FastQCFastQC Report
Tue 31 May 2016
SRR212553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212553_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69350
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC18822.713770728190339No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC12341.779379956741168No Hit
ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC12061.7390050468637348No Hit
ATTTGCGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC7011.010814708002884No Hit
ATTTGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA6910.996395097332372No Hit
ATTTGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA5500.7930785868781542No Hit
ATTTGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC5130.7397260273972602No Hit
ATTTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4960.71521268925739No Hit
ATTTGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT4940.7123287671232876No Hit
ATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC4220.608507570295602No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4080.5883201153568854No Hit
ATTTGCGGGGTGCTATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGA3880.5594808940158615No Hit
ATTTGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA3820.5508291276135544No Hit
ATTTGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA3390.4888248017303533No Hit
ATTTGCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3350.48305695746214855No Hit
ATTTGCGGGAGTTTCCTTGATGTGACTTACCTCTTTTTGTTTATTTCTTTTGAGAC2900.41816870944484497No Hit
ATTTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2860.4124008651766402No Hit
ATTTGCGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT2650.3821196827685652No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2610.37635183850036047No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC2440.35183850036049025No Hit
ATTTGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA2410.3475126171593367No Hit
ATTTGCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2290.33020908435472246No Hit
ATTTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG2200.3172314347512617No Hit
ATTTGCGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT2050.2956020187454939No Hit
ATTTGCGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG1900.273972602739726No Hit
ATTTGCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1870.26964671953857244No Hit
ATTTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC1830.26387887527036774No Hit
ATTTGCGGGGGGGGATGAAGTTAGGAAAGCCACTCGGGTCGTCTTTGGCCGTCCAC1800.2595529920692142No Hit
ATTTGCGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA1790.2581110310021629No Hit
ATTTGCGGGGACTGGGAGCGACCGAGCGGGCCACCGCTGCCGCCATGAACCCCGAA1770.25522710886806055No Hit
ATTTGCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1730.2494592645998558No Hit
ATTTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1710.2465753424657534No Hit
ATTTGCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG1680.24224945926459984No Hit
ATTTGCGGGAGGCTGGTTTTTGAACTCCTAGGCTCCTCTTACTACATAAGCCCCCT1600.23071377072819035No Hit
ATTTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1470.21196827685652486No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1450.20908435472242248No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTC1420.20475847152126891No Hit
ATTTGCGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC1410.20331651045421775No Hit
ATTTGCGGGGACTGTCCTTAGTCTCAATCTGGGGCGGGAACGTCTTTACTCTTTCC1400.20187454938716656No Hit
ATTTGCGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAA1370.197548666186013No Hit
ATTTGCGGGAAGATGGAAGTTACCACACCTGTTACAGTTGCCTGAGAATTACAACA1350.1946647440519106No Hit
ATTTGCGGGGCACTTCTGGGGGTCTTCCATAGCCTGCTCACAGAAAATGCAACCCC1300.18745493871665464No Hit
ATTTGCGGGGAGTGCAGTGCTATTTGCGGGGGGGAATTCCTTCGGCAACATTAACT1270.1831290555155011No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCC1260.1816870944484499No Hit
ATTTGCGGGAGTTCATAATGTTGTTCCACATATAGGGTTGCAGATCCCTTTAGATC1260.1816870944484499No Hit
ATTTGCGGGGGAGAATGGTTGTAGTTGGAGTAGTACAAAGTGACCAAGCCAAAATC1200.17303532804614274No Hit
ATTTGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAGGAGAACTGACTGCTCTTCTG1190.17159336697909156No Hit
ATTTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1140.16438356164383564No Hit
ATTTGCGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCC1120.16149963950973323No Hit
