FastQCFastQC Report
Tue 31 May 2016
SRR212545_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212545_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63734
Sequences flagged as poor quality0
Sequence length58
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGACCCTTTCTCTAGAAGGTGCTAGTGGACATTTAAGATCTTCCTTGATAT20123.156870744029874No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT15142.3754981642451436No Hit
AGCGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC12531.965983619418207No Hit
AGCGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG8901.3964289076474095No Hit
AGCGAGGGGTACCTCACTCTTTACGTACGTGGTGACTGTTCGCACAGTCTGTGCTGCA6170.9680861078859009No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA5470.8582546207675653No Hit
AGCGAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC5120.8033388772083974No Hit
AGCGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT4580.7186117300028242No Hit
AGCGAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC4340.6809552201336806No Hit
AGCGAGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC3710.5821068817271786No Hit
AGCGAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3490.5475884143471302No Hit
AGCGAGGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAG3480.5460193931025826No Hit
AGCGAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT3280.5146389682116296No Hit
AGCGAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC2560.4016694386041987No Hit
AGCGAGGGGGTGGAAGTAATATCTAATCGCTCAAAGTCCAAAAAAAAAACAATAATGA2520.39539335362600814No Hit
AGCGAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2290.3593058650014121No Hit
AGCGAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA2020.31694229139862556No Hit
AGCGAGGGGGGCGGCGCGGTCGACTACCGGGCGAAGGCCGCGGAGCAGCGTCACCTTG1820.2855618665076725No Hit
AGCGAGGGGGTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGCTACCA1730.27144067530674365No Hit
AGCGAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1730.27144067530674365No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1590.24947437788307653No Hit
AGCGAGGGGGGGAGTTGGAGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTCTTCCA1470.23064612294850473No Hit
AGCGAGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC1430.22437003797031413No Hit
AGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1320.20711080428028997No Hit
AGCGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.20083471930209934No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1260.19769667681300407No Hit
AGCGAGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG1170.1835754856120752No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1150.18043744312297988No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA1100.17259233690024164No Hit
AGCGAGGGGGTTAGTGCTAGTGGTTCTGTTTTGTTTTTGTTTTTTGTTTTTTAAGGAA1080.16945429441114634No Hit
AGCGAGGGGGTCATTTTATTTCAAAGTAAAGATGGTGACAGTAGCCAGAAATCTTTTA1020.16004016694386042No Hit
AGCGAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1000.15690212445476512No Hit
AGCGAGGGGAACCAAAAGGATGTGGGGTTTTTCCTTGGGTTATGTCTCCTTATTCAAG980.15376408196566982No Hit
AGCGAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA970.15219506072112216No Hit
AGCGAGGGGTATATTAAAAATGGACCTATTTTAATACTGTTCTGTCAGGAAAATGTTA950.14905701823202686No Hit
AGCGAGGGGGGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGGGA940.14748799698747922No Hit
AGCGAGGGGAGCAGGCCTGCACCACGGAGCTCGCCCTGCGGCTGCAGCAAGTGCAGAG940.14748799698747922No Hit
AGCGAGGGGGATCTTTGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAG940.14748799698747922No Hit
AGCGAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGA930.14591897574293156No Hit
AGCGAGGGGAGGCAAGAAATTGCGCCCTGAAAGACTGGCTGACTATGAGGCCCAAAAG920.14434995449838392No Hit
AGCGAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC890.13964289076474096No Hit
AGCGAGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAGA890.13964289076474096No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG870.13650484827564566No Hit
AGCGAGGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCG860.134935827031098No Hit
AGCGAGGGGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.134935827031098No Hit
AGCGAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA810.12709072080835973No Hit
AGCGAGGGGACCAAGAAACGCTATCAGATAAAGTGACTCCAGAAGATGCCTCTTCTCA800.1255216995638121No Hit
AGCGAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT790.12395267831926443No Hit
AGCGAGGGGGGAGAAACCAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCTCTGCGGA790.12395267831926443No Hit
AGCGAGGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA790.12395267831926443No Hit
AGCGAGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC780.1223836570747168No Hit
AGCGAGGGGGAGTGGGTTCTTGGTTTTAACCATGACCCACATTCTCACTTGAGTGTTG780.1223836570747168No Hit
AGCGAGGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT780.1223836570747168No Hit
AGCGAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC770.12081463583016915No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGTTTTCTCCT770.12081463583016915No Hit
AGCGAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA750.11767659334107385No Hit
AGCGAGGGGCAGCTCCTGTCTCCCAACTATATAGTAATCATTAGTAGACTGTTGCCTT720.11296952960743087No Hit
AGCGAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA720.11296952960743087No Hit
AGCGAGGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGGTC710.11140050836288323No Hit
AGCGAGGGGCGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAG710.11140050836288323No Hit
AGCGAGGGGGGTAGATTATCCACCCTGCAGTCGGGTCGGCTGGGGATAGTCAAGGGGG700.1098314871183356No Hit
AGCGAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGT700.1098314871183356No Hit
AGCGAGGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA690.10826246587378793No Hit
AGCGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.10512442338469262No Hit
AGCGAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT670.10512442338469262No Hit
AGCGAGGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGT670.10512442338469262No Hit
AGCGAGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG660.10355540214014498No Hit
AGCGAGGGGGGCCGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTT660.10355540214014498No Hit
AGCGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT640.10041735965104967No Hit
AGCGAGGGGGCACAGGGCAAAGATGGCGGCGGAGGAGCAGTAGGCGTTGAGGGAGTTC640.10041735965104967No Hit
AGCGAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA640.10041735965104967No Hit
AGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.10041735965104967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTA1400.052.435725
GGATGTT150.00695534852.4357158
GGATGTA150.00695534852.4357158
GGATGGA203.284563E-452.4357158
GTCATTT203.284563E-452.43571510
GGACTTT150.00695534852.4357158
GGCGTGA150.00695534852.4357159
GGGTACC750.052.4357157
GGTTAGT150.00695534852.4357159
AGTGTAA203.284563E-452.43571510
GGGACGT251.5583626E-552.4357158
GGGATGT401.715307E-952.4357157
GGGACAG203.284563E-452.4357157
GGGATAT150.00695534852.4357157
GGGGCGA251.5583626E-552.4357156
GGTGGAA401.715307E-952.4357159
GGGGTAC900.052.4357156
GCTTCTT203.284563E-452.43571510
GGGTGAT150.00695534852.4357157
GGTACAT150.00695534852.4357158
Produced by FastQC (version 0.11.5)