FastQCFastQC Report
Tue 31 May 2016
SRR212558_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212558_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98191
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT19581.9940727765273805No Hit
AGGACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC11601.1813710014156085No Hit
AGGACTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT10641.0836023668156958No Hit
AGGACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC10341.0530496685032233No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC9981.0163864305282562No Hit
AGGACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA8100.824922854436761No Hit
AGGACTGGGATCCATATTTTCCCTTTTTGTTGAGTATAGGCTTTAATTCTTTGAAT7350.7485411086555794No Hit
AGGACTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC6740.6864172887535517No Hit
AGGACTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA6280.6395698180077604No Hit
AGGACTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA5560.5662433420578261No Hit
AGGACTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5460.5560591092870019No Hit
AGGACTGGGAGTGCAGTGCTAGGACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAG5170.5265248342516117No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4410.4491246651933477No Hit
AGGACTGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC4260.4338483160371114No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC4220.4297746229287817No Hit
AGGACTGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT4190.42671935309753445No Hit
AGGACTGGGAACTCTAGCCTGATTGGCTTAGGGTACACTCAGACCCTAAAACCAGG3620.3686692263038364No Hit
AGGACTGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT3050.3106190995101384No Hit
AGGACTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT3030.3085822529559736No Hit
AGGACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.30349013657056145No Hit
AGGACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA2970.30247171329347905No Hit
AGGACTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2930.2983980201851494No Hit
AGGACTGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2860.2912690572455724No Hit
AGGACTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2520.2566426658247701No Hit
AGGACTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC2520.2566426658247701No Hit
AGGACTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2500.2546058192706052No Hit
AGGACTGGGGTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2400.24442158649978105No Hit
AGGACTGGGAGATATAATTTCCTCAAAGCATGACAGATTTTTTTAAAGATTATATC2290.23321893045187442No Hit
AGGACTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.2220162744039678No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC2130.2169241580185557No Hit
AGGACTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2070.21081361835606116No Hit
AGGACTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC2030.2067399252477315No Hit
AGGACTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.19859253903107207No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAGCAGT1950.19859253903107207No Hit
AGGACTGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACAC1860.1894267295373303No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGGACACTGACTGCTCTTTCA1800.18331618987483578No Hit
AGGACTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1800.18331618987483578No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1620.1649845708873522No Hit
AGGACTGGGTGTCTGGGAATAGATTTTTCTGTGTAGTTCTTTTAGGAATCATAAAG1600.16294772433318735No Hit
AGGACTGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT1500.15276349156236316No Hit
AGGACTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.15072664500819832No Hit
AGGACTGGGGTGCTAGGACTGGGACTAACTAACCATTTGCTTACAAATATCCTGCT1390.14156083551445653No Hit
AGGACTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA1370.13952398896029167No Hit
AGGACTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1370.13952398896029167No Hit
AGGACTGGGAGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATC1350.13748714240612683No Hit
AGGACTGGGGCTAGGACTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCT1300.13239502602071473No Hit
AGGACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC1250.1273029096353026No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG1230.12526606308113777Illumina Single End Adapter 2 (95% over 23bp)
AGGACTGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG1190.12119236997280809No Hit
AGGACTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC1190.12119236997280809No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1170.11915552341864326No Hit
AGGACTGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA1130.11508183031031358No Hit
AGGACTGGGGCTAGGACTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCC1130.11508183031031358No Hit
AGGACTGGGAGTATAAATTAGTCTTTAACTACATAAACATTTGAGAAATCACCTAA1120.11406340703323116No Hit
AGGACTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCC1110.11304498375614873No Hit
AGGACTGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGC1100.11202656047906631No Hit
AGGACTGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC1100.11202656047906631No Hit
AGGACTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG1090.1110081372019839No Hit
AGGACTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG1060.10795286737073663No Hit
AGGACTGGGGCTTTCCGATCCGCCATCGTGGGTGGAGCCGCCGCCACGATGCAGAT1010.10286075098532453No Hit
AGGACTGGGCCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC990.10082390443115968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAAGC301.001199E-650.2619450
GAAACAC900.050.2619450
TTATATC355.0120434E-850.2619450
CTGATAC150.00823612650.2619449
GATGAGA150.00823612650.2619450
TGATACA150.00823612650.2619450
TTTTACG150.00823612650.2619449
CTAGACC150.00823612650.2619449
TTTACGG150.00823612650.2619450
TCTTCCA150.00823612650.2619450
CATAAAG252.0101936E-550.2619450
CATCGAA600.050.2619450
TCTAGAC150.00826964250.21036548
GTTTTAC150.00826964250.21036548
GATTATA402.5429472E-950.21036548
TACCAGC204.079537E-450.21036547
TAAAACC355.0527888E-850.21035847
AATTCTT650.050.1846245
TAATTCT650.050.1846244
GAATCAT204.089885E-450.18461646
Produced by FastQC (version 0.11.5)