##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR212561_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	109251
Sequences flagged as poor quality	0
Sequence length	58
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.59342248583537	39.0	38.0	40.0	33.0	40.0
2	36.87923222670731	39.0	36.0	40.0	32.0	40.0
3	36.864239228931545	39.0	36.0	40.0	31.0	40.0
4	36.439034882975896	38.0	35.0	39.0	31.0	40.0
5	37.14719316070333	39.0	36.0	40.0	33.0	40.0
6	37.17480846857237	39.0	37.0	40.0	33.0	40.0
7	37.18794335978618	39.0	38.0	40.0	33.0	40.0
8	36.8323127477094	39.0	37.0	40.0	31.0	40.0
9	36.66632799699774	39.0	37.0	40.0	30.0	40.0
10	36.54460828733833	39.0	35.0	40.0	30.0	40.0
11	37.18676259256208	39.0	37.0	40.0	33.0	40.0
12	37.03330861960074	39.0	36.0	40.0	32.0	40.0
13	36.90451345983103	39.0	36.0	40.0	31.0	40.0
14	36.96267311054361	39.0	36.0	40.0	32.0	40.0
15	36.94331402000897	39.0	36.0	40.0	32.0	40.0
16	36.69612177462906	38.0	36.0	40.0	31.0	40.0
17	36.671307356454406	38.0	36.0	40.0	31.0	40.0
18	36.52196318569166	38.0	36.0	40.0	31.0	40.0
19	36.28670675783288	38.0	35.0	40.0	31.0	40.0
20	36.30655096978517	38.0	35.0	40.0	31.0	40.0
21	36.1686666483602	38.0	35.0	40.0	30.0	40.0
22	36.045692945602326	38.0	35.0	39.0	30.0	40.0
23	35.83751178478916	38.0	35.0	39.0	30.0	40.0
24	35.54105683243174	38.0	35.0	39.0	29.0	40.0
25	35.6249828376857	38.0	35.0	39.0	29.0	40.0
26	35.1888769896843	38.0	35.0	39.0	28.0	40.0
27	34.694428426284425	38.0	35.0	39.0	27.0	40.0
28	34.57298331365388	38.0	33.0	39.0	27.0	40.0
29	34.60111120264345	38.0	34.0	39.0	27.0	40.0
30	34.194259091449965	38.0	33.0	39.0	26.0	40.0
31	33.578164044265044	38.0	33.0	39.0	23.0	40.0
32	32.97448993601889	37.0	32.0	39.0	19.0	40.0
33	32.9630850060869	37.0	32.0	39.0	19.0	40.0
34	32.426302734071086	36.0	31.0	39.0	17.0	40.0
35	32.16226853758776	36.0	31.0	39.0	17.0	40.0
36	32.36860074507327	38.0	32.0	39.0	8.0	40.0
37	32.16214039230762	37.0	32.0	39.0	2.0	40.0
38	31.924815333498092	37.0	31.0	39.0	2.0	40.0
39	31.908980238167157	37.0	31.0	39.0	2.0	40.0
40	31.85148877355814	37.0	31.0	39.0	2.0	40.0
41	32.05388509029665	38.0	32.0	39.0	2.0	40.0
42	31.83353928110498	38.0	31.0	39.0	2.0	40.0
43	31.5839397351054	37.0	31.0	39.0	2.0	40.0
44	31.48771178295851	37.0	31.0	39.0	2.0	40.0
45	31.40869191128685	37.0	31.0	39.0	2.0	40.0
46	30.76233627152154	36.0	30.0	39.0	2.0	40.0
47	30.829713229169528	36.0	30.0	39.0	2.0	40.0
48	30.770473496810098	36.0	30.0	39.0	2.0	40.0
49	30.558109307923957	36.0	30.0	39.0	2.0	40.0
50	30.36605614593917	36.0	29.0	39.0	2.0	40.0
51	30.217151330422606	36.0	30.0	39.0	2.0	40.0
52	30.043441249965674	36.0	29.0	39.0	2.0	40.0
53	29.83968110131715	36.0	29.0	39.0	2.0	40.0
54	29.51115321598887	36.0	28.0	39.0	2.0	40.0
55	29.425359950938663	36.0	28.0	39.0	2.0	40.0
56	28.779397900248053	36.0	26.0	39.0	2.0	40.0
57	28.56251201362001	35.0	26.0	39.0	2.0	40.0
58	28.299264995286084	35.0	24.0	39.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-1.1967429902052018
1	2	-1.847656670620104
1	3	-2.1911648018232626
1	4	-1.95971693684875
1	5	-2.645303864509067
1	6	-2.49893631181439
1	7	-2.5881731694328565
1	8	-2.88726925406921
1	9	-3.1087732875209113
1	10	-2.9347657064283226
1	11	-2.4908549186967264
1	12	-2.7061803568112666
1	13	-2.857456754392473
1	14	-2.8625734754822716
1	15	-2.6182843266086664
1	16	-2.311113023611007
1	17	-2.3664464485135355
1	18	-2.0268363651612376
1	19	-2.1355890502593056
1	20	-2.013937948940878
1	21	-1.4504817131200909
1	22	-2.5279379699613926
1	23	-1.5595319944103423
1	24	-1.4504463550844306
1	25	-2.399909082767266
1	26	-1.9473331927651358
1	27	-1.6600160713860248
1	28	-1.9863565310485427
1	29	-2.136064572716407
1	30	-2.057928826716754
1	31	-1.5481234874858885
1	32	-1.498985470819786
1	33	-1.5819623095322939
1	34	-1.1725966450106817
1	35	-2.0234329698633715
1	36	-2.0722805613802535
1	37	-2.0725952695426386
1	38	-1.9322519833687153
1	39	-2.07536130711231
1	40	-1.7667431900797332
1	41	-1.7607986001478046
1	42	-3.147054671096935
1	43	-1.9533219887773825
1	44	-2.1857565579996816
1	45	-1.9697019227840933
