##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212554_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 73953 Sequences flagged as poor quality 0 Sequence length 56 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.78368693629738 39.0 38.0 40.0 34.0 40.0 2 37.81059591902965 39.0 38.0 40.0 35.0 40.0 3 37.908211972469 39.0 38.0 40.0 35.0 40.0 4 37.88874014576826 39.0 38.0 40.0 35.0 40.0 5 37.30906116046678 39.0 37.0 39.0 33.0 40.0 6 37.45097561965032 39.0 38.0 39.0 33.0 40.0 7 38.11304477167931 39.0 38.0 40.0 35.0 40.0 8 38.042445877787245 39.0 38.0 40.0 35.0 40.0 9 38.02223033548335 39.0 38.0 40.0 35.0 40.0 10 36.582221140454074 39.0 35.0 39.0 31.0 40.0 11 36.333590253268966 39.0 35.0 39.0 31.0 40.0 12 36.54406176896137 38.0 35.0 39.0 31.0 40.0 13 36.51911349100104 38.0 35.0 39.0 31.0 40.0 14 36.677606047083955 38.0 35.0 39.0 31.0 40.0 15 36.78179384203481 38.0 36.0 39.0 32.0 40.0 16 36.66005435884954 38.0 36.0 39.0 32.0 40.0 17 36.10739253309534 38.0 35.0 39.0 30.0 40.0 18 36.00914094086785 38.0 35.0 39.0 30.0 40.0 19 35.88104606980109 38.0 35.0 39.0 30.0 40.0 20 35.71184400903277 38.0 35.0 39.0 29.0 40.0 21 35.51048639000446 38.0 35.0 39.0 29.0 40.0 22 35.33100753181075 38.0 34.0 39.0 29.0 40.0 23 35.17025678471462 38.0 34.0 39.0 28.0 40.0 24 35.01388719862616 38.0 33.0 39.0 28.0 40.0 25 34.944275418171 38.0 33.0 39.0 28.0 40.0 26 34.34045948102173 38.0 33.0 39.0 27.0 40.0 27 34.13499114302327 37.0 33.0 38.0 26.0 40.0 28 33.71671196570795 36.0 33.0 38.0 25.0 40.0 29 33.5317837004584 36.0 33.0 38.0 24.0 40.0 30 33.14891890795505 36.0 32.0 38.0 23.0 40.0 31 32.438765161656725 36.0 31.0 38.0 20.0 39.0 32 32.45647911511365 36.0 31.0 38.0 20.0 39.0 33 32.01165605181669 36.0 30.0 38.0 18.0 39.0 34 31.90794153043149 35.0 30.0 38.0 18.0 39.0 35 31.4302191932714 35.0 30.0 38.0 16.0 39.0 36 31.659107811718254 36.0 30.0 38.0 12.0 40.0 37 31.291266074398603 35.0 30.0 38.0 7.0 40.0 38 30.830216488851026 35.0 30.0 38.0 2.0 39.0 39 30.658526361337607 35.0 29.0 38.0 2.0 39.0 40 30.581639690073423 35.0 29.0 38.0 2.0 39.0 41 29.90076129433559 35.0 28.0 38.0 2.0 39.0 42 29.58339756331724 35.0 27.0 38.0 2.0 39.0 43 29.482265763390263 35.0 27.0 38.0 2.0 39.0 44 29.388598163698564 35.0 27.0 38.0 2.0 39.0 45 28.71592768379917 35.0 26.0 38.0 2.0 39.0 46 27.98112314578178 34.0 25.0 38.0 2.0 39.0 47 27.680445688477818 33.0 23.0 38.0 2.0 39.0 48 27.33153489378389 33.0 23.0 38.0 2.0 39.0 49 27.19786891674442 33.0 23.0 38.0 2.0 39.0 50 26.7947209714278 33.0 21.0 38.0 2.0 39.0 51 25.942368801806552 33.0 2.0 37.0 2.0 39.0 52 25.676875853582683 33.0 2.0 37.0 2.0 39.0 53 25.324260002974864 32.0 2.0 36.0 2.0 39.0 54 25.07100455694833 32.0 2.0 36.0 2.0 39.0 55 24.606155260773736 32.0 2.0 37.0 2.0 39.0 56 14.311251740970617 18.0 2.0 23.0 2.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.9181154795067101 1 2 -2.3831273877842847 1 3 -2.8498173215474196 1 4 -3.0146089803990392 1 5 -2.3280311485241114 1 6 -2.072087206249037 1 7 -2.1820416235682245 1 8 -2.1636114920389886 1 9 -2.1018802309240456 1 10 -2.215221424146371 1 11 -2.7468392830571986 1 12 -3.2091272111135183 1 13 -2.7724434086399086 1 14 -1.1547556021408099 1 15 -2.2927857207450586 1 16 -3.186411403538848 1 17 -2.5776048544980696 1 18 -4.199497280262097 1 19 -4.230091208187126 1 20 -4.210425420028322 1 21 -3.892979797253936 1 22 -3.1524030008838153 1 23 -4.74234806326524 1 24 -3.4727594211114017 1 25 -5.084003290852333 1 26 -4.103137266008602 1 27 -4.4838043123769395 1 28 -3.4994197973182857 1 29 -3.888500159235697 1 30 -4.604409547898086 1 31 -4.446447679780878 1 32 -4.629371076117202 1 33 -4.715695700712065 1 34 -5.363932628370208 1 35 -4.08116313354418 1 36 -2.5244467630863667 1 37 -4.073144322235002 1 38 -2.8348348054295656 1 39 -2.8024358942279513 1 40 -1.9584499511454503 1 41 -2.732622056027047 1 42 -2.672017894866787 1 43 -2.285651919323872 1 44 -1.5859050818732072 1 45 -2.599889771672423 1 46 -3.2109061612832015 1 47 -2.1779991335038247 1 48 -2.414593561706095 1 49 -1.9029371556578276 1 50 -3.009266617266878 1 51 -2.716394234437569 1 52 -2.464340564332847 1 53 -2.2103183928267676 1 54 -2.4205060108632352 1 55 -2.3814139149248135 1 56 -0.8491333743028484 2 1 0.1266512291944224 2 2 -0.19397228059639104 2 3 -0.3772448120896641 2 4 0.08280589980272879 2 5 -0.7722303919036833 2 6 -0.5279510145718618 2 7 -0.16501766392131856 2 8 -0.08889522469977607 2 9 -0.15043004176894215 2 10 0.12998664647152935 2 11 -0.7663853107999543 2 12 -1.1753945503316814 2 13 -1.2544736734570563 2 14 -0.16515913303614127 2 15 -0.1534414584499757 2 16 -0.503246208078572 2 17 -0.3783614749268196 2 18 -0.2887154391523836 2 19 -0.16830053983908044 2 20 -1.628458206913571 2 21 -1.0736229246183768 2 22 -0.33273086973627386 2 23 0.642897838374104 2 24 -0.46519321682388437 2 25 -0.18173342956607996 2 26 0.14938480208723348 2 27 0.3547833294893863 2 28 -0.22766695999168007 2 29 -1.015549339563563 2 30 -1.0672090434844677 2 31 -1.351554867674949 2 32 -0.31789566628113874 2 33 0.2218831139159292 2 34 -1.3128607494294755 2 35 -1.4913144576299295 2 36 -1.1921642914596333 2 37 0.05674218470068482 2 38 -1.075692308582152 2 39 -0.6221080253754927 2 40 -0.6920565085224979 2 41 -0.20992344316449874 2 42 -0.2955992315628784 2 43 -0.3915787793490928 2 44 -0.3507222319362562 2 45 -0.3558796834000404 2 46 0.9864456672161701 2 47 0.31222785262479746 2 48 0.27109370185002035 2 49 0.5400010536738229 2 50 -0.164689063672931 2 51 0.025093785738977914 2 52 0.21945514815138978 2 53 0.017928769846626835 2 54 1.4546516183927558 2 55 1.2232519110524862 2 56 0.7050910897576816 3 1 0.4487953874041608 3 2 0.3274953228384234 3 3 0.43253921080911795 3 4 0.1535839377938828 3 5 0.6930311225381587 3 6 -0.36818000234183046 3 7 0.3936604521338509 3 8 0.26343368500618425 3 9 0.10874247969866246 3 10 0.47372851480356815 3 11 0.1524281162101957 3 12 0.05125129926499028 3 13 0.30966892347242236 3 14 0.2191760217908154 3 15 -0.6658015937609321 3 16 -0.09575206287951232 3 17 0.2807590038657892 3 18 0.5767542459894273 3 19 0.42680744871152854 3 20 0.3848126752097727 3 21 0.4699628156886746 3 22 0.9269620784812673 3 23 0.6465408256236458 3 24 1.1658242174722346 3 25 0.11074640389736601 3 26 -1.1217575346288697 3 27 0.08884526027263462 3 28 0.167857369958881 3 29 0.12340460266906206 3 30 -0.3602093036978431 3 31 0.49830201496882154 3 32 1.1166606699145447 3 33 0.40090991589354985 3 34 1.2520624876249116 3 35 1.4881286857476397 3 36 1.1721344334948256 3 37 1.711959462868787 3 38 2.2787183081235476 3 39 1.356294264502207 3 40 2.259804017108518 3 41 1.683128859723869 3 42 2.2760895532406593 3 43 1.9487803151083654 3 44 2.801762805794681 3 45 0.7206230488403982 3 46 3.682256231879194 3 47 3.4462377407330465 3 48 3.724905827793293 3 49 3.1785646758440045 3 50 2.9849336269333655 3 51 2.245754727711393 3 52 2.6464652964730107 3 53 2.098594916086544 3 54 3.304768714411491 3 55 1.510527477016577 3 56 0.3675943424248693 4 1 -0.1702522316434596 4 2 -0.6566316612885572 4 3 -0.7896830114131035 4 4 -0.6241938399838958 4 5 -0.43395300444596785 4 6 -0.4792911134529376 4 7 -0.3206252621518644 4 8 -0.11751869594619535 4 9 -0.17697180601562224 4 10 -0.47865243757738796 4 11 -0.8634448996628166 4 12 0.2734735214872117 4 13 -1.356997743194242 4 14 -0.06653826392346929 4 15 -0.4594523874117229 4 16 -0.22273618986363886 4 17 0.3442510673578525 4 18 0.005325674560857863 4 