4 39 3.6206348151880867 4 40 2.9733662042634883 4 41 1.9560936994053755 4 42 0.6454722258937231 4 43 1.7447328561919484 4 44 2.34380581080762 4 45 2.389290734343671 4 46 0.7587362970686904 4 47 1.683904683456074 4 48 1.3177941246065004 4 49 2.36699299757683 4 50 1.2161529071348092 4 51 0.5484084807952705 4 52 0.9789989270862414 4 53 1.2964666951421755 4 54 1.4423565657171622 4 55 2.2863380814123815 4 56 1.222638386725814 5 1 -0.39777574341920996 5 2 -0.18236671109242053 5 3 -0.6197517757421167 5 4 -0.6828803207220204 5 5 -0.5477100730847937 5 6 -0.1252502856428208 5 7 -0.21770287540122268 5 8 0.05278956672226798 5 9 -0.4869735433110165 5 10 -0.5100334233812447 5 11 -0.3959526309003252 5 12 -0.2899018369433435 5 13 -1.331336585512929 5 14 -1.2172210422555167 5 15 0.260825475867847 5 16 0.02540592166768363 5 17 0.2204064696536605 5 18 0.15357405691774773 5 19 0.6044008479544942 5 20 0.5600405636505457 5 21 0.5344715493793117 5 22 0.27399820504553674 5 23 0.44716303526133316 5 24 0.19521104807428458 5 25 -0.9676037169671616 5 26 -0.7148003049707725 5 27 -1.8411944505085387 5 28 -0.1838922436992192 5 29 0.17942062759792776 5 30 0.09713816793228602 5 31 0.1966934859544125 5 32 0.3954725156130898 5 33 -0.5855239343088705 5 34 -0.4453747656668696 5 35 1.3156286769641632 5 36 0.7119828413758285 5 37 1.1000223675933647 5 38 1.6422293984262595 5 39 1.1256541202073898 5 40 0.5756828065800939 5 41 1.476557019868693 5 42 -0.3004737200522243 5 43 1.3887483002073893 5 44 1.4526861196879253 5 45 0.2116845567374952 5 46 0.5170374553698487 5 47 -1.3817323821809921 5 48 -0.8567232499108748 5 49 -0.07316144257761081 5 50 -0.22322933224743124 5 51 0.3468640792508708 5 52 0.2469525950399074 5 53 1.896466695142177 5 54 2.2408121641727625 5 55 1.9504307455050451 5 56 2.3083526724400993 6 1 -0.646708887089197 6 2 -1.561456327024139 6 3 -0.5493392579611793 6 4 -0.32441659383724186 6 5 -0.5676247245783941 6 6 -0.5669288062687059 6 7 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2.1418542749824425 6 49 2.0662411178775812 6 50 0.4033709522475881 6 51 3.398784420644894 6 52 2.3308786263343606 6 53 2.6859403793527044 6 54 3.2927325055667858 6 55 2.493104998705615 6 56 2.4136158303348356 7 1 0.05943146378799469 7 2 0.662227883502176 7 3 0.5559238999335605 7 4 0.7720746342329363 7 5 0.973310947936227 7 6 0.7298548194622825 7 7 0.5795944218960756 7 8 0.5242610381937354 7 9 0.5805940242565484 7 10 0.821047657699836 7 11 0.36605938111168257 7 12 0.35799606095455516 7 13 0.9802249760486319 7 14 1.0965927715582993 7 15 0.8126272776696482 7 16 1.1462767925385506 7 17 0.623559622806809 7 18 1.135556038899729 7 19 0.8086050521586969 7 20 0.9594399630499453 7 21 0.1170541319618934 7 22 0.037511718559052554 7 23 1.764730602828898 7 24 0.7012170540802884 7 25 0.1119758626124181 7 26 0.9931576529871862 7 27 1.9733701140560242 7 28 1.2155071557001804 7 29 2.0668080149853196 7 30 2.100891921686035 7 31 3.354351143612071 7 32 3.3481752183157916 7 33 0.6524640536791182 7 34 2.186757366465258 7 35 2.7210340823695667 7 36 3.2232441026370893 7 37 3.6623346799056797 7 38 3.412499668696533 7 39 3.2690475136007855 7 40 3.4217789026761913 7 41 5.001331794643466 7 42 4.8676944481159445 7 43 4.159018570477659 