1	46	-2.1120888021561584
1	47	-2.3332659272004648
1	48	-2.372688233415815
1	49	-1.810623043516621
1	50	-2.2209058969726136
1	51	-2.2863631091668957
1	52	-2.4719799496909225
1	53	-1.7193818736243855
1	54	-2.190362500507131
1	55	-1.8509912742659154
1	56	-1.8663427228548954
1	57	-1.915152675762343
1	58	-1.2925306785684398
2	1	-1.0894906919416698
2	2	-2.240574614099849
2	3	-2.064050966844256
2	4	-1.7122989366217567
2	5	-1.6088721314481162
2	6	-1.72535783037074
2	7	-2.0759725111647853
2	8	-2.5785642262630617
2	9	-2.311474456514219
2	10	-2.023064423940518
2	11	-1.3031350231116647
2	12	-1.9613159827334101
2	13	-1.2772728933097355
2	14	-1.3330081594001015
2	15	-1.4537173081090629
2	16	-1.1219176995842162
2	17	-1.5565497285044572
2	18	-1.718812304328182
2	19	-1.7418766453109455
2	20	-1.411622661232336
2	21	-2.2032907018841357
2	22	-1.5286189369344925
2	23	-1.7338595395244099
2	24	-1.6807266864883559
2	25	-2.2262625046263054
2	26	-1.579611027290163
2	27	-1.2707299517628243
2	28	-1.9422071722924414
2	29	-2.668694240177537
2	30	-2.8584055035979254
2	31	-2.7804467198091203
2	32	-3.774436611439466
2	33	-3.0716910576880103
2	34	-3.241260814798448
2	35	-2.673415945689044
2	36	-2.7235119766566136
2	37	-3.447467588235181
2	38	-3.0275873596029683
2	39	-2.712632899440081
2	40	-2.62986882848627
2	41	-2.8227665947000666
2	42	-2.8290430946583918
2	43	-3.1369560825238345
2	44	-2.021076045004559
2	45	-2.417891685580596
2	46	-1.8759067569853478
2	47	-1.752401099144624
2	48	-2.0890654891189158
2	49	-1.9696288317358928
2	50	-1.6217684551385432
2	51	-1.812637084879075
2	52	-1.5691312378534477
2	53	-2.3794658595510683
2	54	-2.5880472127985854
2	55	-3.0053437881689895
2	56	-2.6933771116870346
2	57	-2.6385665901874944
2	58	-3.1162737270305882
3	1	-0.8594350333145826
3	2	-0.723258596783829
3	3	-0.5347786518576569
3	4	-0.2143603672546206
3	5	-0.4601399112873281
3	6	-0.6180603659304182
3	7	-0.5541213464564763
3	8	-0.30047723389035497
3	9	-0.5930658809627829
3	10	-0.33341280571747234
3	11	-0.08957080954744612
3	12	-0.3789618289506862
3	13	0.21901653155111944
3	14	-0.4688564381055187
3	15	-0.3462137006054533
3	16	-0.8809364586029815
3	17	-1.0341832061379321
3	18	-0.924336938424247
3	19	-1.3786525678011543
3	20	-0.5550982332793382
3	21	-0.7645151916800543
3	22	-0.5632509715665321
3	23	-0.018027752264046626
3	24	-0.9567522482281987
3	25	-1.3338691836615553
3	26	-0.3477000810934996
3	27	0.31636549247547663
3	28	-0.3839717569141712
3	29	-0.3009350152754564
3	30	0.07449492930250301
3	31	0.8600397778202336
3	32	0.23731354316782927
3	33	0.6309475735220502
3	34	1.5353831761312549
3	35	0.3519396510951225
3	36	0.24175291717970993
3	37	0.25290346927873486
3	38	0.5422951388509567
3	39	-0.06802354054500981
3	40	1.0250476835730993
3	41	0.9951060088868218
3	42	1.0992191193181107
3	43	0.34706783007150577
3	44	0.7384580716724116
3	45	0.2646479969590878
3	46	0.7138685470756698
3	47	1.2683162787156128
3	48	1.0392585677765709
3	49	0.27032169392891703
3	50	0.8758608996336683
3	51	1.191279633323358
3	52	1.3641814811735564
3	53	0.8090519718239051
3	54	1.8194976233176803
3	55	1.835204552379352
3	56	1.429690297094659
3	57	1.5230495714286683
3	58	1.3442498763501582
4	1	-0.16241776486874215
4	2	-0.4869948605200918
4	3	0.144185241391952
4	4	-0.7520369763597969
4	5	-0.6241901467661322
4	6	-1.031718136731044
4	7	-0.008595443787712043
4	8	-0.3036169513157887
4	9	-0.1550753001150582
4	10	-0.6418900427661427
4	11	-0.01264773262437302
4	12	0.26468024326281636
4	13	0.3171327010958649
4	14	0.2768264504345126
4	15	0.16477531038355409
4	16	0.8553272776607557
4	17	-0.08912826108299043
4	18	0.5434809579650803
4	19	0.26655936312506157
4	20	0.857774608164938
4	21	0.7967092981158643
4	22	0.24993584162028526
4	23	0.8179220122571422
4	24	0.33681133104965966
4	25	-0.15333543169923303
4	26	-1.5046859523650866
4	27	-1.2456439266767987
4	28	-1.8690580991433663
4	29	-1.9948125662958631
4	30	-2.513417158609581
4	31	-2.658013597375998
4	32	-2.3105671475700014
4	33	-2.029178015174967
4	34	-2.297426870964504
4	35	-1.9384842107573093