19 -0.09700207509799696 4 20 0.16259045298755126 4 21 -0.7205133747875152 4 22 0.5460096975288806 4 23 0.24971542879825392 4 24 0.2769353285833489 4 25 0.09487338802434664 4 26 0.35443294156160476 4 27 0.3428135142408877 4 28 -0.37182516972365676 4 29 0.45673793600239776 4 30 0.08423514074659977 4 31 -0.23185671518991313 4 32 -1.2325456792918068 4 33 -0.8848043698207348 4 34 -0.573334337771918 4 35 -1.0356808380618823 4 36 -0.8437385823781867 4 37 0.17227692318624221 4 38 -0.5784245490193101 4 39 0.27692918513712783 4 40 0.33916909647359716 4 41 0.27043044702545416 4 42 -0.7397834626323565 4 43 -0.0036006372725871927 4 44 0.2620802661121431 4 45 -0.04128171306436457 4 46 2.571145120768083 4 47 0.8589361534314612 4 48 1.994747097634562 4 49 1.8134853107646371 4 50 2.1754098174095553 4 51 2.340992822949488 4 52 0.7893224393301566 4 53 1.0668488843405122 4 54 1.38413379377657 4 55 0.30417827066737146 4 56 0.19299116782169357 5 1 -0.2924744538656867 5 2 -0.5510761057330029 5 3 0.13095190922181388 5 4 -0.4710192368092905 5 5 0.36684064634768276 5 6 0.038169204007374447 5 7 -0.1333236748502813 5 8 -0.4905345689620688 5 9 -0.03649561553942959 5 10 -1.6921445010694498 5 11 -1.4570956933136117 5 12 -0.05271695470326421 5 13 0.3160181298216287 5 14 -0.5752684226536289 5 15 -0.6094523874117286 5 16 0.38281936569191544 5 17 0.5506002737070546 5 18 0.5235796428148234 5 19 -0.25573223382815513 5 20 -0.010425420028319365 5 21 0.16599456172042437 5 22 -0.5524030008838139 5 23 -1.292348063265237 5 24 -1.4048107031626884 5 25 0.34804799119895335 5 26 -0.5415988044701407 5 27 -0.35559918417181535 5 28 -0.6019838998823914 5 29 -0.03850015923569572 5 30 -0.4967172402057827 5 31 0.1689369356037389 5 32 0.5706289238828006 5 33 0.8612273762110121 5 34 1.3488878844503063 5 35 -0.10552210790315186 5 36 1.0499122112726056 5 37 -0.603913553004233 5 38 -0.6284245490193072 5 39 -1.0524358942279513 5 40 -1.5584499511454517 5 41 -0.662109235514226 5 42 -0.28740251025140395 5 43 -1.13693397060592 5 44 0.285889789921665 5 45 -0.012710284492936097 5 46 -0.1788548792319169 5 47 -0.7164606719653648 5 48 -0.25049099760353144 5 49 -0.5285781812988546 5 50 -0.26824097624123766 5 51 -0.4574198754632093 5 52 -1.4297251797174653 5 53 -0.8910876235959968 5 54 -1.383326523683749 5 55 -0.2775677610786609 5 56 0.8072768821074074 6 1 -0.2521235766727017 6 2 0.021730911810855957 6 3 0.253758926765677 6 4 0.6807351491556233 6 5 0.16420906740031427 6 6 -0.003936059150518645 6 7 0.16141316725498456 6 8 0.13227244858179432 6 9 -0.03912719448680235 6 10 0.15960988489545969 6 11 -0.8851658687522104 6 12 -0.799208182773441 6 13 0.25286023508478905 6 14 0.21332806857444098 6 15 -0.582259404955586 6 16 -0.49174203781685577 6 17 -2.361680428047329 6 18 -1.219402813325523 6 19 -0.4136269706702578 6 20 -0.6577938410809523 6 21 -0.5910229821392221 6 22 -1.0997714219364454 6 23 -0.24234806326523994 6 24 -0.49165280842584025 6 25 -0.2668642895028057 6 26 -0.5670374009613681 6 27 -0.8117395350490071 6 28 -0.6458435490051926 6 29 0.29132440216780964 6 30 0.35240556681176116 6 31 1.0329720233230404 6 32 0.21273418704069513 6 33 -0.06859718519249469 6 34 0.9295896388362692 6 35 0.7295656113950955 6 36 1.7218420358340083 6 37 1.239946096118576 6 38 1.2461368544894604 6 39 2.1273886671755555 6 40 0.870497417275601 6 41 1.1142065539594554 6 42 0.26522906869596596 6 43 0.5578028714993408 6 44 0.9788722460620178 6 45 0.9837809435772407 6 46 2.0799170505926448 6 47 1.700205994701303 6 48 0.6714388269578713 6 49 0.2863340994029002 6 50 0.767723936039463 6 51 1.782931001729775 6 52 0.9772923641421869 6 53 1.1904913237724237 6 54 0.43509452894782896 6 55 -0.569673024236554 6 56 -0.3207932933311888 