##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212562_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 110278 Sequences flagged as poor quality 0 Sequence length 58 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.602722211139124 39.0 38.0 40.0 33.0 40.0 2 36.81486787935944 39.0 36.0 40.0 31.0 40.0 3 36.79772937485264 39.0 36.0 40.0 31.0 40.0 4 36.43434773934965 38.0 35.0 39.0 31.0 40.0 5 37.12885616351403 39.0 36.0 40.0 33.0 40.0 6 37.15295888572517 39.0 37.0 40.0 33.0 40.0 7 37.07203612687934 39.0 37.0 40.0 32.0 40.0 8 36.67849435064111 39.0 37.0 40.0 30.0 40.0 9 36.492645858648146 39.0 36.0 40.0 29.0 40.0 10 36.5479152686846 39.0 35.0 40.0 30.0 40.0 11 37.15565207929052 39.0 37.0 40.0 33.0 40.0 12 37.01076370626961 39.0 36.0 40.0 32.0 40.0 13 36.92267723389978 39.0 36.0 40.0 32.0 40.0 14 36.91962132066232 39.0 36.0 40.0 32.0 40.0 15 36.899907506483615 39.0 36.0 40.0 32.0 40.0 16 36.711184461089246 38.0 36.0 40.0 32.0 40.0 17 36.66838353978128 38.0 36.0 40.0 31.0 40.0 18 36.5214367326212 38.0 36.0 40.0 31.0 40.0 19 36.26561961587987 38.0 35.0 40.0 30.0 40.0 20 36.27990170296886 38.0 35.0 40.0 30.0 40.0 21 36.16150093400316 38.0 35.0 40.0 30.0 40.0 22 36.035455847947915 38.0 35.0 39.0 30.0 40.0 23 35.821260813580224 38.0 35.0 39.0 30.0 40.0 24 35.538421081267344 38.0 35.0 39.0 29.0 40.0 25 35.631422405194144 38.0 35.0 39.0 29.0 40.0 26 35.33667639964453 38.0 35.0 39.0 29.0 40.0 27 35.234951667603696 38.0 35.0 39.0 28.0 40.0 28 34.71470284190863 38.0 34.0 39.0 27.0 40.0 29 34.79975153702461 38.0 35.0 39.0 27.0 40.0 30 34.348482924971435 38.0 33.0 39.0 26.0 40.0 31 33.75675111989699 38.0 33.0 39.0 23.0 40.0 32 33.175347757485625 38.0 33.0 39.0 20.0 40.0 33 33.13855891474274 38.0 33.0 39.0 20.0 40.0 34 32.6316037650302 37.0 32.0 39.0 17.0 40.0 35 32.428961352218934 37.0 31.0 39.0 17.0 40.0 36 32.513239268031704 38.0 33.0 39.0 8.0 40.0 37 32.34092928779992 38.0 32.0 39.0 2.0 40.0 38 32.08176608208346 38.0 32.0 39.0 2.0 40.0 39 32.073695569379204 38.0 32.0 39.0 2.0 40.0 40 32.01509820635122 38.0 32.0 39.0 2.0 40.0 41 32.22279149059649 38.0 33.0 39.0 2.0 40.0 42 32.002856417417796 38.0 32.0 39.0 2.0 40.0 43 31.772221113912114 38.0 31.0 39.0 2.0 40.0 44 31.699332595803334 37.0 31.0 39.0 2.0 40.0 45 31.60965015687626 37.0 31.0 39.0 2.0 40.0 46 31.06773789876494 37.0 30.0 39.0 2.0 40.0 47 31.13755236765266 37.0 31.0 39.0 2.0 40.0 48 31.07160993126462 37.0 30.0 39.0 2.0 40.0 49 30.848782168700918 36.0 30.0 39.0 2.0 40.0 50 30.65912512015089 36.0 30.0 39.0 2.0 40.0 51 30.533234189956293 36.0 30.0 39.0 2.0 40.0 52 30.365784653330675 36.0 30.0 39.0 2.0 40.0 53 30.18086109650157 36.0 29.0 39.0 2.0 40.0 54 29.868287419068174 36.0 29.0 39.0 2.0 40.0 55 29.782195904894902 36.0 29.0 39.0 2.0 40.0 56 29.21140209289251 36.0 27.0 39.0 2.0 40.0 57 29.020702225285188 36.0 27.0 39.0 2.0 40.0 58 28.8050835162045 35.0 27.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1 1 -2.0906157684415305 1 2 -3.783612181097503 1 3 -3.8109390243022006 1 4 -2.7306468498936667 1 5 -3.457052403052984 1 6 -3.191407939256635 1 7 -3.5808510428947145 1 8 -3.82852651221269 1 9 -4.380586572049417 1 10 -2.502164656159735 1 11 -1.7228414573909347 1 12 -2.007685707881997 1 13 -2.235532489956867 1 14 -2.1573916321170685 1 15 -2.401824652462146 1 16 -3.041734164293608 1 17 -2.929629173418199 1 18 -3.693301026397556 1 19 -2.94244063764323 1 20 -2.480107490870296 1 21 -3.4224043029331312 1 22 -3.102680253804884 1 23 -4.318747520088998 1 24 -3.57350449712267 1 25 -3.965665635685564 1 26 -2.9524129203674576 1 27 -2.9582267398275093 1 28 -3.144399591968579 1 29 -2.9350731610441407 1 30 -2.7311924937230287 1 31 -2.078264299243081 1 32 -2.3317929239853825 1 33 -2.944953170413264 1 34 -4.111762697557253 1 35 -2.6172488114866965 1 36 -3.1537270351271083 1 37 -3.1931288964764235 1 38 -2.7000713910402787 1 39 -2.4431658149625157 1 40 -3.0877255047293275 1 41 -3.123067966633183 1 42 -3.4591643815182103 1 43 -2.4237551994180215 1 44 -2.86026324558852 1 45 -3.4031564457445 1 46 -3.320233283248445 1 47 -1.7299654551660666 1 48 -3.1779250400220356 1 49 -2.8869381111792833 1 50 -2.3994104393853597 1 51 -2.010389662113777 1 52 -2.3383820191734443 1 53 -2.189171168376692 1 54 -2.49977661699425 1 55 -1.519625322452704 1 56 -1.6124069842469382 1 57 -2.1195350496323115 1 58 -1.8961351866113851 2 1 -0.48322316104892593 2 2 -1.043352440837765 2 3 -0.6640858774490539 2 4 -0.20617132541813987 2 5 -0.42808137408195535 2 6 -0.551048298896994 2 7 -1.6857461477898141 2 8 -1.8774775611637438 2 9 -1.777189968652813 2 10 -0.6979688519639353 2 11 -0.2353289698784451 2 12 -0.005687705884000138 2 13 -0.8519161063404823 2 14 -0.3661828409082801 2 15 -0.13808838872588325 2 16 -0.5642116867711309 2 17 -0.042516286305314566 2 18 -0.11188244497897415 2 19 -0.24513794034053404 2 20 -0.30528231604512257 2 21 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-1.9402677602536151 117 50 -1.7443952418169708 117 51 -1.3704338732218169 117 52 -1.482758918869493 117 53 -1.4198978884651154 117 54 -1.7441811486328227 117 55 -1.609290975948145 117 56 -1.8305611704862308 117 57 -1.937855027526755 117 58 -2.4165828240802973 118 1 -0.4221990787482497 118 2 -1.1512551764000776 118 3 -1.210496913221796 118 4 -0.3823462143589893 118 5 -0.9587103196045206 118 6 -0.5153924653688193 118 7 -1.5164686168101582 118 8 -1.3594742878413157 118 9 -1.7989341818863878 118 10 -0.9253478559386821 118 11 -0.8169282216904605 118 12 -0.8883157161715829 118 13 -0.24658526696709515 118 14 -0.2652943676793811 118 15 -0.8942258682676183 118 16 -0.1840386683002393 118 17 0.39739486637179766 118 18 0.11023586224571602 118 19 0.11586276108570104 118 20 0.27023790841127493 118 21 0.07607594022795894 118 22 0.29204204517273524 118 23 0.1783511259458166 118 24 0.44652866120836165 118 25 0.7892390341127253 118 26 0.7454317881155461 118 27 0.3660341057099288 118 28 0.6707427125354286 118 29 0.4311882371321474 118 30 -0.11582913367882242 118 31 0.5136119096544078 118 32 0.18934551204667116 118 33 0.16516012055109286 118 34 0.6157863491407269 118 35 1.5290623241861425 118 36 1.0583481237565593 118 37 1.065920564700697 118 38 0.4811941519273901 118 39 0.6745462601963652 118 40 0.714706106212919 118 41 0.9147877946268324 118 42 0.9279591332648813 118 43 0.701831979360648 118 44 1.0236825958041287 118 45 0.4646247092706979 118 46 0.9016512152317979 118 47 1.3992138761409265 118 48 0.9600965128931342 118 49 0.9852641546400029 118 50 1.4045409283957966 118 51 0.4806299565654193 118 52 0.6555389534709306 118 53 0.9843574306838221 118 54 0.2132656598778162 118 55 0.4439005134135563 118 56 0.7119920210031303 118 57 0.4557619937498387 118 58 1.3706512184728936 119 1 -0.8011352489610104 119 2 -0.9776381551234792 119 3 -0.6649649983281805 119 4 -0.6229845122313264 119 5 -0.21445500045557964 119 6 -0.8204988483475404 119 7 -1.4783835104271787 119 8 -1.0567083303945068 119 9 -1.2685086499714942 119 10 -0.6991776431727246 119 11 -1.8354388599883364 