7 44 5.024758191759997 7 45 4.853576448629386 7 46 6.342863281195676 7 47 6.219618969170359 7 48 6.64477825159063 7 49 5.390802521386352 7 50 6.254248145230044 7 51 7.014281496668286 7 52 7.63058622867354 7 53 7.452022250697734 7 54 8.158229581590177 7 55 7.405385700460002 7 56 4.308352672440099 8 1 0.003875908232444658 8 2 -0.22666100538670975 8 3 -0.08296498895532523 8 4 -0.4779253657670637 8 5 -0.2211334965082159 8 6 -0.9090340694266033 8 7 -0.6426278003261459 8 8 -0.6424056284729289 8 9 -0.891628197965673 8 10 -0.6233967867446069 8 11 -1.7728295077772032 8 12 -0.8920039390454448 8 13 -1.2975528017291467 8 14 -0.764518339552815 8 15 -1.4651505001081304 8 16 -1.3537232074614494 8 17 -1.7097737105265196 8 18 -0.5311106277669424 8 19 -0.7469505033968602 8 20 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2.1846651279768032 115 42 1.4704722258937224 115 43 1.2090185704776601 115 44 2.058091525093335 115 45 2.703576448629388 115 46 0.9623077256401196 115 47 0.5946189691703587 115 48 2.564222696035074 115 49 2.7241358547196874 115 50 2.1625814785633786 115 51 4.069837052223843 115 52 3.4861417842290976 115 53 3.1714666951421755 115 54 3.363785137145733 115 55 3.7720523671266655 115 56 1.8083526724400993 116 1 0.2966109509674837 116 2 0.5917150629893584 116 3 0.3636162076258671 116 4 0.14386950602780502 116 5 0.1057895804148572 116 6 0.21917105877852805 116 7 0.10523544753710468 116 8 0.2080217219544238 116 9 0.2600812037437308 116 10 0.4428425294947047 116 11 1.1716149366672397 116 12 0.07808153104002713 116 13 0.37552412134777313 116 14 0.7162508912164185 116 15 0.7805759956183707 116 16 0.3663622626240226 116 17 1.2709955202427068 116 18 -0.2490593457156578 116 19 0.2274084709621107 116 20 0.4637134673234513 116 21 0.9461139610217231 116 22 0.992639923687257 116 23 1.1172947053930002 116 24 1.1071999600631983 116 25 1.2444544950910554 116 26 0.8499952598247944 116 27 0.8601222508081676 116 28 0.05311399330701505 116 29 -0.715243267065965 116 30 0.32097739177150686 116 31 0.6748639641248886 116 32 -0.36336324322266833 116 33 -0.5633479121328477 116 34 -0.12520844550054733 116 35 -0.7661454048099188 116 36 0.12709025648324612 116 37 0.779856047427046 116 38 0.6048073610042266 116 39 0.2968252913785605 116 40 0.9623771932744809 116 41 0.4821010254126996 116 42 0.31213889256039096 116 43 -0.08457117311208506 116 44 0.9862966532984601 116 45 -0.8451415000885625 116 46 0.47961541794781226 116 47 0.4055164050677931 116 48 0.21742782424020035 116 49 0.0959307265145597 116 50 0.433735324717226 116 51 0.3967601291469194 116 52 0.6925520406393524 116 53 0.8964666951421769 116 54 0.9573748807354789 116 55 0.5207703158446151 116 56 0.46219882628625264 117 1 -0.3437216893651538 117 2 -0.12831265703836436 117 3 -0.4305625865529237 117 4 -0.06125869910039938 117 5 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0.8720037583937454 119 6 0.6497894599851648 119 7 0.6384179513078436 119 8 0.7856989466904665 119 9 0.7276528477859614 119 10 0.27693001064100997 119 11 -0.24014976921511533 119 12 0.15047972108527574 119 13 -0.3710822134938496 119 14 0.838422836917772 119 15 1.072431199238281 119 16 0.7328780997280973 119 17 0.9127753090813187 119 18 1.1551638820369803 