ATTTGCGGGCTAGATTCTAAGGATCATGTCTGCGAGTCAGGATTCTCGATCCAGAG1100.15861571737563085No Hit
ATTTGCGGGACTGGAGTCCTGGTGTATCTGGCAAAGTATCATGGCTTCGGGAACGT1070.15428983417447728No Hit
ATTTGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1070.15428983417447728No Hit
ATTTGCGGGGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGGG1040.14996395097332374No Hit
ATTTGCGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG1010.14563806777217014No Hit
ATTTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC1000.14419610670511895No Hit
ATTTGCGGGGTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCT970.13987022350396539No Hit
ATTTGCGGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT950.136986301369863No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC940.13554434030281182No Hit
ATTTGCGGGGACGCTACTCAGAATACCCAGATGCTTACACCACATGAAACACTGTC940.13554434030281182No Hit
ATTTGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC930.13410237923576063No Hit
ATTTGCGGGGGGCTGGAGAAATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCA910.13121845710165828No Hit
ATTTGCGGGGGAATTCCTTCGGCGACATTAACTGGTTGATGCTGAAAAACATCGAA910.13121845710165828No Hit
ATTTGCGGGCTCTTTTCCTCTGGCGCGGTGAGTGAGTTCGGTCACGGTTTTTTACG880.12689257390050468No Hit
ATTTGCGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT880.12689257390050468No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAATGGTTAAGAGCACTGACTGCTCTTCC880.12689257390050468No Hit
ATTTGCGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGGGAAAAAGCGCCTGATACA880.12689257390050468No Hit
ATTTGCGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC880.12689257390050468No Hit
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG850.12256669069935111No Hit
ATTTGCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACAC840.12112472963229992No Hit
ATTTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG820.11824080749819756No Hit
ATTTGCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC810.11679884643114637No Hit
ATTTGCGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCT800.11535688536409518No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAGCACCCGACTGCTCTTCC800.11535688536409518No Hit
ATTTGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCT800.11535688536409518No Hit
ATTTGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT780.1124729632299928No Hit
ATTTGCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG780.1124729632299928No Hit
ATTTGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA770.1110310021629416No Hit
ATTTGCGGGGGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGCT770.1110310021629416No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCCGACTGCTCTTCC770.1110310021629416No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC770.1110310021629416No Hit
ATTTGCGGGGTGTAGCCTGTTGTAGCTGGGGATGTTCTTCACGGGTAGAGTGTTTG770.1110310021629416No Hit
ATTTGCGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCC770.1110310021629416No Hit
ATTTGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGGGGGCCCAAGAAACA760.1095890410958904No Hit
ATTTGCGGGATCATAAGGGAGGAAAATGTCCCAAAAAGCAGAGAGAAATATCTCTC760.1095890410958904No Hit
ATTTGCGGGAGCCTTTGGTGATCCTCCAGGACCTAAGGTTTCGGTATTATCTTCGG750.10814708002883922No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCC740.10670511896178803No Hit
ATTTGCGGGGGCTCTCCGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGAC730.10526315789473684No Hit
ATTTGCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGG720.10382119682768565No Hit
ATTTGCGGGGGTCAGACGGGCTCTGCGGCGCTTCTGTGTTGTCATTCGGCAGCTAG720.10382119682768565No Hit
ATTTGCGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTC710.10237923576063446No Hit
ATTTGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.10093727469358328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGAA451.2369128E-1050.32629850
CTTTTGA309.880787E-750.32629447
CCCAGAA354.9329174E-850.32629447
CTTTAGA251.9888223E-550.32629448
AGCCAGT150.00818434550.32629450
TAAGTTT150.00818434550.32629449
TTGTAAG150.00818434550.32629446
CCAGAAA354.9329174E-850.32629448
ATGAACC309.880787E-750.32629445
CCTTTAG251.9888223E-550.32629447
GTAAGTT150.00818434550.32629448
TTTAGAT251.9888223E-550.32629449
TAGAGTG150.00818434550.32629446
ACCCAGC150.00818434550.32629446
TTGGCCG550.050.32629445
GCCGTCC550.050.32629448
CAGAAAC309.880787E-750.32629449
TGGGAAC150.00818434550.32629446
AATGCAA150.00818434550.32629446
CCCCAGA354.9329174E-850.32629446
Produced by FastQC (version 0.11.5)