FastQCFastQC Report
Tue 31 May 2016
SRR212555_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212555_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73176
Sequences flagged as poor quality0
Sequence length58
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT13901.8995299005138295No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA13711.8735651033125615No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG8831.2066797857220946No Hit
ATTTGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG6860.9374658357931562No Hit
ATTTGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC6280.8582048759156007No Hit
ATTTGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5780.7898764622280529No Hit
ATTTGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5730.7830436208592981No Hit
ATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4880.6668853175904668No Hit
ATTTGCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT4740.6477533617579534No Hit
ATTTGCGGGGTGCTATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC4550.6217885645566853No Hit
ATTTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT4480.6122225866404285No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4020.5493604460478845No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA3630.49606428337159725No Hit
ATTTGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3300.4509675303378157No Hit
ATTTGCGGGAGTTTCCTTGATGTGACTTACCTCTTTTTGTTTATTTCTTTTGAGACAG3280.44823439379031377No Hit
ATTTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG3260.4455012572428118No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA2810.38400568492401876No Hit
ATTTGCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2750.37580627528151306No Hit
ATTTGCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGT2710.37034000218650925No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA2620.3580408877227506No Hit
ATTTGCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT2420.33070952224773154No Hit
ATTTGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2350.3211435443314748No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA2230.3047447250464633No Hit
ATTTGCGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA2170.29654531540395757No Hit
ATTTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA2170.29654531540395757No Hit
ATTTGCGGGGGGGGATGAAGTTAGGAAAGCCACTCGGGTCGTCTTTGGCCGTCCACTT2040.2787799278451951No Hit
ATTTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA1890.25828140373893077No Hit
ATTTGCGGGAAGATGGAAGTTACCACACCTGTTACAGTTGCCTGAGAATTACAACACT1750.23914944790641737No Hit
ATTTGCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1740.23778287963266645No Hit
ATTTGCGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1710.2336831748114136No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTG1660.22685033344265879No Hit
ATTTGCGGGGAGTGCAGTGCTATTTGCGGGGGGGAATTCCTTCGGCAACATTAACTGG1590.21728435552640207No Hit
ATTTGCGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1500.2049852410626435No Hit
ATTTGCGGGACTGGAGTCCTGGTGTATCTGGCAAAGTATCATGGCTTCGGGAACGTCC1400.19131955832513392No Hit
ATTTGCGGGGTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTAC1290.1762873073138734No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA1270.1735541707663715No Hit
ATTTGCGGGGGAGAATGGTTGTAGTTGGAGTAGTACAAAGTGACCAAGCCAAAATCAG1270.1735541707663715No Hit
ATTTGCGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA1260.17218760249262055No Hit
ATTTGCGGGAGTTCATAATGTTGTTCCACATATAGGGTTGCAGATCCCTTTAGATCCT1260.17218760249262055No Hit
ATTTGCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1250.17082103421886957No Hit
ATTTGCGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCCGA1240.16945446594511862No Hit
ATTTGCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1230.16808789767136764No Hit
ATTTGCGGGGACGCTACTCAGAATACCCAGATGCTTACACCACATGAAACACTGTCTC1220.1667213293976167No Hit
ATTTGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1150.15715535148136No Hit
ATTTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.15715535148136No Hit
ATTTGCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT1140.15578878320760906No Hit
ATTTGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAGGAGAACTGACTGCTCTTCTGAA1110.15168907838635617No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA1100.15032251011260522No Hit
ATTTGCGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC1090.14895594183885427No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCTA1090.14895594183885427No Hit
ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1070.14622280529135234No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG1020.13938996392259756No Hit
ATTTGCGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGATACAAA1010.1380233956488466No Hit
ATTTGCGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA990.1352902591013447No Hit
ATTTGCGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT980.13392369082759376No Hit
ATTTGCGGGGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGGGAT970.1325571225538428No Hit
ATTTGCGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCT960.13119055428009183No Hit
ATTTGCGGGGGAATTCCTTCGGCGACATTAACTGGTTGATGCTGAAAAACATCGAACT950.12982398600634087No Hit
ATTTGCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA920.12572428118508802No Hit
ATTTGCGGGGGGCTGGAGAAATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAGA910.12435771291133704No Hit
ATTTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC910.12435771291133704No Hit
ATTTGCGGGGTTCACTGATTCCCATTATTTTCAGGCTTCACCCTAGATGACACATGAG910.12435771291133704No Hit
ATTTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC900.12299114463758609No Hit
ATTTGCGGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGTCA880.12025800809008418No Hit
ATTTGCGGGCTAGATTCTAAGGATCATGTCTGCGAGTCAGGATTCTCGATCCAGAGAC870.11889143981633322No Hit
ATTTGCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG860.11752487154258226No Hit
ATTTGCGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCTT850.11615830326883131No Hit
ATTTGCGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA850.11615830326883131No Hit
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC840.11479173499508036No Hit
ATTTGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA790.10795889362632557No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAA790.10795889362632557No Hit
ATTTGCGGGGTGTAGCCTGTTGTAGCTGGGGATGTTCTTCACGGGTAGAGTGTTTGCC790.10795889362632557No Hit
ATTTGCGGGCAGGAATACCACGACGCTACTCAGACTACCCAGATTCTTACACCACATG780.10659232535257461No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAATGGTTAAGAGCACTGACTGCTCTTCCGG780.10659232535257461No Hit
ATTTGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC750.10249262053132174No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC750.10249262053132174No Hit
ATTTGCGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA750.10249262053132174No Hit
ATTTGCGGGCTTTTCCTCTGGCGCGGTGAGTGAGTTCGGTCACGGTTTTTTACGATGG740.10112605225757079No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC150.006952064652.4498310
ACTGACA150.006952064652.4498310
GTACCTG150.006952064652.449839
GGCGTAG150.006952064652.4498310
GGGTACA150.006952064652.449837
GGATTCA307.435174E-752.449838
CGGGTTA150.006952064652.449836
GGGAGAC150.006952064652.449837
CGGGTGG600.052.449836
CGGGTCA150.006952064652.449836
CGGGCAA150.006952064652.449836
CGGGTAC503.6379788E-1252.449836
CGGGATC650.052.449836
CGGGACT900.052.449836
CGGGAAA251.5581849E-552.449836
GGTAGGT150.006952064652.449838
GACTGAC150.006952064652.449839
GGGACCT150.006952064652.449838
GGGCGTA150.006952064652.449839
TACCTGG150.006952064652.4498310
Produced by FastQC (version 0.11.5)