119 19 1.0765789083678428 119 20 1.3287210088015797 119 21 2.002675046994568 119 22 2.1992764244414076 119 23 0.985318838123014 119 24 2.155465420093364 119 25 2.4616490652268013 119 26 2.0928308556015693 119 27 2.1874224016377255 119 28 2.378905848510634 119 29 1.439357034593158 119 30 0.2855324445618521 119 31 1.0210178102787353 119 32 1.5393516889040235 119 33 1.699849674594148 119 34 1.2978684775763725 119 35 2.6916223176636827 119 36 1.924224494793954 119 37 2.2097203008207096 119 38 2.8242643745788882 119 39 1.4634919580452284 119 40 3.057399817708866 119 41 1.9376063044473888 119 42 2.204295755305491 119 43 2.6884303351835435 119 44 2.8580915250933323 119 45 2.5153411545117415 119 46 1.8461312550518834 119 47 1.5872660279938877 119 48 1.3245168136821306 119 49 0.812371148837336 119 50 1.8816991256222018 119 51 1.4080723463414913 119 52 2.2302594312879194 119 53 0.8376431657304124 119 54 1.7431969018516185 119 55 3.1308758965384307 119 56 2.3083526724400993 120 1 -1.9624983607734094 120 2 -1.4035615901820364 120 3 -1.654602415855912 120 4 -1.692837646468817 120 5 -2.3307826193152366 120 6 -2.3564024904792333 120 7 -2.9730371570512943 120 8 -2.9201834062507075 120 9 -2.9983533441645065 120 10 -2.0868470791422666 120 11 -1.6441745370169727 120 12 -1.719489319162406 120 13 -1.4949212227817767 120 14 -2.909255181658075 120 15 -2.7882499153128038 120 16 -2.5101559559994584 120 17 -2.6615280964914376 120 18 -2.145145715486237 120 19 -2.0101083981337027 120 20 -1.8183378147278333 120 21 -1.1227119499094513 120 22 -2.1598567024935775 120 23 -1.8800062392763621 120 24 -1.8894262207735153 120 25 -0.9377317397267646 120 26 -1.1749710019835717 120 27 -0.7181503537802314 120 28 -0.5885864115512831 120 29 -0.13494637097959838 120 30 -0.7485232829923234 120 31 1.5999651786997902 120 32 1.2297541656842093 120 33 2.0837506033867186 120 34 2.1926053196816326 120 35 2.142086713948512 120 36 1.6223669096546374 120 37 1.2778317559290677 120 38 1.7809207213281155 120 39 1.8582288001504885 120 40 1.5899075576469492 120 41 1.8741388121873293 120 42 1.8559985416831957 120 43 1.1590185704776594 120 44 1.3844073145670173 120 45 1.366734343366229 120 46 2.9188866730085365 120 47 3.0485663375914136 120 48 2.4576437486666514 120 49 2.98729374945653 120 50 4.008634110142324 120 51 4.793521262750158 120 52 4.278247047386991 120 53 4.685940379352704 120 54 3.6611535581983645 120 55 3.5194207881792963 120 56 1.4925631987558905 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19.0 3 4.0 4 21.0 5 7.0 6 26.0 7 102.0 8 44.0 9 62.0 10 83.0 11 83.0 12 121.0 13 206.0 14 220.0 15 230.0 16 276.0 17 377.0 18 389.0 19 486.0 20 594.0 21 459.0 22 468.0 23 591.0 24 721.0 25 626.0 26 579.0 27 659.0 28 780.0 29 922.0 30 1154.0 31 1364.0 32 1770.0 33 2328.0 34 2936.0 35 3665.0 36 5037.0 37 7181.0 38 6212.0 39 2483.