FastQCFastQC Report
Tue 31 May 2016
SRR212550_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212550_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80530
Sequences flagged as poor quality0
Sequence length58
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA12181.5124798211846515No Hit
GTCGGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG10151.2603998509872099No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG9051.1238047932447535No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT8451.0492983981125046No Hit
GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC7580.9412641251707439No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5980.7425804048180803No Hit
GTCGGTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC5920.7351297653048553No Hit
GTCGGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC4620.5736992425183162No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4200.521544765925742No Hit
GTCGGTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT3870.48056624860300506No Hit
GTCGGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3860.47932447535080097No Hit
GTCGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT3720.4619396498199429No Hit
GTCGGTGGGATTGCAGATATCTGATGGATGTAGTTTTCTTTTTTTAAATTAGAAATGC3080.3824661616788774No Hit
GTCGGTGGGAGTCTTAACATACATGTAGCAGATTGTATGTCATAGGGTACTTCTGTTT2860.35514715013038617No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA2530.31416863280764934No Hit
GTCGGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT2530.31416863280764934No Hit
GTCGGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2490.30920153979883275No Hit
GTCGGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC2460.3054762200422203No Hit
GTCGGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2410.29926735378119956No Hit
GTCGGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2350.2918167142679747No Hit
GTCGGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2320.28809139451136223No Hit
GTCGGTGGGGAGCCAGCCCCAGAGAGGTGAGAGGAAGTGGTGTCAAGGGCTTTGCCCT2300.28560784800695393No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT2190.2719483422327083No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA2150.2669812492238917No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA2120.26325592946727927No Hit
GTCGGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACT1860.23096982490997145No Hit
GTCGGTGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1810.22476095864895068No Hit
GTCGGTGGGATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGCAG1690.20985967962250093No Hit
GTCGGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1640.20365081336148017No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1630.20240904010927607No Hit
GTCGGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1590.19744194710045945No Hit
GTCGGTGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1550.19247485409164286No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGCTCTTCCGA1440.17881534831739723No Hit
GTCGGTGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG1410.17509002856078482No Hit
GTCGGTGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC1310.16267229603874334No Hit
GTCGGTGGGGAGCACTCAGGCCCTGCAGCAGGCCCTCCAAAGACTGAGGAAAGTGGAG1250.15522165652551845No Hit
GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1230.15273811002111015No Hit
GTCGGTGGGAATTTGGAAGGGTGGAAATGATGTAAATATAGTATTCATGTATGAAATT1220.151496336768906No Hit
GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1210.15025456351670186No Hit
GTCGGTGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAAG1200.1490127902644977No Hit
GTCGGTGGGGAATACCAATTTTAAAGTTTCTGAAGAATTGCTAAGAGTCAGAGGAATG1180.1465292437600894No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCT1180.1465292437600894No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCTC1170.14528747050788526No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAA1150.14280392400347697No Hit
GTCGGTGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGACT1140.14156215075127282No Hit
GTCGGTGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA1090.13535328449025208No Hit
GTCGGTGGGATAAATAAAATTTAGGAACAAAACCGGTGGTTTATCCTTGCATCTCTGC1060.13162796473363964No Hit
GTCGGTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA1040.12914441822923134No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCGGA1020.12666087172482304No Hit
GTCGGTGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1010.1254190984726189No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCCTC1010.1254190984726189No Hit
GTCGGTGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA1010.1254190984726189No Hit
GTCGGTGGGGCTTACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCT1010.1254190984726189No Hit
GTCGGTGGGGGAGAAGGTACCATGAGGTGCCTTTTGTTTTAGGATGAAATATTCCATA1000.12417732522041476No Hit
GTCGGTGGGATGGACAGCCATAGAACGTTATGAAATGTTTAAATTTAAAATGTTTTAA970.1204520054638023No Hit
GTCGGTGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC970.1204520054638023No Hit
GTCGGTGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTCCA960.11921023221159817No Hit
GTCGGTGGGGGGCTGGAGGAATGGCTCAGTGGTTAAGAGCACTGACTGCTTGTCCAGA960.11921023221159817No Hit
GTCGGTGGGGGAAAAATTTTTCACAATAATTTTTCCACCATAAATTGGATATAGTGCA940.11672668570718986No Hit
GTCGGTGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAG930.11548491245498572No Hit
GTCGGTGGGAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGTGATGG930.11548491245498572No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG910.11300136595057743No Hit
GTCGGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA910.11300136595057743No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT880.10927604619396498No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC880.10927604619396498No Hit
GTCGGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCC880.10927604619396498No Hit
GTCGGTGGGGGGGCTGGAGGAATGGCTCAGTGGTTAAGAGCACTGACTGCTTGTCCAG870.10803427294176084No Hit
GTCGGTGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC870.10803427294176084No Hit
GTCGGTGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGTGTTCA860.10679249968955667No Hit
GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTC860.10679249968955667No Hit
GTCGGTGGGGGGGCTGGAGATATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTGCGA850.10555072643735255No Hit
GTCGGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA850.10555072643735255No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTCCG830.10306717993294424No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTTAGTGGGTAAAAGCACCCGACTGCTCTTCCG820.10182540668074008No Hit
GTCGGTGGGGGGACTAGAGAGATGGCTCAGTGGTTAAGAGCATTGACTGCTCTTCCAA820.10182540668074008No Hit
GTCGGTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGGAGCTAA820.10182540668074008No Hit
GTCGGTGGGGCCTTTTTTTCCCTCAGCGCTGGCCGCGGCCTCCCGGCGCTCTCGTCCT810.10058363342853596No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAAA203.3190785E-452.3419088
GAACATT251.578782E-552.3419089
AGTCTTA550.052.34190810
GGCATGC150.00701156452.3419048
GGACTAA150.00701156452.3419048
GATAAAT150.00701156452.3419049
GGTTCGT150.00701156452.34190410
GCGTGAA150.00701156452.34190410
GACGCTC150.00701156452.34190410
GCATGCC150.00701156452.3419049
GGGATAA307.5515527E-752.3419047
GGCTAAT150.00701156452.3419048
TGGGTAC307.5515527E-752.3419046
GGAGTAT150.00701156452.3419048
CATGCCA150.00701156452.34190410
GGACGCT150.00701156452.3419049
GGGCTAA150.00701156452.3419047
ATCTCGA203.4022293E-452.0805339
CGAACTG203.4022293E-452.0805339
CAAAGAC203.4022293E-452.0805338
Produced by FastQC (version 0.11.5)