4	36	-1.033757286901924
4	37	-1.45745759822519
4	38	-0.9200282520438599
4	39	-1.082152268959451
4	40	-0.4930056916231358
4	41	-0.11713888907236125
4	42	-0.9525862182015139
4	43	-1.1097610553288106
4	44	-0.5543205782490936
4	45	-0.2298574975464085
4	46	-0.6628975439761362
4	47	0.1513618046182792
4	48	-0.18130658136000832
4	49	0.47126360915654786
4	50	-0.10373093710102665
4	51	0.35297508073309203
4	52	-0.1876238563460646
4	53	0.7721602920750854
4	54	1.0879434631920937
4	55	0.14839136556616594
4	56	0.8943684760585526
4	57	1.369203417582515
4	58	1.005160394403532
5	1	0.1284956228188392
5	2	-0.5612002250480401
5	3	-0.8252109538106964
5	4	-0.10167658406392377
5	5	0.1379509379555941
5	6	-0.5845953670276316
5	7	-0.46469848053591534
5	8	-0.73431528523993
5	9	-0.3821815272212845
5	10	-0.5229015044227623
5	11	-0.8775892427271756
5	12	-0.7417004727953227
5	13	-0.27922792752283243
5	14	-0.5252531903548103
5	15	-0.182619230559375
5	16	-0.017649208218955437
5	17	-0.47965171612257507
5	18	0.19679538547305242
5	19	-0.33783624127054424
5	20	-0.8886510092284254
5	21	-0.4398498416690799
5	22	-1.3712386827800458
5	23	-1.0036542609320307
5	24	-0.4581786252306159
5	25	-0.7345714000319745
5	26	-1.17785149649837
5	27	-1.0318899387292646
5	28	-0.7675574492556194
5	29	-1.1607111838073862
5	30	-0.27189523966831075
5	31	-0.8061880036149205
5	32	-0.6033711177933299
5	33	-1.3496317549835446
5	34	-0.3308665495654708
5	35	-1.6851461509676362
5	36	-0.7173210349818007
5	37	-0.15910004180311788
5	38	0.1669385446003595
5	39	-0.09940383459811386
5	40	0.09153879614715876
5	41	0.047223630128868876
5	42	-0.27185834392525265
5	43	-0.10893392546942238
5	44	-0.12208614601466294
5	45	-0.03796337016840923
5	46	0.6486940901961447
5	47	-0.578403054178839
5	48	0.18463930071490608
5	49	0.7302734165534517
5	50	0.38474832557178473
5	51	0.5562127033255528
5	52	-0.47463791755367524
5	53	-0.24131010849209034
5	54	0.36503196608704513
5	55	0.7822091606742809
5	56	-0.48516596799202105
5	57	0.15745817357920444
5	58	-2.7503038673160063E-4
6	1	0.045467133737801646
6	2	0.06245568893045572
6	3	0.4796332984894889
6	4	-0.40718595109640177
6	5	0.1555764783613185
6	6	-0.044633056862458886
6	7	0.1743659247406626
6	8	-0.07851215920068455
6	9	-0.179432386327818
6	10	0.28798420669573943
6	11	0.17994318161602507
6	12	-0.011404496739451986
6	13	0.5296624393987983
6	14	-0.246680969980126
6	15	0.47484668231059857
6	16	0.18975573580055283
6	17	-0.18145861114529538
6	18	-0.48461465775940127
6	19	-0.657423870136526
6	20	-0.47472794083245873
6	21	0.14722476203338886
6	22	0.12305241576778769
6	23	0.052769638857348866
6	24	0.15328351865586853
6	25	0.28826420577669154
6	26	1.5281677918288636
6	27	1.1217515644300278
6	28	1.0517530484719018
6	29	1.1981182142637792
6	30	1.4802386700977905
6	31	1.4621857908646305
6	32	2.352398752509302
6	33	1.7913725682622115
6	34	1.5857724039873489
6	35	2.3365809302872123
6	36	2.556817087177606
6	37	2.34915846612283
6	38	3.0414535651080605
6	39	2.5383303412138822
6	40	3.1986222680460656
6	41	3.1185017589394235
6	42	2.541466121211652
6	43	2.7070509985966495
6	44	3.3280856752911774
6	45	2.7860029306851573
6	46	3.5322990120451614
6	47	2.330697932408018
6	48	2.9949707495564972
6	49	2.6698588283703266
6	50	2.3218949833702567
6	51	2.3292977271588313
6	52	1.9559130191495733
6	53	2.506611407969501
6	54	1.6375073014157238
6	55	2.3013312092276585
6	56	2.4006560029646344
6	57	2.3374825611193906
6	58	2.1441657189758665
7	1	-0.8791312839078458
7	2	-1.3445210552555906
7	3	-0.9908773704148501
7	4	-0.9521391997178341
7	5	-0.9985831955777869
7	6	-0.7405093455222556
7	7	-1.1127354897882284
7	8	-2.203182502044129
7	9	-1.750398581484852
7	10	-0.7484578935200332
7	11	-1.0347535337999432
7	12	-0.7762090999521121
7	13	-0.8903807157198784
7	14	-0.9501094044083231
7	15	-0.7913964248579504
7	16	-0.3892421064435112
7	17	0.25153107957635257
7	18	-0.20530513965822905
7	19	-0.7215571715031004
7	20	-0.0603189262076711
7	21	-0.11026744607018202
7	22	0.15601813142350096
7	23	0.4750904252395145
7	24	0.31163882913297414