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 38.61152824304032 46.68129837126025 4.119209887951946 10.587963497747488 11 35.906203072657966 29.05163451542105 19.13826960840938 15.90389280351161 12 46.61430056601594 12.246736744830773 22.43040314196604 18.70855954718725 13 23.2066535751415 18.521427746332446 25.98821762735359 32.283701051172464 14 20.531361903661775 27.672403835046783 30.962227099457085 20.834007161834354 15 32.408455585075664 20.011551345731778 30.172115051403487 17.407878017789074 16 28.529513688344693 20.556774864271688 32.58634630934504 18.32736513803858 17 33.22398059373917 18.11019983828116 24.2254822686843 24.44033729929537 18 32.255977821416195 19.36698625389858 25.108005082592122 23.269030842093102 19 24.632089638442878 26.228485618574567 31.542104655192333 17.597320087790226 20 27.286588887605408 31.91636825690193 22.532054984405683 18.264987871086984 21 27.820261060413536 22.499711216356705 30.61799699665011 19.062030726579646 22 26.186900773940163 20.50363867390551 23.906665126487237 29.40279542566709 23 25.713295598937275 30.816680143236685 25.459165992838166 18.010858264987874 24 26.519579531015363 29.497516460667665 22.38419775903893 21.59870624927804 25 24.247255921432696 23.010976314269207 21.95956094742923 30.78220681686886 26 27.39000762952859 22.726747277645483 26.012068527038583 23.871176565787344 27 20.087871430223146 19.574517285235288 27.619377962770265 32.7182333217713 28 18.31868640148011 28.871415356151715 27.45605920444033 25.353839037927845 29 27.366740699611324 21.885989265223024 30.018045530260967 20.729224504904682 30 24.012594341806732 25.01967865907302 33.83803306014725 17.129693938973006 31 29.970821175489785 17.74813579732296 34.55838080681766 17.722662220369596 32 28.45001273708344 15.56703179639193 38.97315949144299 17.00979597508163 33 28.4999420827059 14.801343681223214 35.853121742152204 20.845592493918687 34 23.864347100928907 18.971947462299337 41.596979313859485 15.566726122912275 35 17.32903853729752 28.232104372813016 40.500081106759666 13.938775983129794 36 22.8758927743252 25.739727298024302 32.761339393377234 18.623040534273258 37 20.808511624672583 22.426462066247886 42.50249182911847 14.262534479961058 38 23.794831345984317 22.82744861504199 38.13854219830186 15.239177840671832 39 23.10512880018566 22.840566256672084 39.579948944070544 14.474355999071712 40 21.47833356555664 19.348845850169525 38.95545956992243 20.2173610143514 41 17.671652884883397 22.247514633466505 41.6426646845675 18.438167797082595 42 20.257458871642346 21.12185147318524 38.64439074263407 19.97629891253834 43 19.154523693323107 15.694066792163424 39.113156243463706 26.038253271049754 44 16.78518311953895 18.741866517940135 42.34987915969511 22.123071202825802 45 23.32566194760211 19.2877234581677 40.26780110188995 17.118813492340237 46 21.17668936981334 21.77874892489365 38.345847183802505 18.698714521490505 47 19.846493778346318 23.546924060937318 39.01383881846726 17.592743342249097 48 18.34213526277831 20.038154619268084 40.79985110392481 20.8198590140288 49 19.43998697644131 20.684201958185074 40.58466475964557 19.29114630572804 50 18.223701049398517 18.53782255625102 42.26214021453335 20.97633617981711 51 17.102447194565926 19.761328742904997 44.85437796594399 18.281846096585095 52 18.017829294974746 18.913949212112747 43.17203174824849 19.896189744664014 53 13.907963036242174 19.65270827029166 45.2759479527944 21.163380740671773 54 15.95781828339968 21.49824242847499 45.81092720627604 16.733012081849292 55 15.323162920694072 19.979038080819844 41.44404332129964 23.253755677186444 56 