7 1 -0.6580111770295218 7 2 -0.6152003995183151 7 3 -0.30972605687988164 7 4 -0.8571774288996892 7 5 -0.5063231954602259 7 6 -0.8680454852581576 7 7 -0.10055531326835876 7 8 -0.058331179131563715 7 9 0.08130099463006246 7 10 -0.20316145022199095 7 11 -0.8152877837090955 7 12 -0.7224909660027024 7 13 -0.26364288712752426 7 14 -0.2874153153089978 7 15 -1.2673619919315016 7 16 -0.006163685155541998 7 17 -0.573693511603679 7 18 -0.43320001820212184 7 19 -0.22748364625753226 7 20 -0.8290694878249312 7 21 -0.552367020200478 7 22 -0.3557928313922929 7 23 -0.25929721580761367 7 24 -1.3762796297163575 7 25 -0.4601440991965333 7 26 0.6422995006146053 7 27 0.3675646576360947 7 28 0.8245697724339962 7 29 0.29370323059480796 7 30 1.127011573353542 7 31 1.8765640542478081 7 32 0.9130018052387285 7 33 0.929023986380507 7 34 1.9652720652412654 7 35 2.2933479485940182 7 36 2.4357879174872963 7 37 2.0181203453008543 7 38 2.3255302532405757 7 39 3.07892003797544 7 40 1.3593466590240446 7 41 2.3110546062936805 7 42 2.3651398626299525 7 43 2.499224221484475 7 44 2.249166626079859 7 45 2.2234479075974605 7 46 2.63329201342345 7 47 3.2369291585431093 7 48 3.176345160588557 7 49 3.231026338475157 7 50 3.455205351442377 7 51 3.196252440920972 7 52 5.271969735536775 7 53 3.321624240810781 7 54 4.784187600610036 7 55 3.560567832141679 7 56 3.3197062606384833 8 1 0.16892905490624344 8 2 0.5305028416354176 8 3 0.5344606811516357 8 4 0.5579281316117601 8 5 0.6729809972248759 8 6 0.5750113092705362 8 7 0.19650088655323117 8 8 0.3427987643712669 8 9 0.5924517528816224 8 10 -0.050916430894012876 8 11 -0.07814832489255963 8 12 -0.39569941084361915 8 13 -0.36117485263450533 8 14 0.2835035071709342 8 15 0.15458270030757149 8 16 1.0170298920077059 8 17 -0.7301014806789112 8 18 0.37708841474464805 8 19 -0.606609426810607 8 20 -0.13147805160726733 8 21 0.4089770178607779 8 22 -0.16994686053293862 8 23 0.5646694805944108 8 24 0.9118559635039816 8 25 0.8559427280410574 8 26 0.5557696165824879 8 27 1.8900148509159038 8 28 1.2664371527491909 8 29 0.8351840512906179 8 30 0.80854591768896 8 31 1.9803404443756705 8 32 0.8794008537073594 8 33 1.2296484288425944 8 34 0.9997650774327624 8 35 1.062898944728424 8 36 1.9499122112726042 8 37 2.7487180259431376 8 38 1.7373649246648988 8 39 1.9870377899825762 8 40 1.9055851365738512 8 41 2.5352591855384077 8 42 1.3880360862398256 8 43 2.2595572574642553 8 44 2.470100316237456 8 45 2.3522019962088194 8 46 3.027285471645275 8 47 2.1388024859293715 8 48 3.1451230374841863 8 49 2.33896567835027 8 50 2.487022181653497 8 51 1.344334510501703 8 52 0.6088713115106046 8 53 2.208035183421547 8 54 2.9087787394741476 8 55 2.9215550459388844 8 56 1.3107856540372325 9 1 0.02419221280098327 9 2 0.02913222760032852 9 3 -0.6034230907781861 9 4 -0.43768590347595904 9 5 -0.13211768698565152 9 6 0.270460870674043 9 7 -0.05103200818361131 9 8 -0.17803456896206882 9 9 -0.37712061553943244 9 10 0.12973049893054878 9 11 0.1606126400197212 9 12 0.9483247119634015 9 13 0.45351812982163153 9 14 0.6601482440130368 9 15 0.3009642792549414 9 16 0.4578193656919183 9 17 0.10268360704039026 9 18 0.45795464281482623 9 19 0.15572609950518057 9 20 0.1801995799716778 9 21 0.7055778950537572 9 22 1.1600969991161847 9 23 -0.16422306326523994 9 24 1.0837309635039816 9 25 0.7345063245322834 9 26 1.1396511955298578 9 27 1.5402341491615203 9 28 0.9344744334509443 9 29 1.8614998407643029 9 30 1.3126577597942202 9 31 2.075186935603739 9 32 1.5112539238827978 9 33 2.642477376211012 9 34 2.185346217783639 9 35 2.720519558763513 9 36 1.7988705446059399 9 37 2.1023364469957677 9 38 1.4944921176473578 9 39 0.26006410577204875 9 40 0.9634250488545497 9 41 2.155599097819106 9 42 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682.0 19 729.0 20 841.0 21 906.0 22 963.0 23 1210.0 24 1457.0 25 1592.0 26 1459.0 27 1700.0 28 1816.0 29 2093.0 30 2451.0 31 2865.0 32 3668.0 33 4550.0 34 5638.0 35 7028.0 36 8652.0 37 9977.0 38 7656.0 39 2856.