##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212557_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 73802 Sequences flagged as poor quality 0 Sequence length 58 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.68808433375789 39.0 38.0 40.0 33.0 40.0 2 37.304151649006805 39.0 38.0 40.0 33.0 40.0 3 36.64552451153085 39.0 36.0 40.0 31.0 40.0 4 36.536191431126525 39.0 36.0 39.0 31.0 40.0 5 36.80069645809056 39.0 36.0 39.0 31.0 40.0 6 37.26743177691662 39.0 37.0 39.0 33.0 40.0 7 37.47280561502399 39.0 38.0 40.0 33.0 40.0 8 37.29308148830655 39.0 38.0 40.0 33.0 40.0 9 37.302186932603455 39.0 38.0 40.0 33.0 40.0 10 36.428687569442566 39.0 35.0 39.0 31.0 40.0 11 36.63349231728137 39.0 35.0 39.0 31.0 40.0 12 36.799029836589796 39.0 35.0 39.0 31.0 40.0 13 36.6945475732365 39.0 35.0 39.0 31.0 40.0 14 36.73206688165632 39.0 36.0 39.0 31.0 40.0 15 36.80321671499417 38.0 36.0 39.0 32.0 40.0 16 36.672393702067694 38.0 36.0 39.0 32.0 40.0 17 36.24750006774884 38.0 35.0 39.0 31.0 40.0 18 36.15170320587518 38.0 35.0 39.0 30.0 40.0 19 36.079848784585785 38.0 35.0 39.0 30.0 40.0 20 35.975014227256715 38.0 35.0 39.0 30.0 40.0 21 35.86364868160754 38.0 35.0 39.0 30.0 40.0 22 35.73336765941303 38.0 35.0 39.0 30.0 40.0 23 35.54339990786158 38.0 35.0 39.0 29.0 40.0 24 35.43764396628818 38.0 35.0 39.0 29.0 40.0 25 35.504769518441236 38.0 35.0 39.0 29.0 40.0 26 35.327985691444674 38.0 35.0 39.0 29.0 40.0 27 35.19464242161459 38.0 35.0 39.0 28.0 40.0 28 34.86863499634156 38.0 33.0 39.0 28.0 40.0 29 34.84650822470936 38.0 33.0 39.0 28.0 40.0 30 34.50887509823582 38.0 33.0 39.0 27.0 40.0 31 34.09320885612856 37.0 33.0 38.0 26.0 40.0 32 34.006883282295874 37.0 33.0 38.0 25.0 40.0 33 33.728909785642664 37.0 33.0 38.0 25.0 39.0 34 33.062681228151 36.0 31.0 38.0 23.0 39.0 35 32.82085851331942 36.0 31.0 38.0 22.0 39.0 36 33.83454377930138 38.0 33.0 39.0 25.0 40.0 37 33.63461694805019 37.0 33.0 39.0 24.0 40.0 38 33.33083114278746 37.0 33.0 38.0 23.0 40.0 39 33.35310696187095 37.0 33.0 38.0 23.0 40.0 40 33.22301563643261 37.0 33.0 38.0 23.0 40.0 41 33.35301211349286 38.0 33.0 39.0 22.0 40.0 42 33.253448416032086 38.0 33.0 39.0 22.0 40.0 43 33.058684046502805 37.0 33.0 39.0 20.0 40.0 44 32.96772445191187 37.0 32.0 39.0 19.0 40.0 45 32.73702609685375 37.0 32.0 39.0 18.0 40.0 46 32.36337768624156 36.0 32.0 39.0 16.0 40.0 47 32.21433023495298 36.0 31.0 39.0 16.0 40.0 48 32.04033766022601 36.0 31.0 39.0 14.0 40.0 49 32.080729519525214 36.0 31.0 39.0 15.0 40.0 50 31.935313406140754 36.0 31.0 39.0 11.0 40.0 51 31.610877753990408 36.0 31.0 39.0 2.0 40.0 52 31.475623966830167 36.0 31.0 39.0 2.0 40.0 53 31.23395029944988 36.0 30.0 39.0 2.0 40.0 54 31.226579225495243 36.0 30.0 39.0 2.0 40.0 55 30.99359095959459 36.0 30.0 39.0 2.0 40.0 56 30.68812498306279 36.0 30.0 39.0 2.0 40.0 57 30.357754532397497 36.0 30.0 39.0 2.0 40.0 58 29.879691607273514 35.0 29.0 38.