FastQCFastQC Report
Tue 31 May 2016
SRR212561_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212561_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109251
Sequences flagged as poor quality0
Sequence length58
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT20091.8388847699334558No Hit
AGGACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT13311.2182954847095222No Hit
AGGACTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT13151.2036503098369808No Hit
AGGACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC11411.0443840330980951No Hit
AGGACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC10580.9684121884467876No Hit
AGGACTGGGATCCATATTTTCCCTTTTTGTTGAGTATAGGCTTTAATTCTTTGAATTT8930.8173838225737063No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA8800.8054846179897667No Hit
AGGACTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG7150.6544562521166853No Hit
AGGACTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT7090.6489643115394824No Hit
AGGACTGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC6790.6215046086534677No Hit
AGGACTGGGAGTGCAGTGCTAGGACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAG6650.6086900806399942No Hit
AGGACTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5890.5391254999954234No Hit
AGGACTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5570.509835150250341No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA5000.45766171476691286No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG4860.4448471867534393No Hit
AGGACTGGGAACTCTAGCCTGATTGGCTTAGGGTACACTCAGACCCTAAAACCAGGTA4830.44210121646483785No Hit
AGGACTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC4310.3945043981290789No Hit
AGGACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG3990.3652140483839965No Hit
AGGACTGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC3710.33958499235704936No Hit
AGGACTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC3620.3313470814912449No Hit
AGGACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3470.31761723004823755No Hit
AGGACTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3220.2947341443098919No Hit
AGGACTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3160.28924220373268894No Hit
AGGACTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2750.25171394312180206No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2730.24988329626273445No Hit
AGGACTGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC2660.24347603225599768No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCAG2660.24347603225599768No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGGACACTGACTGCTCTTTCAAA2470.22608488709485497No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAGCAGTGG2370.21693165279951673No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA2350.21510100594044906No Hit
AGGACTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.183980009336299No Hit
AGGACTGGGTGTCTGGGAATAGATTTTTCTGTGTAGTTCTTTTAGGAATCATAAAGGT1930.17665742190002837No Hit
AGGACTGGGGCTAGGACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGA1910.17482677504096072No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC1840.16841951103422392Illumina Single End Adapter 2 (96% over 25bp)
AGGACTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.16750418760469013No Hit
AGGACTGGGAGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1720.15743562987981802No Hit
AGGACTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.15468965959121656No Hit
AGGACTGGGGTGCTAGGACTGGGACTAACTAACCATTTGCTTACAAATATCCTGCTGG1620.14828239558447978No Hit
AGGACTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT1600.1464517487254121No Hit
AGGACTGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG1580.14462110186634447No Hit
AGGACTGGGAGTATAAATTAGTCTTTAACTACATAAACATTTGAGAAATCACCTAATT1480.1354678675710062No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA1470.13455254414147239No Hit
AGGACTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA1400.1281452801347356No Hit
AGGACTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1380.12631463327566794No Hit
AGGACTGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT1300.11899204583939735No Hit
AGGACTGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGATCT1290.11807672240986351No Hit
AGGACTGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1290.11807672240986351No Hit
AGGACTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC1290.11807672240986351No Hit
AGGACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1230.11258478183266056No Hit
AGGACTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1220.11166945840312675No Hit
AGGACTGGGGGGCTGGAGAGATGGCTTGGCGGTTAAGAGCACTGGCTGCTCTTCCGAA1210.1107541349735929No Hit
AGGACTGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCAA1160.10617751782592379No Hit
AGGACTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG1160.10617751782592379No Hit
AGGACTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1160.10617751782592379No Hit
AGGACTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1150.10526219439638995No Hit
AGGACTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC1140.10434687096685615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAGG458.913048E-1152.15540726
TAGCGCG150.00711817952.15540323
AACGCGG203.3835793E-452.15540323
AACGCGA203.3835793E-452.15540323
TAACTAC251.6164244E-552.15540326
ACTTACC251.6164244E-552.15540325
AGTAAGC150.00711817952.15540326
AGACGAA150.00711817952.15540326
CTTACCC251.6164244E-552.15540326
CGGCTTA150.00711817952.15540325
TTAACTA251.6164244E-552.15540325
ACGCGGA150.00711817952.15540324
ACGCGAT150.00711817952.15540324
CTTCCTA150.00711817952.15540327
TTTAACT251.6164244E-552.15540324
TTGCCGG150.00711817952.15540326
ACCAGCG251.6164244E-552.15540327
GCTTACA150.00711817952.15540327
CGCGATC150.00711817952.15540325
CTTTTTG1050.052.15540323
Produced by FastQC (version 0.11.5)