FastQCFastQC Report
Tue 31 May 2016
SRR212556_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212556_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73945
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA15622.112380823585097No Hit
ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT13571.8351477449455675No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG9341.2631009534113191No Hit
ATTTGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG7461.0088579349516533No Hit
ATTTGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC7431.004800865508148No Hit
ATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5600.7573196294543241No Hit
ATTTGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5580.7546149164919873No Hit
ATTTGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5570.7532625600108188No Hit
ATTTGCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT5080.6869970924335655No Hit
ATTTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT4750.6423693285550071No Hit
ATTTGCGGGGTGCTATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC4750.6423693285550071No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4460.6031509906011224No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA4280.5788085739400906No Hit
ATTTGCGGGAGTTTCCTTGATGTGACTTACCTCTTTTTGTTTATTTCTTTTGAGACAG3900.52741902765569No Hit
ATTTGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3790.5125431063628372No Hit
ATTTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3650.4936101156264791No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA3290.4449252823044154No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA2890.390831023057678No Hit
ATTTGCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2850.3854215971330043No Hit
ATTTGCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT2780.3759551017648252No Hit
ATTTGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2660.35972682399080397No Hit
ATTTGCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT2610.3529650415849618No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA2600.3516126851037934No Hit
ATTTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA2600.3516126851037934No Hit
ATTTGCGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA2390.3232131989992562No Hit
ATTTGCGGGGGGGGATGAAGTTAGGAAAGCCACTCGGGTCGTCTTTGGCCGTCCACTT2360.3191561295557509No Hit
ATTTGCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC2040.27588072215836096No Hit
ATTTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA1830.2474812360538238No Hit
ATTTGCGGGGAGTGCAGTGCTATTTGCGGGGGGGAATTCCTTCGGCAACATTAACTGG1790.24207181012915005No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTG1720.232605314760971No Hit
ATTTGCGGGAAGATGGAAGTTACCACACCTGTTACAGTTGCCTGAGAATTACAACACT1670.22584353235512883No Hit
ATTTGCGGGGGAGAATGGTTGTAGTTGGAGTAGTACAAAGTGACCAAGCCAAAATCAG1570.21231996754344445No Hit
ATTTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.20420582865643386No Hit
ATTTGCGGGGACGCTACTCAGAATACCCAGATGCTTACACCACATGAAACACTGTCTC1500.2028534721752654No Hit
ATTTGCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1460.19744404625059164No Hit
ATTTGCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1450.19609168976942323No Hit
ATTTGCGGGAGTTCATAATGTTGTTCCACATATAGGGTTGCAGATCCCTTTAGATCCT1450.19609168976942323No Hit
ATTTGCGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCCGA1440.1947393332882548No Hit
ATTTGCGGGACTGGAGTCCTGGTGTATCTGGCAAAGTATCATGGCTTCGGGAACGTCC1410.19068226384474948No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA1380.18662519440124417No Hit
ATTTGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAGGAGAACTGACTGCTCTTCTGAA1370.18527283792007573No Hit
ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1350.18256812495773886No Hit
ATTTGCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT1350.18256812495773886No Hit
ATTTGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1320.17851105551423355No Hit
ATTTGCGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA1290.17445398607072823No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA1290.17445398607072823No Hit
ATTTGCGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1270.1717492731083914No Hit
ATTTGCGGGGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGGGAT1260.17039691662722292No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCTA1240.16769220366488605No Hit
ATTTGCGGGGTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTAC1210.16363513422138076No Hit
ATTTGCGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1200.16228277774021233No Hit
ATTTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1190.1609304212590439No Hit
ATTTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1160.15687335181553858No Hit
ATTTGCGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT1130.15281628237203326No Hit
ATTTGCGGGGGAATTCCTTCGGCGACATTAACTGGTTGATGCTGAAAAACATCGAACT1100.14875921292852795No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG1090.14740685644735954No Hit
ATTTGCGGGCTAGATTCTAAGGATCATGTCTGCGAGTCAGGATTCTCGATCCAGAGAC1090.14740685644735954No Hit
ATTTGCGGGGTTCACTGATTCCCATTATTTTCAGGCTTCACCCTAGATGACACATGAG1070.14470214348502264No Hit
ATTTGCGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA1060.14334978700385423No Hit
ATTTGCGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCT1050.14199743052268576No Hit
ATTTGCGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACAAA1010.13658800459801204No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1000.1352356481168436No Hit
ATTTGCGGGGGGCTGGAGAAATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAGA1000.1352356481168436No Hit
ATTTGCGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA1000.1352356481168436No Hit
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC980.13253093515450673No Hit
ATTTGCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG930.12576915274866454No Hit
ATTTGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC930.12576915274866454No Hit
ATTTGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC930.12576915274866454No Hit
ATTTGCGGGGGTCTGAGCCGCTTACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTG920.1244167962674961No Hit
ATTTGCGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC910.12306443978632768No Hit
ATTTGCGGGGTAGATCATGCTCTTTACACAGAGTACCGCTTATTTAATAAGACGGGAT900.12171208330515924No Hit
ATTTGCGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA890.12035972682399079No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAA870.11765501386165395No Hit
ATTTGCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA870.11765501386165395No Hit
ATTTGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA860.1163026573804855No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAATGGTTAAGAGCACTGACTGCTCTTCCGG850.11495030089931707No Hit
ATTTGCGGGCTTTTCCTCTGGCGCGGTGAGTGAGTTCGGTCACGGTTTTTTACGATGG830.11224558793698018No Hit
ATTTGCGGGGGGATTGGTGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTTTTCCAA830.11224558793698018No Hit
ATTTGCGGGGTGTAGCCTGTTGTAGCTGGGGATGTTCTTCACGGGTAGAGTGTTTGCC810.1095408749746433No Hit
ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT810.1095408749746433No Hit
ATTTGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG800.10818851849347488No Hit
ATTTGCGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCTT800.10818851849347488No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA800.10818851849347488No Hit
ATTTGCGGGAGCCTTTGGTGATCCTCCAGGACCTAAGGTTTCGGTATTATCTTCGGGG790.10683616201230646No Hit
ATTTGCGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT790.10683616201230646No Hit
ATTTGCGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA770.10413144904996957No Hit
ATTTGCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC770.10413144904996957No Hit
ATTTGCGGGCCTTCTGATCAATGAGCAGCCGAGGATTTATTGAAGAGCCAGTGTAAGC770.10413144904996957No Hit
ATTTGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG770.10413144904996957No Hit
ATTTGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC760.10277909256880113No Hit
ATTTGCGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTG760.10277909256880113No Hit
ATTTGCGGGATCATAAGGGAGGAAAATGTCCCAAAAAGCAGAGAGAAATATCTCTCTG750.1014267360876327No Hit
ATTTGCGGGCAGGAATACCACGACGCTACTCAGACTACCCAGATTCTTACACCACATG750.1014267360876327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTTA150.007160277652.06097822
CCTCGCA150.007160277652.06097826
GTTTCTT150.007160277652.06097822
CCACCGC307.8292396E-752.06097826
TATCTGG150.007160277652.06097825
AAAGGTT150.007160277652.06097827
TGCCAGA150.007160277652.06097823
GAATTGC150.007160277652.06097822
TATCAGG307.8292396E-752.06097824
TCTTCTA150.007160277652.06097826
CCCTCGC150.007160277652.06097825
TGTATCT150.007160277652.06097823
GAAAGGT150.007160277652.06097826
CTTCTAT150.007160277652.06097827
AATTGCT150.007160277652.06097823
TTGCCGG150.007160277652.06097826
CCAACTT251.6286882E-552.06097424
TGACTTA550.052.06097423
CCAGTAG251.6286882E-552.06097426
CAACTTG251.6286882E-552.06097425
Produced by FastQC (version 0.11.5)