##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212551_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 77967 Sequences flagged as poor quality 0 Sequence length 58 %GC 57 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.764849231084945 37.0 34.0 38.0 32.0 38.0 2 34.520656175048416 36.0 33.0 38.0 28.0 38.0 3 35.34782664460605 37.0 33.0 38.0 30.0 38.0 4 35.16605743455565 37.0 33.0 38.0 30.0 38.0 5 34.87989790552413 37.0 33.0 38.0 28.0 38.0 6 32.05548501289007 34.0 29.0 36.0 23.0 38.0 7 36.760257544858725 38.0 36.0 38.0 33.0 39.0 8 36.66575602498493 38.0 36.0 38.0 33.0 39.0 9 34.65986891890159 35.0 32.0 38.0 30.0 39.0 10 33.66380648222966 35.0 31.0 38.0 27.0 39.0 11 34.343658214372745 36.0 32.0 38.0 27.0 39.0 12 34.796144522682674 36.0 33.0 38.0 28.0 39.0 13 34.6812112816961 36.0 33.0 38.0 28.0 39.0 14 34.97230879731168 37.0 33.0 38.0 29.0 39.0 15 35.06929854938628 37.0 33.0 38.0 29.0 39.0 16 35.390524196134265 38.0 35.0 38.0 30.0 39.0 17 34.958880038990856 37.0 33.0 38.0 29.0 39.0 18 34.802685751664164 36.0 33.0 38.0 29.0 39.0 19 34.57040799312529 36.0 33.0 38.0 28.0 39.0 20 34.41003244962612 36.0 33.0 38.0 27.0 39.0 21 34.13628842972027 36.0 33.0 38.0 27.0 39.0 22 33.87951312734875 36.0 33.0 38.0 27.0 39.0 23 33.658637628740365 36.0 32.0 38.0 26.0 39.0 24 33.522618543742865 36.0 32.0 38.0 26.0 39.0 25 33.475496043197765 36.0 32.0 38.0 26.0 39.0 26 32.88297613092719 35.0 31.0 38.0 24.0 39.0 27 32.452717175215156 35.0 31.0 38.0 23.0 38.0 28 32.111483063347315 35.0 30.0 38.0 22.0 38.0 29 32.081649928816034 35.0 30.0 38.0 23.0 38.0 30 31.666923185450255 35.0 30.0 38.0 20.0 38.0 31 30.793925635204637 34.0 28.0 38.0 18.0 38.0 32 30.725088819628816 34.0 28.0 38.0 17.0 38.0 33 30.583605884540894 34.0 28.0 37.0 18.0 38.0 34 29.54247309759257 33.0 27.0 36.0 13.0 38.0 35 29.12112816961022 33.0 26.0 36.0 11.0 38.0 36 30.42332012261598 35.0 28.0 38.0 13.0 39.0 37 30.37995562225044 34.0 28.0 38.0 14.0 39.0 38 30.005476676029602 34.0 28.0 37.0 12.0 38.0 39 30.077648235792065 34.0 28.0 38.0 12.0 38.0 40 29.82127053753511 34.0 28.0 37.0 9.0 38.0 41 29.964754319135018 35.0 28.0 38.0 2.0 39.0 42 29.72419100388626 35.0 28.0 38.0 2.0 39.0 43 29.664127130709147 35.0 27.0 38.0 2.0 39.0 44 29.588967127117883 34.0 28.0 38.0 2.0 39.0 45 29.093744789462207 34.0 27.0 38.0 2.0 39.0 46 28.48893762745777 34.0 26.0 37.0 2.0 39.0 47 28.39693716572396 33.0 26.0 37.0 2.0 39.0 48 28.36016519809663 33.0 26.0 37.0 2.0 39.0 49 28.05801172290841 33.0 25.0 37.0 2.0 39.0 50 27.85254017725448 33.0 25.0 36.0 2.0 39.0 51 27.59736811728039 33.0 24.0 37.0 2.0 39.0 52 27.2911488193723 33.0 23.0 36.0 2.0 39.0 53 26.932099477984277 33.0 23.0 36.0 2.0 38.0 54 26.780625136275603 33.0 23.0 36.0 2.0 39.0 55 26.6153500840099 33.0 22.0 36.0 2.0 39.0 56 26.304359536727077 33.0 18.0 37.0 2.0 39.0 57 25.808072646119513 33.0 15.0 36.0 2.0 39.0 58 25.177280131337618 32.0 2.0 36.0 2.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -2.835638038450881 1 2 -3.8598175203742535 1 3 -3.9067998783822055 1 4 -3.8399258616578926 1 5 -5.959772463979377 1 6 -5.194086034975683 1 7 -3.532853331794513 1 8 -3.370976559447925 1 9 -4.141761310756088 1 10 -5.245889272197367 1 11 -1.0670940528319974 1 12 -2.1906307294923124 1 13 -1.5611315818314822 1 14 -2.637991729008604 1 15 -1.7982003589139666 1 16 -1.845709866973806 1 17 -0.6539955947157878 1 18 -1.4039279695044513 1 19 -1.425035944140106 1 20 -0.7198662463723835 1 21 -2.711265592981764 1 22 -2.976923251267465 1 23 -1.3196302824824784 1 24 -1.5759620986048475 1 25 -2.608416489333358 1 26 -1.6962858675332022 1 27 -0.8724159093190416 1 28 -4.1362397475718105 1 29 -2.752793607162463 1 30 -3.1921671257047777 1 31 -1.9267159444557755 1 32 -2.656864404564349 1 33 -0.42982358025127354 1 34 -1.9502814540012672 1 35 -2.414833112077634 1 36 -2.148175964172591 1 37 -1.8498303232968567 1 38 -0.9084132234105518 1 39 -2.632486917506384 1 40 -1.4608957418640003 1 41 -0.33118944725287136 1 42 -0.26939218406788257 1 43 -0.15408800579593773 1 44 -0.3899920756219224 1 45 -0.21277300266994104 1 46 0.5006867752157653 1 47 -0.18806660209190795 1 48 -0.6033441153632886 1 49 -2.5866876399526575 1 50 0.008835057848525452 1 51 -0.6860893271391149 1 52 -0.13369028868942934 1 53 0.41667235686136195 1 54 -0.9686311540608301 1 55 0.9991302774416084 1 56 0.8546109419192014 1 57 1.3122187312028153 1 58 -0.015310634878449747 2 1 -1.017394795207636 2 2 -1.4152229257796591 2 3 -1.9459890675713964 2 4 -1.8980339697660042 2 5 -3.6503130045199157 2 6 -1.5636806295702748 2 7 -1.3159614399026154 2 8 -2.181111694583059 2 9 -2.739058608053387 2 10 -2.512105488413585 2 11 -1.0542562149941617 2 12 -0.35617127003285276 2 13 -0.5401856358855355 2 14 -0.5042079452248203 2 15 -1.1833354940491034 2 16 -0.778142299406241 2 17 -2.024265864986063 2 18 -1.728252293828774 2 19 -1.0270629711671333 2 20 -1.9624338139399455 2 21 -1.4315358632520372 2 22 -1.8357070350512465 2 23 -2.831792444644641 2 24 -1.415826963469712 2 25 -0.8901732460901144 2 26 -1.7293939756413117 2 27 -0.42984834175147313 2 28 -0.9240775854096483 2 29 -0.2399557693246237 2 30 0.6355355769979241 2 31 0.32260837986854796 2 32 0.7782707305707888 2 33 -1.681850607278303 2 34 0.9362050324852191 2 35 0.025707428462904858 2 36 0.3856078196111916 2 37 1.040710217243685 2 38 1.360505695508369 2 39 0.6391347041152358 2 40 2.195861014892756 2 41 0.9201619040984781 2 42 1.096148356472657 2 43 2.111452534744604 2 44 1.6877106270807793 2 45 -0.05669192158886105 2 46 2.4114975860265773 2 47 2.0186901546648492 2 48 2.777736965717793 2 49 2.4768258735608555 2 50 1.5480242470377163 2 51 0.826072835023048 2 52 1.8832016032024619 2 53 3.0403210055100125 2 54 1.7205580351283594 2 55 1.9214275747389067 2 56 1.5775839148921733 2 57 -0.16075424177015663 2 58 0.9657704462026295 3 1 -0.4216630878905647 3 2 -0.2896131696820987 3 3 -0.38867199440066 3 4 -1.0212047014733159 3 5 -2.0125081264711397 3 6 -0.39843672713125144 3 7 0.04196538936567151 3 8 0.15242489078278965 3 9 -1.365887876346072 3 10 -0.3645445128038318 3 11 0.4780608581765762 3 12 0.8541945836256843 3 13 1.1896924128949493 3 14 1.097011566970302 3 15 0.6032498718045574 3 16 0.2340528225449816 3 17 -0.04621708449825945 3 18 1.3205281939761022 3 19 -0.11059955653298204 3 20 0.5174442348405393 3 21 -0.493730985203257 3 22 -0.6631460594414946 3 23 0.1279636529163355 3 24 -0.026192817128247725 3 25 0.6348267539098842 3 26 0.3175572438708869 3 27 0.7927126338582795 3 28 -0.12041904882428156 3 29 0.5240686209192766 3 30 -0.7821473498313445 3 31 2.0317547213319607 3 32 1.8197341452049365 3 33 1.0309542707704793 3 34 1.5362050324852206 3 35 1.369609867487295 3 36 2.269754161074605 3 37 1.398027290414415 3 38 0.5227008174595866 3 39 3.1598664114323114 3 40 1.930007356356171 3 41 1.8982106845862852 3 42 2.856514210131195 3 43 2.093769607915334 3 44 2.8980764807393165 3 45 2.551234907679433 3 46 1.3114975860265758 3 47 3.6662511302746026 3 48 2.965541843766573 3 49 3.048777093073049 3 50 2.271194978745033 3 51 2.232170395998658 3 52 3.367957700763437 3 53 1.6994673469734245 3 54 2.025436083908847 3 55 2.7598422088852494 3 56 0.8031936709897352 3 57 1.0465628314005748 3 58 -0.2269124806266376 4 1 -1.842394795207639 4 2 -2.0902229257796634 4 3 -2.2459890675713936 4 4 -1.9980339697659986 4 5 -3.050313004519918 4 6 -1.9136806295702762 4 7 -0.8159614399026154 4 8 -1.1561116945830605 4 9 -1.789058608053388 4 10 -2.1621054884135837 4 11 -0.9792562149941588 4 12 -0.9811712700328528 4 13 -0.5401856358855355 4 14 -1.3792079452248203 4 15 -0.6583354940491049 4 16 -0.6031422994062439 4 17 -0.19926586498606014 4 18 -0.8532522938287741 