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1 1 -1.626605646693804 1 2 -3.549344455882675 1 3 -4.53832101742708 1 4 -4.309001668771863 1 5 -2.658984733035574 1 6 -3.019904808351015 1 7 -3.098905896482968 1 8 -3.085571793937973 1 9 -3.072972211798259 1 10 -3.960935876013721 1 11 -1.6706093885640456 1 12 -2.0061644856577416 1 13 -3.0786044088092623 1 14 -3.2966348079561243 1 15 -2.646232292577416 1 16 -3.1673411677404957 1 17 -3.7394557512185997 1 18 -3.6680552791087564 1 19 -3.666246810953197 1 20 -3.26356351944721 1 21 -2.482256337195686 1 22 -3.6348716697156007 1 23 -2.5836583053690845 1 24 -2.6106039953269473 1 25 -3.5114976735829018 1 26 -2.94918275265654 1 27 -2.8953695726962323 1 28 -2.099934722182617 1 29 -2.4632739966296384 1 30 -3.167203213882413 1 31 -1.8501850179114783 1 32 -1.698409513327995 1 33 -2.8780817089996233 1 34 -3.2057639300233767 1 35 -3.8434565813704253 1 36 -0.8655156232465089 1 37 -1.118867204974329 1 38 -0.7691807550854435 1 39 -1.1035947763813354 1 40 -0.24388716426006596 1 41 -0.2748363568579677 1 42 -1.0824425617651414 1 43 -0.1619881487997077 1 44 -0.9582560591557225 1 45 -1.0065844835123272 1 46 -2.155927902025411 1 47 -3.008731540204618 1 48 -3.2673840326424113 1 49 -2.393286022448752 1 50 -2.4958201549699766 1 51 -1.977623247193634 1 52 -2.9024681523657705 1 53 -1.821242296276683 1 54 -1.8037261526025326 1 55 -3.082552678439324 1 56 -2.6280271728691247 1 57 -1.9960626712849994 1 58 -1.5356745326150048 2 1 -1.2238470260041439 2 2 -1.1514134213999156 2 3 -3.1383210174270815 2 4 -2.9945189101511716 2 5 -1.0948468020010864 2 6 -1.4868013600751482 2 7 -1.1658024482071028 2 8 -1.9752269663517694 2 9 -1.600558418694817 2 10 -1.9699013932550997 2 11 -1.4554369747709401 2 12 -1.1482334511749812 2 13 -0.3482595812230542 2 14 -2.248358945887155 2 15 -0.8379564305084486 2 16 -1.0638928918784245 2 17 -0.7794557512185989 2 18 -1.6218483825570331 2 19 -1.7959019833669885 2 20 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-0.544316639538966 117 58 -0.21503961198008525 118 1 -2.089938980027135 118 2 -1.98934445588268 118 3 -3.971654350760417 118 4 -3.712335002105192 118 5 -2.5523180663689047 118 6 -2.463238141684343 118 7 -2.7089058964829675 118 8 -2.76890512727131 118 9 -2.9729722117982647 118 10 -3.9176025426803847 118 11 -2.760609388564042 118 12 -2.9194978189910756 118 13 -2.625271075475929 118 14 -2.9633014746227886 118 15 -2.4028989592440837 118 16 -2.950674501073827 118 17 -3.2294557512186017 118 18 -3.2247219457754213 118 19 -3.772913477619859 118 20 -2.5935635194472084 118 21 -3.5355896705290206 118 22 -2.521538336382264 118 23 -2.7303249720357456 118 24 -3.3639373286602776 118 25 -2.754831006916234 118 26 -3.485849419323209 118 27 -2.4020362393629 118 28 -3.336601388849285 118 29 -3.1999406632963066 118 30 -2.9672032138824136 118 31 -3.006851684578148 118 32 -3.1184095133279968 118 33 -2.804748375666289 118 34 -4.029097263356711 118 35 -2.9734565813704243 118 36 -2.6988489565798446 118 37 -1.8022005383076625 118 38 -2.019180755085447 118 39 -2.260261443047998 118 40 -1.643887164260068 118 41 -2.0015030235246307 118 42 -1.559109228431808 118 43 -2.0153214821330394 118 44 -2.07825605915572 118 45 -0.9932511501789953 118 46 -1.5759279020254127 118 47 -1.2653982068712857 118 48 -2.400717365975744 118 49 -1.3266193557820856 118 50 -0.4924868216366427 118 51 -1.3242899138603015 118 52 -1.2791348190324392 118 53 -1.4745756296100154 118 54 -1.9470594859358634 118 55 -1.0458860117726552 118 56 -1.4213605062024612 118 57 -2.5427293379516627 118 58 -1.6023411992816712 119 1 -0.547875487963644 119 2 -1.1464873130255313 119 3 -2.60657498568105 119 4 -2.671858811629008 119 5 -1.5173974314482663 119 6 -0.32752385597006395 119 7 -1.4906519282289992 119 8 -1.1744606828268616 119 9 -1.2412261800522302 119 10 -1.7342692093470546 119 11 -0.293149071103727 119 12 -0.37346607295933154 119 13 -0.1506679008727545 119 14 -0.36330147462278717 119 15 -0.8497243560694798 119 16 -0.9689284693277997 119 17 -0.2635827353455795 119 18 -0.5413886124420912 119 19 -1.0586277633341439 119 20 -0.44038891627261023 119 21 -0.5689230038623521 119 22 -1.4215383363822625 119 23 -1.0874678291786068 119 24 -1.2401278048507578 119 25 -0.8048310069162383 119 26 -0.7501351336089215 119 27 -1.5687029060295714 119 28 -0.3802521825000795 119 29 -0.581686695042336 119 30 -1.2449809916601922 119 31 0.2590213312948677 119 32 0.24666985175137057 119 33 -0.6587166296345401 119 34 -0.5902083744678208 119 35 -1.3679010258148665 119 36 0.09797644024555296 119 37 0.10652962042249214 119 38 -0.6437839296886239 119 39 -1.1150233478099025 119 40 0.25452553415262713 119 41 -0.21737603939764938 119 42 -0.8868870062095837 119 43 -0.9010357678473255 119 44 -0.9560338369335 119 45 -0.4980130549408983 119 46 0.04788162178411426 119 47 -1.1900013814744597 119 48 -0.18325704851542568 119 49 -0.907571736734468 119 50 -0.3980423771921977 119 51 0.020154530584143515 119 52 -1.0275475174451358 119 53 0.553995798961413 119 54 -0.6970594859358634 119 55 0.07554255965591494 119 56 -0.5864398712818257 119 57 -1.1633642585865864 119 58 -1.3420237389642118 120 1 -2.121699986316443 120 2 -2.779910493618523 120 3 -5.072283281578024 120 4 -4.830888461224692 120 5 -3.5548337896393463 120 6 -2.8082066951434683 120 7 -4.968339858747122 120 8 -5.228968020353072 120 9 -5.235236362741659 120 10 -3.7028226684665526 120 11 -2.912496181016877 120 12 -2.0314475045256657 120 13 -2.356717616356427 120 14 -1.9985216004089565 120 15 -2.0503832359736407 120 16 -1.8937562620801174 120 17 -1.203984053105394 120 18 -1.32126282627857 120 19 -1.9741713392550793 120 20 -1.8360163496358908 120 21 -1.675841242856066 120 22 -1.8869471414137138 120 23 -2.639130003482286 120 24 -1.789094561364685 120 25 -2.245082579243281 120 26 -1.4257865262414455 120 27 -1.178765799111332 120 28 -1.8067271750128029 120 29 -2.6002551287051148 120 30 -2.5804107610522244 120 31 -2.429807659420913 120 32 -2.608975551063846 120 33 -1.8135534071128312 120 34 -1.6551978922875286 120 35 -1.961192430427026 120 36 -0.5692892081521705 120 37 -0.3415087144082918 120 38 -2.393709056972238 120 39 -0.14736836128699338 120 40 -0.7646418812412037 120 41 -1.0442703191221199 120 42 -0.706593505161365 120 43 -0.8102900355921605 120 44 -0.44240700255194554 120 45 -1.3039429740783675 120 46 -0.49479582655371246 120 47 0.5569288371538761 120 48 -0.016440636415993026 120 49 0.6746385058531317 120 50 -0.15695223044167506 120 51 0.6763390169573071 120 52 0.8469658098983821 120 53 0.3266822320252061 120 54 1.6740096964540712 120 55 0.7808435479757705 120 56 1.0553690535459666 120 57 1.1597863853187746 120 58 1.7699858447434842 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 163.0 3 9.0 4 18.0 5 9.0 6 30.0 7 46.0 8 51.0 9 74.0 10 96.0 11 120.0 12 132.0 13 186.0 14 197.0 15 234.0 16 245.0 17 285.0 18 327.0 19 335.0 20 398.0 21 492.0 22 563.0 23 793.0 24 957.0 25 866.0 26 893.0 27 1072.0 28 1334.0 29 1552.0 30 1911.0 31 2331.0 32 2875.0 33 3560.0 34 4568.0 35 6356.0 36 8915.0 37 12377.0 38 12419.0 39 7004.