##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212545_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 63734 Sequences flagged as poor quality 0 Sequence length 58 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.79246555998368 39.0 38.0 40.0 34.0 40.0 2 37.2768067279631 39.0 37.0 40.0 33.0 40.0 3 36.87578058806916 39.0 36.0 39.0 31.0 40.0 4 37.32139831173314 39.0 37.0 40.0 33.0 40.0 5 36.18100229077102 38.0 35.0 39.0 30.0 40.0 6 37.540873003420465 39.0 38.0 40.0 33.0 40.0 7 37.91751655317413 39.0 38.0 40.0 35.0 40.0 8 37.87702011485236 39.0 38.0 40.0 35.0 40.0 9 37.04123387830671 39.0 35.0 40.0 31.0 40.0 10 35.707503059591424 39.0 35.0 40.0 29.0 40.0 11 36.40574889384002 39.0 35.0 40.0 31.0 40.0 12 36.75069821445383 39.0 35.0 40.0 31.0 40.0 13 36.67502431982929 39.0 35.0 40.0 31.0 40.0 14 36.62490978127844 39.0 36.0 40.0 31.0 40.0 15 37.0189067059968 39.0 36.0 40.0 33.0 40.0 16 36.90432108450749 38.0 36.0 40.0 33.0 40.0 17 36.32477170740892 38.0 35.0 40.0 31.0 40.0 18 36.4649794458217 38.0 35.0 40.0 31.0 40.0 19 36.32822355414692 38.0 35.0 39.0 31.0 40.0 20 36.212602378636205 38.0 35.0 39.0 30.0 40.0 21 36.187545109360784 38.0 35.0 39.0 30.0 40.0 22 36.03665233627263 38.0 35.0 39.0 30.0 40.0 23 35.80978755452349 38.0 35.0 39.0 29.0 40.0 24 35.767282769008695 38.0 35.0 39.0 29.0 40.0 25 35.725029026893026 38.0 35.0 39.0 29.0 40.0 26 35.52215457997301 38.0 35.0 39.0 29.0 40.0 27 35.080663382182195 38.0 34.0 39.0 28.0 40.0 28 35.08872815137917 38.0 34.0 39.0 28.0 40.0 29 34.84576521166097 38.0 33.0 39.0 27.0 40.0 30 34.77757554837292 38.0 33.0 39.0 27.0 40.0 31 34.344243261053755 38.0 33.0 39.0 26.0 40.0 32 34.06905262497254 38.0 33.0 39.0 25.0 40.0 33 33.97908494681018 37.0 33.0 39.0 25.0 40.0 34 33.658220102300184 37.0 33.0 39.0 23.0 40.0 35 32.86815514482066 36.0 31.0 39.0 20.0 40.0 36 33.795776194809676 38.0 33.0 39.0 24.0 40.0 37 33.4200583675903 37.0 33.0 39.0 23.0 40.0 38 33.10432422254998 37.0 32.0 39.0 20.0 40.0 39 32.71696425769605 37.0 32.0 39.0 17.0 40.0 40 32.88425330278972 37.0 32.0 39.0 20.0 40.0 41 33.06078388301378 38.0 33.0 39.0 19.0 40.0 42 32.82461480528446 37.0 32.0 39.0 18.0 40.0 43 32.376784761665675 37.0 31.0 39.0 16.0 40.0 44 32.259202309599274 37.0 31.0 39.0 12.0 40.0 45 32.077603790755326 36.0 31.0 39.0 11.0 40.0 46 31.47439357328898 36.0 30.0 39.0 4.0 40.0 47 31.34207801173628 36.0 30.0 39.0 4.0 40.0 48 31.63620673423918 36.0 30.0 39.0 2.0 40.0 49 31.42562839300844 36.0 30.0 39.0 2.0 40.0 50 30.920937019487244 36.0 29.0 39.0 2.0 40.0 51 31.110772899865065 36.0 30.0 39.0 2.0 40.0 52 30.757397935168044 36.0 30.0 39.0 2.0 40.0 53 30.639705651614523 36.0 30.0 39.0 2.0 40.0 54 30.39365174004456 36.0 29.0 39.0 2.0 40.0 55 30.14034895032479 36.0 29.0 39.0 2.0 40.0 56 29.774735619920293 36.0 29.0 39.0 2.0 40.0 57 29.57273982489723 36.0 29.0 39.0 2.0 40.0 58 29.1760284934258 35.0 28.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.1883473395295496 1 2 -2.768745120317213 1 3 -3.0103572581262554 1 4 -2.5093346289555143 1 5 -4.922177198814577 1 6 -2.3207565823834173 1 7 -3.7591865525551285 1 8 -3.9403320141562332 1 9 -4.442751732136358 1 10 -4.7451577962611715 1 11 -3.6393160911844546 1 12 -2.778816741620588 1 13 -2.9799839744038508 1 14 -2.4882557976937676 1 15 -2.4858573772123123 1 16 -2.65682943089665 1 17 -3.7540090724371566 1 18 -4.431745940736967 1 19 -5.297131616480716 1 20 -4.316424041490759 1 21 -2.885583702907489 1 22 -2.792025070091988 1 23 -2.723573701374214 1 24 -3.2832763849057756 1 25 -1.6955459258251935 1 26 -3.039183046472253 1 27 -2.8169210886151745 1 28 -1.3392719458064661 1 29 -2.042917019445717 1 30 -2.9608223161739033 1 31 -2.5877503365174626 1 32 -1.7855797014138943 1 33 -3.0060122822448925 1 34 -2.4793115045987086 1 35 -2.251669769133315 1 36 -0.243412721067088 1 37 -0.08870218477443359 1 38 -1.6033656525030224 1 39 -0.9472932861390255 1 40 -0.44532688429418954 1 41 -1.9305097033611638 1 42 -4.617012512400198 1 43 -1.639340620634652 1 44 -2.9332661040358943 1 45 -2.502732376405902 1 46 -1.2520572721446293 1 47 0.6156382493689456 1 48 -1.9693629764234117 1 49 0.12029559229289788 1 50 -3.5186540625980847 1 51 -0.955192531416877 1 52 -0.4546647486473354 1 53 -1.0007669762267 1 54 -1.3146767666273433 1 55 0.06938468273691711 1 56 1.0203919490581264 1 57 -0.03784065928460478 1 58 -0.42050990230091045 2 1 0.4486077678224376 2 2 0.0061410215175286 2 3 0.27201099171108467 2 4 0.02742529297031382 2 5 0.18192169513467604 2 6 0.08783157636739958 2 7 0.05864038303367636 2 8 -0.9188616172792052 2 9 -0.27163917520727665 2 10 0.32966328376485166 2 11 0.2682961404355879 2 12 0.4345859909753784 2 13 0.49952155584988844 2 14 0.5338652172704457 2 15 0.10034951933941016 2 16 0.11089991197908233 2 17 0.763232306873185 2 18 0.11542387058378978 2 19 0.6176374140983327 2 20 0.5098609292314293 2 21 -0.07035923966741109 2 22 -0.11538225942184965 2 23 1.0532642947220765 2 24 -0.6215977902408483 2 25 -0.579085288219467 2 26 0.09094057096431385 2 27 0.1837295294720107 2 28 -0.010059193691958512 2 29 0.5348318419726326 2 30 -0.012221145061346306 2 31 0.46494972854434735 2 32 0.25931294660172455 2 33 0.7200775030511366 2 34 0.2916709287129393 2 35 0.40740635318288554 2 36 1.5822216315679185 2 37 1.1956179193244552 2 38 1.134435258362302 2 39 1.149648808851218 2 40 2.635349758516483 2 41 2.5197180129693493 2 42 2.122740252726672 2 43 2.1303405765026326 2 44 2.6620494457363932 2 45 1.7223814817593563 2 46 0.9027898326048813 2 47 1.4490800190501396 2 48 1.43857456424022 2 49 0.7605037900807972 2 50 0.3160889432574798 2 51 1.8912616000079758 2 52 2.6090958238965847 2 53 1.8723624967726522 2 54 2.0848027389029085 2 55 1.9412129195619023 2 56 2.2461564253105664 2 57 1.9354839991408994 2 58 1.7987483930796984 3 1 -0.6823791697682751 3 2 -0.35561514684241047 3 3 -1.1688453217867334 3 4 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0.7905823946055364 119 35 0.12366179585342252 119 36 1.4356323717711135 119 37 0.7090431733157487 119 38 0.8720985384784115 119 39 1.090505122349036 119 40 1.245654548066554 119 41 1.2816918881507746 119 42 1.891608177254973 119 43 1.0828079204794108 119 44 1.0448506068394394 119 45 2.6995222655039157 119 46 2.0834864944336218 119 47 2.3696170292097953 119 48 2.018037554080564 119 49 1.764857926510409 119 50 2.533070075332951 119 51 2.199316752402755 119 52 1.6733193362333019 119 53 3.0171375330571166 119 54 2.0162250901365795 119 55 3.2126207570074747 119 56 3.150365423859185 119 57 3.2718410383281302 119 58 3.586121397433839 120 1 -0.425256662645765 120 2 -1.775130816358086 120 3 -1.0627199656613797 120 4 -1.8656564680359722 120 5 -2.1552551043062707 120 6 -1.3443836577346318 120 7 -1.717040958685395 120 8 -1.1382885914231622 120 9 -1.060248539288338 120 10 0.09136838509259348 120 