4 19 0.047937028832869544 4 20 -0.43743381393994696 4 21 -0.4065358632520315 4 22 -0.6107070350512487 4 23 0.04320755535535881 4 24 -0.26582696346971346 4 25 -1.1901732460901187 4 26 0.9206060243586904 4 27 1.1451516582485297 4 28 0.7509224145903524 4 29 0.010044230675376298 4 30 2.2355355769979255 4 31 -0.6273916201314513 4 32 1.3282707305707895 4 33 0.5181493927216998 4 34 0.43620503248521914 4 35 0.8507074284629077 4 36 -0.5643921803888077 4 37 0.4657102172436822 4 38 -0.06449430449163174 4 39 0.6141347041152372 4 40 0.4208610148927576 4 41 2.3451619040984824 4 42 1.0461483564726564 4 43 2.3864525347446026 4 44 2.3627106270807765 4 45 2.0183080784111382 4 46 -0.11350241397342486 4 47 0.09369015466484854 4 48 0.9277369657177914 4 49 0.45182587356085335 4 50 0.3480242470377135 4 51 2.426072835023046 4 52 0.6582016032024605 4 53 2.4653210055100097 4 54 2.3455580351283594 4 55 2.6964275747389053 4 56 2.877583914892174 4 57 1.8142457582298448 4 58 3.065770446202631 5 1 -1.147950350763196 5 2 -1.865222925779662 5 3 -1.2515446231269536 5 4 -1.72581174754378 5 5 -2.1003130045199185 5 6 -2.322013962903611 5 7 -0.7076281065692882 5 8 -0.7061116945830648 5 9 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4.420246469259936 5 51 4.142739501689714 5 52 3.291534936535797 5 53 3.9986543388433446 5 54 3.5094469240172472 5 55 3.163094241405574 5 56 3.494250581558841 5 57 2.450356869340954 5 58 2.4879926684248517 6 1 -1.4948338195978863 6 2 -0.8505887794381977 6 3 -1.5837939456201724 6 4 -1.8504729941562488 6 5 -2.841776419154062 6 6 -1.9594123368873504 6 7 0.017575145463233355 6 8 -0.7500141336074506 6 9 -1.0000342178094854 6 10 -0.4864957323160226 6 11 -0.3512074345063567 6 12 -0.6580005383255383 6 13 -0.029819782227001213 6 14 -0.610305506200433 6 15 -1.5187013477076405 6 16 -0.7659471774550184 6 17 -0.8754853771811852 6 18 -0.7526425377312123 6 19 -2.5008434589720068 6 20 0.200371064108829 6 21 -2.4205602534959354 6 22 -2.22412166919759 6 23 -2.7988656153763465 6 24 -2.1725342805428802 6 25 -1.096880563163289 6 26 -1.1946378780803357 6 27 -1.1828971222392788 6 28 -1.0228580732145254 6 29 -0.8173947937148682 6 30 -0.8797083254411007 6 31 0.9341937457222045 6 32 0.9172951208146891 6 33 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0.8131345991092935 118 48 -0.4778185898377636 118 49 -0.9176185708835902 118 50 -0.20197575296228365 118 51 -0.9461493871991742 118 52 -0.10846506346420526 118 53 -0.09023455004554393 118 54 -1.35721974264942 118 55 -0.17023909192775832 118 56 -0.41686052955227026 118 57 -0.6607542417701566 118 58 -0.6675628871307033 119 1 -1.177760648866176 119 2 -0.6554668282186853 119 3 -1.0715988236689569 119 4 -1.1431559209855138 119 5 -2.329581297202843 119 6 -1.105753800301983 119 7 -1.177546805756279 119 8 -0.21342876775379693 119 9 -0.7561317787850967 119 10 -0.681617683535535 119 11 0.6244023215912051 119 12 -0.1214151724718775 119 13 0.3116436324071472 119 14 0.5116457133117649 119 15 -0.39675012819544264 119 16 -0.44887400672331523 119 17 -0.8023146454738637 119 18 -0.9965449767556009 119 19 -0.9642580931183531 119 20 0.17598082020639083 119 21 -0.44495049739837356 119 22 -0.004609474075635944 119 23 0.4694270675504839 119 24 -0.3920464756648343 119 25 -0.8773683680413384 119 26 1.488288951187954 119 27 1.5488101948338908 119 28 -0.6326141707755042 119 29 0.719190572138789 119 30 0.3154136257784117 119 31 1.4219986237709854 119 32 1.6733926817903004 119 33 0.5919298805265747 119 34 0.48742454468034424 119 35 1.589122062609249 119 36 0.903900502538022 119 37 0.37363704651197693 119 38 1.156847158923 119 39 1.0379151919201135 119 40 0.7592756490391004 119 41 2.5567472699521403 119 42 3.441880063789732 119 43 2.557184242061677 119 44 2.7273447734222422 119 45 3.18538124914285 119 46 3.360278073831452 119 47 