16.653080237568417 20.479795039012462 36.906952369861415 25.960172353557702 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 10.0 14 20.0 15 38.5 16 57.0 17 68.0 18 79.0 19 103.5 20 128.0 21 193.5 22 334.5 23 410.0 24 407.5 25 405.0 26 462.0 27 519.0 28 659.0 29 1036.0 30 1273.0 31 1477.0 32 1681.0 33 1787.5 34 1894.0 35 1973.0 36 2052.0 37 1676.0 38 1797.0 39 2294.0 40 1655.0 41 1016.0 42 1079.0 43 1142.0 44 963.5 45 785.0 46 782.0 47 925.5 48 1072.0 49 1577.5 50 2083.0 51 1708.5 52 1334.0 53 1502.5 54 1466.5 55 1262.0 56 1374.0 57 1486.0 58 1664.0 59 1842.0 60 1774.0 61 1706.0 62 1920.0 63 2397.0 64 2660.0 65 3048.5 66 3437.0 67 2674.0 68 1911.0 69 1577.0 70 1243.0 71 1081.5 72 819.0 73 718.0 74 559.5 75 401.0 76 329.5 77 258.0 78 174.5 79 77.5 80 64.0 81 49.5 82 35.0 83 28.5 84 22.0 85 25.0 86 28.0 87 17.0 88 5.5 89 5.0 90 4.0 91 3.0 92 2.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.023102691463555505 26 0.07392861268337761 27 0.09241076585422202 28 0.10396211158599977 29 0.1409264179276886 30 0.2102344923183551 31 0.23795772207462168 32 0.24026799122097725 33 0.27723229756266604 34 0.26799122097724387 35 0.30495552731893266 36 0.3719533325632436 37 0.3326787570751992 38 0.4135381771976436 39 0.4505024835393323 40 0.5151900196372877 41 0.5382927111008432 42 0.5752570174425321 43 0.5914289014670209 44 0.5844980940279543 45 0.6191521312232875 46 0.6145315929305765 47 0.6699780524431096 48 0.6977012821993762 49 0.6607369758576874 50 0.7115628970775095 51 0.6861499364675985 52 0.7439066651264872 53 0.7485272034191983 54 0.7577682800046205 55 0.8085942012244427 56 0.8085942012244427 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 43285.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.39239921450849 #Duplication Level Percentage of deduplicated Percentage of total 1 71.37411295525189 28.115975511147052 2 10.92604539323207 8.60806283932078 3 5.366254178640549 6.341688806745986 4 3.237346783179872 5.101074275153055 5 2.0702598088088675 4.077625043317547 6 1.4896487009559556 3.5208501790458593 7 1.0439270424022051 2.878595356359016 8 0.9911442144155769 3.1234838858727043 9 0.5512873145270072 1.9544876978167955 >10 2.6567356753269604 17.645835739863696 >50 0.16421324262506598 4.22086173039159 >100 0.11143041463843761 8.695853066882291 >500 0.011729517330361856 2.677601940626083 >1k 0.005864758665180928 3.0380039274575488 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1315 3.0380039274575488 No Hit GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 603 1.393092295252397 No Hit GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 556 1.2845096453736862 No Hit GCCCTCGGGATGTGAGTTAAAGTCAGTCAGTGCTTCTGTCTGCTTGAGCTACTTAC 431 0.9957260020792422 No Hit GCCCTCGGGATCACAGGCAGTCCTCAGCTGCCTGTGTGGGTGCTGGGAACTGAACC 405 0.935659004273998 No Hit GCCCTCGGGAGCAGGACCTAGGACTCCTCTCCTACCTCTTTTGAAAGGTTCTTTTC 350 0.8085942012244427 No Hit GCCCTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 324 0.7485272034191983 No Hit GCCCTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 293 0.6769088598821762 No Hit GCCCTCGGGAGTATTGCTGGGTTTAATTTTTCCTACTATTATTGATTCTTGATATT 231 0.5336721728081322 No Hit GCCCTCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 182 