7	25	-0.4831335405671098
7	26	0.483813919855713
7	27	0.3218234896278247
7	28	-0.1492778793631473
7	29	-0.1742145596513467
7	30	-0.45706653249151685
7	31	0.04489976832806519
7	32	0.05079242309189169
7	33	-0.4186490092971269
7	34	-1.922139367770022
7	35	-0.7280318723979917
7	36	-0.71482040982551
7	37	-0.6187149455290495
7	38	-0.5272349987854916
7	39	-0.012257047901439222
7	40	-0.6372684056532911
7	41	-0.12544453691289448
7	42	-0.7406005294716387
7	43	0.11474699476063677
7	44	-1.0716985491154354
7	45	-0.4579433651671607
7	46	-0.5459795782209618
7	47	-0.1082855247964929
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117	43	-1.147386473389009
117	44	-1.1728107836958195
117	45	-0.6763457918607898
117	46	-0.6738865549651472
117	47	-1.2609649990128737
117	48	-0.5669969779199668
117	49	-1.0608673034091467
117	50	-1.0307495706413974
117	51	0.10787235736949796
117	52	0.1727440366305224
117	53	-0.37595246473332367
117	54	-0.005702021757741704
117	55	-0.8899507271237539
117	56	1.0612342190112507
117	57	0.4687017453417113
117	58	0.28502183730845587
118	1	-0.08175374803451518
118	2	-1.1077570770269887
118	3	-0.7838016653183644
118	4	-0.46772554311219494
118	5	-0.9080339625248399
118	6	-1.261785941266936
118	7	-1.9301526100257291
118	8	-2.5013022447457587
118	9	-3.055940392469516
118	10	-1.2791357757565507
118	11	-0.998385399213106
118	12	-0.7235124151426078
118	13	-0.2966152530776256
118	14	-0.9986002505241061
118	15	-0.6167304179306825
118	16	-0.12105803915009261
118	17	-0.7059157289342863
118	18	-0.6412279501480853
118	19	-0.13570858169607902
118	20	-0.5105910687113777
118	21	0.2222412130094824
118	22	-0.2729774153355251
118	23	0.24228488342852472
118	24	0.21020389593159194
118	25	-0.04251008683133506
118	26	0.02290347947966609
118	27	0.08753353416023657
118	28	0.010296588721956823
118	29	-0.06016211036881458
118	30	-0.30392452358503164
118	31	0.36677012953538934
118	32	-0.20254750763547236
118	33	0.10163797734315239
118	34	-0.6821591599521106
118	35	-0.20081762202688935
118	36	-0.713088593892806
118	37	-1.4455333522583906
118	38	-1.8441573144707952
118	39	-1.2034990073274656
118	40	-1.7904104145597728
118	41	-1.304068980257771
118	42	-0.6072507026532321
118	43	-0.7734271764417393
118	44	-0.9235045857309743
118	45	-0.8280571702141657
118	46	-0.11005676773110551
118	47	-0.21887434221361346
118	48	-1.2180145542381915
118	49	0.10032603609131385
118	50	-0.47455160579403355
118	51	-0.21775206446213957
118	52	-0.7359053620743801
118	53	-0.9818729087666291
118	54	-0.101909237298905
118	55	0.11028054022129652
118	56	-0.5518555127186282
118	57	-0.870567449847929
118	58	-0.641203879620317
119	1	0.437219711057395
119	2	-0.3396061667396495
119	3	0.13942711292248333
119	4	0.2422985849860737
119	5	0.1452672000107995
119	6	-0.24050934552225556
119	7	-0.598829951547998
119	8	-0.3362441179635738
119	9	-0.6536591904515276
119	10	-0.1965518757784892
119	11	0.08715967646138978
119	12	-0.2794457338528531
119	13	0.014198521873019843
119	14	-0.05080468132033644
119	15	0.021238434887919766
119	16	0.6124361481716889
119	17	0.3649331414320258
119	18	0.2061069917251288
119	19	0.12608128450265355
119	20	-0.07266608516235351
119	21	0.13691548368287698
119	22	0.3498565394791271
119	23	0.47545005122848494
119	24	0.8440051681404057
119	25	0.6181611129931923
119	26	1.4456935650247402
119	27	1.101133007728997
119	28	1.154845831977056
119	29	0.9300769771503781
119	30	0.4080737216441861
119	31	1.101361048596587
119	32	0.9400276184886778
119	33	1.0697230837261316
119	34	1.2043291050182745
119	35	1.4396737137923665
119	36	0.7836212108889455
119	37	0.32853990942179934
119	38	0.9280515032523873
119	39	0.06410353709016903
119	40	0.7243954639223489
119	41	1.1185017589394235
119	42	1.2837341624487628
119	43	0.5936489367409763
119	44	1.6579825825076782
119	45	1.1365183946026818
119	46	0.944670146065782
119	47	0.629667004572962
119	48	0.531053223783303
119	49	0.10284851909197457
119	50	0.5590083854321115
119	51	0.17465855190109636
119	52	0.059005802654727546
119	53	0.3416629543612508