2.7638121058843623 119 48 2.867980868156817 119 49 2.439020995512074 119 50 4.149243759232839 119 51 2.329731371608414 119 52 2.0264942861292923 119 53 3.553125883558792 119 54 4.049826327811285 119 55 2.491549525958419 119 56 2.3641692807458305 119 57 1.9977823435956985 119 58 3.3340631291294613 120 1 -1.651218324619407 120 2 -1.5005170434267185 120 3 -2.478342008747866 120 4 -2.9774457344718854 120 5 -3.853254180990504 120 6 -1.6416218060408632 120 7 -0.6997849693143863 120 8 -2.506111694583062 120 9 -2.3802350786416184 120 10 -2.8150466648841714 120 11 -0.7880797444059269 120 12 -0.7605830347387368 120 13 -0.6740091652973064 120 14 -1.0012667687542347 120 15 -0.7274531411079295 120 16 0.368916524123172 120 17 -0.27720704145664854 120 18 -0.16942876441700605 120 19 0.9008782053034565 120 20 0.7419779507659356 120 21 1.8714053132185526 120 22 0.38635178847816576 120 23 1.9593840259435922 120 24 1.0003495071185213 120 25 1.8995326362628262 120 26 1.019135436123392 120 27 1.224563422954411 120 28 1.231804767531525 120 29 0.6144559953812596 120 30 1.010535576997924 120 31 1.5152554386920762 120 32 2.007682495276672 120 33 1.7490317456628723 120 34 1.742087385426398 120 35 3.8315897814040802 120 36 1.9591372313758946 120 37 -0.07399566510925837 120 38 0.15756451903778057 120 39 0.3714876452917082 120 40 0.7664492501868736 120 41 0.431926609980831 120 42 1.6240895329432448 120 43 -0.2606062887848104 120 44 -0.010818784683927163 120 45 0.42713160782290416 120 46 -0.3355612375028372 120 47 2.1275136840766145 120 48 0.9483252010119081 120 49 1.6327082265020323 120 50 0.9039065999788924 120 51 0.917249305611282 120 52 1.7287898384965779 120 53 2.035909240804127 120 54 2.044087446893066 120 55 3.119956986503613 120 56 2.033466267833351 120 57 2.8686575229357274 120 58 1.2422410344379244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 85.0 3 26.0 4 35.0 5 39.0 6 68.0 7 86.0 8 66.0 9 126.0 10 178.0 11 233.0 12 310.0 13 422.0 14 472.0 15 572.0 16 663.0 17 653.0 18 707.0 19 726.0 20 751.0 21 887.0 22 1210.0 23 1214.0 24 1186.0 25 1443.0 26 1788.0 27 2149.0 28 2594.0 29 3197.0 30 3911.0 31 4740.0 32 5576.0 33 6435.0 34 7632.0 35 8566.0 36 8827.0 37 7754.0 38 2395.0 39 245.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 65.35970346428617 20.16494157784704 3.451460233175575 11.023894724691216 11 35.758718432157195 17.996075262611104 16.79300216758372 29.452204137647982 12 35.967781240781356 11.494606692575065 31.728808341990845 20.808803724652737 13 22.746803134659537 14.90245873254069 33.54880911154719 28.801929021252583 14 17.22395372401144 17.23293188143702 44.55089973963344 20.992214654918108 15 23.928072133081947 13.587799966652558 40.252927520617696 22.2312003796478 16 29.516333833544962 13.741711236805315 34.24269242115254 22.499262508497182 17 30.819449254171637 14.93452358048918 23.37783934228584 30.868187823053344 18 31.395617366314276 15.930668172020937 25.772349379041366 26.901365082623425 19 30.600020522292343 20.868606023292802 24.998717356728747 23.53265609768611 20 31.011838341862585 20.397091076994112 26.883168520014877 21.707902061128426 21 33.06014082881219 20.093116318442416 24.038375210024753 22.80836764272064 22 33.3128118306463 18.5847858709454 26.240588967127117 21.861813331281184 23 37.34272192081265 20.888324547564995 23.309861864635035 18.459091666987316 24 39.30710721752626 19.964598591639625 20.590536536562215 20.137757654271898 25 32.72794900406582 19.848140880115945 18.142290969256226 29.281619146562004 26 28.685948823189893 18.23638812287565 22.402360033348298 30.67530302058616 27 26.84719883815081 18.369812484737878 23.14316192630483 31.63982675080648 28 28.397532099383028 24.183886815202474 20.30630699965367 27.11227408576083 29 32.298535033481286 20.1118608410088 22.824999358596095 24.76460476691382 30 