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 67.3076142955661 18.134490825253877 3.6766594999526725 10.881235379227347 11 40.594702040485174 16.363095479561345 14.728273362811514 28.313929117141967 12 40.90571038362203 9.374873230294916 29.18475247792517 20.534663908157885 13 21.496085351507038 12.702662501859288 32.392195042797454 33.40905710383622 14 16.97023785377199 16.434762619501576 46.84732194772355 19.747677579002882 15 25.632496315227236 12.344326802158127 42.20788879423418 19.81528808838046 16 28.842643300474624 12.29429502521872 34.83834327207821 24.024718402228444 17 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30 27.20793474431587 18.189208964526706 29.300018969675616 25.302837321481803 31 33.79718706810114 15.574103682827023 25.430205143492046 25.198504105579794 32 39.71535072856659 13.123686885801423 26.30430362588953 20.856658759742462 33 42.668854326277675 11.936756749427095 25.615957259278343 19.778431665016882 34 30.092492134100034 15.46598676358902 37.342682000650974 17.098839101659976 35 24.584248935188953 25.51341526274382 34.92308944412794 14.979246357939285 36 29.527505631394686 30.315900887453523 25.561104024750996 14.595489456400793 37 32.9333514320619 23.78580154744129 25.273517035428263 18.007329985068548 38 28.5386516060713 26.067772679138724 26.920361671508864 18.47321404328111 39 32.50648310320014 21.864689829334853 26.05053426201241 19.578292805452595 40 29.431819725350106 19.632985153692562 24.752448350334824 26.18274677062251 41 26.466712851651113 24.543244087642798 26.503389162829237 22.48665389787685 42 25.981811508502915 19.512526677813575 28.55647540203635 25.949186411647158 43 25.072046109510087 16.27970202816595 26.752215757707575 31.89603610461639 44 25.459809958810816 14.717996819053056 28.30634965947555 31.515843562660578 45 30.642945552971263 17.949973476965763 27.068456631438636 24.33862433862434 46 26.92286763705618 22.142565637328254 26.480750918242418 24.453815807373147 47 25.3592423252776 19.04256477247986 29.20068582625735 26.397507075985192 48 25.38902427402557 17.196030114494985 34.08982614733231 23.325119464147143 49 27.459863945578235 18.23809523809524 30.556462585034012 23.745578231292516 50 21.489628137420375 16.622202863831873 32.02618827244515 29.861980726302605 51 18.984877975744872 18.052511943813037 36.11727395227919 26.8453361281629 52 18.81731410753918 18.73425649823673 35.86590961698188 26.582519777242215 53 15.609211368495595 20.454576405060532 40.74301044517983 23.193201781264044 54 20.683217964789886 18.357224614378374 37.54973565160517 23.40982176922658 55 19.220499686674113 18.114323079857233 30.36808980192355 32.29708743154511 56 19.13803906611818 20.271337891955213 26.595744680851062 33.99487836107554 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 18.0 9 36.0 10 33.0 11 30.0 12 31.0 13 62.0 14 92.0 15 184.5 16 277.0 17 351.0 18 425.0 19 404.0 20 383.0 21 457.5 22 705.0 23 878.0 24 975.0 25 1072.0 26 1218.0 27 1364.0 28 1443.0 29 1432.0 30 1342.0 31 1193.0 32 1044.0 33 1163.5 34 1283.0 35 1173.5 36 1064.0 37 822.5 38 585.0 39 589.0 40 765.0 41 941.0 42 1083.5 43 1226.0 44 1511.5 45 1797.0 46 1538.5 47 1358.0 48 1436.0 49 1575.5 50 1715.0 51 2488.0 52 3261.0 53 3227.0 54 3738.0 55 4283.0 56 5185.0 57 6087.0 58 6922.0 59 7757.0 60 8238.5 61 8720.0 62 8102.0 63 5906.5 64 4329.0 65 3524.0 66 2719.0 67 2262.0 68 1805.0 69 1526.0 70 1247.0 71 1049.0 72 668.0 73 485.0 74 408.5 75 332.0 76 277.5 77 223.0 78 164.0 79 88.5 80 72.0 81 49.5 82 27.0 83 22.0 84 17.0 85 12.0 86 7.0 87 6.0 88 3.5 89 2.