SRR212548_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212548_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	100625
Sequences flagged as poor quality	0
Sequence length	56
%GC	58

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	2002	1.9895652173913043	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	1545	1.5354037267080745	No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	1436	1.4270807453416148	No Hit
GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	1324	1.3157763975155279	No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	1269	1.2611180124223602	No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC	1011	1.00472049689441	No Hit
GTCGGTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC	920	0.9142857142857144	No Hit
GTCGGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	919	0.9132919254658385	No Hit
GTCGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	641	0.6370186335403727	No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC	607	0.6032298136645963	No Hit
GTCGGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	602	0.5982608695652174	No Hit
GTCGGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC	582	0.5783850931677018	No Hit
GTCGGTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTC	566	0.5624844720496894	No Hit
GTCGGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	462	0.4591304347826087	No Hit
GTCGGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA	425	0.422360248447205	No Hit
GTCGGTGGGACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	406	0.4034782608695652	No Hit
GTCGGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	397	0.39453416149068327	No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	367	0.36472049689440994	No Hit
GTCGGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	330	0.32795031055900625	No Hit
GTCGGTGGGATTGCAGATATCTGATGGATGTAGTTTTCTTTTTTTAAATTAGAAAT	306	0.30409937888198757	No Hit
GTCGGTGGGAGTCTTAACATACATGTAGCAGATTGTATGTCATAGGGTACTTCTGT	303	0.30111801242236025	No Hit
GTCGGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	297	0.2951552795031056	No Hit
GTCGGTGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG	280	0.2782608695652174	No Hit
GTCGGTGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	274	0.2722981366459627	No Hit
GTCGGTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC	229	0.22757763975155282	No Hit
GTCGGTGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT	228	0.22658385093167702	No Hit
GTCGGTGGGGAGCCAGCCCCAGAGAGGTGAGAGGAAGTGGTGTCAAGGGCTTTGCC	224	0.2226086956521739	No Hit
GTCGGTGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG	224	0.2226086956521739	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT	215	0.21366459627329193	No Hit
GTCGGTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	213	0.21167701863354038	No Hit
GTCGGTGGGGCTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT	211	0.2096894409937888	No Hit
GTCGGTGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT	203	0.2017391304347826	No Hit
GTCGGTGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGGGGGCCCAAGAAACA	201	0.19975155279503107	No Hit
GTCGGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	200	0.1987577639751553	No Hit
GTCGGTGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	190	0.1888198757763975	No Hit
GTCGGTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	180	0.17888198757763973	No Hit
GTCGGTGGGATCACGTTCTCCTGTGGGCCAGTATTGTGGAACGAGTCTGAGTTGTT	180	0.17888198757763973	No Hit
GTCGGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	173	0.17192546583850932	No Hit
GTCGGTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGGCCACGCCGAGCGAC	169	0.1679503105590062	No Hit
GTCGGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	165	0.16397515527950313	No Hit
GTCGGTGGGACCATTTTTGTGGGTGTATATATATATTTGACTTGGGGAGAATTATT	151	0.15006211180124224	No Hit
GTCGGTGGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCTC	150	0.14906832298136646	No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGGGGCCTTCTCC	149	0.1480745341614907	No Hit
GTCGGTGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	144	0.1431055900621118	No Hit
GTCGGTGGGATCCAGCCAGTGGCCTGAACTTTGAAACCAGCAGCCCCAAATCCTGC	142	0.14111801242236025	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGCTCTTCC	139	0.13813664596273292	No Hit
GTCGGTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG	135	0.1341614906832298	No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTTAGTGGGTAAAAGCACCCGACTGCTCTTC	131	0.1301863354037267	No Hit
GTCGGTGGGGCCTTTTTTTCCCTCAGCGCTGGCCGCGGCCTCCCGGCGCTCTCGTC	131	0.1301863354037267	No Hit
GTCGGTGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG	127	0.1262111801242236	No Hit
GTCGGTGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	121	0.12024844720496894	No Hit
GTCGGTGGGGCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG	120	0.11925465838509317	No Hit
GTCGGTGGGAATTTGGAAGGGTGGAAATGATGTAAATATAGTATTCATGTATGAAA	119	0.11826086956521738	No Hit
GTCGGTGGGTTGAAAGCTCTGGTTGTTTCCTTTGTGAAAGCAGGCAGATACTTATT	117	0.11627329192546584	No Hit
GTCGGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	116	0.11527950310559006	No Hit
GTCGGTGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGGGGTCCACGCCGAGCGAC	116	0.11527950310559006	No Hit
GTCGGTGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	114	0.11329192546583851	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCA	113	0.11229813664596273	No Hit
GTCGGTGGGGAATATAGATTGTAGTGTGTGGTTCTCTTTTGAAATTTTTTTCAGGT	113	0.11229813664596273	No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCC	112	0.11130434782608695	No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTCTTC	109	0.10832298136645963	No Hit
GTCGGTGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGA	108	0.10732919254658387	No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCG	108	0.10732919254658387	No Hit
GTCGGTGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	107	0.10633540372670808	No Hit
GTCGGTGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACG	106	0.10534161490683229	No Hit
GTCGGTGGGAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGGGAT	106	0.10534161490683229	No Hit
GTCGGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	104	0.10335403726708076	No Hit
GTCGGTGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTCGGGG	103	0.10236024844720497	No Hit
GTCGGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC	103	0.10236024844720497	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTGAGC	20	4.032021E-4	50.330826	50
AATCTTC	20	4.032021E-4	50.330826	50
GTTTTAC	20	4.032021E-4	50.330826	49
TTATATG	20	4.032021E-4	50.330826	50
ACTTCTG	35	4.9598384E-8	50.330826	49
ACAAGCG	20	4.032021E-4	50.330826	50
GATACAA	25	1.9943107E-5	50.330822	50
GCGCGAT	15	0.008192218	50.330822	50
ATGCGCC	15	0.008192218	50.330822	50
AACCCCG	25	1.9943107E-5	50.330822	50
TTTTATG	15	0.008192218	50.330822	50
GAGTTGT	30	9.920204E-7	50.330822	49
TAGAAGG	25	2.0061989E-5	50.280342	48
TATCGGG	20	4.052054E-4	50.280342	48
CTATCGG	20	4.052054E-4	50.280342	47
GAGAATT	30	9.989199E-7	50.28034	47
TGCACCC	15	0.0082248	50.28034	48
ACATGCG	15	0.0082248	50.28034	48
CGAATGA	15	0.0082248	50.28034	47
CTGCACC	15	0.0082248	50.28034	47

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content