27.21555541295001 19.093884361162804 28.2859526406982 25.404607585188987 31 35.039763981528985 16.798358132375576 26.466136480246284 21.69574140584915 32 41.233753736800914 15.674676999268677 25.12926444361761 17.9623048203128 33 41.7820257874142 15.033677593174675 24.08749759445763 19.09679902495349 34 31.641071039092672 18.48402037386295 31.863028110285725 18.011880476758655 35 28.79890167699552 27.85839845003015 29.749669604937324 13.593030268037005 36 29.687139080666274 32.28832482098401 23.22203115776506 14.802504940584656 37 32.44731370485407 26.81674666940473 22.53253587288549 18.203403752855714 38 29.738541118484385 27.227919752531797 23.361870900666162 19.67166822831765 39 33.2644389385984 25.048461429836838 22.031657188338446 19.655442443226313 40 31.473638277118948 21.586067046562967 23.0568817701114 23.883412906206683 41 28.490917260414662 23.159381218306695 25.815520893510495 22.534180627768148 42 26.101412066752243 23.762516046213094 25.263157894736842 24.872913992297818 43 28.43610412281152 17.63875340144786 25.573753658160907 28.35138881757971 44 26.18072583026304 15.659139633105253 25.188389796782936 32.97174473984877 45 29.817467652495377 18.96436639967139 25.0 26.21816594783323 46 28.942605168230656 23.23136368887919 24.992618647222685 22.833412495667467 47 27.802460258352806 21.50928375150877 26.587739798145815 24.100516191992604 48 30.494459638175215 18.349318850069977 28.812449443395867 22.34377206835895 49 27.05781133626114 19.591904871972673 27.12458586948147 26.22569792228472 50 21.723216808363084 17.700922096935763 30.962165771967225 29.61369532273393 51 21.07581283065386 17.60619446299245 33.93471672915918 27.383275977194515 52 20.600395470070108 18.53701753935441 35.03890501014355 25.823681980431935 53 19.06009244992296 20.426296866974834 36.66153055983564 23.852080123266564 54 20.79555087466927 19.22602686942896 36.11960235300162 23.85881990290015 55 21.834930750057815 18.186139733278516 29.353495901534032 30.625433615129634 56 21.635375303596902 20.879756351439916 24.99196833596772 32.49290000899546 57 25.64217884632683 20.697498104624714 26.080363912054587 27.57995913699387 58 24.835837006386615 26.415142831442196 24.76259011295442 23.98643004921677 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 28.5 12 57.0 13 66.5 14 76.0 15 68.5 16 72.5 17 84.0 18 125.5 19 167.0 20 194.0 21 221.0 22 266.5 23 324.0 24 336.0 25 451.5 26 567.0 27 697.5 28 879.5 29 931.0 30 1004.5 31 1078.0 32 1118.0 33 1158.0 34 1299.5 35 1351.0 36 1261.0 37 1030.5 38 800.0 39 842.5 40 885.0 41 977.5 42 925.0 43 780.0 44 999.5 45 1219.0 46 1219.5 47 1577.5 48 1935.0 49 1694.0 50 1453.0 51 1728.5 52 2004.0 53 2050.5 54 3083.5 55 4070.0 56 3656.5 57 3243.0 58 3401.0 59 4361.5 60 5164.0 61 6231.0 62 7298.0 63 7689.5 64 8081.0 65 8081.0 66 6693.5 67 5306.0 68 4854.0 69 4402.0 70 3486.0 71 2570.0 72 1990.5 73 1182.0 74 953.0 75 806.0 76 659.0 77 542.0 78 360.0 79 295.0 80 247.0 81 199.0 82 151.0 83 103.0 84 77.0 85 36.5 86 22.0 87 19.0 88 16.0 89 15.5 90 15.0 91 8.5 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.029499660112611747 19 0.0038477817538189237 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005130375671758565 25 0.0 26 0.0025651878358792825 27 0.20521502687034257 28 0.0076955635076378474 29 0.016673720933215334 30 0.06669488373286134 31 0.008978157425577487 32 0.03334744186643067 33 0.028217066194672107 34 0.03078225403055139 35 0.038477817538189234 36 0.05258635063552529 37 0.06797747765080098 38 0.07439044724049919 39 0.08978157425577488 40 0.0654122898149217 41 0.09234676209165416 42 0.08593379250195596 43 0.07567304115843883 44 0.08978157425577488 45 0.0808034168301974 46 0.0872163864198956 47 0.11415085869662805 48 0.10773788910692986 49 0.11928123436838663 50 0.12954198571190376 51 0.11671604653250733 52 0.10902048302486948 53 0.11158567086074878 54 0.1398027370554209 55 0.17058499108597228 56 0.19238908769094618 57 0.1872587120191876 58 0.18982389985506687 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 77967.