119	54	1.2251361673951067
119	55	-0.7192873474733723
119	56	-0.7024367805405198
119	57	-1.219218469808446
119	58	-0.7218136624674294
120	1	-0.8821362486321718
120	2	-3.1734993672493204
120	3	-1.6967827793513521
120	4	-1.7462337907813321
120	5	-1.6565398195652463
120	6	-1.4427565365334942
120	7	-2.644352944713752
120	8	-3.1456962203613514
120	9	-3.917762051565859
120	10	-1.6089230097991063
120	11	-1.7380459299326816
120	12	-1.593820806249468
120	13	-1.3406028218081971
120	14	-1.6490903294148538
120	15	-1.97783488840642
120	16	-1.0976298775435893
120	17	-1.4563340889629757
120	18	-0.6111060280825811
120	19	-1.011993544641328
120	20	-0.3734885107386319
120	21	-1.068459241209979
120	22	-0.3931065092872359
120	23	-0.9527904213766405
120	24	-1.18078343373611
120	25	-0.38867312771108686
120	26	-0.6664343163606432
120	27	-0.7386677567792788
120	28	-1.244783497340677
120	29	-0.4956151345141606
120	30	-1.125344557053836
120	31	-0.9638538245645378
120	32	-1.0832551337411367
120	33	-1.5257825342513982
120	34	-0.6332708023140512
120	35	-0.5852307226723603
120	36	1.208618315024239
120	37	0.893696865288824
120	38	1.1801075671930796
120	39	1.3516049850225222
120	40	1.2782006301449869
120	41	0.43021263580725133
120	42	1.1338442053075575
120	43	0.7095993595372221
120	44	0.6119962509890868
120	45	0.513444144631638
120	46	0.15757411919215514
120	47	0.689205982911897
120	48	-0.4962837390337924
120	49	1.1219612261463006
120	50	0.7004424176341253
120	51	0.3765582391477089
120	52	0.7302672413203126
120	53	0.9547754297464017
120	54	0.7759296343243314
120	55	0.5310311976442001
120	56	2.4033201984934216
120	57	2.1859709197432764
120	58	2.4378064000832467
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	174.0
3	18.0
4	69.0
5	36.0
6	54.0
7	146.0
8	134.0
9	235.0
10	297.0
11	349.0
12	468.0
13	532.0
14	640.0
15	768.0
16	814.0
17	928.0
18	971.0
19	984.0
20	1057.0
21	1041.0
22	1273.0
23	1322.0
24	1467.0
25	1171.0
26	1229.0
27	1307.0
28	1732.0
29	2082.0
30	2577.0
31	3029.0
32	3927.0
33	4889.0
34	6195.0
35	8020.0
36	10115.0
37	14436.0
38	18668.0
39	16063.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	100.0	0.0	0.0	0.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	45.21057015496426	31.58872687664186	5.3079605678666555	17.892742400527226
11	28.074800230661506	25.555830152584413	18.146286990508095	28.223082626245983
12	36.85549789017949	12.977455583930581	30.736560763745867	19.430485762144052
13	18.059331264702383	21.251979386916368	31.093536901264063	29.59515244711719
14	21.653806372481714	26.01898380792853	36.902179385085724	15.42503043450403
15	32.635856880028555	14.773320152675948	36.54611857099706	16.04470439629843
16	21.913758226469323	20.74580553038416	33.40106726711884	23.93936897602768
17	30.178213471730235	13.959597623820377	22.268903717128445	33.593285187320944
18	31.996301111497683	15.90247386057754	28.790902931643807	23.31032209628097
19	28.099771167048054	17.91395881006865	33.59816933638444	20.388100686498856
20	31.135641779022617	25.080777292656357	24.817164144950617	18.966416783370406
21	32.592219679633864	17.68878718535469	30.043020594965675	19.675972540045766
22	32.15714272638237	17.779242295265032	22.68629120099587	27.377323777356725
23	32.17819516526165	28.418046516736688	20.53253517130278	18.87122314669889
24	28.676174435209255	30.448354143019294	20.852403793343342	20.023067628428105
25	26.034105575337072	20.577762725516937	22.329723841865828	31.05840785728016
26	31.57788742322022	17.967612892595273	25.782444320355914	24.672055363828598
27	24.072662484961473	19.02614637192686	24.65859101637477	32.24260012673689
28	22.330043759269092	28.735558525733744	25.92233187469103	23.012065840306132
29	31.024727865310492	18.268042369700353	28.4860247736407	22.22120499134845
30	23.092634317011964	23.803374182557565	30.803612317048596	22.300379183381878
31	34.35225618631732	16.17002187983485	30.187763771021576	19.289958162826252
32	32.9441926475301	13.696836515130705	36.576477590074624	16.782493247264572
33	32.533330891509785	14.132664273679584	34.2365028203062	19.097502014504432
