FastQCFastQC Report
Tue 31 May 2016
SRR212557_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212557_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73802
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA15442.0920842253597463No Hit
ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT14761.9999458009268043No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG9981.3522668762364163No Hit
ATTTGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG7851.0636568114685239No Hit
ATTTGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC7851.0636568114685239No Hit
ATTTGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6070.8224709357469988No Hit
ATTTGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5900.7994363296387632No Hit
ATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5640.7642069320614617No Hit
ATTTGCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT5610.7601420015717731No Hit
ATTTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT5200.7045879515460285No Hit
ATTTGCGGGGTGCTATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC5160.6991680442264437No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4600.623289341752256No Hit
ATTTGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT4260.5772201295357849No Hit
ATTTGCGGGAGTTTCCTTGATGTGACTTACCTCTTTTTGTTTATTTCTTTTGAGACAG4050.5487656161079646No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3890.5270859868296252No Hit
ATTTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3490.47288691363377683No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3360.45527221484512614No Hit
ATTTGCGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA2940.3983631879894854No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA2870.38887835018021194No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA2820.38210346603073087No Hit
ATTTGCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2750.3726186282214574No Hit
ATTTGCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT2740.3712636513915612No Hit
ATTTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA2640.3577138830925991No Hit
ATTTGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2580.34958402211322187No Hit
ATTTGCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT2440.33061434649467497No Hit
ATTTGCGGGGGGGGATGAAGTTAGGAAAGCCACTCGGGTCGTCTTTGGCCGTCCACTT2160.2926749952575811No Hit
ATTTGCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC2120.28725508793799626No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTG1820.24660578304111No Hit
ATTTGCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1820.24660578304111No Hit
ATTTGCGGGAAGATGGAAGTTACCACACCTGTTACAGTTGCCTGAGAATTACAACACT1790.24254085255142138No Hit
ATTTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA1780.24118587572152517No Hit
ATTTGCGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA1750.23712094523183655No Hit
ATTTGCGGGACTGGAGTCCTGGTGTATCTGGCAAAGTATCATGGCTTCGGGAACGTCC1700.23034606108235547No Hit
ATTTGCGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1620.2195062464431858No Hit
ATTTGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAGGAGAACTGACTGCTCTTCTGAA1610.21815126961328962No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA1550.2100214086339124No Hit
ATTTGCGGGGGAGAATGGTTGTAGTTGGAGTAGTACAAAGTGACCAAGCCAAAATCAG1550.2100214086339124No Hit
ATTTGCGGGGAGTGCAGTGCTATTTGCGGGGGGGAATTCCTTCGGCAACATTAACTGG1520.20595647814422371No Hit
ATTTGCGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1510.2046015013143275No Hit
ATTTGCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1500.2032465244844313No Hit
ATTTGCGGGAGTTCATAATGTTGTTCCACATATAGGGTTGCAGATCCCTTTAGATCCT1500.2032465244844313No Hit
ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1340.18156689520609198No Hit
ATTTGCGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCCGA1330.18021191837619577No Hit
ATTTGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1300.17614698788650712No Hit
ATTTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.17614698788650712No Hit
ATTTGCGGGGGAATTCCTTCGGCGACATTAACTGGTTGATGCTGAAAAACATCGAACT1270.1720820573968185No Hit
ATTTGCGGGGACGCTACTCAGAATACCCAGATGCTTACACCACATGAAACACTGTCTC1270.1720820573968185No Hit
ATTTGCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT1270.1720820573968185No Hit
ATTTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1260.17072708056692232No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCTA1220.16530717324733749No Hit
ATTTGCGGGGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGGGAT1180.15988726592775263No Hit
ATTTGCGGGGTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTAC1160.15717731226796022No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA1140.15446735860816782No Hit
ATTTGCGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA1110.15040242811847918No Hit
ATTTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1090.14769247445868675No Hit
ATTTGCGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC1070.14498252079889434No Hit
ATTTGCGGGATCATAAGGGAGGAAAATGTCCCAAAAAGCAGAGAGAAATATCTCTCTG1030.13956261347930948No Hit
ATTTGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC1000.13549768298962087No Hit
ATTTGCGGGGTTCACTGATTCCCATTATTTTCAGGCTTCACCCTAGATGACACATGAG1000.13549768298962087No Hit
ATTTGCGGGGGGATTGGTGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTTTTCCAA990.13414270615972468No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAA980.13278772932982846No Hit
ATTTGCGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA980.13278772932982846No Hit
ATTTGCGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA970.13143275249993225No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC970.13143275249993225No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG970.13143275249993225No Hit
ATTTGCGGGGGGCTGGAGAAATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAGA960.13007777567003606No Hit
ATTTGCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG950.12872279884013985No Hit
ATTTGCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA950.12872279884013985No Hit
ATTTGCGGGCTAGATTCTAAGGATCATGTCTGCGAGTCAGGATTCTCGATCCAGAGAC930.12601284518034742No Hit
ATTTGCGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA930.12601284518034742No Hit
ATTTGCGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCT930.12601284518034742No Hit
ATTTGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC920.1246578683504512No Hit
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC910.123302891520555No Hit
ATTTGCGGGCTTTTCCTCTGGCGCGGTGAGTGAGTTCGGTCACGGTTTTTTACGATGG900.12194791469065878No Hit
ATTTGCGGGGTGTAGCCTGTTGTAGCTGGGGATGTTCTTCACGGGTAGAGTGTTTGCC900.12194791469065878No Hit
ATTTGCGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCTT880.11923796103086638No Hit
ATTTGCGGGGCACTTCTGGGGGTCTTCCATAGCCTGCTCACAGAAAATGCAACCCCAG860.11652800737107397No Hit
ATTTGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG860.11652800737107397No Hit
ATTTGCGGGGTAGATCATGCTCTTTACACAGAGTACCGCTTATTTAATAAGACGGGAT850.11517303054117774No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAATGGTTAAGAGCACTGACTGCTCTTCCGG850.11517303054117774No Hit
ATTTGCGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG830.11246307688138532No Hit
ATTTGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.11110810005148912No Hit
ATTTGCGGGCAGGAATACCACGACGCTACTCAGACTACCCAGATTCTTACACCACATG820.11110810005148912No Hit
ATTTGCGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA810.1097531232215929No Hit
ATTTGCGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGT760.10297823907211186No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCCGACTGCTCTTCCAA760.10297823907211186No Hit
ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT760.10297823907211186No Hit
ATTTGCGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT760.10297823907211186No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA750.10162326224221566No Hit
ATTTGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG740.10026828541231944No Hit
ATTTGCGGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGTCA740.10026828541231944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTGA307.853687E-752.03731547
TTTGAGA307.853687E-752.03731549
TTTTGAG307.853687E-752.03731548
TTGTCAG150.00717312352.03731540
TTCACGG150.00717312352.03731538
ATCTTCG150.00717312352.03731549
ACATGTC150.00717312352.03731537
GGGCAAC150.00717312352.03731542
ACTTGTC150.00717312352.03731538
GAAATAT150.00717312352.03731545
TTATCTC150.00717312352.03731547
TTATTTC307.853687E-752.03731541
TCGAACT458.913048E-1152.03731552
TGTTTAT307.853687E-752.03731538
AATTACA307.853687E-752.03731547
CAATTAT150.00717312352.03731544
TTTCAAA150.00717312352.03731552
TACATTT150.00717312352.03731539
GTTTATT307.853687E-752.03731539
ATTACAA307.853687E-752.03731548
Produced by FastQC (version 0.11.5)