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.096053458514504 #Duplication Level Percentage of deduplicated Percentage of total 1 71.82607347698031 23.77159567509331 2 10.727019066811346 7.100439929713853 3 5.041853976127732 5.005964061718419 4 2.8367694931018446 3.755434991727269 5 1.9531855526274997 3.2321366732078958 6 1.2982483335916912 2.5780137750586785 7 0.9649666718338242 2.2355611989687945 8 0.7866997364749652 2.082932522733977 9 0.5386761742365525 1.604524991342491 >10 3.4413269260579757 20.391960701322354 >50 0.352658502557743 8.143188784998781 >100 0.2015191443187103 12.18977259609835 >500 0.023252208959851185 4.891813203021791 >1k 0.0077507363199503946 3.016660894994036 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 1282 1.64428540279862 No Hit GTCGGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 1070 1.372375492195416 No Hit GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 881 1.1299652417048238 No Hit GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 665 0.8529249554298614 No Hit GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 655 0.8400990162504649 No Hit GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 574 0.736208908897354 No Hit GTCGGTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC 521 0.668231431246553 No Hit GTCGGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 518 0.6643836494927341 No Hit GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 402 0.5156027550117358 No Hit GTCGGTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 396 0.5079071915040979 No Hit GTCGGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 377 0.4835379070632447 No Hit GTCGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 364 0.46686418613002933 No Hit GTCGGTGGGATTGCAGATATCTGATGGATGTAGTTTTCTTTTTTTAAATTAGAAATGC 323 0.4142778354945041 No Hit GTCGGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 288 0.3693870483666166 No Hit GTCGGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 283 0.36297407877691845 No Hit GTCGGTGGGAGTCTTAACATACATGTAGCAGATTGTATGTCATAGGGTACTTCTGTTT 279 0.35784370310515984 No Hit GTCGGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 266 0.34116998217194455 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 264 0.3386047943360653 No Hit GTCGGTGGGGAGCCAGCCCCAGAGAGGTGAGAGGAAGTGGTGTCAAGGGCTTTGCCCT 252 0.3232136673207896 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA 248 0.318083291649031 No Hit GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT 225 0.28858363153641925 No Hit GTCGGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT 221 0.2834532558646607 No Hit GTCGGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT 220 0.282170661946721 No Hit GTCGGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 218 0.2796054741108418 No Hit GTCGGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 217 0.27832288019290213 No Hit GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT 215 0.27575769235702285 No Hit GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA 209 0.268062128849385 No Hit GTCGGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT 183 0.2347146869829543 No Hit GTCGGTGGGATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAG 164 0.21034540254210116 No Hit GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 163 0.20906280862416152 No Hit GTCGGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 159 0.20393243295240296 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGCTCTTCCGA 156 0.200084651198584 No Hit GTCGGTGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA 154 0.19751946336270476 No