##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212549_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 105863 Sequences flagged as poor quality 0 Sequence length 56 %GC 57 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.37045993406573 38.0 35.0 38.0 33.0 38.0 2 35.16114223099667 38.0 34.0 38.0 29.0 38.0 3 35.97138754805739 38.0 35.0 38.0 32.0 38.0 4 35.980181933253355 38.0 35.0 38.0 32.0 38.0 5 36.03069060956141 38.0 35.0 38.0 32.0 38.0 6 32.6026184785997 35.0 31.0 36.0 23.0 38.0 7 37.022056809272364 38.0 37.0 38.0 34.0 39.0 8 36.979549039796716 38.0 37.0 38.0 34.0 39.0 9 35.09269527596989 35.0 35.0 38.0 30.0 39.0 10 33.91940526907418 35.0 32.0 38.0 28.0 39.0 11 34.520304544552864 36.0 33.0 39.0 28.0 39.0 12 35.00653675032825 37.0 34.0 39.0 28.0 39.0 13 35.06720950662649 37.0 34.0 38.0 29.0 39.0 14 35.29778109443337 38.0 34.0 38.0 29.0 39.0 15 35.495631145915006 38.0 35.0 38.0 29.0 39.0 16 35.739833558467076 38.0 35.0 39.0 30.0 39.0 17 35.31916722556512 38.0 35.0 38.0 29.0 39.0 18 35.095245742138424 38.0 33.0 38.0 29.0 39.0 19 34.8602155616221 37.0 33.0 38.0 29.0 39.0 20 34.58200693348951 37.0 33.0 38.0 28.0 39.0 21 34.30615984810557 36.0 33.0 38.0 27.0 39.0 22 34.06550919584746 36.0 33.0 38.0 27.0 39.0 23 33.86978453283962 36.0 33.0 38.0 27.0 39.0 24 33.24636558570983 36.0 32.0 38.0 25.0 39.0 25 33.16874639864731 36.0 32.0 38.0 25.0 39.0 26 32.210063950577634 35.0 31.0 38.0 23.0 39.0 27 31.95108772659003 35.0 30.0 38.0 22.0 39.0 28 31.366331957341092 35.0 29.0 38.0 19.0 38.0 29 31.243220010768635 35.0 29.0 38.0 18.0 38.0 30 30.923183737472016 35.0 29.0 38.0 17.0 38.0 31 29.3802745057291 33.0 27.0 37.0 9.0 39.0 32 29.47877917686066 33.0 27.0 37.0 8.0 38.0 33 29.193939336689873 33.0 27.0 37.0 2.0 38.0 34 28.343972870596904 33.0 25.0 36.0 2.0 38.0 35 27.982590706856975 32.0 24.0 36.0 2.0 38.0 36 28.609221352124916 33.0 26.0 38.0 2.0 39.0 37 28.365869094962356 33.0 26.0 37.0 2.0 39.0 38 27.954545025174045 33.0 25.0 36.0 2.0 39.0 39 27.668817244929766 33.0 24.0 37.0 2.0 39.0 40 27.57441221200986 33.0 24.0 36.0 2.0 39.0 41 26.60846565844535 33.0 21.0 36.0 2.0 39.0 42 25.754579031389625 32.0 16.0 36.0 2.0 39.0 43 25.931316890698355 32.0 18.0 36.0 2.0 39.0 44 25.783937730840805 32.0 17.0 36.0 2.0 39.0 45 25.062977622020913 31.0 10.0 36.0 2.0 39.0 46 23.87884341082342 31.0 2.0 36.0 2.0 39.0 47 23.78230354325874 30.0 2.0 35.0 2.0 39.0 48 23.659191596686284 30.0 2.0 35.0 2.0 39.0 49 23.112466111861558 30.0 2.0 35.0 2.0 38.0 50 22.724304053351975 30.0 2.0 35.0 2.0 38.0 51 21.94024352228824 30.0 2.0 35.0 2.0 38.0 52 21.617146689589376 30.0 2.0 35.0 2.0 38.0 53 21.191473886060287 29.0 2.0 35.0 2.0 38.0 54 20.823394387085195 29.0 2.0 35.0 2.0 38.0 55 20.433012478391884 29.0 2.0 35.0 2.0 38.0 56 11.739417926943313 15.0 2.0 21.0 2.0 24.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.0202590074056062 1 2 -2.109095911702191 1 3 -2.2214551746408375 1 4 -1.6748079904827335 1 5 -1.2289098678653758 1 6 -2.5065909804715147 1 7 -2.844859481375451 1 8 -3.148403315620925 1 9 -3.3884894604497866 1 10 -2.580467297154776 1 11 -1.7664685522094814 1 12 -1.8800733893981487 1 13 -2.2729192736094674 1 14 -1.2166043862687772 1 15 -2.5676776956720175 1 16 -2.0143784179861655 1 17 -3.2190470750678912 1 18 -2.5191638350152488 1 19 -2.1714469150153093 1 20 -2.3244673990393707 1 21 -1.9520938910319217 1 22 -2.409022661627656 1 23 -2.502761820760444 1 24 -2.015464644273173 1 25 -1.4824235625102347 1 26 -2.786840617805204 1 27 -0.8485141576505093 1 28 -0.7408751948881225 1 29 -1.2329422256889586 1 30 -2.053476425503966 1 31 -0.35759896687613946 1 32 -0.8609269215416688 1 33 0.42020516685710163 1 34 -0.06727427193504099 1 35 0.06107203096199143 1 36 -0.6685289084494563 1 37 -1.8404043438003903 1 38 -0.36992798518510384 1 39 -1.3152424170733603 1 40 -0.1372502063019816 1 41 -1.027308365305597 1 42 -0.8697050080688911 1 43 -0.050942602999903386 1 44 -0.8390561860569328 1 45 0.7988955955927572 1 46 0.38574537684844046 1 47 -0.9316163861058442 1 48 -0.7381502469780443 1 49 0.04975918354084996 1 50 -1.4864154596474322 1 51 -1.3390524283426721 1 52 -1.8424881286481494 1 53 -3.018483392986404 1 54 -1.5467769131485092 1 55 -3.1782404688636845 1 56 -0.45823324606458193 2 1 -0.9371217525036499 2 2 -1.2032135587610142 2 3 -2.129690468758483 2 4 -0.9595138728356716 2 5 -0.9685177110026331 2 6 -2.148159607922498 2 7 -1.2001535990225065 2 8 -0.7601680215032758 2 9 -1.571234558489003 2 10 -1.0181143559783052 2 11 -1.2998018855428128 2 12 -1.1059557423393258 2 13 -0.43135064615849217 2 14 -0.8479769352883864 2 15 -0.3378737741033859 2 16 -1.7532019473979332 2 17 -0.49904707506789237 2 18 -1.5011246193289765 2 19 -0.28674103266236983 2 20 -0.3291732813923147 2 21 -0.7081723224044723 2 22 0.018428318764499352 2 23 -0.28001672272122846 2 24 -0.8429156246653307 2 25 0.148164672783885 2 26 -0.4189974805503027 2 27 -1.1465533733367828 2 28 -2.1863653909665537 2 29 -1.3992167354928782 2 30 -1.9844568176608277 2 31 -3.3474028884447655 2 32 -2.655436725463236 2 33 -3.7162654213781927 2 34 -2.541784075856608 2 35 -2.487947576881144 2 36 -1.939117143743573 2 37 -1.0788357163494098 2 38 -1.7769868087145184 2 39 -1.8454384955047338 2 40 -1.2972502063019817 2 41 -2.7708377770703017 2 42 -1.5465677531669328 2 43 -2.209373975548921 2 44 -2.024938538998107 2 45 -1.5454181298974383 2 46 -1.4322938388378326 2 47 -2.293969327282312 2 48 -1.8299149528603955 2 49 -1.1102408164591502 2 50 -1.31857232239253 2 51 -1.3304249773622807 2 52 -1.8322920502167754 2 53 -2.028679471417778 2 54 -0.7883455405994901 2 55 -2.124122821804864 2 56 -1.8998018735155622 3 1 -1.1135923407389399 3 2 -1.8979848005910824 3 3 -1.3636773968630607 3 4 -1.0836968793716224 3 5 -0.8489098678653733 3 6 -1.1821465360270729 3 7 -0.5204150369310057 3 8 -0.4261810933986965 3 9 -0.9862672382275619 3 10 -1.167133963821442 3 11 -0.04424632998725997 3 12 -0.23340672273148044 3 13 -0.2973637180539157 3 14 -0.18327105293544577 3 15 -0.43656658456090724 3 16 -0.8721561957639423 3 17 -1.010158186178998 3 18 -0.5991638350152471 3 19 -0.784780248348639 3 20 -0.8644673990393699 3 21 -0.20098277992081393 3 22 0.035421782816790426 3 23 -0.3872062652048882 3 24 -0.648797977606506 3 25 -0.45575689584357093 3 26 -0.20461839558298323 3 27 -0.45962526876162 3 28 -0.3275418615547885 3 29 0.8092799965332667 3 30 0.777634685607147 3 31 1.2957343664571965 3 32 1.9524064117916637 3 33 2.671316277968213 3 34 1.8438368391760704 3 35 2.3721831420731014 3 36 1.9870266471060987 3 37 1.9662623228662781 3 38 2.0589609037037846 3 39 2.022535360704417 3 40 1.8694164603646861 3 41 2.3526916346944056 3 42 3.4436283252644415 3 43 2.802390730333432 3 44 2.9587215917208454 3 45 2.616673373370535 3 46 3.332412043515106 3 47 3.555050280560824 3 48 4.659627530799735 3 49 2.7008702946519634 3 50 3.8958067625747894 3 51 3.6009475716573256 3 52 3.0997340935740745 3 53 3.6504054959024863 3 54 2.7443341979626013 