FastQCFastQC Report
Tue 31 May 2016
SRR212546_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212546_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64543
Sequences flagged as poor quality0
Sequence length58
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGACCCTTTCTCTAGAAGGTGCTAGTGGACATTTAAGATCTTCCTTGATAT21323.303224207117735No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT16362.534744279007793No Hit
AGCGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC14012.170645925971833No Hit
AGCGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG10341.6020327533582264No Hit
AGCGAGGGGTACCTCACTCTTTACGTACGTGGTGACTGTTCGCACAGTCTGTGCTGCA6190.9590505554436577No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA5910.9156686240180965No Hit
AGCGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT5340.8273554064732039No Hit
AGCGAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5040.7808747656601026No Hit
AGCGAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4560.7065057403591404No Hit
AGCGAGGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAG3510.5438234975132857No Hit
AGCGAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3480.5391754334319756No Hit
AGCGAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3470.5376260787382056No Hit
AGCGAGGGGGTGGAAGTAATATCTAATCGCTCAAAGTCCAAAAAAAAAACAATAATGA3380.523681886494275No Hit
AGCGAGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC2710.4198751220116821No Hit
AGCGAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC2680.415227057930372No Hit
AGCGAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2650.4105789938490619No Hit
AGCGAGGGGGTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGCTACCA2460.381141254667431No Hit
AGCGAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA2460.381141254667431No Hit
AGCGAGGGGGGCGGCGCGGTCGACTACCGGGCGAAGGCCGCGGAGCAGCGTCACCTTG1870.2897293277349984No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1860.28817997304122833No Hit
AGCGAGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC1750.27113707140975785No Hit
AGCGAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1660.25719287916582745No Hit
AGCGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.2463473963094371No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1590.2463473963094371No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1570.24324868692189705No Hit
AGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1560.24169933222812698No Hit
AGCGAGGGGGTTAGTGCTAGTGGTTCTGTTTTGTTTTTGTTTTTTGTTTTTTAAGGAA1540.2386006228405869No Hit
AGCGAGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG1380.21381094774026616No Hit
AGCGAGGGGGGGAGTTGGAGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTCTTCCA1370.2122615930464961No Hit
AGCGAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1270.19676804610879567No Hit
AGCGAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1170.18127449917109523No Hit
AGCGAGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAGA1160.1797251444773252No Hit
AGCGAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1160.1797251444773252No Hit
AGCGAGGGGGATCTTTGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAG1130.17507708039601505No Hit
AGCGAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGA1110.171978371008475No Hit
AGCGAGGGGTATATTAAAAATGGACCTATTTTAATACTGTTCTGTCAGGAAAATGTTA1100.17042901631470492No Hit
AGCGAGGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCG1090.16887966162093487No Hit
AGCGAGGGGGTCATTTTATTTCAAAGTAAAGATGGTGACAGTAGCCAGAAATCTTTTA1090.16887966162093487No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1060.16423159753962474No Hit
AGCGAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCCCC1050.1626822428458547No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT1040.16113288815208465No Hit
AGCGAGGGGGGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGGGA1030.1595835334583146No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG1020.15803417876454456No Hit
AGCGAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT980.1518367599894644No Hit
AGCGAGGGGACCAAGAAACGCTATCAGATAAAGTGACTCCAGAAGATGCCTCTTCTCA960.1487380506019243No Hit
AGCGAGGGGAGGCAAGAAATTGCGCCCTGAAAGACTGGCTGACTATGAGGCCCAAAAG940.1456393412143842No Hit
AGCGAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT930.14408998652061417No Hit
AGCGAGGGGAGCAGGCCTGCACCACGGAGCTCGCCCTGCGGCTGCAGCAAGTGCAGAG930.14408998652061417No Hit
AGCGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.13634321305176395No Hit
AGCGAGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC870.1347938583579939No Hit
AGCGAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA870.1347938583579939No Hit
AGCGAGGGGAACCAAAAGGATGTGGGGTTTTTCCTTGGGTTATGTCTCCTTATTCAAG860.13324450366422386No Hit
AGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.12859643958291372No Hit
AGCGAGGGGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.12394837550160358No Hit
AGCGAGGGGGGAGAAACCAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCTCTGCGGA790.12239902080783352No Hit
AGCGAGGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT790.12239902080783352No Hit
AGCGAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC780.12084966611406349No Hit
AGCGAGGGGCAGCTCCTGTCTCCCAACTATATAGTAATCATTAGTAGACTGTTGCCTT770.11930031142029345No Hit
AGCGAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA770.11930031142029345No Hit
AGCGAGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG760.11775095672652341No Hit
AGCGAGGGGGAGTGGGTTCTTGGTTTTAACCATGACCCACATTCTCACTTGAGTGTTG740.1146522473389833No Hit
AGCGAGGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA740.1146522473389833No Hit
AGCGAGGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGT740.1146522473389833No Hit
AGCGAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC730.11310289264521327No Hit
AGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA730.11310289264521327No Hit
AGCGAGGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGGTC720.11155353795144322No Hit
AGCGAGGGGCGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAG720.11155353795144322No Hit
AGCGAGGGGGTTTGGAAAGGGGCCCTCAAGTTTTTGGCATTTTTCTTTCCTTTTTGTA720.11155353795144322No Hit
AGCGAGGGGGGCCGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTT720.11155353795144322No Hit
AGCGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT710.11000418325767317No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA710.11000418325767317No Hit
AGCGAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA700.10845482856390314No Hit
AGCGAGGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTCCT680.10535611917636305No Hit
AGCGAGGGGGGACCCGCGGTGGTGGCGCACGCCTTTAATCCTTTGCCTTCTGAGTGCT680.10535611917636305No Hit
AGCGAGGGGGGTAGATTATCCACCCTGCAGTCGGGTCGGCTGGGGATAGTCAAGGGGG670.103806764482593No Hit
AGCGAGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCCCC650.10070805509505291No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGTTTTCTCCT650.10070805509505291No Hit
AGCGAGGGGACTGTCAGTTCCGCCCTCGGTGTCGTCGGGTCGCCAGCTTCCTGGGCAG650.10070805509505291No Hit
AGCGAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA650.10070805509505291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGATA2800.052.05517251
AATCTTC203.4053662E-452.0551750
AAATCTT401.8299033E-952.0551749
TATATAA203.4053662E-452.0551752
CTCCCGA203.4053662E-452.0551752
CCTGCAG203.4053662E-452.0551724
TTCCGAA203.4053662E-452.0551752
GTCAAGG203.4053662E-452.0551749
TTATCTC203.4053662E-452.0551747
TATGTAC203.4053662E-452.0551727
GGCCGCT458.913048E-1152.0551750
AATTATG203.4053662E-452.0551724
TTGAGGG203.4053662E-452.0551747
AGGGAGT203.4053662E-452.0551750
ATTATGT203.4053662E-452.0551725
CACCTTG203.4053662E-452.0551752
CGGCGGA203.4053662E-452.0551727
TACGTGG550.052.0551726
TACCGGG203.4053662E-452.0551725
AGGCCCA203.4053662E-452.0551748
Produced by FastQC (version 0.11.5)