Hit GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC 153 0.19623686944476507 No Hit GTCGGTGGGGAATACCAATTTTAAAGTTTCTGAAGAATTGCTAAGAGTCAGAGGAATG 148 0.18982389985506687 No Hit GTCGGTGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 148 0.18982389985506687 No Hit GTCGGTGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG 141 0.1808457424294894 No Hit GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC 138 0.17699796067567047 No Hit GTCGGTGGGAATTTGGAAGGGTGGAAATGATGTAAATATAGTATTCATGTATGAAATT 138 0.17699796067567047 No Hit GTCGGTGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG 136 0.1744327728397912 No Hit GTCGGTGGGGGAGAAGGTACCATGAGGTGCCTTTTGTTTTAGGATGAAATATTCCATA 130 0.16673720933215336 No Hit GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGGAAGAGCACCCGACTGCTCTTCCG 128 0.16417202149627408 No Hit GTCGGTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA 127 0.1628894275783344 No Hit GTCGGTGGGATCAAGAAAATTCCTCAAATGGAAAGTCTGAGTGGTCGGATGCCTCCCA 126 0.1616068336603948 No Hit GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC 121 0.15519386407069657 No Hit GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC 119 0.1526286762348173 No Hit GTCGGTGGGGGGCTGGAGGAATGGCTCAGTGGTTAAGAGCACTGACTGCTTGTCCAGA 116 0.14878089448099838 No Hit GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC 116 0.14878089448099838 No Hit GTCGGTGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC 116 0.14878089448099838 No Hit GTCGGTGGGATGGACAGCCATAGAACGTTATGAAATGTTTAAATTTAAAATGTTTTAA 113 0.14493311272717946 No Hit GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC 112 0.1436505188092398 No Hit GTCGGTGGGGAGCACTCAGGCCCTGCAGCAGGCCCTCCAAAGACTGAGGAAAGTGGAG 112 0.1436505188092398 No Hit GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT 103 0.13210717354778304 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCGGA 103 0.13210717354778304 No Hit GTCGGTGGGGGGGCTGGAGATATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTGCGA 103 0.13210717354778304 No Hit GTCGGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGG 103 0.13210717354778304 No Hit GTCGGTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCTCCC 103 0.13210717354778304 No Hit GTCGGTGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC 103 0.13210717354778304 No Hit GTCGGTGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAG 101 0.12954198571190376 No Hit GTCGGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA 101 0.12954198571190376 No Hit GTCGGTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA 99 0.12697679787602448 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAA 98 0.12569420395808484 No Hit GTCGGTGGGGCTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAGGGTCTGGGATT 96 0.12312901612220556 No Hit GTCGGTGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT 95 0.1218464222042659 No Hit GTCGGTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG 93 0.11928123436838663 No Hit GTCGGTGGGGGAAAAATTTTTCACAATAATTTTTCCACCATAAATTGGATATAGTGCA 93 0.11928123436838663 No Hit GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC 92 0.11799864045044699 No Hit GTCGGTGGGGCTTACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCT 90 0.11543345261456771 No Hit GTCGGTGGGGCTGTCGGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAA 89 0.11415085869662805 No Hit GTCGGTGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA 88 0.11286826477868843 No Hit GTCGGTGGGGCTTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACGACTCT 87 0.11158567086074878 No Hit GTCGGTGGGGGGGCTGGAGGAATGGCTCAGTGGTTAAGAGCACTGACTGCTTGTCCAG 86 0.11030307694280914 No Hit GTCGGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA 86 0.11030307694280914 No Hit GTCGGTGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC 85 0.10902048302486948 No Hit