3 55 3.2150928644696464 3 56 1.110655642824307 4 1 -0.4838051066963871 4 2 -0.22611718829793404 4 3 -0.6116679405982808 4 4 -0.47565905431251565 4 5 -0.43259781112778484 4 6 -0.5386477180601688 4 7 -0.2308169281839625 4 8 -0.12925437945070684 4 9 -0.3822483256980149 4 10 -0.18770133970797076 4 11 -0.7763976302236699 4 12 -0.8617755170577226 4 13 -0.4876710466591163 4 14 -0.838590201871618 4 15 -0.3760464899982594 4 16 -0.7582082052202068 4 17 -0.4373449474083131 4 18 -1.2679581612563808 4 19 -1.2025107448025452 4 20 -0.5325525054223519 4 21 -0.3574839619539105 4 22 -0.26930634957091826 4 23 -1.0541093384909388 4 24 -0.8828405307979921 4 25 -0.8351895199570407 4 26 0.6164217935423153 4 27 -0.29248578885618315 4 28 0.023521968232444124 4 29 -0.2356372611499502 4 30 0.9842540709499339 4 31 -1.6794429385073428 4 32 -1.21638791444947 4 33 -1.554688450164175 4 34 -1.4537990946300745 4 35 -0.6013393165557375 4 36 -2.0515076318537133 4 37 -0.7436667551479061 4 38 -2.3001407511425533 4 39 -1.2368027007613058 4 40 -0.4844842488551713 4 41 -0.9281594291353841 4 42 -0.1677191924660555 4 43 -0.2316518228580584 4 44 0.4000927501132807 4 45 0.08073956722396147 4 46 0.08134821372787471 4 47 0.43519212453245615 4 48 -0.21176726825463987 4 49 0.062099609072763684 4 50 -0.06187645255523577 4 51 0.00449367094810782 4 52 0.2611998146142618 4 53 0.8076158977937382 4 54 0.10627273224156042 4 55 1.0200574034767413 4 56 1.2905610801765537 5 1 -0.17241587015070792 5 2 0.22031585300369017 5 3 -0.10616105699377698 5 4 0.2522508330466806 5 5 -0.15675300512027945 5 6 0.5341933332539739 5 7 -0.16485948137545137 5 8 -0.34840331562092075 5 9 -0.17123455848900448 5 10 -0.13576141480183423 5 11 0.24137458504542053 5 12 1.1175736694253828 5 13 0.4392375891356295 5 14 1.1520230647116136 5 15 0.7444791670730808 5 16 0.5526804055432493 5 17 0.12448233669681485 5 18 1.1341694983180872 5 19 -0.21615279736825244 5 20 0.04729730684297806 5 21 -0.319937028286823 5 22 0.37136949523509344 5 23 -0.6211931933094661 5 24 -0.5840920952535669 5 25 -0.13418826839259168 5 26 1.2633554606261654 5 27 0.041681920780863635 5 28 0.1430463737393275 5 29 1.4831362056835893 5 30 -0.0785744647196509 5 31 1.1584794644964127 5 32 0.8739750392426444 5 33 1.1425581080335725 5 34 1.5170394535551566 5 35 1.8414641878247373 5 36 1.8373534444917219 5 37 1.356458401297651 5 38 0.10536613246195259 5 39 0.6133850339070293 5 40 1.0792203819333146 5 41 1.8409269288120527 5 42 2.3122557762448324 5 43 0.9788613185687254 5 44 1.198590872766598 5 45 1.113405399514324 5 46 1.7559414552798138 5 47 1.811913025658864 5 48 1.7347909294925472 5 49 0.7603474188349679 5 50 1.6931923834898228 5 51 1.6695750226377193 5 52 2.6382961850773405 5 53 3.2183793521116364 5 54 2.4587132829299208 5 55 2.1347007076069033 5 56 1.5001981264844382 6 1 0.27529654814994586 6 2 0.1353485327422561 6 3 -0.13034406352972638 6 4 0.2940808984061576 6 5 0.25109013213462816 6 6 1.173409019528485 6 7 0.23514051862455432 6 8 0.06270779549019068 6 9 0.33595498399466095 6 10 -0.4560228527103334 6 11 -0.35535744109837 6 12 0.21103772171296242 6 13 0.06930294861275144 6 14 -0.10549327515767004 6 15 -0.7810110290053487 6 16 -0.47215619576394374 6 17 -1.8101581861790024 6 18 -0.7991638350152499 6 19 -0.6736691372375319 6 20 -0.47557851015048414 6 21 -0.2676494465874768 6 22 0.124310671705679 6 23 -0.7538729318715554 6 24 -1.0043535331620603 6 25 0.010909770823097631 6 26 -0.5712850622496504 6 27 -0.11518082431717502 6 28 -0.7386529726658999 6 29 -0.024053336800069047 6 30 -0.11125420328174229 6 31 1.4512899220127515 6 32 1.2968508562361087 6 33 1.193538500190435 6 34 2.154947950287184 6 35 1.7499609198508814 6 36 2.0981377582172094 6 37 1.8884845450884988 6 38 1.8034053481482282 6 39 1.3780909162599713 6 40 1.558305349253576 6 41 1.4971360791388477 6 42 2.0991838808199965 6 43 2.2135018414445433 6 44 1.0587215917208468 6 45 1.8833400400372007 6 46 2.043523154626218 6 47 2.577272502783046 6 48 2.3707386419108474 6 49 3.045314739096405 6 50 2.4402512070192337 6 51 3.0787253494351035 6 52 3.1775118713518538 6 53 3.739294384791375 6 54 2.855445309073712 6 55 3.070648420025204 6 56 1.1662111983798624 7 1 -0.13381705984006942 7 2 0.12685914447758506 7 3 0.03607291524680534 7 4 0.1377762791801871 7 5 0.13947964524324163 7 6 -0.5486883587486702 7 7 0.16997197929870822 7 8 0.1639562349408763 7 9 0.26154799273373186 7 10 0.4703192196991566 7 11 1.4327823841201024 7 12 0.6066681836355627 7 13 0.991650014780042 7 14 0.6695379358286004 7 15 0.94632979496469 7 16 0.6089923685306857 7 17 0.4649978687523344 7 18 0.14765264438550219 7 19 0.5979912872318849 7 20 1.109689904331411 7 21 -0.07538977118174017 7 22 1.0231870762000597 7 23 0.05574005189873077 7 24 -0.08138224726942411 7 25 -0.1786033377911309 7 26 -0.07028631068909874 7 27 1.4765794753082915 7 28 0.032008700242965205 7 29 0.8323761263709706 7 30 0.6163363085784326 7 31 0.7526632054085063 7 32 0.7891105316418532 7 33 1.6579579758458678 7 34 0.35220138349567165 7 35 0.8371019935088064 7 36 1.5786621027864989 7 37 1.8924795513306982 7 38 2.3981619024553424 7 39 1.380338107271207 7 40 1.3814014790912772 7 41 1.9605568032337288 7 42 2.425276265339349 7 43 3.0473345505581513 7 44 2.092179768999248 7 45 2.963240164881146 7 46 2.3144345154252193 7 47 3.067035299287415 7 48 2.9009508766174648 7 49 2.532455812754332 7 50 2.8144085103900203 7 51 2.059000006114257 7 52 2.62732460543425 7 53 2.8308049965267053 7 54 2.3369684052035495 7 55 2.145804474956538 7 56 2.4592199374560177 8 1 0.04196321481661158 8 2 -0.08687368947996532 8 3 0.25854482535916645 8 4 0.21630312062838186 8 5 0.1177567988012882 8 6 -1.2821465360270707 8 7 -0.08708170359766854 8 8 -0.39284776006536504 8 9 -0.33071168267201045 8 10 -0.21157840826588625 8 11 -0.15535744109837424 8 12 -0.37785116717592615 8 13 -0.20847482916502713 8 14 0.58339561373122 8 15 0.0967667487724313 8 16 -0.32771175131949803 8 17 -0.2990470750678895 8 18 -0.28805272390413705 8 19 -0.6847802483486447 8 20 0.16886593429396157 8 21 -0.6898716688097011 8 22 -0.809022661627651 8 23 0.023904845906223215 8 24 -0.837686866495396 8 25 -0.2779791180657938 8 26 0.08427049330590819 8 27 -0.05962526876162144 8 28 -0.33865297266590133 8 29 0.4092799965332645 8 30 -0.8556986477261859 8 31 0.37351214423497225 8 32 0.9301841895694416 8 33 0.02687183352377076 8 34 0.6105035058427397 8 35 0.8166275865175479 8 36 0.7648044248838772 8 37 0.5107067673107224 8 38 0.0034053481482274606 8 39 0.3225353607044177 8 40 0.4583053492535747 8 41 1.1638027458055156 8 42 1.010294991931108 8 43 1.424612952555652 8 44 0.8587215917208475 8 45 1.5166733733705335 8 46 2.599078710181775 8 47 1.9772725027830447 8 48 2.3929608641330695 8 49 2.8564258502075184 8 50 2.31802898479701 8 51 2.912058682768439 8 52 2.9886229824629638 8 53 1.5948499403469292 8 54 2.355445309073712 8 55 0.8595373089140921 8 56 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4 44.0 5 62.0 6 95.0 7 166.0 8 114.0 9 200.0 10 316.0 11 389.0 12 565.0 13 757.0 14 950.0 15 1110.0 16 1348.0 17 1556.0 18 1682.0 19 1972.0 20 1978.0 21 1937.0 22 2235.0 23 2537.0 24 2847.0 25 3050.0 26 3103.0 27 3331.0 28 3802.0 29 4333.0 30 5056.0 31 6039.0 32 7272.0 33 8569.0 34 9507.0 35 9680.0 36 9631.0 37 7459.0 38 1862.0 39 150.