11 0.2770312906801706 120 12 -0.23347829973042167 120 13 -0.6849648173157306 120 14 -0.43014596372186276 120 15 -0.5848356658457732 120 16 -1.1651308357497854 120 17 -1.3849158412749603 120 18 -2.2465607555517835 120 19 -1.7862759332112432 120 20 -1.4268965829977844 120 21 -1.27511116140046 120 22 -1.1311055298620971 120 23 -1.2395379414763852 120 24 -0.641513932798496 120 25 -0.9152138696310672 120 26 -1.1592341320405808 120 27 -0.9682620847837526 120 28 -0.32522341451655734 120 29 -0.3040919875172392 120 30 0.3284497144774363 120 31 -1.7908154706170762 120 32 0.4200397111020706 120 33 -0.7173788211976415 120 34 -0.633206779183638 120 35 -0.31935814716651834 120 36 0.5937520298907657 120 37 0.18268989696247218 120 38 0.40557432195419096 120 39 0.5214168032607205 120 40 0.26987107228308105 120 41 0.008187614646502084 120 42 0.7712378068845993 120 43 0.9168535045249939 120 44 1.2008335128223422 120 45 0.5036533196349708 120 46 1.8769337878809154 120 47 1.7107851203778814 120 48 1.4731657592087686 120 49 2.256310917963397 120 50 2.4589960012588747 120 51 -0.2757544726684742 120 52 1.6953991083130724 120 53 0.6218383877579754 120 54 1.3915812154927067 120 55 2.0502275946143094 120 56 3.116177389671151 120 57 2.500473516960607 120 58 3.096092907405346 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 87.0 3 12.0 4 20.0 5 11.0 6 17.0 7 43.0 8 54.0 9 75.0 10 99.0 11 111.0 12 145.0 13 146.0 14 218.0 15 208.0 16 274.0 17 272.0 18 305.0 19 336.0 20 406.0 21 415.0 22 558.0 23 672.0 24 890.0 25 830.0 26 897.0 27 894.0 28 1190.0 29 1387.0 30 1728.0 31 2172.0 32 2725.0 33 3335.0 34 4023.0 35 5359.0 36 6884.0 37 9188.0 38 10037.0 39 7706.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 58.67445468072309 26.643872479184477 4.908664957102606 9.773007882989837 11 37.28193452147288 19.364057470361935 15.372536704090445 27.981471304074745 12 37.01321115887909 12.385853704459159 23.94012614930806 26.66080898735369 13 19.808893212414098 19.83399755232686 31.03994728088618 29.31716195437286 14 16.46530894028305 23.196410079392475 41.45981736592714 18.87846361439734 15 20.29999686195751 13.550067467913514 46.213951736906516 19.935983933222456 16 23.293689396554427 14.824112718486207 40.34110521856466 21.541092666394704 17 24.02599441182934 13.909207923900418 19.852133237057735 42.21266442721251 18 26.036338532023724 16.589261618602315 26.709448645934664 30.664951203439294 19 24.69639438918003 19.00398531396115 31.204694511563684 25.094925785295132 20 30.464116484137193 18.44384472965764 32.27633602158973 18.815702764615434 21 28.026297208667412 20.595296000502096 28.112594731143993 23.265812059686507 22 36.986301369863014 18.042021685574856 23.414771924869367 21.556905019692763 23 34.95151724354348 25.833934791477077 21.748203470674994 17.466344494304455 24 30.608466438635578 23.604355602974863 21.580318197508394 24.206859760881162 25 29.706403879046555 25.080421171559937 22.393961743060242 22.819213206333266 26 32.06241072409625 20.08727455381669 23.8215580705417 24.028756651545354 27 21.988698791398527 16.02103280489719 29.359598179249723 32.63067022445456 28 26.585358196772773 27.373328310416273 21.91875431656935 24.122559176241605 29 29.39374420687163 17.576547845349 28.168350274142618 24.86135767363675 30 21.67750856352723 16.811225291474184 36.02180949687314 25.489456648125454 31 27.092127355751877 19.90835909426146 30.795424231487438 