34	23.883166231744724	17.288833951380305	40.839628256191915	17.988371560683056
35	22.352251707781623	29.837188432869986	34.33877259491237	13.471787264436019
36	28.208744710860366	25.89434542890114	30.415071529316943	15.481838330921546
37	23.860336884142264	22.396658636892386	37.47858981287268	16.264414666092676
38	25.373052296023523	21.975505418304067	35.95134060659357	16.700101679078845
39	23.849268882618098	22.578517242643017	34.8572580349617	18.714955839777183
40	24.980993817265855	18.77994046256011	38.30089306159835	17.93817265857568
41	21.888227210261107	22.685295464956482	37.687585890975726	17.73889143380669
42	27.120817911979184	19.29348821869618	36.386456081204884	17.199237788119756
43	24.125201524256195	16.209878352630806	38.96013483804778	20.70478528506522
44	20.915254237288135	19.862574438845627	38.37746220797068	20.844709115895558
45	26.31868232535131	18.332386727982264	35.4665542954508	19.882376651215626
46	19.71286430227125	21.6991763401651	39.294712635253376	19.29324672231028
47	25.446064460552964	19.866936703292676	39.24175914810164	15.44523968805271
48	25.678858473015204	19.13817278700844	37.25359018301456	17.92937855696179
49	22.534139858858033	20.77261479241133	36.38163321418752	20.31161213454312
50	17.27788522351259	19.590463707275045	40.855553213136695	22.276097856075676
51	17.707875054993398	21.763638363396392	42.540145182578094	17.988341399032116
52	17.065303055295907	20.249812136874322	42.68616777551731	19.998717032312456
53	16.089183765109098	19.953630307084666	43.24019684209562	20.716989085710622
54	17.549568708692743	21.878981767515192	41.29304892245923	19.27840060133283
55	18.757679681625618	21.242687109138593	35.277018724668515	24.72261448456728
56	18.77831789415757	20.72090250389801	38.17573145005962	22.3250481518848
57	20.212765957446805	22.117571533382243	36.90663976522377	20.763022743947175
58	19.944245247549265	22.968573760419627	37.482232757751106	19.604948234280002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	46.0
10	92.0
11	122.0
12	152.0
13	161.0
14	170.0
15	246.5
16	633.0
17	943.0
18	1081.5
19	1220.0
20	1206.0
21	1192.0
22	1317.5
23	1798.0
24	2153.0
25	2482.0
26	2811.0
27	3047.5
28	3422.5
29	3561.0
30	3326.5
31	3092.0
32	3161.5
33	3231.0
34	3519.0
35	2744.5
36	1682.0
37	1681.5
38	1681.0
39	1555.0
40	1429.0
41	1446.5
42	1563.0
43	1662.0
44	2028.5
45	2395.0
46	2952.5
47	2866.5
48	2223.0
49	2502.0
50	2781.0
51	2783.0
52	2785.0
53	3979.5
54	5213.0
55	5252.0
56	5126.5
57	5001.0
58	5619.0
59	7129.5
60	8022.0
61	8456.5
62	8891.0
63	8537.5
64	8184.0
65	7134.0
66	4633.5
67	3183.0
68	2536.5
69	1890.0
70	1438.0
71	986.0
72	797.0
73	479.0
74	350.0
75	243.5
76	137.0
77	105.0
78	63.5
79	54.0
80	35.5
81	17.0
82	13.0
83	9.0
84	7.0
85	5.0
86	5.0
87	4.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.026544379456480947
19	9.153234295338258E-4
20	0.0
21	9.153234295338258E-4
22	0.0
23	0.0
24	0.006407264006736781
25	0.0018306468590676517
26	0.009153234295338257
27	0.33317772835031256
28	0.015560498302075038
29	0.020137115449744166
30	0.06315731663783397
31	0.016475821731608865
32	0.03295164346321773
33	0.039358907469954504
34	0.03295164346321773
35	0.04118955432902216
36	0.059496022919698674
37	0.0677339337855031
38	0.07688716808084137
39	0.09427831324198406
40	0.06956458064457076
41	0.0924476663829164
42	0.08695572580571344
43	0.07597184465130753
44	0.0924476663829164
45	0.08146378522851049
46	0.09519363667151788
47	0.11899204583939735
48	0.12265333955753266
49	0.12906060356426943
50	0.13912916128914152
51	0.13455254414147239
52	0.11807672240986351
53	0.1171613989803297
54	0.1464517487254121
55	0.18031871561816368
56	0.20228647792697552
57	0.19313324363163722
58	0.1848953327658328
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
58	109251.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.3877859241563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.66363224999336	24.98741430284391
2	10.790811573371663	7.421442366660259
3	4.828448987196891	4.98119010352308