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 62.95495121052681 20.77118539999811 4.047684271180677 12.226179118294398 11 29.651530752009673 17.004997024456138 16.43444829638306 36.90902392715113 12 31.321613783852715 10.43518509772064 36.75599595703881 21.48720516138783 13 19.80200825595345 13.189688559742308 36.90902392715113 30.09927925715311 14 15.38308946468549 16.156730869142194 49.2296647553914 19.230514910780915 15 22.267458885540748 12.4047117500921 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23.890675241157556 20.123888783809342 24.188575751844148 29 31.102891092106262 17.895254673427686 22.226216604858852 28.775637629607203 30 25.654955705307792 19.160293783599606 25.725940788975542 29.458809722117056 31 36.13545590490072 16.423744534252023 24.833898048420373 22.606901512426887 32 40.0844561008171 13.865192156565703 25.54797049717375 20.50238124544344 33 43.16106543401659 13.305611336339181 23.78187398173758 19.751449247906642 34 30.52226716812399 17.87531143720573 35.01738364327059 16.585037751399692 35 25.511896076331976 28.144098390168566 32.35296904461858 13.991036488880887 36 34.462821667219565 27.98824700251173 23.24629164494574 14.302639685322971 37 30.751874709668858 27.85472540598959 23.481508868727662 17.91189101561389 38 31.877021119214028 24.329296626805373 23.906343350814137 19.88733890316646 39 30.75456666476982 25.398812572317382 25.238528803657122 18.608091959255677 40 33.72043378020664 20.015370164802324 23.621666239717644 22.64252981527339 41 34.19671913395763 19.751610546589625 25.945217696562583 20.10645262289016 42 26.511720641430976 24.291966922061768 27.106060174504165 22.090252262003098 43 32.20578741503057 16.896099950632287 25.212660919758477 25.685451714578665 44 26.998006266021076 14.344441279787334 24.997626507167947 33.65992594702364 45 32.35313672412975 16.81056180842475 25.285653226955407 25.550648240490098 46 28.847706526591754 19.47053962403944 27.6451646608471 24.03658918852171 47 30.682541643307136 18.201427227548724 27.049857942397782 24.066173186746358 48 31.89432254629806 16.17009544814998 28.242765334448794 23.692816671103166 49 25.6913766732218 18.90253750201879 26.78674912835713 28.619336696402275 50 20.911709074318736 17.949034778393482 29.950859717324562 31.188396429963216 51 20.451736791071735 17.23102078065289 36.09236268228226 26.224879745993118 52 19.819879793061475 18.32204808277541 38.37492391965916 23.483148204503955 53 17.7935875364993 20.290282388076736 35.42928884619408 26.486841229229878 54 20.722923663904243 19.383259911894275 36.986327437417344 22.90748898678414 55 19.587717128669546 17.536766804284547 29.630334278267156 33.24518178877875 56 19.30880968008904 19.634142861219715 24.88893962310817 36.16810783558308 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 31.5 13 75.5 14 88.0 15 86.5 16 85.0 17 115.0 18 145.0 19 237.5 20 330.0 21 392.0 22 507.0 23 560.0 24 587.0 25 614.0 26 800.0 27 986.0 28 1431.0 29 1771.5 30 1667.0 31 1970.5 32 2274.0 33 2040.0 34 1806.0 35 1683.0 36 1560.0 37 1855.5 38 1645.5 39 1140.0 40 1126.0 41 1112.0 42 1238.5 43 1365.0 44 1379.0 45 1393.0 46 1599.0 47 1823.5 48 1842.0 49 1962.5 50 2083.0 51 2107.5 52 2132.0 53 2374.0 54 3351.5 55 4087.0 56 4084.0 57 4081.0 58 4231.5 59 4382.0 60 5972.5 61 7563.0 62 8210.5 63 9977.0 64 11096.0 65 11613.0 66 12130.0 67 10700.0 68 9270.0 69 7462.0 70 5654.0 71 4427.5 72 2524.0 73 1847.0 74 1633.5 75 1420.0 76 1117.5 77 815.0 78 677.5 79 422.5 80 305.0 81 264.0 82 223.0 83 178.5 84 134.0 85 96.0 86 58.0 87 40.0 88 21.5 89 21.0 90 13.0 91 5.0 92 4.0 93 3.0 94 2.