119 12 -1.4846986948949876 119 13 -0.9948831393075181 119 14 -1.2116773464027872 119 15 -1.0303960810335795 119 16 -1.2021237746832156 119 17 -1.3619668357558652 119 18 -1.4455088186053473 119 19 -0.9671159623185588 119 20 -0.472315283078089 119 21 -0.8166900172188463 119 22 -0.3777451888698238 119 23 -0.07420206554354536 119 24 -0.6615564451746181 119 25 0.004983714963785246 119 26 -0.7890362969908367 119 27 -0.42290206450283563 119 28 -0.5447892023581886 119 29 -0.8022160181869964 119 30 -0.7285950911256265 119 31 -0.3855370265158058 119 32 -0.874909807102263 119 33 0.3392026737425766 119 34 -0.6082562040507611 119 35 0.233530409292527 119 36 -0.09931145071152514 119 37 -0.3289730523205776 119 38 0.5592792583103687 119 39 0.3724186006218986 119 40 0.855131638127812 119 41 1.2509580073927893 119 42 0.3743421119882875 119 43 1.4694915538287319 119 44 1.451554936229666 119 45 1.4339864113983545 119 46 2.0054810024658387 119 47 1.7743202591196479 119 48 1.6058411937441939 119 49 0.6799450057038321 119 50 1.3877324177574977 119 51 2.1970129352888215 119 52 1.6344751236836963 119 53 1.1356340264285016 119 54 1.6464571492395166 119 55 0.9075175346901538 119 56 1.0660345741946209 119 57 1.5391662490689875 119 58 2.012566112089914 120 1 -2.8000509116116135 120 2 -3.5648670707861285 120 3 -3.2186999380872123 120 4 -2.4411772833156675 120 5 -3.2592742775640176 120 6 -2.751101257986093 120 7 -3.5182630284994687 120 8 -3.4177926677439103 120 9 -3.7476652764775125 120 10 -3.151828245582365 120 11 -3.2218244021570115 120 12 -3.344096285256434 120 13 -2.969220488705112 120 14 -3.2090267439931424 120 15 -3.0231671653709284 120 16 -4.0617623289000875 120 17 -4.049798161057069 120 18 -4.124063035472815 120 19 -4.592417167137832 120 20 -4.296411668620255 120 21 -4.461991222038122 120 22 -4.290757237062593 120 23 -3.8882984510857135 120 24 -4.1601106620420865 120 25 -4.735498212747057 120 26 -4.136265212653488 120 27 -3.572058691008859 120 28 -3.5138253469365033 120 29 -3.250047343488202 120 30 -3.6538962959449037 120 31 -3.4561394361543627 120 32 -2.9137049878251595 120 33 -2.7273033503538073 120 34 -2.967653794412204 120 35 -2.3723732051653066 120 36 -2.641600607338031 120 37 -1.7827079920796152 120 38 -1.9181906212077031 120 39 -2.099870556004607 120 40 -2.041253904040861 120 41 -2.474343197426485 120 42 -1.845175960300871 120 43 -2.104243385930303 120 44 -1.3772402444932297 120 45 -2.2448087693245355 120 46 -2.0345189975341604 120 47 -1.8349568493140858 120 48 -1.8357250713160447 120 49 -1.7098140304407465 120 50 -2.229014570194309 120 51 -2.2563605586870814 120 52 -1.7617899365572676 120 53 -1.7654503109208939 120 54 -1.3480006820857824 120 55 -1.795012585791774 120 56 -2.066013618576463 120 57 -2.411195196714143 120 58 -2.2962290686329787 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 214.0 3 25.0 4 79.0 5 38.0 6 72.0 7 165.0 8 152.0 9 227.0 10 341.0 11 348.0 12 457.0 13 544.0 14 689.0 15 720.0 16 799.0 17 851.0 18 1006.0 19 975.0 20 1040.0 21 1015.0 22 1286.0 23 1240.0 24 1392.0 25 1113.0 26 1132.0 27 1291.0 28 1627.0 29 1991.0 30 2443.0 31 2992.0 32 3767.0 33 4561.0 34 6014.0 35 7716.0 36 10304.0 37 14776.0 38 19190.0 39 17656.