0.4204689846367102 No Hit GCCCTCGGGATGTGAATTTTTAAGCTGCGAATCTGATGGCCTTAATTTCCTTTTTG 158 0.36502252512417693 No Hit GCCCTCGGGGTTCTTTTCTTTCTCTCATAATCCATTCTTTTTTTTGTTTTTGTTTT 157 0.3627122559778214 No Hit GCCCTCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 146 0.33729929536791037 No Hit GCCCTCGGGCAGTTGTTAGTCCTGACATAGGTGCTAGGAACTGACCTTGAGTCTTC 139 0.32112741134342154 No Hit GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 138 0.3188171421970659 No Hit GCCCTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 136 0.3141966039043549 No Hit GCCCTCGGGAGAACATACACATAGAACATACACACACACAGAACATACAGAACATA 133 0.30726579646528823 No Hit GCCCTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 130 0.3003349890262216 No Hit GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC 106 0.24488852951368834 No Hit GCCCTCGGGGGAATTCCTTCGGCAACATTAACTGGCTGATGCTGAAAAACATCGAA 103 0.23795772207462168 No Hit GCCCTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 101 0.2333371837819106 No Hit GCCCTCGGGGGCAGCATTTGTCTTGATATTCTAAGATCACAGTGGTCTCCTGCTTT 101 0.2333371837819106 No Hit GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC 100 0.23102691463555503 No Hit GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 92 0.21254476146471066 No Hit GCCCTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 88 0.20330368487928843 No Hit GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 83 0.1917523391475107 No Hit GCCCTCGGGAGGCAGAATACCCCGATGAATATCATGGCGAGTACCCAGACGACTAC 82 0.18944207000115512 No Hit GCCCTCGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCC 77 0.17789072426937738 No Hit GCCCTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 72 0.1663393785375996 No Hit GCCCTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 72 0.1663393785375996 No Hit GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC 68 0.15709830195217744 No Hit GCCCTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA 65 0.1501674945131108 No Hit GCCCTCGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCTCTTC 63 0.14554695622039968 No Hit GCCCTCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC 63 0.14554695622039968 No Hit GCCCTCGGGATCACAGGCAGTCCTCAGCTGCCTGTGTGGGGGCTGGGAACTGAACC 61 0.1409264179276886 No Hit GCCCTCGGGAAATTGAACCAGCTGCAGTCAGTTGCCGTGAAATTAAGAGAAGCAGA 61 0.1409264179276886 No Hit GCCCTCGGGAGACCAGCTGTGTGGCCCTTTTGGTTTTGGAGACCCCATACCTACAG 60 0.13861614878133302 No Hit GCCCTCGGGAGCCTTTATGATGTGAGCTCTCAGCTGTTCACACTGCCATGCCTCCG 60 0.13861614878133302 No Hit GCCCTCGGGAGCAAGACATGTATTCTTAATATGTAATTTGTGATATTTGAAAATAA 60 0.13861614878133302 No Hit GCCCTCGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 59 0.13630587963497748 No Hit GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG 58 0.13399561048862194 Illumina Single End Adapter 2 (95% over 23bp) GCCCTCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG 55 0.12706480304955528 Illumina Single End Adapter 2 (96% over 30bp) GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC 55 0.12706480304955528 No Hit GCCCTCGGGAAGAGAGACTTCATACAAGTCCTGCCTTTCAAAGTGTCACTTGGTTG 55 0.12706480304955528 No Hit GCCCTCGGGATGTAAGGACATATAAGTTTGTTCTAGAAAGAAGTAGAATTTCTGTA 55 0.12706480304955528 No Hit GCCCTCGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC 54 0.12475453390319972 No Hit GCCCTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 54 0.12475453390319972 No Hit GCCCTCGGGATGCTGTGCCATTTTGATTTTTATTTTGGTTGGTTGGTTGAATAAAT 53 0.12244426475684417 No Hit GCCCTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 51 0.11782372646413307 No Hit GCCCTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 51 0.11782372646413307 No Hit GCCCTCGGGAGCTTCAGGAATCAAAATGTGAAAGATTGAAATATTAATTTATGTTG 46 0.10627238073235533 No Hit GCCCTCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46 0.10627238073235533 No Hit GCCCTCGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCCGGCCGCC 45 0.10396211158599977 No Hit GCCCTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45 0.10396211158599977 No Hit GCCCTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 44 0.10165184243964422 No Hit GCCCTCGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT 44 0.10165184243964422 No Hit GCCCTCGGGGATAAGATGGCGGCGGACACTGGGAGCTGGTGCGTGTACGCCGTGCT 44 0.10165184243964422 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.013861614878133302 0.0 0.0 0.0 0.0 11 0.030033498902622156 0.0 0.0 0.0 0.0 12 0.030033498902622156 0.0 0.0 0.0 0.0 13 0.030033498902622156 0.0 0.0 0.0 0.0 14 0.036964306341688805 0.0 0.0 0.0 0.0 15 0.04158484463439991 0.0 0.0 0.0 0.0 16 0.04620538292711101 0.0 0.0 0.0 0.0 17 0.04620538292711101 0.0 0.0 0.0 0.0 18 0.04620538292711101 0.0 0.0 0.0 0.0 19 0.04620538292711101 0.0 0.0 0.0 0.0 20 0.05775672865888876 0.0 0.0 0.0 0.0 21 0.05775672865888876 0.0 0.0 0.0 0.0 22 0.07161834353702207 0.0 0.0 0.0 0.0 23 0.07854915097608871 0.0 0.0 0.0 0.0 24 0.09934157329328867 0.0 0.0 0.0 0.0 25 0.09934157329328867 0.0 0.0 0.0 0.0 26 0.09934157329328867 0.0 0.0 0.0 0.0 27 0.09934157329328867 0.0 0.0 0.0 0.0 28 0.20792422317199954 0.0 0.0 0.0 0.0 29 0.21254476146471063 0.0 0.0 0.0 0.0 30 0.2425782603673328 0.0 0.0 0.0 0.0 31 0.24488852951368834 0.0 0.0 0.0 0.0 32 0.24488852951368834 0.0 0.0 0.0 0.0 33 0.2471987986600439 0.0 0.0 0.0 0.0 34 0.2471987986600439 0.0 0.0 0.0 0.0 35 0.344230102806977 0.0 0.0 0.0 0.0 36 0.344230102806977 0.0 0.0 0.0 0.0 37 0.34654037195333254 0.0 0.0 0.0 0.0 38 0.34654037195333254 0.0 0.0 0.0 0.0 39 0.34654037195333254 0.0 0.0 0.0 0.0 40 0.34654037195333254 0.0 0.0 0.0 0.0 41 0.3511609102460437 0.0 0.0 0.0 0.0 42 0.3534711793923992 0.0 0.0 0.0 0.0 43 0.3534711793923992 0.0 0.0 0.0 0.0 44 0.3627122559778214 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGCGT 20 4.0388788E-4 50.247665 33 AACTGAA 65 0.0 50.247665 48 CCACGCC 20 4.0388788E-4 50.247665 43 GCGGTCG 20 4.0388788E-4 50.247665 33 GCGCGTG 20 4.0388788E-4 50.247665 34 GAGCTAC 20 4.0388788E-4 50.247665 46 TGGAGGC 20 4.0388788E-4 50.247665 35 TTGAGCT 20 4.0388788E-4 50.247665 44 GCTACTT 20 4.0388788E-4 50.247665 48 CGCAGGC 40 2.4683686E-9 50.247665 46 GAACAGG 20 4.0388788E-4 50.247665 49 GCTTGAG 20 4.0388788E-4 50.247665 42 CGAGCGA 20 4.0388788E-4 50.247665 49 TGGGTGC 65 0.0 50.247665 37 AATATTA 20 4.0388788E-4 50.247665 40 CTTGAGC 20 4.0388788E-4 50.247665 43 CGGTCGG 20 4.0388788E-4 50.247665 34 TGGTGCG 20 4.0388788E-4 50.247665 37 GCCGAGC 20 4.0388788E-4 50.247665 47 TGAGCTA 20 4.0388788E-4 50.247665 45 >>END_MODULE