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.032453044501237274 26 0.04462293618920125 27 0.1068246048165727 28 0.11899449650453667 29 0.15279975119332548 30 0.20418373832028452 31 0.20418373832028452 32 0.24069341338417644 33 0.2785552986356199 34 0.293429610698687 35 0.31236055332440876 36 0.3502224385758522 37 0.38267548307708954 38 0.3989020053277082 39 0.40566305626546595 40 0.44893378226711567 41 0.4543426230173218 42 0.5273619731451057 43 0.5260097629575541 44 0.5273619731451057 45 0.5841548010222709 46 0.599029113085338 47 0.6287777372114721 48 0.6761050937757764 49 0.6125512149608535 50 0.6558219409625032 51 0.6544697307749516 52 0.689627195651292 53 0.7058537179019106 54 0.7639987559666275 55 0.7396589725906996 56 0.7288412910902871 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 73953.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.134004029586357 #Duplication Level Percentage of deduplicated Percentage of total 1 71.72088849001571 21.612375427636472 2 10.033654924837334 6.047083958730545 3 4.801435943459726 4.340594702040486 4 2.925734799192282 3.5265641691344505 5 2.005833520305138 3.022189769177721 6 1.404532196544761 2.5394507322218165 7 1.018622391743325 2.1486619880194175 8 0.8032308727843841 1.9363649885738239 9 0.7000224366165583 1.8985031033223805 >10 3.9712811308054743 22.231687693535083 >50 0.3589858649315683 7.341149108217381 >100 0.2198788422705856 13.335496869633415 >500 0.02243661655822302 3.8389247224588594 >1k 0.013461969934933812 6.180952767298149 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 2034 2.750395521479859 No Hit ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 1307 1.7673387151298798 No Hit ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 1230 1.6632185306884102 No Hit ATTTGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 625 0.8451313672197206 No Hit ATTTGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 594 0.8032128514056224 No Hit ATTTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 584 0.7896907495301069 No Hit ATTTGCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC 519 0.7017970873392559 No Hit ATTTGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 517 0.699092666964153 No Hit ATTTGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT 466 0.6301299473990237 No Hit ATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 406 0.5489973361459306 No Hit ATTTGCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 386 0.5219531323948995 No Hit ATTTGCGGGGTGCTATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGA 383 0.5178965018322448 No Hit ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 361 0.4881478777061106 No Hit ATTTGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 354 0.47868240639324977 No Hit ATTTGCGGGAGTTTCCTTGATGTGACTTACCTCTTTTTGTTTATTTCTTTTGAGAC 332 0.44893378226711567 No Hit ATTTGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 305 0.4124241072032237 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 291 0.39349316457750194 No Hit ATTTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 284 0.3840276932646411 No Hit ATTTGCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 284 0.3840276932646411 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 256 0.3461658080131976 No Hit ATTTGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA 252 0.3407569672629914 No Hit ATTTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 230 0.3110083431368572 No Hit ATTTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 228 0.30830392276175406 