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 45.32998422169426 31.86673679246994 5.146992147119099 17.6562868387167 11 28.09626580097572 25.990678104426994 17.859409855093492 28.0536462395038 12 37.245869529733945 12.74778287600428 30.77585737862493 19.230490215636845 13 17.88207983459983 21.237236801537932 30.716008632728197 30.164674731134046 14 21.410435444966357 26.659895899454106 36.665518054371674 15.264150601207858 15 33.084568091550445 14.816191806162607 36.43519106258728 15.664049039699668 16 21.855872031339263 20.36961469753439 34.409713720902815 23.364799550223527 17 30.272583833584214 13.969241371805799 22.16126516621629 33.596909628393696 18 32.12880175556322 15.717550191334627 28.573242169789076 23.580405883313084 19 27.961659835322283 17.865174652689614 33.81606151837209 20.35710399361602 20 30.942017157262818 25.48016758256706 24.782813718555598 18.79500154161452 21 32.43495687974391 17.617186437296528 30.34287631605198 19.604980366907583 22 31.873373414220552 17.407981718762752 22.619403864813155 28.09924100220354 23 31.89814646885995 28.82585512713555 20.606478290833905 18.66952011317059 24 28.30755000997479 30.858376104028 20.856381145831442 19.97769274016576 25 25.609822448720504 20.237944104898528 22.469576887502495 31.68265655887847 26 31.9187723339803 18.01864717299425 25.550073464057032 24.512507028968418 27 23.76919100777162 18.820563510560156 24.669683422052543 32.74056205961568 28 22.006857018466786 28.865689511301383 25.846243152051663 23.281210318180168 29 31.538231319662984 18.330718373343732 28.294169394991975 21.836880912001305 30 22.861419823089136 24.343842141075072 30.840553413472442 21.95418462236335 31 34.74912231364474 16.099534622118604 30.29129208131866 18.860050982918 32 32.74142059058261 13.68443009504462 37.17532467532468 16.398824639048105 33 32.66675735727566 13.754137759035052 34.3082573799483 19.27084750374099 34 23.848177036096498 17.16941773988754 40.692907672773444 18.28949755124252 35 22.165588920833297 30.04960956276471 34.38296405800782 13.401837458394175 36 28.72356831190979 25.697361140089082 30.305614267441968 15.273456280559158 37 23.85514188257493 22.45808839538428 37.20607446113651 16.480695260904273 38 25.567805453473074 21.72768930629282 36.13810625652194 16.566398983712173 39 23.70295107546879 22.246917836180387 34.70620787255854 19.34392321579229 40 24.65477508211 18.66029142245368 38.673356439057144 18.011577056379178 41 21.863942368760096 22.596127674245587 37.406775662777406 18.133154294216915 42 26.901715219166896 18.942735275433343 36.782829657863694 17.372719847536075 43 24.36312813671304 16.198825632787898 38.931089874486105 20.50695635601296 44 20.868302862586763 20.2540489044141 38.243433289479654 20.634214943519485 45 26.153776366523022 18.24268541619415 35.5110002268088 20.092537990474028 46 19.765732742977427 21.39072366988459 39.369057124192494 19.47448646294549 47 25.514668506281318 19.62185026504974 39.40164112991068 15.461840098758259 48 25.9650205574565 19.162453825139092 37.175868359669266 17.69665725773514 49 22.50283691162453 20.894194544051565 36.5457764059734 20.057192138350505 50 17.200493880960163 19.81878926535207 40.82325598285942 22.15746087082834 51 17.750850918992512 21.913319718629452 42.35262083049693 17.983208531881097 52 17.478468421864648 19.941553903818054 42.70376723206912 19.87621044224818 53 16.044193478102986 20.018701430750234 43.11042922507081 20.826675866075966 54 17.311131892176533 22.273771369946342 41.04663936882053 19.368457369056593 55 19.096011333914557 21.083079046788725 35.24320691659401 24.5777027027027 56 19.06500631261524 20.730810103817543 38.29895455825318 21.90522902531404 57 20.386533099621275 22.18569209950321 36.91681727773893 20.510957523136586 58 20.265949716610958 