FastQCFastQC Report
Tue 31 May 2016
SRR212544_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212544_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61325
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAGGGGACCCTTTCTCTAGAAGGTGCTAGTGGACATTTAAGATCTTCCTTGAT18142.9580105992662045No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC13302.16877293110477No Hit
AGCGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA10531.717081125152874No Hit
AGCGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA8671.4137790460660415No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC7301.1903791275988584No Hit
AGCGAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC6371.0387280880554424No Hit
AGCGAGGGGTACCTCACTCTTTACGTACGTGGTGACTGTTCGCACAGTCTGTGCTG5080.8283734203016714No Hit
AGCGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4540.7403179779861394No Hit
AGCGAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3930.6408479412963718No Hit
AGCGAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3650.5951895637994292No Hit
AGCGAGGGGGTGGAAGTAATATCTAATCGCTCAAAGTCCAAAAAAAAAACAATAAT3520.5739910313901345No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3120.5087647778230737No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC3000.48919690175295555No Hit
AGCGAGGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCC2910.4745209947003669No Hit
AGCGAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2830.4614757439869548No Hit
AGCGAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2690.4386465552384835No Hit
AGCGAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2680.437015898899307No Hit
AGCGAGGGGGTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGCTAC2530.41255605381165916No Hit
AGCGAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT2000.3261312678353037No Hit
AGCGAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1900.30982470444353855No Hit
AGCGAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA1820.2967794537301264No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1700.27721157766000815No Hit
AGCGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.2527517325723604No Hit
AGCGAGGGGGGCGGCGCGGTCGACTACCGGGCGAAGGCCGCGGAGCAGCGTCACCT1540.25112107623318386No Hit
AGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1400.22829188748471257No Hit
AGCGAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC1380.22503057480635957No Hit
AGCGAGGGGGAGTGGGTTCTTGGTTTTAACCATGACCCACATTCTCACTTGAGTGT1350.22013860578883002No Hit
AGCGAGGGGGGGAGTTGGAGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTCTTC1310.21361598043212393No Hit
AGCGAGGGGGCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1250.20383204239706482No Hit
AGCGAGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCC1230.20057072971871176No Hit
AGCGAGGGGGTTAGTGCTAGTGGTTCTGTTTTGTTTTTGTTTTTTGTTTTTTAAGG1190.1940481043620057No Hit
AGCGAGGGGAGGCAAGAAATTGCGCCCTGAAAGACTGGCTGACTATGAGGCCCAAA1180.19241744802282917No Hit
AGCGAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT1150.18752547900529964No Hit
AGCGAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC1140.18589482266612312No Hit
AGCGAGGGGCAATCTACCTGGATCTGCTGATGCTCAAGTGTTGAAATTACAGATGT1130.1842641663269466No Hit
AGCGAGGGGAACCAAAAGGATGTGGGGTTTTTCCTTGGGTTATGTCTCCTTATTCA1100.17937219730941703No Hit
AGCGAGGGGACCAAGAAACGCTATCAGATAAAGTGACTCCAGAAGATGCCTCTTCT1090.17774154097024053No Hit
AGCGAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1080.176110884631064No Hit
AGCGAGGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTC1070.1744802282918875No Hit
AGCGAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1050.17121891561353444No Hit
AGCGAGGGGGTCATTTTATTTCAAAGTAAAGATGGTGACAGTAGCCAGAAATCTTT1010.16469629025682836No Hit
AGCGAGGGGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980.1598043212392988No Hit
AGCGAGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG960.15654300856094577No Hit
AGCGAGGGGGATCTTTGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAG930.15165103954341622No Hit
AGCGAGGGGGGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGG870.1418671015083571No Hit
AGCGAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC860.1402364451691806No Hit
AGCGAGGGGGACTTCCGGGTTGGACACTGGGACGGCAGAGGAGGAGAGGAAATTGG850.13860578883000407No Hit
AGCGAGGGGGGAGAAACCAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCTCTGCG820.13371381981247452No Hit
AGCGAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC810.13208316347329802No Hit
AGCGAGGGGAGATACTTCCTGCTATAGTTGATGTCTTTGGAAAGGAGCCAGGTTTT790.12882185079494496No Hit
AGCGAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC770.12556053811659193No Hit
AGCGAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCT760.12392988177741542No Hit
AGCGAGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCA750.12229922543823889No Hit
AGCGAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC710.11577660008153283No Hit
AGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.11577660008153283No Hit
AGCGAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC700.11414594374235629No Hit
AGCGAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC690.11251528740317979No Hit
AGCGAGGGGTATATTAAAAATGGACCTATTTTAATACTGTTCTGTCAGGAAAATGT680.11088463106400327No Hit
AGCGAGGGGGAGTGGGCGGTGTGGGCGGGGCATGCGTGAAAGGGTGAGGTGCGTCC670.10925397472482674No Hit
AGCGAGGGGCGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCC670.10925397472482674No Hit
AGCGAGGGGGTGGAAGTAATATCTAATCGCTCAAAGTCCAAAAAAAAACAATAATG660.10762331838565023No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC660.10762331838565023No Hit
AGCGAGGGGCAGCTCCTGTCTCCCAACTATATAGTAATCATTAGTAGACTGTTGCC650.1059926620464737No Hit
AGCGAGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA640.10436200570729719No Hit
AGCGAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCT640.10436200570729719No Hit
AGCGAGGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCGAG640.10436200570729719No Hit
AGCGAGGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG630.10273134936812067No Hit
AGCGAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG620.10110069302894414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTTT150.00822055350.26315750
GTCACCT150.00822055350.26315750
AAATTGG150.00822055350.26315750
AAACAAC150.00822055350.26315750
GTCGTGG150.00822055350.26315750
TTATCTC204.0627646E-450.2218647
ATTATCT204.0627646E-450.2218646
TTCTCTG204.0627646E-450.2218649
AAGATCT2600.050.2218642
GATCTTC2650.050.2218644
CCATTCT204.0627646E-450.2218644
AGATCTT2600.050.2218643
CGTCACC150.00824733850.22185549
TTCACCC150.00824733850.22185549
AGCTTGT150.00824733850.22185548
TAAATCT150.00824733850.22185548
AAATCTT252.0106818E-550.22185549
CCAGCTT150.00824733850.22185543
CTCCTTA150.00824733850.22185546
TACAGAT150.00824733850.22185548
Produced by FastQC (version 0.11.5)