22.204089318499225 32 36.75051391090119 14.904200731244213 30.21953018343873 18.12575517441587 33 35.879851226440266 12.90626324131762 31.39153497277193 19.822350559470188 34 30.381832734349253 15.27017059275883 36.952871200112995 17.39512547277892 35 25.516754296476496 21.076669544063407 38.46817860786314 14.938397551596955 36 27.34994426389085 29.00004710093731 27.06262854630807 16.587380088863767 37 29.332621630532053 18.00869742688039 30.16939573292306 22.489285209664505 38 22.50847776940467 26.15234865611655 31.381876412961567 19.957297161517207 39 28.01389456477319 20.833988243060578 30.844676369809186 20.307440822357044 40 24.08465741380393 23.743955284808138 33.76405200025121 18.407335301136722 41 25.753317107639162 17.563005417288217 34.85436130957054 21.82931616550208 42 29.181388483221056 22.96760415194485 28.66789152180399 19.1831158430301 43 21.434067746038725 22.569449897140345 31.545721509445812 24.450760847375115 44 27.48474554947474 17.751777064855006 32.44637352959678 22.317103856073473 45 28.28466866665619 19.774765577143576 28.235977822105646 23.704587934094587 46 24.53781512605042 21.553443807429513 32.79195790465719 21.116783161862877 47 22.603299293008643 18.757266300078555 33.67321288295365 24.96622152395915 48 25.51369863013699 17.693540278999624 36.91875078547191 19.87401030539148 49 24.874707388729163 19.049190114845015 34.92168229878557 21.154420197640256 50 17.71266986096929 18.119550703008404 33.656429188594764 30.511350247427536 51 23.344438507716 14.932268912845334 34.42184995442688 27.301442625011784 52 17.89374410562716 20.12103112228859 40.144608613643506 21.840616158440742 53 16.71040020112188 19.302986974215546 43.07307952170702 20.913533302955546 54 22.12392161792668 20.04651382057608 33.11595455474017 24.713610006757076 55 21.38669265471007 21.308099781511814 32.81881199012874 24.486395573649382 56 20.94431945121855 20.245755911829953 31.80352113784043 27.006403499111066 57 21.721015062419422 23.403352095846042 29.866985314927202 25.00864752680733 58 21.934073538200234 23.489447362626994 32.0432170603592 22.533262038813575 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 17.0 12 34.0 13 45.0 14 56.0 15 72.5 16 89.5 17 90.0 18 193.5 19 297.0 20 422.5 21 548.0 22 492.5 23 463.0 24 489.0 25 621.0 26 753.0 27 890.5 28 1147.0 29 1266.0 30 1319.0 31 1372.0 32 1426.0 33 1480.0 34 1238.0 35 1373.5 36 1751.0 37 1334.5 38 918.0 39 752.0 40 586.0 41 546.0 42 640.0 43 774.0 44 1820.0 45 2866.0 46 2186.0 47 2004.5 48 2503.0 49 2013.0 50 1523.0 51 1546.0 52 1569.0 53 1847.5 54 2921.5 55 3717.0 56 3241.5 57 2766.0 58 2932.0 59 3034.0 60 2970.0 61 3962.5 62 4955.0 63 4988.5 64 5022.0 65 5246.0 66 4558.5 67 3647.0 68 3094.0 69 2541.0 70 1940.0 71 1339.0 72 1134.5 73 788.5 74 647.0 75 595.0 76 543.0 77 395.5 78 193.5 79 139.0 80 102.0 81 65.0 82 51.5 83 38.0 84 26.5 85 12.0 86 9.0 87 7.0 88 5.0 89 3.5 90 2.0 91 3.5 92 2.