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.022670810387009625 26 0.04534162077401925 27 0.08879400734912103 28 0.11618790323342433 29 0.15019411881393877 30 0.195535739587958 31 0.19364650538904055 32 0.23237580646684866 33 0.27488357594249174 34 0.2871635982354553 35 0.30605594022462995 36 0.3381729216062269 37 0.3589544977943191 38 0.391071479175916 39 0.40146226726996215 40 0.4383023341488527 41 0.4383023341488527 42 0.5063147653098817 43 0.501591679812588 44 0.5034809140115054 45 0.5459886834871485 46 0.5535456202828183 47 0.5894410700622503 48 0.6376165421346457 49 0.568659493874158 50 0.6177795830460123 51 0.6319488395378934 52 0.6716227577151601 53 0.6839027800081238 54 0.7197982297875556 55 0.6999612706989222 56 0.6990166535994635 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 105863.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.050858184634855 #Duplication Level Percentage of deduplicated Percentage of total 1 72.81167108753316 23.336765442128033 2 10.35366931918656 6.636879740797068 3 4.709696433834365 4.528494374805173 4 2.7232537577365163 3.4913047995994826 5 1.8331859711170055 2.937759179316664 6 1.308576480990274 2.5164599529580682 7 0.9313292071912761 2.0894930240027207 8 0.7603890362511052 1.949689693282828 9 0.6395520188623637 1.8448371952429081 >10 3.36280577659888 19.149277840227462 >50 0.3477748305334512 7.9640667655365895 >100 0.17978190391983495 10.71006867366313 >500 0.0206307102858827 4.474651200136025 >1k 0.01768346595932803 8.370252118303846 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 2006 1.8949019015142212 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1715 1.6200183255717295 No Hit GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 1407 1.3290762589384393 No Hit GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 1406 1.3281316418389806 No Hit GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 1295 1.223279143799061 No Hit GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 1032 0.974844846641414 No Hit GTCGGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 969 0.9153339693755137 No Hit GTCGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 711 0.6716227577151601 No Hit GTCGGTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC 680 0.6423396276319394 No Hit GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 676 0.6385611592341045 No Hit GTCGGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 595 0.5620471741779469 No Hit GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC 575 0.5431548321887723 No Hit GTCGGTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC 531 0.501591679812588 No Hit GTCGGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 473 0.44680388804398136 No Hit GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 420 0.3967391817726685 No Hit GTCGGTGGGACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 414 0.391071479175916 No Hit GTCGGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 379 0.35800988069486034 