FastQCFastQC Report
Tue 31 May 2016
SRR212549_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212549_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105863
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC20061.8949019015142212No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC17151.6200183255717295No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC14071.3290762589384393No Hit
GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC14061.3281316418389806No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC12951.223279143799061No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC10320.974844846641414No Hit
GTCGGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA9690.9153339693755137No Hit
GTCGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT7110.6716227577151601No Hit
GTCGGTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC6800.6423396276319394No Hit
GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC6760.6385611592341045No Hit
GTCGGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA5950.5620471741779469No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC5750.5431548321887723No Hit
GTCGGTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC5310.501591679812588No Hit
GTCGGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA4730.44680388804398136No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4200.3967391817726685No Hit
GTCGGTGGGACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4140.391071479175916No Hit
GTCGGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG3790.35800988069486034No Hit
GTCGGTGGGAGTCTTAACATACATGTAGCAGATTGTATGTCATAGGGTACTTCTGT3700.34950832679973176No Hit
GTCGGTGGGATTGCAGATATCTGATGGATGTAGTTTTCTTTTTTTAAATTAGAAAT3630.3428960071035206No Hit
GTCGGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA3460.3268375164127221No Hit
GTCGGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG2940.2777174272408679No Hit
GTCGGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2770.26165893655006944No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT2760.2607143194506107No Hit
GTCGGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG2740.25882508525169323No Hit
GTCGGTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT2710.255991233953317No Hit
GTCGGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA2310.21820654997496766No Hit
GTCGGTGGGGAGCCAGCCCCAGAGAGGTGAGAGGAAGTGGTGTCAAGGGCTTTGCC2270.2144280815771327No Hit
GTCGGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC2230.21064961317929778No Hit
GTCGGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2200.20781576188092157No Hit
GTCGGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT2170.20498191058254533No Hit
GTCGGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGGGGGCCCAAGAAACA2170.20498191058254533No Hit
GTCGGTGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT2120.20025882508525167No Hit
GTCGGTGGGATCACGTTCTCCTGTGGGCCAGTATTGTGGAACGAGTCTGAGTTGTT2090.19742497378687549No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCC2080.19648035668741676No Hit
GTCGGTGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT1970.18608956859337067No Hit
GTCGGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA1860.17569878049932458No Hit
GTCGGTGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT1840.17380954630040713No Hit
GTCGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.15869567270906737No Hit
GTCGGTGGGAATTTGGAAGGGTGGAAATGATGTAAATATAGTATTCATGTATGAAA1640.15491720431123243No Hit
GTCGGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1620.15302797011231498No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1620.15302797011231498No Hit
GTCGGTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGGCCACGCCGAGCGAC1600.1511387359133975No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCC1580.14924950171448004No Hit
GTCGGTGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1540.1454710333166451No Hit
GTCGGTGGGACCATTTTTGTGGGTGTATATATATATTTGACTTGGGGAGAATTATT1490.14074794781935143No Hit
GTCGGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG1390.13130177682476407No Hit
GTCGGTGGGATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGC1370.12941254262584662No Hit
GTCGGTGGGGAATATAGATTGTAGTGTGTGGTTCTCTTTTGAAATTTTTTTCAGGT1300.12280022292963547No Hit
GTCGGTGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC1290.12185560583017675No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGCTCTTCC1290.12185560583017675No Hit
GTCGGTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1270.11996637163125927No Hit
GTCGGTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCTC1250.11807713743234179No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTTAGTGGGTAAAAGCACCCGACTGCTCTTC1230.11618790323342433No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTC1230.11618790323342433No Hit
GTCGGTGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG1220.11524328613396559No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTC1200.11335405193504812No Hit
GTCGGTGGGGAATACCAATTTTAAAGTTTCTGAAGAATTGCTAAGAGTCAGAGGAA1190.1124094348355894No Hit
GTCGGTGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGA1190.1124094348355894No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTC1190.1124094348355894No Hit
GTCGGTGGGGGAGAAGGTACCATGAGGTGCCTTTTGTTTTAGGATGAAATATTCCA1180.11146481773613066No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCG1170.11052020063667192No Hit
GTCGGTGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGGGG1130.10674173223883698No Hit
GTCGGTGGGGACACCATGCCAAGTCAGTTTCTTCCACAGCAGGCCACCTACTTTCC1100.10390788094046079No Hit
GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCC1090.10296326384100206No Hit
GTCGGTGGGATGGACAGCCATAGAACGTTATGAAATGTTTAAATTTAAAATGTTTT1090.10296326384100206No Hit
GTCGGTGGGGCCTTTTTTTCCCTCAGCGCTGGCCGCGGCCTCCCGGCGCTCTCGTC1070.10107402964208458No Hit
GTCGGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC1070.10107402964208458No Hit
GTCGGTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGGGGTCCACGCCGAGCGAC1070.10107402964208458No Hit
GTCGGTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1060.10012941254262583No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCGT150.00828496850.1896449
AGTTGTT150.00828496850.1896450
ATGAGAG150.00828496850.1896449
TAGAAAT507.2759576E-1250.18963650
TTTTTAC204.0893536E-450.18963650
ACGACTC204.0893536E-450.18963650
TTGTGCA252.028512E-550.18963650
CTACTTT204.0893536E-450.18963648
CTATTAT355.0744347E-850.18963648
TATACTA355.093534E-850.165844
TTATACT355.093534E-850.165843
ATTATAC355.093534E-850.165842
ATACTAT355.093534E-850.165845
TACTATT355.093534E-850.165846
ACTATTA355.093534E-850.165847
CGCGACC204.0989608E-450.16579442
GTCACGG204.0989608E-450.16579442
GAAACCC150.00830055350.16579446
AAATTAG507.2759576E-1250.16579446
CCAAATC150.00830055350.16579446