FastQCFastQC Report
Tue 31 May 2016
SRR212562_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212562_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110278
Sequences flagged as poor quality0
Sequence length58
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT21731.9704746186909448No Hit
AGGACTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT13441.2187380982607592No Hit
AGGACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT13301.2060429097372096No Hit
AGGACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC11521.0446326556520793No Hit
AGGACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC11211.0165218810642196No Hit
AGGACTGGGATCCATATTTTCCCTTTTTGTTGAGTATAGGCTTTAATTCTTTGAATTT9450.8569252253395964No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA9240.8378824425542719No Hit
AGGACTGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC8200.7435753278079037No Hit
AGGACTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG7710.6991421679754801No Hit
AGGACTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT7060.640200221259No Hit
AGGACTGGGAGTGCAGTGCTAGGACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAG6920.6275050327354503No Hit
AGGACTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6410.5812582745425199No Hit
AGGACTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC6060.5495203032336459No Hit
AGGACTGGGAACTCTAGCCTGATTGGCTTAGGGTACACTCAGACCCTAAAACCAGGTA5500.49873954913944757No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5220.47334917209234845No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA4810.43617040570195326No Hit
AGGACTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC4070.3690672663631912No Hit
AGGACTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC3890.35274488111862745No Hit
AGGACTGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3770.341863290955585No Hit
AGGACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG3730.3382360942345708No Hit
AGGACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3720.3373292950543173No Hit
AGGACTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3460.31375251636772516No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAGCAGTGG3240.29380293440214733No Hit
AGGACTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2900.26297176227352687No Hit
AGGACTGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2880.2611581639130198No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2810.25481056965124504No Hit
AGGACTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2800.2539037704709915No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCAG2480.22488619670287818No Hit
AGGACTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.19677542211501842No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA2170.19677542211501842No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGGACACTGACTGCTCTTTCAAA2120.1922414262137507No Hit
AGGACTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.17954623769020112No Hit
AGGACTGGGGCTAGGACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGA1950.1768258401494405No Hit
AGGACTGGGTGTCTGGGAATAGATTTTTCTGTGTAGTTCTTTTAGGAATCATAAAGGT1910.17319864342842634No Hit
AGGACTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1800.1632238524456374No Hit
AGGACTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1760.15959665572462323No Hit
AGGACTGGGGTGCTAGGACTGGGACTAACTAACCATTTGCTTACAAATATCCTGCTGG1680.1523422622825949No Hit
AGGACTGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG1680.1523422622825949No Hit
AGGACTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.15143546310234135No Hit
AGGACTGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT1660.1505286639220878No Hit
AGGACTGGGAGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1600.14508786884056657No Hit
AGGACTGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGATCT1540.13964707375904534No Hit
AGGACTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC1540.13964707375904534No Hit
AGGACTGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1490.13511307785777762No Hit
AGGACTGGGAGTATAAATTAGTCTTTAACTACATAAACATTTGAGAAATCACCTAATT1460.13239268031701698No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC1430.12967228277625636Illumina Single End Adapter 2 (96% over 25bp)
AGGACTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA1380.12513828687498865No Hit
AGGACTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1360.12332468851448158No Hit
AGGACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1350.12241788933422804No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA1300.11788389343296035No Hit
AGGACTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG1300.11788389343296035No Hit
AGGACTGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCAA1270.11516349589219971No Hit
AGGACTGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGCA1250.11334989753169264No Hit
AGGACTGGGGCTAGGACTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAG1250.11334989753169264No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA1190.10790910245017139No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAGCAGGGG1180.10700230326991785No Hit
AGGACTGGGGGGAGAGACATGAGTGGACCAGCGGGTCCAACACAGGGAAATCCAAGTC1160.10518870490941075No Hit
AGGACTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1130.10246830736865013No Hit
AGGACTGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGA1130.10246830736865013No Hit
AGGACTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1110.10065470900814306No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCA150.00716222652.07451252
CGATGAT150.00716222652.07451250
GCCAAAT150.00716222652.07451251
AAGCCGT150.00716222652.07451250
CAGCTCG251.6314703E-552.0745150
AGCCCAA700.052.0745152
AGCTCGA251.6314703E-552.0745151
CCTAATT203.4097827E-452.0745152
TCGAACT800.052.0745152
TGATACA203.4097827E-452.0745150
CACCTAA203.4097827E-452.0745150
ACCTAAT203.4097827E-452.0745151
TATGGGT203.4097827E-452.0745152
GAAATCA203.4251183E-452.02723745
AAATCAC203.4251183E-452.02723746
GCGATGA150.00718801152.02723749
CATCCCG150.00718801152.02723743
TTATTAG150.00718801152.02723745
CTGATAC203.4251183E-452.02723749
GATTGGG150.00718801152.02723743
Produced by FastQC (version 0.11.5)