SRR212549_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212549_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	105863
Sequences flagged as poor quality	0
Sequence length	56
%GC	57

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	2006	1.8949019015142212	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	1715	1.6200183255717295	No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	1407	1.3290762589384393	No Hit
GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	1406	1.3281316418389806	No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	1295	1.223279143799061	No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC	1032	0.974844846641414	No Hit
GTCGGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	969	0.9153339693755137	No Hit
GTCGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	711	0.6716227577151601	No Hit
GTCGGTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC	680	0.6423396276319394	No Hit
GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC	676	0.6385611592341045	No Hit
GTCGGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	595	0.5620471741779469	No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC	575	0.5431548321887723	No Hit
GTCGGTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC	531	0.501591679812588	No Hit
GTCGGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	473	0.44680388804398136	No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	420	0.3967391817726685	No Hit
GTCGGTGGGACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	414	0.391071479175916	No Hit
GTCGGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	379	0.35800988069486034	No Hit
GTCGGTGGGAGTCTTAACATACATGTAGCAGATTGTATGTCATAGGGTACTTCTGT	370	0.34950832679973176	No Hit
GTCGGTGGGATTGCAGATATCTGATGGATGTAGTTTTCTTTTTTTAAATTAGAAAT	363	0.3428960071035206	No Hit
GTCGGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA	346	0.3268375164127221	No Hit
GTCGGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	294	0.2777174272408679	No Hit
GTCGGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	277	0.26165893655006944	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT	276	0.2607143194506107	No Hit
GTCGGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG	274	0.25882508525169323	No Hit
GTCGGTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	271	0.255991233953317	No Hit
GTCGGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	231	0.21820654997496766	No Hit
GTCGGTGGGGAGCCAGCCCCAGAGAGGTGAGAGGAAGTGGTGTCAAGGGCTTTGCC	227	0.2144280815771327	No Hit
GTCGGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC	223	0.21064961317929778	No Hit
GTCGGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	220	0.20781576188092157	No Hit
GTCGGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	217	0.20498191058254533	No Hit
GTCGGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGGGGGCCCAAGAAACA	217	0.20498191058254533	No Hit
GTCGGTGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT	212	0.20025882508525167	No Hit
GTCGGTGGGATCACGTTCTCCTGTGGGCCAGTATTGTGGAACGAGTCTGAGTTGTT	209	0.19742497378687549	No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCC	208	0.19648035668741676	No Hit
GTCGGTGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT	197	0.18608956859337067	No Hit
GTCGGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	186	0.17569878049932458	No Hit
GTCGGTGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT	184	0.17380954630040713	No Hit
GTCGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	168	0.15869567270906737	No Hit
GTCGGTGGGAATTTGGAAGGGTGGAAATGATGTAAATATAGTATTCATGTATGAAA	164	0.15491720431123243	No Hit
GTCGGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	162	0.15302797011231498	No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	162	0.15302797011231498	No Hit
GTCGGTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGGCCACGCCGAGCGAC	160	0.1511387359133975	No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCC	158	0.14924950171448004	No Hit
GTCGGTGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	154	0.1454710333166451	No Hit
GTCGGTGGGACCATTTTTGTGGGTGTATATATATATTTGACTTGGGGAGAATTATT	149	0.14074794781935143	No Hit
GTCGGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG	139	0.13130177682476407	No Hit
GTCGGTGGGATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGC	137	0.12941254262584662	No Hit
GTCGGTGGGGAATATAGATTGTAGTGTGTGGTTCTCTTTTGAAATTTTTTTCAGGT	130	0.12280022292963547	No Hit
GTCGGTGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC	129	0.12185560583017675	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGCTCTTCC	129	0.12185560583017675	No Hit
GTCGGTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	127	0.11996637163125927	No Hit
GTCGGTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCTC	125	0.11807713743234179	No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTTAGTGGGTAAAAGCACCCGACTGCTCTTC	123	0.11618790323342433	No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTC	123	0.11618790323342433	No Hit
GTCGGTGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG	122	0.11524328613396559	No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTC	120	0.11335405193504812	No Hit
GTCGGTGGGGAATACCAATTTTAAAGTTTCTGAAGAATTGCTAAGAGTCAGAGGAA	119	0.1124094348355894	No Hit
GTCGGTGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGA	119	0.1124094348355894	No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTC	119	0.1124094348355894	No Hit
GTCGGTGGGGGAGAAGGTACCATGAGGTGCCTTTTGTTTTAGGATGAAATATTCCA	118	0.11146481773613066	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCG	117	0.11052020063667192	No Hit
GTCGGTGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGGGG	113	0.10674173223883698	No Hit
GTCGGTGGGGACACCATGCCAAGTCAGTTTCTTCCACAGCAGGCCACCTACTTTCC	110	0.10390788094046079	No Hit
GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCC	109	0.10296326384100206	No Hit
GTCGGTGGGATGGACAGCCATAGAACGTTATGAAATGTTTAAATTTAAAATGTTTT	109	0.10296326384100206	No Hit
GTCGGTGGGGCCTTTTTTTCCCTCAGCGCTGGCCGCGGCCTCCCGGCGCTCTCGTC	107	0.10107402964208458	No Hit
GTCGGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC	107	0.10107402964208458	No Hit
GTCGGTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGGGGTCCACGCCGAGCGAC	107	0.10107402964208458	No Hit
GTCGGTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	106	0.10012941254262583	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCCCGT	15	0.008284968	50.18964	49
AGTTGTT	15	0.008284968	50.18964	50
ATGAGAG	15	0.008284968	50.18964	49
TAGAAAT	50	7.2759576E-12	50.189636	50
TTTTTAC	20	4.0893536E-4	50.189636	50
ACGACTC	20	4.0893536E-4	50.189636	50
TTGTGCA	25	2.028512E-5	50.189636	50
CTACTTT	20	4.0893536E-4	50.189636	48
CTATTAT	35	5.0744347E-8	50.189636	48
TATACTA	35	5.093534E-8	50.1658	44
TTATACT	35	5.093534E-8	50.1658	43
ATTATAC	35	5.093534E-8	50.1658	42
ATACTAT	35	5.093534E-8	50.1658	45
TACTATT	35	5.093534E-8	50.1658	46
ACTATTA	35	5.093534E-8	50.1658	47
CGCGACC	20	4.0989608E-4	50.165794	42
GTCACGG	20	4.0989608E-4	50.165794	42
GAAACCC	15	0.008300553	50.165794	46
AAATTAG	50	7.2759576E-12	50.165794	46
CCAAATC	15	0.008300553	50.165794	46

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content