FastQCFastQC Report
Tue 31 May 2016
SRR212551_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212551_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77967
Sequences flagged as poor quality0
Sequence length58
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA12821.64428540279862No Hit
GTCGGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG10701.372375492195416No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG8811.1299652417048238No Hit
GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC6650.8529249554298614No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT6550.8400990162504649No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5740.736208908897354No Hit
GTCGGTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC5210.668231431246553No Hit
GTCGGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC5180.6643836494927341No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4020.5156027550117358No Hit
GTCGGTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3960.5079071915040979No Hit
GTCGGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3770.4835379070632447No Hit
GTCGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT3640.46686418613002933No Hit
GTCGGTGGGATTGCAGATATCTGATGGATGTAGTTTTCTTTTTTTAAATTAGAAATGC3230.4142778354945041No Hit
GTCGGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2880.3693870483666166No Hit
GTCGGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2830.36297407877691845No Hit
GTCGGTGGGAGTCTTAACATACATGTAGCAGATTGTATGTCATAGGGTACTTCTGTTT2790.35784370310515984No Hit
GTCGGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2660.34116998217194455No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2640.3386047943360653No Hit
GTCGGTGGGGAGCCAGCCCCAGAGAGGTGAGAGGAAGTGGTGTCAAGGGCTTTGCCCT2520.3232136673207896No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA2480.318083291649031No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT2250.28858363153641925No Hit
GTCGGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT2210.2834532558646607No Hit
GTCGGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT2200.282170661946721No Hit
GTCGGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2180.2796054741108418No Hit
GTCGGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC2170.27832288019290213No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT2150.27575769235702285No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA2090.268062128849385No Hit
GTCGGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1830.2347146869829543No Hit
GTCGGTGGGATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAG1640.21034540254210116No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1630.20906280862416152No Hit
GTCGGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1590.20393243295240296No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGCTCTTCCGA1560.200084651198584No Hit
GTCGGTGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1540.19751946336270476No Hit
GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1530.19623686944476507No Hit
GTCGGTGGGGAATACCAATTTTAAAGTTTCTGAAGAATTGCTAAGAGTCAGAGGAATG1480.18982389985506687No Hit
GTCGGTGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1480.18982389985506687No Hit
GTCGGTGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG1410.1808457424294894No Hit
GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC1380.17699796067567047No Hit
GTCGGTGGGAATTTGGAAGGGTGGAAATGATGTAAATATAGTATTCATGTATGAAATT1380.17699796067567047No Hit
GTCGGTGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG1360.1744327728397912No Hit
GTCGGTGGGGGAGAAGGTACCATGAGGTGCCTTTTGTTTTAGGATGAAATATTCCATA1300.16673720933215336No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG1280.16417202149627408No Hit
GTCGGTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA1270.1628894275783344No Hit
GTCGGTGGGATCAAGAAAATTCCTCAAATGGAAAGTCTGAGTGGTCGGATGCCTCCCA1260.1616068336603948No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1210.15519386407069657No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1190.1526286762348173No Hit
GTCGGTGGGGGGCTGGAGGAATGGCTCAGTGGTTAAGAGCACTGACTGCTTGTCCAGA1160.14878089448099838No Hit
GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1160.14878089448099838No Hit
GTCGGTGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC1160.14878089448099838No Hit