GTCGGTGGGATAAATAAAATTTAGGAACAAAACCGGTGGTTTATCCTTGCATCTCTGC 84 0.10773788910692986 No Hit GTCGGTGGGAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGTGATGG 84 0.10773788910692986 No Hit GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG 83 0.1064552951889902 No Hit GTCGGTGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 83 0.1064552951889902 No Hit GTCGGTGGGGGGACTAGAGAGATGGCTCAGTGGTTAAGAGCATTGACTGCTCTTCCAA 82 0.10517270127105058 No Hit GTCGGTGGGAGATCATGCTCTTTACACAGAGTACCGCTTATTTAATAAGACGGGATGT 82 0.10517270127105058 No Hit GTCGGTGGGAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGGGATGG 82 0.10517270127105058 No Hit GTCGGTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGT 82 0.10517270127105058 No Hit GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAA 81 0.10389010735311092 No Hit GTCGGTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAACACCAACTGCTTTTCCGAA 81 0.10389010735311092 No Hit GTCGGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA 81 0.10389010735311092 No Hit GTCGGTGGGGCTTTTCCTCTTCCGGGGACGGTGCTCAGAGGCACTCAGGATGGTCCAG 80 0.10260751343517129 No Hit GTCGGTGGGGGGGCTGGTGAGATGGCTTAGTGGGTAAAAGCACCCGACTGCTCTTCCG 80 0.10260751343517129 No Hit GTCGGTGGGGCTTTTTTCCCCCTTAGCGCGGCCGACTCTGCAGGTCTGCGTGGAGCCG 80 0.10260751343517129 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCGA 80 0.10260751343517129 No Hit GTCGGTGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA 79 0.10132491951723166 No Hit GTCGGTGGGATCACGTTCTCCTGTGGGCCAGTATTGTGGAACGAGTCTGAGTTGTTTA 79 0.10132491951723166 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.001282593917939641 0.0 0.0 0.0 0.0 11 0.008978157425577489 0.0 0.0 0.0 0.0 12 0.008978157425577489 0.0 0.0 0.0 0.0 13 0.008978157425577489 0.0 0.0 0.0 0.0 14 0.016673720933215334 0.0 0.0 0.0 0.0 15 0.016673720933215334 0.0 0.0 0.0 0.0 16 0.017956314851154977 0.0 0.0 0.0 0.0 17 0.019238908769094617 0.0 0.0 0.0 0.0 18 0.019238908769094617 0.0 0.0 0.0 0.0 19 0.019238908769094617 0.0 0.0 0.0 0.0 20 0.02308669052291354 0.0 0.0 0.0 0.0 21 0.024369284440853182 0.0 0.0 0.0 0.0 22 0.024369284440853182 0.0 0.0 0.0 0.0 23 0.02565187835879282 0.0 0.0 0.0 0.0 24 0.028217066194672104 0.0 0.0 0.0 0.0 25 0.029499660112611747 0.0 0.0 0.0 0.0 26 0.030782254030551386 0.0 0.0 0.0 0.0 27 0.03206484794849103 0.0 0.0 0.0 0.0 28 0.04617338104582708 0.0 0.0 0.0 0.0 29 0.048738568881706364 0.0 0.0 0.0 0.0 30 0.05515153847140457 0.0 0.0 0.0 0.0 31 0.05515153847140457 0.0 0.0 0.0 0.0 32 0.05515153847140457 0.0 0.0 0.0 0.0 33 0.05643413238934421 0.0 0.0 0.0 0.0 34 0.057716726307283854 0.0 0.0 0.0 0.0 35 0.06028191414316313 0.0 0.0 0.0 0.0 36 0.06284710197904242 0.0 0.0 0.0 0.0 37 0.06284710197904242 0.0 0.0 0.0 0.0 38 0.0654122898149217 0.0 0.0 0.0 0.0 39 0.06669488373286134 0.0 0.0 0.0 0.0 40 0.07695563507637847 0.0 0.0 0.0 0.0 41 0.07695563507637847 0.0 0.0 0.0 0.0 42 0.07823822899431811 0.0 0.0 0.0 0.0 43 0.08080341683019739 0.0 0.0 0.0 0.0 44 0.08336860466607668 0.0 0.0 0.0 0.0 45 0.08465119858401632 0.0 0.0 0.0 0.0 46 0.0872163864198956 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTGCC 25 1.627815E-5 52.0701 25 AGCAGAT 25 1.627815E-5 52.0701 27 TGTGAGA 15 0.007156756 52.070095 25 TAGCGCG 20 3.4049715E-4 52.070095 24 GCAACGA 20 3.4049715E-4 52.070095 24 GTATATA 15 0.007156756 52.070095 25 GTGAGAG 30 7.825256E-7 52.070095 27 GTGAGAA 20 3.4049715E-4 52.070095 26 CGGGCGC 15 0.007156756 52.070095 26 CTGGTGC 20 3.4049715E-4 52.070095 26 TCTGCAT 15 0.007156756 52.070095 26 CTGCATA 15 0.007156756 52.070095 27 TCCTGGT 20 3.4049715E-4 52.070095 24 CCTGGTG 15 0.007156756 52.070095 25 GTATGTC 25 1.6340298E-5 52.036636 35 CGTCACG 25 1.6340298E-5 52.036636 37 GTCATAG 25 1.6340298E-5 52.036636 39 GAGTGGT 25 1.6340298E-5 52.036636 39 GCAGGTC 25 1.6340298E-5 52.036636 40 TGTCATA 25 1.6340298E-5 52.036636 38 >>END_MODULE