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.8786518969466848 11 0.07688204098283491 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.04393259484733423 18 0.0 19 0.0 20 0.0 21 0.001569021244547651 22 0.007845106222738256 23 0.0 24 0.0 25 0.010983148711833557 26 0.042363573602786586 27 0.03765650986914363 28 0.04079455235823893 29 0.12709072080835973 30 0.14434995449838392 31 0.010983148711833557 32 0.010983148711833557 33 0.020397276179119465 34 0.023535318668214768 35 0.02981140364640537 36 0.0643298710264537 37 0.05805378604826309 38 0.05962280729281074 39 0.17573037938933692 40 0.06589889227100135 41 0.07688204098283491 42 0.08315812596102551 43 0.08629616845012082 44 0.22907710170395706 45 0.10512442338469262 46 0.10826246587378793 47 0.1317977845420027 48 0.1223836570747168 49 0.13022876329745503 50 0.12395267831926443 51 0.15690212445476512 52 0.17886842187843222 53 0.14278093325383626 54 0.15219506072112216 55 0.18043744312297988 56 0.274578717795839 57 0.20711080428028997 58 0.23221514419305236 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 63734.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.63300593090031 #Duplication Level Percentage of deduplicated Percentage of total 1 75.18234947673629 26.03790755326827 2 8.852444162551533 6.1317350236922215 3 4.086440447605672 4.245771487745944 4 2.6004621030217914 3.6024727774814074 5 1.6264214198341866 2.8163931339630337 6 1.395369908938522 2.8995512599240594 7 1.032936166357088 2.5041579062980515 8 0.706745798033797 1.9581385131954687 9 0.5663027227834911 1.7651489001161074 >10 3.511076881257645 21.235133523707912 >50 0.2990078376296833 7.211221639941005 >100 0.1087301227744303 8.067907239464024 >500 0.018121687129071716 4.026108513509273 >1k 0.013591265346803787 7.498352527693225 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGCGAGGGGACCCTTTCTCTAGAAGGTGCTAGTGGACATTTAAGATCTTCCTTGATAT 2012 3.156870744029874 No Hit AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 1514 2.3754981642451436 No Hit AGCGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 1253 1.965983619418207 No Hit AGCGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 890 1.3964289076474095 No Hit AGCGAGGGGTACCTCACTCTTTACGTACGTGGTGACTGTTCGCACAGTCTGTGCTGCA 617 0.9680861078859009 No Hit AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 547 0.8582546207675653 No Hit AGCGAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 512 0.8033388772083974 No Hit AGCGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 458 0.7186117300028242 No Hit AGCGAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC 434 0.6809552201336806 No Hit AGCGAGGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCC 371 0.5821068817271786 No Hit AGCGAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 349 0.5475884143471302 No Hit AGCGAGGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAG 348 0.5460193931025826 No Hit AGCGAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 328 0.5146389682116296 No Hit AGCGAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCC 256 0.4016694386041987 No Hit AGCGAGGGGGTGGAAGTAATATCTAATCGCTCAAAGTCCAAAAAAAAAACAATAATGA 252 0.39539335362600814 No Hit AGCGAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG 229 0.3593058650014121 No Hit AGCGAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCTGA 202 0.31694229139862556 No Hit AGCGAGGGGGGCGGCGCGGTCGACTACCGGGCGAAGGCCGCGGAGCAGCGTCACCTTG 182 0.2855618665076725 No Hit AGCGAGGGGGTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGCTACCA 173 0.27144067530674365 No Hit AGCGAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA 173 0.27144067530674365 No Hit AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA 159 0.24947437788307653 No Hit AGCGAGGGGGGGAGTTGGAGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTCTTCCA 147 0.23064612294850473 No Hit AGCGAGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC 143 0.22437003797031413 No Hit AGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 132 0.20711080428028997 No Hit AGCGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 128 0.20083471930209934 No Hit AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT 126 0.19769667681300407 No Hit AGCGAGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCG 117 0.1835754856120752 No Hit AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 115 0.18043744312297988 No Hit AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 110 0.17259233690024164 No Hit AGCGAGGGGGTTAGTGCTAGTGGTTCTGTTTTGTTTTTGTTTTTTGTTTTTTAAGGAA 108 0.16945429441114634 No Hit AGCGAGGGGGTCATTTTATTTCAAAGTAAAGATGGTGACAGTAGCCAGAAATCTTTTA 102 0.16004016694386042 No Hit AGCGAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG 100 0.15690212445476512 No Hit AGCGAGGGGAACCAAAAGGATGTGGGGTTTTTCCTTGGGTTATGTCTCCTTATTCAAG 98 0.15376408196566982 No Hit AGCGAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA 97 0.15219506072112216 No Hit AGCGAGGGGTATATTAAAAATGGACCTATTTTAATACTGTTCTGTCAGGAAAATGTTA 95 0.14905701823202686 No Hit AGCGAGGGGGGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGGGA 94 0.14748799698747922 No Hit AGCGAGGGGAGCAGGCCTGCACCACGGAGCTCGCCCTGCGGCTGCAGCAAGTGCAGAG 94 0.14748799698747922 No Hit AGCGAGGGGGATCTTTGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAG 94 0.14748799698747922 No Hit AGCGAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGA 93 0.14591897574293156 No Hit AGCGAGGGGAGGCAAGAAATTGCGCCCTGAAAGACTGGCTGACTATGAGGCCCAAAAG 92 0.14434995449838392 No Hit AGCGAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC 89 0.13964289076474096 No Hit AGCGAGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAGA 89 0.13964289076474096 No Hit AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG 87 0.13650484827564566 No Hit AGCGAGGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCG 86 0.134935827031098 No Hit AGCGAGGGGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 86 0.134935827031098 No Hit AGCGAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA 81 0.12709072080835973 No Hit AGCGAGGGGACCAAGAAACGCTATCAGATAAAGTGACTCCAGAAGATGCCTCTTCTCA 80 0.1255216995638121 No Hit AGCGAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 79 0.12395267831926443 No Hit AGCGAGGGGGGAGAAACCAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCTCTGCGGA 79 0.12395267831926443 No Hit AGCGAGGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCGGAGTTA 79 0.12395267831926443 No Hit AGCGAGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGATGAAGC 78 0.1223836570747168 No Hit AGCGAGGGGGAGTGGGTTCTTGGTTTTAACCATGACCCACATTCTCACTTGAGTGTTG 78 0.1223836570747168 No Hit AGCGAGGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTCTGCGCT 78 0.1223836570747168 No Hit AGCGAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 77 0.12081463583016915 No Hit AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGTTTTCTCCT 77 0.12081463583016915 No Hit AGCGAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA 75 0.11767659334107385 No Hit AGCGAGGGGCAGCTCCTGTCTCCCAACTATATAGTAATCATTAGTAGACTGTTGCCTT 72 0.11296952960743087 No Hit AGCGAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 72 0.11296952960743087 No Hit AGCGAGGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGGTC 71 0.11140050836288323 No Hit AGCGAGGGGCGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCAG 71 0.11140050836288323 No Hit AGCGAGGGGGGTAGATTATCCACCCTGCAGTCGGGTCGGCTGGGGATAGTCAAGGGGG 70 0.1098314871183356 No Hit AGCGAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGT 70 0.1098314871183356 No Hit AGCGAGGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA 69 0.10826246587378793 No Hit AGCGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 67 0.10512442338469262 No Hit AGCGAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT 67 0.10512442338469262 No Hit AGCGAGGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAGGT 67 0.10512442338469262 No Hit AGCGAGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG 66 0.10355540214014498 No Hit AGCGAGGGGGGCCGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTT 66 0.10355540214014498 No Hit AGCGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 64 0.10041735965104967 No Hit AGCGAGGGGGCACAGGGCAAAGATGGCGGCGGAGGAGCAGTAGGCGTTGAGGGAGTTC 64 0.10041735965104967 No Hit AGCGAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGGACGCAGGTGTTGTGAAAACCA 64 0.10041735965104967 No Hit AGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 64 0.10041735965104967 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.006276084978190604 0.0 0.0 0.0 0.0 12 0.006276084978190604 0.0 0.0 0.0 0.0 13 0.006276084978190604 0.0 0.0 0.0 0.0 14 0.009414127467285907 0.0 0.0 0.0 0.0 15 0.012552169956381209 0.0 0.0 0.0 0.0 16 0.012552169956381209 0.0 0.0 0.0 0.0 17 0.012552169956381209 0.0 0.0 0.0 0.0 18 0.012552169956381209 0.0 0.0 0.0 0.0 19 0.012552169956381209 0.0 0.0 0.0 0.0 20 0.01412119120092886 0.0 0.0 0.0 0.0 21 0.01412119120092886 0.0 0.0 0.0 0.0 22 0.01412119120092886 0.0 0.0 0.0 0.0 23 0.015690212445476512 0.0 0.0 0.0 0.0 24 0.015690212445476512 0.0 0.0 0.0 0.0 25 0.015690212445476512 0.0 0.0 0.0 0.0 26 0.015690212445476512 0.0 0.0 0.0 0.0 27 0.015690212445476512 0.0 0.0 0.0 0.0 28 0.020397276179119465 0.0 0.0 0.0 0.0 29 0.020397276179119465 0.0 0.0 0.0 0.0 30 0.02667336115731007 0.0 0.0 0.0 0.0 31 0.02667336115731007 0.0 0.0 0.0 0.0 32 0.02824238240185772 0.0 0.0 0.0 0.0 33 0.02824238240185772 0.0 0.0 0.0 0.0 34 0.029811403646405374 0.0 0.0 0.0 0.0 35 0.045501616091881886 0.0 0.0 0.0 0.0 36 0.050208679825524835 0.0 0.0 0.0 0.0 37 0.050208679825524835 0.0 0.0 0.0 0.0 38 0.05177770107007249 0.0 0.0 0.0 0.0 39 0.05334672231462014 0.0 0.0 0.0 0.0 40 0.05334672231462014 0.0 0.0 0.0 0.0 41 0.05334672231462014 0.0 0.0 0.0 0.0 42 0.05334672231462014 0.0 0.0 0.0 0.0 43 0.05491574355916779 0.0 0.0 0.0 0.0 44 0.05648476480371544 0.0 0.0 0.0 0.0 45 0.0611918285373584 0.0 0.0 0.0 0.0 46 0.06276084978190605 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGGTA 140 0.0 52.43572 5 GGATGTT 15 0.006955348 52.435715 8 GGATGTA 15 0.006955348 52.435715 8 GGATGGA 20 3.284563E-4 52.435715 8 GTCATTT 20 3.284563E-4 52.435715 10 GGACTTT 15 0.006955348 52.435715 8 GGCGTGA 15 0.006955348 52.435715 9 GGGTACC 75 0.0 52.435715 7 GGTTAGT 15 0.006955348 52.435715 9 AGTGTAA 20 3.284563E-4 52.435715 10 GGGACGT 25 1.5583626E-5 52.435715 8 GGGATGT 40 1.715307E-9 52.435715 7 GGGACAG 20 3.284563E-4 52.435715 7 GGGATAT 15 0.006955348 52.435715 7 GGGGCGA 25 1.5583626E-5 52.435715 6 GGTGGAA 40 1.715307E-9 52.435715 9 GGGGTAC 90 0.0 52.435715 6 GCTTCTT 20 3.284563E-4 52.435715 10 GGGTGAT 15 0.006955348 52.435715 7 GGTACAT 15 0.006955348 52.435715 8 >>END_MODULE