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 68.60925178179454 17.362673098290017 3.6069483211837077 10.421126798731741 11 42.36741551719466 16.163518603831875 14.296360532234898 27.172705346738567 12 42.5286577599523 9.590526002005365 28.110349313026745 19.77046692501558 13 22.584753800710008 13.59854746483835 32.31619739302458 31.50050134142706 14 17.45210156906317 16.542912116202814 46.1776103628628 19.827375951871222 15 25.920706755914473 12.137882442210238 41.19807051299423 20.74334028888106 16 28.97928212354846 12.593325293695207 35.00494573244265 23.42244685031368 17 29.675347551556868 13.04842687190049 28.171323270372078 29.104902306170565 18 31.248475651066364 15.09986179236335 26.151052816996827 27.500609739573452 19 29.263831113399547 21.070175200878037 26.858714651562988 22.80727903415943 20 32.68970189701897 21.71951219512195 24.84417344173442 20.746612466124663 21 33.58040081843928 21.38782368324774 24.969173024024716 20.06260247428827 22 33.16395663956639 18.59891598915989 28.40243902439024 19.83468834688347 23 39.142276422764226 18.082655826558266 25.894308943089435 16.880758807588077 24 40.89591067992737 19.061814042979865 20.369366683829707 19.672908593263056 25 30.79997832037072 19.93577409826292 19.10652827836644 30.15771930299992 26 26.441688690113168 17.500847055634612 25.818255743037206 30.239208511215015 27 23.36210517757693 16.196696432200977 25.806582745023643 34.634615645198444 28 24.229158478240244 26.357019909735307 21.86275971429733 27.55106189772712 29 30.251406112353457 21.336314969167177 23.81242800027106 24.599850918208308 30 26.733089331706655 17.12213636979802 30.07455605259591 26.07021824589942 31 33.9428524954591 14.768075473744139 25.67977878385339 25.609293246943366 32 40.55127180084832 13.646280812543196 26.830458173539494 18.97198921306899 33 43.384465282671144 11.36240446636674 26.35237682259201 18.900753428370102 34 30.610779092301225 14.961174126926046 37.60756731850768 16.820479462265045 35 26.046889822469172 26.33961241360618 33.99241089578533 13.621086868139315 36 27.70857336496103 31.929515418502202 26.190443917316163 14.171467299220602 37 33.08981987232485 25.105379433729553 25.45506295658774 16.349737737357856 38 28.47672243160435 27.511455763130066 26.66955885144112 17.342262953824463 39 33.458498827420726 22.75481570849544 24.001952039474574 19.784733424609254 40 29.225638522856677 20.946532183721057 25.036603221083453 24.79122607233881 41 25.27110556850838 25.42563574255815 26.70932060944532 22.59393807948815 42 25.641373325378314 20.660899278624505 26.860389434289743 26.837337961707437 43 24.176599004732267 17.268030752959362 26.98070482311625 31.574665419192126 44 24.466945901617123 15.448741409458744 29.059411979992678 31.02490070893145 45 30.654540032801552 17.764343901215828 26.837632324437156 24.743483741545468 46 27.251159133429137 22.827472140126353 26.96374827146769 22.95762045497682 47 25.142089770892962 20.24253604807314 29.84902537947125 24.76634880156265 48 26.628783769122272 17.1083324292069 34.55435608115439 21.70852772051644 49 27.669244335911003 17.22968389635056 31.549314882648215 23.55175688509022 50 21.17758784425451 15.664088997422333 32.96296296296296 30.195360195360195 51 18.69769839009372 17.180019259198982 36.63181022907597 27.49047212163134 52 18.373379617074363 18.560503335683677 36.40641102131583 26.659706025926127 53 15.83666824933867 19.94709353591535 41.8544393949671 