FastQCFastQC Report
Tue 31 May 2016
SRR212559_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212559_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105696
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT22202.1003633060853772No Hit
AGGACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC10671.009498940357251No Hit
AGGACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC10450.9886845292158645No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC10410.9849000908265213No Hit
AGGACTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT9110.8619058431728731No Hit
AGGACTGGGATCCATATTTTCCCTTTTTGTTGAGTATAGGCTTTAATTCTTTGAAT8940.8458219800181653No Hit
AGGACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA8900.8420375416288224No Hit
AGGACTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA6040.5714501967907962No Hit
AGGACTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA5610.5307674841053588No Hit
AGGACTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5450.5156297305479867No Hit
AGGACTGGGAGTGCAGTGCTAGGACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAG5180.49008477141992124No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4880.46170148349984863No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC4390.41534211323039666No Hit
AGGACTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT4270.4039887980623676No Hit
AGGACTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC4150.3926354828943384No Hit
AGGACTGGGAACTCTAGCCTGATTGGCTTAGGGTACACTCAGACCCTAAAACCAGG3680.348168331819558No Hit
AGGACTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT3490.3301922494701786No Hit
AGGACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.30180896155010595No Hit
AGGACTGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC3080.29140175597941265No Hit
AGGACTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2870.2715334544353618No Hit
AGGACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA2870.2715334544353618No Hit
AGGACTGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2830.2677490160460188No Hit
AGGACTGGGAGATATAATTTCCTCAAAGCATGACAGATTTTTTTAAAGATTATATC2640.24977293369663942No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAGCAGT2630.24882682409930368No Hit
AGGACTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2620.24788071450196789No Hit
AGGACTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2490.23558128973660308No Hit
AGGACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2450.23179685134726005No Hit
AGGACTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC2440.23085074174992432No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC2300.21760520738722372No Hit
AGGACTGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2180.20625189221919465No Hit
AGGACTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC2140.20246745382985165No Hit
AGGACTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.19962912503784438No Hit
AGGACTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.19773690584317288No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1830.17313805631244322No Hit
AGGACTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.17029972752043596No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGGACACTGACTGCTCTTTCA1770.1674613987284287No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1660.1570541931577354No Hit
AGGACTGGGTGTCTGGGAATAGATTTTTCTGTGTAGTTCTTTTAGGAATCATAAAG1630.15421586436572812No Hit
AGGACTGGGAGTATAAATTAGTCTTTAACTACATAAACATTTGAGAAATCACCTAA1540.14570087798970632No Hit
AGGACTGGGGTGCTAGGACTGGGACTAACTAACCATTTGCTTACAAATATCCTGCT1530.14475476839237056No Hit
AGGACTGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT1510.14286254919769906No Hit
AGGACTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1480.1400242204056918No Hit
AGGACTGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA1460.1381320012110203No Hit
AGGACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC1410.1334014532243415No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAGCAGG1360.12867090523766272No Hit
AGGACTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT1320.12488646684831971No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG1310.12394035725098394Illumina Single End Adapter 2 (95% over 23bp)
AGGACTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG1290.12204813805631244No Hit
AGGACTGGGGCTAGGACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCT1290.12204813805631244No Hit
AGGACTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA1250.11826369966696942No Hit
AGGACTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1250.11826369966696942No Hit
AGGACTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCC1240.11731759006963366No Hit
AGGACTGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCC1230.11637148047229792No Hit
AGGACTGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG1220.11542537087496216No Hit
AGGACTGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC1200.11353315168029066No Hit
AGGACTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG1140.10785649409627612No Hit
AGGACTGGGAGTCTTATTTACCTTTAATTGGTGTTTTTTAGGTCATCAGCATAAAG1120.1059642749016046No Hit
AGGACTGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC1110.10501816530426883No Hit
AGGACTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC1100.10407205570693309No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC1080.10217983651226158No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGG204.0040497E-450.40412550
ACGCACC251.9778658E-550.4041250
TTCTTCG150.00814630250.4041250
AAGTCTC150.00814630250.4041250
CTTCCCC251.9778658E-550.4041250
CATAAAG451.2369128E-1050.4041250
AATCATA251.9834877E-550.37997447
TATCCTG204.0135387E-450.37997448
ATCATAA251.9834877E-550.37997448
AATATCC204.0135387E-450.37997446
ATATCCT204.0135387E-450.37997447
CGCGCGA204.0135387E-450.37997449
GAAATCC150.00816175950.3799747
GCGGCTT150.00816175950.3799749
CTCCACT150.00816175950.3799746
AACTTTC150.00816175950.3799746
GGCTTGA150.00816175950.3799748
CACTTCC150.00816175950.3799749
AGCATAA150.00816175950.3799748
CGGCTTG150.00816175950.3799747
Produced by FastQC (version 0.11.5)