No Hit ATTTGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC 220 0.29748624126134166 No Hit ATTTGCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 218 0.29478182088623855 No Hit ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC 212 0.2866685597609292 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 195 0.26368098657255284 No Hit ATTTGCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 190 0.25691993563479504 No Hit ATTTGCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 187 0.25286330507214044 No Hit ATTTGCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT 183 0.2474544643219342 No Hit ATTTGCGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA 183 0.2474544643219342 No Hit ATTTGCGGGGGGGGATGAAGTTAGGAAAGCCACTCGGGTCGTCTTTGGCCGTCCAC 182 0.24610225413438264 No Hit ATTTGCGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC 166 0.22446689113355783 No Hit ATTTGCGGGAGTTCATAATGTTGTTCCACATATAGGGTTGCAGATCCCTTTAGATC 164 0.2217624707584547 No Hit ATTTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 164 0.2217624707584547 No Hit ATTTGCGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTC 156 0.21094478925804228 No Hit ATTTGCGGGAGGCTGGTTTTTGAACTCCTAGGCTCCTCTTACTACATAAGCCCCCT 156 0.21094478925804228 No Hit ATTTGCGGGAAGATGGAAGTTACCACACCTGTTACAGTTGCCTGAGAATTACAACA 155 0.20959257907049073 No Hit ATTTGCGGGGACTGGGAGCGACCGAGCGGGCCACCGCTGCCGCCATGAACCCCGAA 155 0.20959257907049073 No Hit ATTTGCGGGGCACTTCTGGGGGTCTTCCATAGCCTGCTCACAGAAAATGCAACCCC 155 0.20959257907049073 No Hit ATTTGCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAA 150 0.20283152813273297 No Hit ATTTGCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG 138 0.18660500588211432 No Hit ATTTGCGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCC 134 0.18119616513190812 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC 132 0.178491744756805 No Hit ATTTGCGGGACTGGAGTCCTGGTGTATCTGGCAAAGTATCATGGCTTCGGGAACGT 130 0.1757873243817019 No Hit ATTTGCGGGGAGTGCAGTGCTATTTGCGGGGGGGAATTCCTTCGGCAACATTAACT 123 0.16632185306884104 No Hit ATTTGCGGGGGAGAATGGTTGTAGTTGGAGTAGTACAAAGTGACCAAGCCAAAATC 122 0.16496964288128946 No Hit ATTTGCGGGGACGCTACTCAGAATACCCAGATGCTTACACCACATGAAACACTGTC 115 0.1555041715684286 No Hit ATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 110 0.14874312063067083 No Hit ATTTGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAGGAGAACTGACTGCTCTTCTG 110 0.14874312063067083 No Hit ATTTGCGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT 109 0.14739091044311928 No Hit ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC 108 0.14603870025556773 No Hit ATTTGCGGGGACTGTCCTTAGTCTCAATCTGGGGCGGGAACGTCTTTACTCTTTCC 107 0.14468649006801618 No Hit ATTTGCGGGGGGGCTGGAGAGATGGCTCAATGGTTAAGAGCACTGACTGCTCTTCC 106 0.1433342798804646 No Hit ATTTGCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC 106 0.1433342798804646 No Hit ATTTGCGGGCTAGATTCTAAGGATCATGTCTGCGAGTCAGGATTCTCGATCCAGAG 102 0.1379254391302584 No Hit ATTTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 101 0.13657322894270685 No Hit ATTTGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 97 0.13116438819250065 No Hit ATTTGCGGGGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGGG 94 0.127107757629846 No Hit ATTTGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCT 92 0.12440333725474287 No Hit ATTTGCGGGGTTCACTGATTCCCATTATTTTCAGGCTTCACCCTAGATGACACATG 91 0.12305112706719132 No Hit ATTTGCGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCT 88 0.11899449650453667 No Hit ATTTGCGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACA 88 0.11899449650453667 No Hit ATTTGCGGGGGAATTCCTTCGGCGACATTAACTGGTTGATGCTGAAAAACATCGAA 88 0.11899449650453667 No Hit ATTTGCGGGGGGCTGGAGAAATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA 87 0.1176422863169851 No Hit ATTTGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 86 0.11629007612943357 No Hit ATTTGCGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCC 86 0.11629007612943357 No Hit ATTTGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 85 0.114937865941882 No Hit ATTTGCGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTC 85 0.114937865941882 No Hit ATTTGCGGGCTCTTTTCCTCTGGCGCGGTGAGTGAGTTCGGTCACGGTTTTTTACG 84 0.11358565575433045 No Hit ATTTGCGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 83 0.11223344556677892 No Hit ATTTGCGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGC 81 0.10952902519167579 No Hit ATTTGCGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCC 79 0.1068246048165727 No Hit ATTTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 79 0.1068246048165727 No Hit ATTTGCGGGATCATAAGGGAGGAAAATGTCCCAAAAAGCAGAGAGAAATATCTCTC 78 0.10547239462902114 No Hit ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTT 77 0.10412018444146957 No Hit ATTTGCGGGGTAGATCATGCTCTTTACACAGAGTACCGCTTATTTAATAAGACGGG 77 0.10412018444146957 No Hit ATTTGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC 76 0.10276797425391802 No Hit ATTTGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 75 0.10141576406636649 No Hit ATTTGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 75 0.10141576406636649 No Hit ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 75 0.10141576406636649 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.001352210187551553 0.0 0.0 0.0 0.0 12 0.001352210187551553 0.0 0.0 0.0 0.0 13 0.001352210187551553 0.0 0.0 0.0 0.0 14 0.004056630562654659 0.0 0.0 0.0 0.0 15 0.004056630562654659 0.0 0.0 0.0 0.0 16 0.004056630562654659 0.0 0.0 0.0 0.0 17 0.004056630562654659 0.0 0.0 0.0 0.0 18 0.004056630562654659 0.0 0.0 0.0 0.0 19 0.004056630562654659 0.0 0.0 0.0 0.0 20 0.004056630562654659 0.0 0.0 0.0 0.0 21 0.004056630562654659 0.0 0.0 0.0 0.0 22 0.005408840750206212 0.0 0.0 0.0 0.0 23 0.005408840750206212 0.0 0.0 0.0 0.0 24 0.010817681500412424 0.0 0.0 0.0 0.0 25 0.01352210187551553 0.0 0.0 0.0 0.0 26 0.01352210187551553 0.0 0.0 0.0 0.0 27 0.01352210187551553 0.0 0.0 0.0 0.0 28 0.02163536300082485 0.0 0.0 0.0 0.0 29 0.02163536300082485 0.0 0.0 0.0 0.0 30 0.022987573188376402 0.0 0.0 0.0 0.0 31 0.022987573188376402 0.0 0.0 0.0 0.0 32 0.025691993563479506 0.0 0.0 0.0 0.0 33 0.025691993563479506 0.0 0.0 0.0 0.0 34 0.02704420375103106 0.0 0.0 0.0 0.0 35 0.032453044501237274 0.0 0.0 0.0 0.0 36 0.033805254688788824 0.0 0.0 0.0 0.0 37 0.033805254688788824 0.0 0.0 0.0 0.0 38 0.03515746487634038 0.0 0.0 0.0 0.0 39 0.03786188525144348 0.0 0.0 0.0 0.0 40 0.04056630562654659 0.0 0.0 0.0 0.0 41 0.041918515814098146 0.0 0.0 0.0 0.0 42 0.04462293618920125 0.0 0.0 0.0 0.0 43 0.045975146376752804 0.0 0.0 0.0 0.0 44 0.05138398712695901 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTCTT 20 4.1283626E-4 50.073223 40 TTACTCT 20 4.1283626E-4 50.073223 46 ATTATCT 40 2.590241E-9 50.073223 46 GCCGTCC 40 2.590241E-9 50.073223 48 TTTACTC 20 4.1283626E-4 50.073223 45 TGAACCC 20 4.1283626E-4 50.073223 46 CGTCCAC 40 2.590241E-9 50.073223 50 TGGCCGT 40 2.590241E-9 50.073223 46 TGAGAAT 30 1.0240838E-6 50.07322 43 ATCCCCT 15 0.008351507 50.07322 48 CATGAAA 15 0.008351507 50.07322 43 AACAACC 15 0.008351507 50.07322 50 CACATTT 15 0.008351507 50.07322 47 AATCCAC 15 0.008351507 50.07322 43 TAAACCC 15 0.008351507 50.07322 48 CACATGA 15 0.008351507 50.07322 41 TAGTGGA 15 0.008351507 50.07322 45 CTTTAGA 15 0.008351507 50.07322 48 ATCCACA 15 0.008351507 50.07322 44 ACAGAAA 15 0.008351507 50.07322 40 >>END_MODULE