22.361798819778876 54 20.07000217060994 17.482906446711528 38.11862383329716 24.328467549381376 55 19.983986755146628 17.3187314253145 31.737437066590672 30.9598447529482 56 18.976655808903367 20.375950054288815 27.62757871878393 33.01981541802388 57 23.499396094290717 22.017452196452563 27.189327832589193 27.293823876667528 58 23.384502626328434 25.860173459831426 27.840438671498568 22.914885242341573 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 15.5 9 26.5 10 22.0 11 26.5 12 31.0 13 69.5 14 108.0 15 180.5 16 300.5 17 348.0 18 369.5 19 391.0 20 437.0 21 483.0 22 599.5 23 894.0 24 1072.0 25 1205.5 26 1339.0 27 1341.5 28 1301.0 29 1258.0 30 1081.0 31 904.0 32 1019.0 33 1134.0 34 911.0 35 750.5 36 813.0 37 597.5 38 382.0 39 481.0 40 580.0 41 722.0 42 1012.0 43 1160.0 44 1703.0 45 2246.0 46 1735.0 47 1381.5 48 1539.0 49 1764.0 50 1989.0 51 2717.0 52 3445.0 53 3455.5 54 3936.0 55 4406.0 56 5021.0 57 5636.0 58 6632.0 59 7761.5 60 7895.0 61 7876.5 62 7858.0 63 6336.0 64 4814.0 65 3809.5 66 2301.5 67 1798.0 68 1451.5 69 1105.0 70 1025.0 71 945.0 72 706.0 73 364.5 74 262.0 75 198.5 76 135.0 77 140.0 78 95.0 79 45.0 80 30.0 81 15.0 82 11.5 83 8.0 84 6.0 85 2.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0013549768298962088 17 0.0 18 0.0 19 0.0013549768298962088 20 0.0027099536597924175 21 0.004064930489688627 22 0.0027099536597924175 23 0.0027099536597924175 24 0.0 25 0.0 26 0.023034606108235548 27 0.004064930489688627 28 0.025744559768027964 29 0.023034606108235548 30 0.04335925855667868 31 0.03793935123709384 32 0.01219479146906588 33 0.008129860979377254 34 0.014904745128858298 35 0.016259721958754508 36 0.03658437440719764 37 0.028454513427820384 38 0.05419907319584835 39 0.04471423538657489 40 0.05148911953605593 41 0.040649304896886264 42 0.07316874881439528 43 0.07181377198449906 44 0.039294328066990056 45 0.033874420747405216 46 0.05419907319584835 47 0.1097531232215929 48 0.08942847077314978 49 0.1246578683504512 50 0.1246578683504512 51 0.09620335492263082 52 0.07316874881439528 53 0.11788298420097017 54 0.12194791469065878 55 0.1531123817782716 56 0.16530717324733749 57 0.155822335438064 58 0.1693721037370261 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 73802.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.374651093466298 #Duplication Level Percentage of deduplicated Percentage of total 1 67.01019917765792 17.003604238367522 2 10.888022641106424 5.525595512316739 3 5.521439632616009 4.20313812633804 4 3.2680087574090884 3.316983279585919 5 2.173332621348855 2.7573778488387846 6 1.580605542799167 2.406438849895667 7 1.3937096171303465 2.4755426682203736 8 1.0626368345170076 2.157123113194764 9 0.8917605596198003 2.036530175334002 >10 5.489400331072783 25.83263326197122 >50 0.4165109200619427 7.688138532831089 >100 0.2456346451647354 12.475271672854396 >500 0.04805895231483954 8.029592693964933 >1k 0.010679767181075453 4.092030026286551 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 1544 2.0920842253597463 No Hit ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 1476 1.9999458009268043 No Hit ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 998 1.3522668762364163 No Hit ATTTGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 785 1.0636568114685239 No Hit ATTTGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 785 