22.94815433803226 37.45095189652667 19.33494404883011 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 43.5 10 87.0 11 136.0 12 185.0 13 207.0 14 229.0 15 272.5 16 628.5 17 941.0 18 1096.5 19 1252.0 20 1240.5 21 1229.0 22 1357.5 23 1760.5 24 2035.0 25 2390.0 26 2745.0 27 3022.5 28 3438.5 29 3577.0 30 3319.0 31 3061.0 32 3189.0 33 3317.0 34 3601.0 35 2774.5 36 1664.0 37 1681.0 38 1698.0 39 1547.5 40 1397.0 41 1444.5 42 1610.5 43 1729.0 44 2005.0 45 2281.0 46 2919.5 47 2894.0 48 2230.0 49 2570.5 50 2911.0 51 2839.5 52 2768.0 53 4135.5 54 5426.0 55 5349.0 56 5220.5 57 5092.0 58 5658.5 59 7150.0 60 8075.0 61 8560.0 62 9045.0 63 8668.5 64 8292.0 65 7237.5 66 4663.0 67 3143.0 68 2490.5 69 1838.0 70 1390.0 71 942.0 72 766.5 73 487.0 74 383.0 75 255.0 76 127.0 77 85.0 78 35.0 79 27.0 80 23.0 81 19.0 82 17.0 83 15.0 84 12.0 85 6.5 86 4.0 87 2.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 9.067991802535411E-4 17 0.0 18 0.0 19 0.0018135983605070821 20 0.0036271967210141642 21 0.004533995901267705 22 9.067991802535411E-4 23 0.0018135983605070821 24 0.0 25 0.0 26 0.01813598360507082 27 0.004533995901267705 28 0.023576778686592068 29 0.013601987703803114 30 0.048060356553437676 31 0.04080596311140935 32 0.012695188523549577 33 0.011788389343296034 34 0.01632238524456374 35 0.015415586064310198 36 0.03717876639039518 37 0.04171276229166289 38 0.06619634015850849 39 0.04261956147191643 40 0.05440795081521246 41 0.05440795081521246 42 0.07979832786231161 43 0.08251872540307223 44 0.05712834835597309 45 0.048060356553437676 46 0.056221549175719546 47 0.09974790982788952 48 0.08977311884510056 49 0.11153629917118556 50 0.11788389343296035 51 0.09340031556611472 52 0.08251872540307223 53 0.11425669671194617 54 0.12241788933422804 55 0.1505286639220878 56 0.1641306516258909 57 0.15506265982335551 58 0.16503745080614446 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 110278.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.680135657157365 #Duplication Level Percentage of deduplicated Percentage of total 1 71.51141818585421 23.37002847349426 2 11.021393490385416 7.20361268793413 3 5.177724132190127 5.076261811059323 4 2.8968617331224507 3.7867933767387876 5 1.8313493715141929 2.9924372948366855 6 1.2847193318349566 2.5190881227443374 7 0.8574044784816449 1.9614066268884092 8 0.8657287938067094 2.2633707539128385 9 0.621548877604817 1.8281071473911388 >10 3.460140403451816 20.373963981936562 >50 0.28857626460223645 6.602404831426033 >100 0.14151336052609673 9.058017011552622 >500 0.027747717750215044 6.508097716679664 >1k 0.013873858875107522 6.456410163405213 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGGACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 2173 1.9704746186909448 No Hit AGGACTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 1344 1.2187380982607592 No Hit AGGACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 1330 1.2060429097372096 No Hit AGGACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 1152 1.0446326556520793 No Hit AGGACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 1121 1.0165218810642196 No Hit AGGACTGGGATCCATATTTTCCCTTTTTGTTGAGTATAGGCTTTAATTCTTTGAATTT 945 0.8569252253395964 No Hit AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 924 0.8378824425542719 No Hit AGGACTGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC 820 0.7435753278079037 No Hit AGGACTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 771 0.6991421679754801 No Hit AGGACTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT 706 0.640200221259 No Hit AGGACTGGGAGTGCAGTGCTAGGACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAG 692 0.6275050327354503 No Hit AGGACTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 641 0.5812582745425199 No Hit AGGACTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 606 0.5495203032336459 No Hit AGGACTGGGAACTCTAGCCTGATTGGCTTAGGGTACACTCAGACCCTAAAACCAGGTA 550 0.49873954913944757 No Hit AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 522 0.47334917209234845 No Hit AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA 481 0.43617040570195326 No Hit AGGACTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC 407 0.3690672663631912 No Hit AGGACTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 389 0.35274488111862745 No Hit AGGACTGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 377 0.341863290955585 No Hit AGGACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG 373 0.3382360942345708 No Hit AGGACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 372 0.3373292950543173 No Hit AGGACTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 346 0.31375251636772516 No Hit AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAGCAGTGG 324 0.29380293440214733 No Hit AGGACTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 290 0.26297176227352687 No Hit AGGACTGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 288 0.2611581639130198 No Hit AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 281 0.25481056965124504 No Hit AGGACTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 280 0.2539037704709915 No Hit AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCAG 248 0.22488619670287818 No Hit AGGACTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 217 0.19677542211501842 No Hit AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 217 0.19677542211501842 No Hit AGGACTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGGACACTGACTGCTCTTTCAAA 212 0.1922414262137507 No Hit AGGACTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 198 0.17954623769020112 No Hit AGGACTGGGGCTAGGACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGA 195 0.1768258401494405 No Hit AGGACTGGGTGTCTGGGAATAGATTTTTCTGTGTAGTTCTTTTAGGAATCATAAAGGT 191 0.17319864342842634 No Hit AGGACTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT 180 0.1632238524456374 No Hit AGGACTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 176 0.15959665572462323 No Hit AGGACTGGGGTGCTAGGACTGGGACTAACTAACCATTTGCTTACAAATATCCTGCTGG 168 0.1523422622825949 No Hit AGGACTGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG 168 0.1523422622825949 No Hit AGGACTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 167 0.15143546310234135 No Hit AGGACTGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT 166 0.1505286639220878 No Hit AGGACTGGGAGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 160 0.14508786884056657 No Hit AGGACTGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGATCT 154 0.13964707375904534 No Hit AGGACTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC 154 0.13964707375904534 No Hit AGGACTGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT 149 0.13511307785777762 No Hit AGGACTGGGAGTATAAATTAGTCTTTAACTACATAAACATTTGAGAAATCACCTAATT 146 0.13239268031701698 No Hit AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC 143 0.12967228277625636 Illumina Single End Adapter 2 (96% over 25bp) AGGACTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA 138 0.12513828687498865 No Hit AGGACTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA 136 0.12332468851448158 No Hit AGGACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC 135 0.12241788933422804 No Hit AGGACTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA 130 0.11788389343296035 No Hit AGGACTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG 130 0.11788389343296035 No Hit AGGACTGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCAA 127 0.11516349589219971 No Hit AGGACTGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGCA 125 0.11334989753169264 No Hit AGGACTGGGGCTAGGACTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAG 125 0.11334989753169264 No Hit AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA 119 0.10790910245017139 No Hit AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAGCAGGGG 118 0.10700230326991785 No Hit AGGACTGGGGGGAGAGACATGAGTGGACCAGCGGGTCCAACACAGGGAAATCCAAGTC 116 0.10518870490941075 No Hit AGGACTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 113 0.10246830736865013 No Hit AGGACTGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAGA 113 0.10246830736865013 No Hit AGGACTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT 111 0.10065470900814306 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.019949581965577903 0.0 0.0 0.0 0.0 11 0.04352636065216997 0.0 0.0 0.0 0.0 12 0.04896715573369122 0.0 0.0 0.0 0.0 13 0.05168755327445184 0.0 0.0 0.0 0.0 14 0.06891673769926912 0.0 0.0 0.0 0.0 15 0.0707303360597762 0.0 0.0 0.0 0.0 16 0.0707303360597762 0.0 0.0 0.0 0.0 17 0.07163713524002974 0.0 0.0 0.0 0.0 18 0.07163713524002974 0.0 0.0 0.0 0.0 19 0.07345073360053682 0.0 0.0 0.0 0.0 20 0.09884111064763597 0.0 0.0 0.0 0.0 21 0.10065470900814305 0.0 0.0 0.0 0.0 22 0.10428190572915721 0.0 0.0 0.0 0.0 23 0.11153629917118554 0.0 0.0 0.0 0.0 24 0.1260450860552422 0.0 0.0 0.0 0.0 25 0.1305790819565099 0.0 0.0 0.0 0.0 26 0.13148588113676346 0.0 0.0 0.0 0.0 27 0.13329947949727053 0.0 0.0 0.0 0.0 28 0.21309780735958214 0.0 0.0 0.0 0.0 29 0.2185386024411034 0.0 0.0 0.0 0.0 30 0.25753096719200563 0.0 0.0 0.0 0.0 31 0.2602513647327663 0.0 0.0 0.0 0.0 32 0.26659895899454106 0.0 0.0 0.0 0.0 33 0.2675057581747946 0.0 0.0 0.0 0.0 34 0.2720397540760623 0.0 0.0 0.0 0.0 35 0.3708808647236983 0.0 0.0 0.0 0.0 36 0.375414860624966 0.0 0.0 0.0 0.0 37 0.3790420573459802 0.0 0.0 0.0 0.0 38 0.3790420573459802 0.0 0.0 0.0 0.0 39 0.3862964507880085 0.0 0.0 0.0 0.0 40 0.39173724586952974 0.0 0.0 0.0 0.0 41 0.39717804095105097 0.0 0.0 0.0 0.0 42 0.40080523767206516 0.0 0.0 0.0 0.0 43 0.4062460327535864 0.0 0.0 0.0 0.0 44 0.40987322947460053 0.0 0.0 0.0 0.0 45 0.4135004261956147 0.0 0.0 0.0 0.0 46 0.4180344220968824 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGCA 15 0.007162226 52.074512 52 CGATGAT 15 0.007162226 52.074512 50 GCCAAAT 15 0.007162226 52.074512 51 AAGCCGT 15 0.007162226 52.074512 50 CAGCTCG 25 1.6314703E-5 52.07451 50 AGCCCAA 70 0.0 52.07451 52 AGCTCGA 25 1.6314703E-5 52.07451 51 CCTAATT 20 3.4097827E-4 52.07451 52 TCGAACT 80 0.0 52.07451 52 TGATACA 20 3.4097827E-4 52.07451 50 CACCTAA 20 3.4097827E-4 52.07451 50 ACCTAAT 20 3.4097827E-4 52.07451 51 TATGGGT 20 3.4097827E-4 52.07451 52 GAAATCA 20 3.4251183E-4 52.027237 45 AAATCAC 20 3.4251183E-4 52.027237 46 GCGATGA 15 0.007188011 52.027237 49 CATCCCG 15 0.007188011 52.027237 43 TTATTAG 15 0.007188011 52.027237 45 CTGATAC 20 3.4251183E-4 52.027237 49 GATTGGG 15 0.007188011 52.027237 43 >>END_MODULE