FastQCFastQC Report
Tue 31 May 2016
SRR212547_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212547_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64533
Sequences flagged as poor quality0
Sequence length58
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGACCCTTTCTCTAGAAGGTGCTAGTGGACATTTAAGATCTTCCTTGATAT23363.619853408333721No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT17432.7009437032216073No Hit
AGCGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC13142.0361675421877177No Hit
AGCGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG10311.597632219174686No Hit
AGCGAGGGGTACCTCACTCTTTACGTACGTGGTGACTGTTCGCACAGTCTGTGCTGCA6551.0149845815319294No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA6410.9932902545984226No Hit
AGCGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT5410.8383307765019447No Hit
AGCGAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5190.8042396913207196No Hit
AGCGAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5110.7918429330730014No Hit
AGCGAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3810.5903956115475802No Hit
AGCGAGGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAG3550.5501061472424961No Hit
AGCGAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3450.5346101994328483No Hit
AGCGAGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC3350.5191142516232006No Hit
AGCGAGGGGGTGGAAGTAATATCTAATCGCTCAAAGTCCAAAAAAAAAACAATAATGA2940.45558086560364464No Hit
AGCGAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2820.4369857282320673No Hit
AGCGAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC2700.41839059086048996No Hit
AGCGAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA2480.38429950567926485No Hit
AGCGAGGGGGTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGCTACCA2220.3440100413741807No Hit
AGCGAGGGGGGCGGCGCGGTCGACTACCGGGCGAAGGCCGCGGAGCAGCGTCACCTTG2210.34246044659321584No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA2000.30991895619295556No Hit
AGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1750.27117908666883606No Hit
AGCGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.2634311127640122No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1640.25413354407822353No Hit
AGCGAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1630.2525839492972588No Hit
AGCGAGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC1620.25103435451629397No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1580.2448359753924349No Hit
AGCGAGGGGGTTAGTGCTAGTGGTTCTGTTTTGTTTTTGTTTTTTGTTTTTTAAGGAA1510.23398881192568144No Hit
AGCGAGGGGGGGAGTTGGAGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTCTTCCA1390.21539367455410413No Hit
AGCGAGGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCG1380.21384407977313932No Hit
AGCGAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1350.20919529543024498No Hit
AGCGAGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG1290.19989772674445633No Hit
AGCGAGGGGGATCTTTGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAG1290.19989772674445633No Hit
AGCGAGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAGA1220.1890505632777029No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1190.18440177893480855No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG1180.18285218415384377No Hit
AGCGAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1170.181302589372879No Hit
AGCGAGGGGAACCAAAAGGATGTGGGGTTTTTCCTTGGGTTATGTCTCCTTATTCAAG1150.17820339981094943No Hit
AGCGAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGA1120.17355461546805512No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT1100.17045542590612556No Hit
AGCGAGGGGAGGCAAGAAATTGCGCCCTGAAAGACTGGCTGACTATGAGGCCCAAAAG1100.17045542590612556No Hit
AGCGAGGGGAGCAGGCCTGCACCACGGAGCTCGCCCTGCGGCTGCAGCAAGTGCAGAG1050.16270745200130166No Hit
AGCGAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA1020.15805866765840731No Hit
AGCGAGGGGGTCATTTTATTTCAAAGTAAAGATGGTGACAGTAGCCAGAAATCTTTTA1010.15650907287744253No Hit
AGCGAGGGGACCAAGAAACGCTATCAGATAAAGTGACTCCAGAAGATGCCTCTTCTCA1000.15495947809647778No Hit
AGCGAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1000.15495947809647778No Hit
AGCGAGGGGGGAGAAACCAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCTCTGCGGA1000.15495947809647778No Hit
AGCGAGGGGGGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGGGA990.153409883315513No Hit
AGCGAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT980.15186028853454822No Hit
AGCGAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC970.15031069375358344No Hit
AGCGAGGGGTATATTAAAAATGGACCTATTTTAATACTGTTCTGTCAGGAAAATGTTA930.14411231462972432No Hit
AGCGAGGGGGAGTGGGTTCTTGGTTTTAACCATGACCCACATTCTCACTTGAGTGTTG910.1410131250677948No Hit
AGCGAGGGGCGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAG910.1410131250677948No Hit
AGCGAGGGGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.13946353028682998No Hit
AGCGAGGGGACTGTCAGTTCCGCCCTCGGTGTCGTCGGGTCGCCAGCTTCCTGGGCAG860.13326515116297089No Hit
AGCGAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT860.13326515116297089No Hit
AGCGAGGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT840.13016596160104132No Hit
AGCGAGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC830.12861636682007654No Hit
AGCGAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA830.12861636682007654No Hit
AGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.12861636682007654No Hit
AGCGAGGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA800.12396758247718222No Hit
AGCGAGGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGGTC780.12086839291525266No Hit
AGCGAGGGGGGTAGATTATCCACCCTGCAGTCGGGTCGGCTGGGGATAGTCAAGGGGG780.12086839291525266No Hit
AGCGAGGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGT780.12086839291525266No Hit
AGCGAGGGGGCACAGGGCAAAGATGGCGGCGGAGGAGCAGTAGGCGTTGAGGGAGTTC770.11931879813428788No Hit
AGCGAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC750.11621960857235833No Hit
AGCGAGGGGCAGCTCCTGTCTCCCAACTATATAGTAATCATTAGTAGACTGTTGCCTT740.11467001379139355No Hit
AGCGAGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA740.11467001379139355No Hit
AGCGAGGGGGTTTGGAAAGGGGCCCTCAAGTTTTTGGCATTTTTCTTTCCTTTTTGTA740.11467001379139355No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGTTTTCTCCT730.11312041901042878No Hit
AGCGAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA730.11312041901042878No Hit
AGCGAGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTCTTTCTTCCGCCCC720.111570824229464No Hit
AGCGAGGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCT710.11002122944849921No Hit
AGCGAGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG710.11002122944849921No Hit
AGCGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.10382285032464011No Hit
AGCGAGGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC660.10227325554367533No Hit
AGCGAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA660.10227325554367533No Hit
AGCGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG650.10072366076271054No Hit
AGCGAGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG650.10072366076271054No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCAC150.00716863152.03804450
CGCGAAG203.410921E-452.03804442
CGTCACC150.00716863152.03804449
TGGCATT150.00716863152.03804435
ACCGGAG150.00716863152.03804439
CAATGCG251.6305927E-552.03804452
ACTAACA150.00716863152.03804430
ATCTTTT203.410921E-452.03804451
TTGCAAT251.6305927E-552.03804449
CATTGGC203.410921E-452.03804447
AACAACC150.00716863152.03804433
CACATTC150.00716863152.03804438
GTCATGG150.00716863152.03804440
TAAACGC150.00716863152.03804447
TTCGCTA1500.052.03804433
GCCCAAA203.410921E-452.03804450
CCAGCTT150.00716863152.03804443
AAATTCG150.00716863152.03804448
AGAAGAT150.00716863152.03804441
TTCGTAT150.00716863152.03804451
Produced by FastQC (version 0.11.5)