No Hit GTCGGTGGGAGTCTTAACATACATGTAGCAGATTGTATGTCATAGGGTACTTCTGT 370 0.34950832679973176 No Hit GTCGGTGGGATTGCAGATATCTGATGGATGTAGTTTTCTTTTTTTAAATTAGAAAT 363 0.3428960071035206 No Hit GTCGGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA 346 0.3268375164127221 No Hit GTCGGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 294 0.2777174272408679 No Hit GTCGGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 277 0.26165893655006944 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 276 0.2607143194506107 No Hit GTCGGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG 274 0.25882508525169323 No Hit GTCGGTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 271 0.255991233953317 No Hit GTCGGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 231 0.21820654997496766 No Hit GTCGGTGGGGAGCCAGCCCCAGAGAGGTGAGAGGAAGTGGTGTCAAGGGCTTTGCC 227 0.2144280815771327 No Hit GTCGGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC 223 0.21064961317929778 No Hit GTCGGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 220 0.20781576188092157 No Hit GTCGGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 217 0.20498191058254533 No Hit GTCGGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGGGGGCCCAAGAAACA 217 0.20498191058254533 No Hit GTCGGTGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT 212 0.20025882508525167 No Hit GTCGGTGGGATCACGTTCTCCTGTGGGCCAGTATTGTGGAACGAGTCTGAGTTGTT 209 0.19742497378687549 No Hit GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCC 208 0.19648035668741676 No Hit GTCGGTGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT 197 0.18608956859337067 No Hit GTCGGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 186 0.17569878049932458 No Hit GTCGGTGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT 184 0.17380954630040713 No Hit GTCGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 168 0.15869567270906737 No Hit GTCGGTGGGAATTTGGAAGGGTGGAAATGATGTAAATATAGTATTCATGTATGAAA 164 0.15491720431123243 No Hit GTCGGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 162 0.15302797011231498 No Hit GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 162 0.15302797011231498 No Hit GTCGGTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGGCCACGCCGAGCGAC 160 0.1511387359133975 No Hit GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCC 158 0.14924950171448004 No Hit GTCGGTGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 154 0.1454710333166451 No Hit GTCGGTGGGACCATTTTTGTGGGTGTATATATATATTTGACTTGGGGAGAATTATT 149 0.14074794781935143 No Hit GTCGGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG 139 0.13130177682476407 No Hit GTCGGTGGGATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGC 137 0.12941254262584662 No Hit GTCGGTGGGGAATATAGATTGTAGTGTGTGGTTCTCTTTTGAAATTTTTTTCAGGT 130 0.12280022292963547 No Hit GTCGGTGGGGGGGCTAGTGAGATGGCTCAATGGGTAAGAGCACCCGACTGCTCTTC 129 0.12185560583017675 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGCTCTTCC 129 0.12185560583017675 No Hit GTCGGTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 127 0.11996637163125927 No Hit GTCGGTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCTC 125 0.11807713743234179 No Hit GTCGGTGGGGGGGCTGGTGAGATGGCTTAGTGGGTAAAAGCACCCGACTGCTCTTC 123 0.11618790323342433 No Hit GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTC 123 0.11618790323342433 No Hit GTCGGTGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG 122 0.11524328613396559 No Hit GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTC 120 0.11335405193504812 No Hit GTCGGTGGGGAATACCAATTTTAAAGTTTCTGAAGAATTGCTAAGAGTCAGAGGAA 119 0.1124094348355894 No Hit GTCGGTGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGA 119 0.1124094348355894 No Hit GTCGGTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTC 119 0.1124094348355894 No Hit GTCGGTGGGGGAGAAGGTACCATGAGGTGCCTTTTGTTTTAGGATGAAATATTCCA 118 0.11146481773613066 No Hit GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCG 117 0.11052020063667192 No Hit GTCGGTGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGGGG 113 0.10674173223883698 No Hit GTCGGTGGGGACACCATGCCAAGTCAGTTTCTTCCACAGCAGGCCACCTACTTTCC 110 0.10390788094046079 No Hit GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCC 109 0.10296326384100206 No Hit GTCGGTGGGATGGACAGCCATAGAACGTTATGAAATGTTTAAATTTAAAATGTTTT 109 0.10296326384100206 No Hit GTCGGTGGGGCCTTTTTTTCCCTCAGCGCTGGCCGCGGCCTCCCGGCGCTCTCGTC 107 0.10107402964208458 No Hit GTCGGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC 107 0.10107402964208458 No Hit GTCGGTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGGGGTCCACGCCGAGCGAC 107 0.10107402964208458 No Hit GTCGGTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 106 0.10012941254262583 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0018892341989174687 0.0 0.0 0.0 0.0 11 0.0037784683978349374 0.0 0.0 0.0 0.0 12 0.006612319696211141 0.0 0.0 0.0 0.0 13 0.006612319696211141 0.0 0.0 0.0 0.0 14 0.011335405193504813 0.0 0.0 0.0 0.0 15 0.011335405193504813 0.0 0.0 0.0 0.0 16 0.012280022292963547 0.0 0.0 0.0 0.0 17 0.012280022292963547 0.0 0.0 0.0 0.0 18 0.013224639392422282 0.0 0.0 0.0 0.0 19 0.013224639392422282 0.0 0.0 0.0 0.0 20 0.01700310779025722 0.0 0.0 0.0 0.0 21 0.017947724889715952 0.0 0.0 0.0 0.0 22 0.017947724889715952 0.0 0.0 0.0 0.0 23 0.02172619328755089 0.0 0.0 0.0 0.0 24 0.022670810387009625 0.0 0.0 0.0 0.0 25 0.022670810387009625 0.0 0.0 0.0 0.0 26 0.022670810387009625 0.0 0.0 0.0 0.0 27 0.022670810387009625 0.0 0.0 0.0 0.0 28 0.03967391817726684 0.0 0.0 0.0 0.0 29 0.03967391817726684 0.0 0.0 0.0 0.0 30 0.04534162077401925 0.0 0.0 0.0 0.0 31 0.04723085497293672 0.0 0.0 0.0 0.0 32 0.04723085497293672 0.0 0.0 0.0 0.0 33 0.04912008917185419 0.0 0.0 0.0 0.0 34 0.050064706271312924 0.0 0.0 0.0 0.0 35 0.06140011146481773 0.0 0.0 0.0 0.0 36 0.06423396276319394 0.0 0.0 0.0 0.0 37 0.06423396276319394 0.0 0.0 0.0 0.0 38 0.06612319696211141 0.0 0.0 0.0 0.0 39 0.06612319696211141 0.0 0.0 0.0 0.0 40 0.06801243116102888 0.0 0.0 0.0 0.0 41 0.06801243116102888 0.0 0.0 0.0 0.0 42 0.06801243116102888 0.0 0.0 0.0 0.0 43 0.06801243116102888 0.0 0.0 0.0 0.0 44 0.06801243116102888 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCCCGT 15 0.008284968 50.18964 49 AGTTGTT 15 0.008284968 50.18964 50 ATGAGAG 15 0.008284968 50.18964 49 TAGAAAT 50 7.2759576E-12 50.189636 50 TTTTTAC 20 4.0893536E-4 50.189636 50 ACGACTC 20 4.0893536E-4 50.189636 50 TTGTGCA 25 2.028512E-5 50.189636 50 CTACTTT 20 4.0893536E-4 50.189636 48 CTATTAT 35 5.0744347E-8 50.189636 48 TATACTA 35 5.093534E-8 50.1658 44 TTATACT 35 5.093534E-8 50.1658 43 ATTATAC 35 5.093534E-8 50.1658 42 ATACTAT 35 5.093534E-8 50.1658 45 TACTATT 35 5.093534E-8 50.1658 46 ACTATTA 35 5.093534E-8 50.1658 47 CGCGACC 20 4.0989608E-4 50.165794 42 GTCACGG 20 4.0989608E-4 50.165794 42 GAAACCC 15 0.008300553 50.165794 46 AAATTAG 50 7.2759576E-12 50.165794 46 CCAAATC 15 0.008300553 50.165794 46 >>END_MODULE