1.0636568114685239 No Hit ATTTGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 607 0.8224709357469988 No Hit ATTTGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 590 0.7994363296387632 No Hit ATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 564 0.7642069320614617 No Hit ATTTGCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 561 0.7601420015717731 No Hit ATTTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 520 0.7045879515460285 No Hit ATTTGCGGGGTGCTATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC 516 0.6991680442264437 No Hit ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 460 0.623289341752256 No Hit ATTTGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 426 0.5772201295357849 No Hit ATTTGCGGGAGTTTCCTTGATGTGACTTACCTCTTTTTGTTTATTTCTTTTGAGACAG 405 0.5487656161079646 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 389 0.5270859868296252 No Hit ATTTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 349 0.47288691363377683 No Hit ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA 336 0.45527221484512614 No Hit ATTTGCGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA 294 0.3983631879894854 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA 287 0.38887835018021194 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA 282 0.38210346603073087 No Hit ATTTGCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 275 0.3726186282214574 No Hit ATTTGCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT 274 0.3712636513915612 No Hit ATTTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 264 0.3577138830925991 No Hit ATTTGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA 258 0.34958402211322187 No Hit ATTTGCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT 244 0.33061434649467497 No Hit ATTTGCGGGGGGGGATGAAGTTAGGAAAGCCACTCGGGTCGTCTTTGGCCGTCCACTT 216 0.2926749952575811 No Hit ATTTGCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 212 0.28725508793799626 No Hit ATTTGCGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTG 182 0.24660578304111 No Hit ATTTGCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 182 0.24660578304111 No Hit ATTTGCGGGAAGATGGAAGTTACCACACCTGTTACAGTTGCCTGAGAATTACAACACT 179 0.24254085255142138 No Hit ATTTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA 178 0.24118587572152517 No Hit ATTTGCGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA 175 0.23712094523183655 No Hit ATTTGCGGGACTGGAGTCCTGGTGTATCTGGCAAAGTATCATGGCTTCGGGAACGTCC 170 0.23034606108235547 No Hit ATTTGCGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA 162 0.2195062464431858 No Hit ATTTGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAGGAGAACTGACTGCTCTTCTGAA 161 0.21815126961328962 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA 155 0.2100214086339124 No Hit ATTTGCGGGGGAGAATGGTTGTAGTTGGAGTAGTACAAAGTGACCAAGCCAAAATCAG 155 0.2100214086339124 No Hit ATTTGCGGGGAGTGCAGTGCTATTTGCGGGGGGGAATTCCTTCGGCAACATTAACTGG 152 0.20595647814422371 No Hit ATTTGCGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC 151 0.2046015013143275 No Hit ATTTGCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT 150 0.2032465244844313 No Hit ATTTGCGGGAGTTCATAATGTTGTTCCACATATAGGGTTGCAGATCCCTTTAGATCCT 150 0.2032465244844313 No Hit ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT 134 0.18156689520609198 No Hit ATTTGCGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCCGA 133 0.18021191837619577 No Hit ATTTGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 130 0.17614698788650712 No Hit ATTTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 130 0.17614698788650712 No Hit ATTTGCGGGGGAATTCCTTCGGCGACATTAACTGGTTGATGCTGAAAAACATCGAACT 127 0.1720820573968185 No Hit ATTTGCGGGGACGCTACTCAGAATACCCAGATGCTTACACCACATGAAACACTGTCTC 127 0.1720820573968185 No Hit ATTTGCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT 127 0.1720820573968185 No Hit ATTTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 126 0.17072708056692232 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCTA 122 0.16530717324733749 No Hit ATTTGCGGGGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGGGAT 118 0.15988726592775263 No Hit ATTTGCGGGGTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTAC 116 0.15717731226796022 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA 114 0.15446735860816782 No Hit ATTTGCGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA 111 0.15040242811847918 No Hit ATTTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC 109 0.14769247445868675 No Hit ATTTGCGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC 107 0.14498252079889434 No Hit ATTTGCGGGATCATAAGGGAGGAAAATGTCCCAAAAAGCAGAGAGAAATATCTCTCTG 103 0.13956261347930948 No Hit ATTTGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC 100 0.13549768298962087 No Hit ATTTGCGGGGTTCACTGATTCCCATTATTTTCAGGCTTCACCCTAGATGACACATGAG 100 0.13549768298962087 No Hit ATTTGCGGGGGGATTGGTGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTTTTCCAA 99 0.13414270615972468 No Hit ATTTGCGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAA 98 0.13278772932982846 No Hit ATTTGCGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 98 0.13278772932982846 No Hit ATTTGCGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA 97 0.13143275249993225 No Hit ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 97 0.13143275249993225 No Hit ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG 97 0.13143275249993225 No Hit ATTTGCGGGGGGCTGGAGAAATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAGA 96 0.13007777567003606 No Hit ATTTGCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG 95 0.12872279884013985 No Hit ATTTGCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA 95 0.12872279884013985 No Hit ATTTGCGGGCTAGATTCTAAGGATCATGTCTGCGAGTCAGGATTCTCGATCCAGAGAC 93 0.12601284518034742 No Hit ATTTGCGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA 93 0.12601284518034742 No Hit ATTTGCGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCT 93 0.12601284518034742 No Hit ATTTGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 92 0.1246578683504512 No Hit ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC 91 0.123302891520555 No Hit ATTTGCGGGCTTTTCCTCTGGCGCGGTGAGTGAGTTCGGTCACGGTTTTTTACGATGG 90 0.12194791469065878 No Hit ATTTGCGGGGTGTAGCCTGTTGTAGCTGGGGATGTTCTTCACGGGTAGAGTGTTTGCC 90 0.12194791469065878 No Hit ATTTGCGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCTT 88 0.11923796103086638 No Hit ATTTGCGGGGCACTTCTGGGGGTCTTCCATAGCCTGCTCACAGAAAATGCAACCCCAG 86 0.11652800737107397 No Hit ATTTGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG 86 0.11652800737107397 No Hit ATTTGCGGGGTAGATCATGCTCTTTACACAGAGTACCGCTTATTTAATAAGACGGGAT 85 