GTCGGTGGGATGGACAGCCATAGAACGTTATGAAATGTTTAAATTTAAAATGTTTTAA1130.14493311272717946No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC1120.1436505188092398No Hit
GTCGGTGGGGAGCACTCAGGCCCTGCAGCAGGCCCTCCAAAGACTGAGGAAAGTGGAG1120.1436505188092398No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT1030.13210717354778304No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCGGA1030.13210717354778304No Hit
GTCGGTGGGGGGGCTGGAGATATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTGCGA1030.13210717354778304No Hit
GTCGGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGG1030.13210717354778304No Hit
GTCGGTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCTCCC1030.13210717354778304No Hit
GTCGGTGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC1030.13210717354778304No Hit
GTCGGTGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAG1010.12954198571190376No Hit
GTCGGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1010.12954198571190376No Hit
GTCGGTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA990.12697679787602448No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAA980.12569420395808484No Hit
GTCGGTGGGGCTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAGGGTCTGGGATT960.12312901612220556No Hit
GTCGGTGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT950.1218464222042659No Hit
GTCGGTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG930.11928123436838663No Hit
GTCGGTGGGGGAAAAATTTTTCACAATAATTTTTCCACCATAAATTGGATATAGTGCA930.11928123436838663No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC920.11799864045044699No Hit
GTCGGTGGGGCTTACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCT900.11543345261456771No Hit
GTCGGTGGGGCTGTCGGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAA890.11415085869662805No Hit
GTCGGTGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA880.11286826477868843No Hit
GTCGGTGGGGCTTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACGACTCT870.11158567086074878No Hit
GTCGGTGGGGGGGCTGGAGGAATGGCTCAGTGGTTAAGAGCACTGACTGCTTGTCCAG860.11030307694280914No Hit
GTCGGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA860.11030307694280914No Hit
GTCGGTGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC850.10902048302486948No Hit
GTCGGTGGGATAAATAAAATTTAGGAACAAAACCGGTGGTTTATCCTTGCATCTCTGC840.10773788910692986No Hit
GTCGGTGGGAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGTGATGG840.10773788910692986No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG830.1064552951889902No Hit
GTCGGTGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC830.1064552951889902No Hit
GTCGGTGGGGGGACTAGAGAGATGGCTCAGTGGTTAAGAGCATTGACTGCTCTTCCAA820.10517270127105058No Hit
GTCGGTGGGAGATCATGCTCTTTACACAGAGTACCGCTTATTTAATAAGACGGGATGT820.10517270127105058No Hit
GTCGGTGGGAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGGGATGG820.10517270127105058No Hit
GTCGGTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT820.10517270127105058No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAA810.10389010735311092No Hit
GTCGGTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAACACCAACTGCTTTTCCGAA810.10389010735311092No Hit
GTCGGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA810.10389010735311092No Hit
GTCGGTGGGGCTTTTCCTCTTCCGGGGACGGTGCTCAGAGGCACTCAGGATGGTCCAG800.10260751343517129No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTTAGTGGGTAAAAGCACCCGACTGCTCTTCCG800.10260751343517129No Hit
GTCGGTGGGGCTTTTTTCCCCCTTAGCGCGGCCGACTCTGCAGGTCTGCGTGGAGCCG800.10260751343517129No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCGA800.10260751343517129No Hit
GTCGGTGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA790.10132491951723166No Hit
GTCGGTGGGATCACGTTCTCCTGTGGGCCAGTATTGTGGAACGAGTCTGAGTTGTTTA790.10132491951723166No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGCC251.627815E-552.070125
AGCAGAT251.627815E-552.070127
TGTGAGA150.00715675652.07009525
TAGCGCG203.4049715E-452.07009524
GCAACGA203.4049715E-452.07009524
GTATATA150.00715675652.07009525
GTGAGAG307.825256E-752.07009527
GTGAGAA203.4049715E-452.07009526
CGGGCGC150.00715675652.07009526
CTGGTGC203.4049715E-452.07009526
TCTGCAT150.00715675652.07009526
CTGCATA150.00715675652.07009527
TCCTGGT203.4049715E-452.07009524
CCTGGTG150.00715675652.07009525
GTATGTC251.6340298E-552.03663635
CGTCACG251.6340298E-552.03663637
GTCATAG251.6340298E-552.03663639
GAGTGGT251.6340298E-552.03663639
GCAGGTC251.6340298E-552.03663640
TGTCATA251.6340298E-552.03663638
Produced by FastQC (version 0.11.5)