4	2.709680853895499	3.727197005061739
5	1.892517767308153	3.2539747919927504
6	1.251031435492028	2.581212071285389
7	0.9342809231014931	2.2489496663646102
8	0.7027070190848839	1.93316308317544
9	0.5855891825707364	1.8123403904769753
>10	3.199446352045569	19.507372930224896
>50	0.2821475152386276	6.874994279228565
>100	0.1251031435492028	9.191677879378679
>500	0.021294152093481327	5.2054443437588676
>1k	0.013308845058425832	6.273626786024841
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	2009	1.8388847699334558	No Hit
AGGACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	1331	1.2182954847095222	No Hit
AGGACTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT	1315	1.2036503098369808	No Hit
AGGACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	1141	1.0443840330980951	No Hit
AGGACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	1058	0.9684121884467876	No Hit
AGGACTGGGATCCATATTTTCCCTTTTTGTTGAGTATAGGCTTTAATTCTTTGAATTT	893	0.8173838225737063	No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	880	0.8054846179897667	No Hit
AGGACTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	715	0.6544562521166853	No Hit
AGGACTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT	709	0.6489643115394824	No Hit
AGGACTGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC	679	0.6215046086534677	No Hit
AGGACTGGGAGTGCAGTGCTAGGACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAG	665	0.6086900806399942	No Hit
AGGACTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	589	0.5391254999954234	No Hit
AGGACTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	557	0.509835150250341	No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA	500	0.45766171476691286	No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	486	0.4448471867534393	No Hit
AGGACTGGGAACTCTAGCCTGATTGGCTTAGGGTACACTCAGACCCTAAAACCAGGTA	483	0.44210121646483785	No Hit
AGGACTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC	431	0.3945043981290789	No Hit
AGGACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGG	399	0.3652140483839965	No Hit
AGGACTGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	371	0.33958499235704936	No Hit
AGGACTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	362	0.3313470814912449	No Hit
AGGACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	347	0.31761723004823755	No Hit
AGGACTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	322	0.2947341443098919	No Hit
AGGACTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	316	0.28924220373268894	No Hit
AGGACTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	275	0.25171394312180206	No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	273	0.24988329626273445	No Hit
AGGACTGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	266	0.24347603225599768	No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCAG	266	0.24347603225599768	No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGGACACTGACTGCTCTTTCAAA	247	0.22608488709485497	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAGCAGTGG	237	0.21693165279951673	No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA	235	0.21510100594044906	No Hit
AGGACTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	201	0.183980009336299	No Hit
AGGACTGGGTGTCTGGGAATAGATTTTTCTGTGTAGTTCTTTTAGGAATCATAAAGGT	193	0.17665742190002837	No Hit
AGGACTGGGGCTAGGACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGA	191	0.17482677504096072	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTC	184	0.16841951103422392	Illumina Single End Adapter 2 (96% over 25bp)
AGGACTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	183	0.16750418760469013	No Hit
AGGACTGGGAGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	172	0.15743562987981802	No Hit
AGGACTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	169	0.15468965959121656	No Hit
AGGACTGGGGTGCTAGGACTGGGACTAACTAACCATTTGCTTACAAATATCCTGCTGG	162	0.14828239558447978	No Hit
AGGACTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT	160	0.1464517487254121	No Hit
AGGACTGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG	158	0.14462110186634447	No Hit
AGGACTGGGAGTATAAATTAGTCTTTAACTACATAAACATTTGAGAAATCACCTAATT	148	0.1354678675710062	No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA	147	0.13455254414147239	No Hit
AGGACTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA	140	0.1281452801347356	No Hit
AGGACTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	138	0.12631463327566794	No Hit
AGGACTGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGAT	130	0.11899204583939735	No Hit
AGGACTGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGATCT	129	0.11807672240986351	No Hit
AGGACTGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT	129	0.11807672240986351	No Hit
AGGACTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC	129	0.11807672240986351	No Hit
AGGACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC	123	0.11258478183266056	No Hit
AGGACTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT	122	0.11166945840312675	No Hit
AGGACTGGGGGGCTGGAGAGATGGCTTGGCGGTTAAGAGCACTGGCTGCTCTTCCGAA	121	0.1107541349735929	No Hit
AGGACTGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCAA	116	0.10617751782592379	No Hit
AGGACTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG	116	0.10617751782592379	No Hit
AGGACTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	116	0.10617751782592379	No Hit
AGGACTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	115	0.10526219439638995	No Hit
AGGACTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC	114	0.10434687096685615	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.010983881154405909	0.0	0.0	0.0	0.0
11	0.03661293718135303	0.0	0.0	0.0	0.0
12	0.03935890746995451	0.0	0.0	0.0	0.0
13	0.03935890746995451	0.0	0.0	0.0	0.0
14	0.05583472920156337	0.0	0.0	0.0	0.0
15	0.05583472920156337	0.0	0.0	0.0	0.0
16	0.05858069949016485	0.0	0.0	0.0	0.0
17	0.05858069949016485	0.0	0.0	0.0	0.0
18	0.0604113463492325	0.0	0.0	0.0	0.0
19	0.06132666977876633	0.0	0.0	0.0	0.0
20	0.07688716808084137	0.0	0.0	0.0	0.0
21	0.07963313836944284	0.0	0.0	0.0	0.0
22	0.08420975551711198	0.0	0.0	0.0	0.0
23	0.09885493038965318	0.0	0.0	0.0	0.0
24	0.1135001052621944	0.0	0.0	0.0	0.0
25	0.120822692698465	0.0	0.0	0.0	0.0
26	0.12356866298706648	0.0	0.0	0.0	0.0
27	0.12906060356426943	0.0	0.0	0.0	0.0
28	0.2196776230881182	0.0	0.0	0.0	0.0
29	0.2196776230881182	0.0	0.0	0.0	0.0
30	0.25262926655133594	0.0	0.0	0.0	0.0
31	0.2562905602694712	0.0	0.0	0.0	0.0
32	0.25720588369900504	9.153234295338258E-4	0.0	0.0	0.0
33	0.2599518539876065	9.153234295338258E-4	0.0	0.0	0.0
34	0.262697824276208	9.153234295338258E-4	0.0	0.0	0.0
35	0.34965355008192145	9.153234295338258E-4	0.0	0.0	0.0
36	0.36429872495446264	9.153234295338258E-4	0.0	0.0	0.0
37	0.36704469524306416	9.153234295338258E-4	0.0	0.0	0.0
38	0.37070598896119944	9.153234295338258E-4	0.0	0.0	0.0
39	0.37528260610886854	9.153234295338258E-4	0.0	0.0	0.0
40	0.38168987011560535	9.153234295338258E-4	0.0	0.0	0.0
41	0.3862664872632745	9.153234295338258E-4	0.0	0.0	0.0
42	0.39267375127001125	9.153234295338258E-4	0.0	0.0	0.0
43	0.3999963387062819	9.153234295338258E-4	0.0	0.0	0.0
44	0.4054882792834848	9.153234295338258E-4	0.0	0.0	0.0
45	0.4064036027130186	9.153234295338258E-4	0.0	0.0	0.0
46	0.4118955432902216	9.153234295338258E-4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTAGG	45	8.913048E-11	52.155407	26
TAGCGCG	15	0.007118179	52.155403	23
AACGCGG	20	3.3835793E-4	52.155403	23
AACGCGA	20	3.3835793E-4	52.155403	23
TAACTAC	25	1.6164244E-5	52.155403	26
ACTTACC	25	1.6164244E-5	52.155403	25
AGTAAGC	15	0.007118179	52.155403	26
AGACGAA	15	0.007118179	52.155403	26
CTTACCC	25	1.6164244E-5	52.155403	26
CGGCTTA	15	0.007118179	52.155403	25
TTAACTA	25	1.6164244E-5	52.155403	25
ACGCGGA	15	0.007118179	52.155403	24
ACGCGAT	15	0.007118179	52.155403	24
CTTCCTA	15	0.007118179	52.155403	27
TTTAACT	25	1.6164244E-5	52.155403	24
TTGCCGG	15	0.007118179	52.155403	26
ACCAGCG	25	1.6164244E-5	52.155403	27
GCTTACA	15	0.007118179	52.155403	27
CGCGATC	15	0.007118179	52.155403	25
CTTTTTG	105	0.0	52.155403	23
>>END_MODULE