0.11517303054117774 No Hit ATTTGCGGGGGGGCTGGAGAGATGGCTCAATGGTTAAGAGCACTGACTGCTCTTCCGG 85 0.11517303054117774 No Hit ATTTGCGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG 83 0.11246307688138532 No Hit ATTTGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 82 0.11110810005148912 No Hit ATTTGCGGGCAGGAATACCACGACGCTACTCAGACTACCCAGATTCTTACACCACATG 82 0.11110810005148912 No Hit ATTTGCGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA 81 0.1097531232215929 No Hit ATTTGCGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGT 76 0.10297823907211186 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCCGACTGCTCTTCCAA 76 0.10297823907211186 No Hit ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT 76 0.10297823907211186 No Hit ATTTGCGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT 76 0.10297823907211186 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA 75 0.10162326224221566 No Hit ATTTGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 74 0.10026828541231944 No Hit ATTTGCGGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGTCA 74 0.10026828541231944 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0027099536597924175 0.0 0.0 0.0 0.0 12 0.004064930489688626 0.0 0.0 0.0 0.0 13 0.006774884149481044 0.0 0.0 0.0 0.0 14 0.008129860979377252 0.0 0.0 0.0 0.0 15 0.008129860979377252 0.0 0.0 0.0 0.0 16 0.008129860979377252 0.0 0.0 0.0 0.0 17 0.008129860979377252 0.0 0.0 0.0 0.0 18 0.01083981463916967 0.0 0.0 0.0 0.0 19 0.01083981463916967 0.0 0.0 0.0 0.0 20 0.013549768298962088 0.0 0.0 0.0 0.0 21 0.014904745128858296 0.0 0.0 0.0 0.0 22 0.016259721958754504 0.0 0.0 0.0 0.0 23 0.017614698788650716 0.0 0.0 0.0 0.0 24 0.017614698788650716 0.0 0.0 0.0 0.0 25 0.017614698788650716 0.0 0.0 0.0 0.0 26 0.017614698788650716 0.0 0.0 0.0 0.0 27 0.017614698788650716 0.0 0.0 0.0 0.0 28 0.029809490257716592 0.0 0.0 0.0 0.0 29 0.029809490257716592 0.0 0.0 0.0 0.0 30 0.03522939757730143 0.0 0.0 0.0 0.0 31 0.03522939757730143 0.0 0.0 0.0 0.0 32 0.03522939757730143 0.0 0.0 0.0 0.0 33 0.03522939757730143 0.0 0.0 0.0 0.0 34 0.03522939757730143 0.0 0.0 0.0 0.0 35 0.04335925855667868 0.0 0.0 0.0 0.0 36 0.04471423538657489 0.0 0.0 0.0 0.0 37 0.04471423538657489 0.0 0.0 0.0 0.0 38 0.04471423538657489 0.0 0.0 0.0 0.0 39 0.04877916587626351 0.0 0.0 0.0 0.0 40 0.04877916587626351 0.0 0.0 0.0 0.0 41 0.051489119536055936 0.0 0.0 0.0 0.0 42 0.052844096365952144 0.0 0.0 0.0 0.0 43 0.052844096365952144 0.0 0.0 0.0 0.0 44 0.05419907319584835 0.0 0.0 0.0 0.0 45 0.05690902685564077 0.0 0.0 0.0 0.0 46 0.05690902685564077 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTGA 30 7.853687E-7 52.037315 47 TTTGAGA 30 7.853687E-7 52.037315 49 TTTTGAG 30 7.853687E-7 52.037315 48 TTGTCAG 15 0.007173123 52.037315 40 TTCACGG 15 0.007173123 52.037315 38 ATCTTCG 15 0.007173123 52.037315 49 ACATGTC 15 0.007173123 52.037315 37 GGGCAAC 15 0.007173123 52.037315 42 ACTTGTC 15 0.007173123 52.037315 38 GAAATAT 15 0.007173123 52.037315 45 TTATCTC 15 0.007173123 52.037315 47 TTATTTC 30 7.853687E-7 52.037315 41 TCGAACT 45 8.913048E-11 52.037315 52 TGTTTAT 30 7.853687E-7 52.037315 38 AATTACA 30 7.853687E-7 52.037315 47 CAATTAT 15 0.007173123 52.037315 44 TTTCAAA 15 0.007173123 52.037315 52 TACATTT 15 0.007173123 52.037315 39 GTTTATT 30 7.853687E-7 52.037315 39 ATTACAA 30 7.853687E-7 52.037315 48 >>END_MODULE