SRR212543_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212543_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	57262
Sequences flagged as poor quality	0
Sequence length	56
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGCGAGGGGACCCTTTCTCTAGAAGGTGCTAGTGGACATTTAAGATCTTCCTTGAT	1587	2.771471481960113	No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	1360	2.37504802486815	No Hit
AGCGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	1003	1.7515979183402606	No Hit
AGCGAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC	868	1.5158394746952604	No Hit
AGCGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	806	1.4075652265027419	No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	636	1.1106842233942231	No Hit
AGCGAGGGGTACCTCACTCTTTACGTACGTGGTGACTGTTCGCACAGTCTGTGCTG	501	0.8749257797492228	No Hit
AGCGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	475	0.8295204498620377	No Hit
AGCGAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	367	0.6409136949460376	No Hit
AGCGAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	355	0.6199573888442598	No Hit
AGCGAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	305	0.5326394467535189	No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	300	0.5239076525444448	No Hit
AGCGAGGGGGTGGAAGTAATATCTAATCGCTCAAAGTCCAAAAAAAAAACAATAAT	298	0.5204149348608151	No Hit
AGCGAGGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCC	295	0.5151758583353707	No Hit
AGCGAGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	279	0.4872341168663337	No Hit
AGCGAGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	267	0.46627781076455593	No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC	263	0.4592923753972966	No Hit
AGCGAGGGGGTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGCTAC	247	0.43135063392825956	No Hit
AGCGAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA	192	0.33530089762844467	No Hit
AGCGAGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	190	0.33180817994481504	No Hit
AGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	180	0.3143445915266669	No Hit
AGCGAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCTTCT	174	0.303866438475778	No Hit
AGCGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	169	0.29513464426670394	No Hit
AGCGAGGGGGGCGGCGCGGTCGACTACCGGGCGAAGGCCGCGGAGCAGCGTCACCT	161	0.28116377353218536	No Hit
AGCGAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT	152	0.26544654395585204	No Hit
AGCGAGGGGGAGTGGGTTCTTGGTTTTAACCATGACCCACATTCTCACTTGAGTGT	144	0.2514756732213335	No Hit
AGCGAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGGAGCGCAGGCCGCC	144	0.2514756732213335	No Hit
AGCGAGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCC	134	0.23401208480318536	No Hit
AGCGAGGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCC	133	0.23226572596137057	No Hit
AGCGAGGGGGCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG	131	0.2287730082777409	No Hit
AGCGAGGGGCAATCTACCTGGATCTGCTGATGCTCAAGTGTTGAAATTACAGATGT	128	0.22353393175229647	No Hit
AGCGAGGGGGGGAGTTGGAGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTCTTC	119	0.2078167021759631	No Hit
AGCGAGGGGGTCATTTTATTTCAAAGTAAAGATGGTGACAGTAGCCAGAAATCTTT	115	0.20083126680870383	No Hit
AGCGAGGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTC	115	0.20083126680870383	No Hit
AGCGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	107	0.1868603960741853	No Hit
AGCGAGGGGAGGCAAGAAATTGCGCCCTGAAAGACTGGCTGACTATGAGGCCCAAA	97	0.16939680765603718	No Hit
AGCGAGGGGGATCTTTGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAG	96	0.16765044881422234	No Hit
AGCGAGGGGGTTAGTGCTAGTGGTTCTGTTTTGTTTTTGTTTTTTGTTTTTTAAGG	96	0.16765044881422234	No Hit
AGCGAGGGGACCAAGAAACGCTATCAGATAAAGTGACTCCAGAAGATGCCTCTTCT	95	0.16590408997240752	No Hit
AGCGAGGGGGACTTCCGGGTTGGACACTGGGACGGCAGAGGAGGAGAGGAAATTGG	90	0.15717229576333344	No Hit
AGCGAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	86	0.15018686039607418	No Hit
AGCGAGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG	86	0.15018686039607418	No Hit
AGCGAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCT	83	0.14494778387062973	No Hit
AGCGAGGGGAACCAAAAGGATGTGGGGTTTTTCCTTGGGTTATGTCTCCTTATTCA	83	0.14494778387062973	No Hit
AGCGAGGGGAGATACTTCCTGCTATAGTTGATGTCTTTGGAAAGGAGCCAGGTTTT	83	0.14494778387062973	No Hit
AGCGAGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGGGGGCCCAAGAAACA	82	0.14320142502881492	No Hit
AGCGAGGGGGCTTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGA	82	0.14320142502881492	No Hit
AGCGAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	79	0.13796234850337047	No Hit
AGCGAGGGGGGCGGTGATGGGCAGCATTTATCAGTATATCCACGTGGCGTTTCAGG	78	0.13621598966155565	No Hit
AGCGAGGGGGAGTGGGCGGTGTGGGCGGGGCATGCGTGAAAGGGTGAGGTGCGTCC	75	0.1309769131361112	No Hit
AGCGAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC	75	0.1309769131361112	No Hit
AGCGAGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC	74	0.1292305542942964	No Hit
AGCGAGGGGGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCCA	70	0.12224511892703713	No Hit
AGCGAGGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGTCGTGG	69	0.1204987600852223	No Hit
AGCGAGGGGTATATTAAAAATGGACCTATTTTAATACTGTTCTGTCAGGAAAATGT	69	0.1204987600852223	No Hit
AGCGAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG	68	0.1187524012434075	No Hit
AGCGAGGGGGGCCATTTTCGAGCGCCCTCTTTCAAAATGGCAGCTCGAAGGAAGTT	67	0.11700604240159268	No Hit
AGCGAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	67	0.11700604240159268	No Hit
AGCGAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC	66	0.11525968355977785	No Hit
AGCGAGGGGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64	0.11176696587614823	No Hit
AGCGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63	0.1100206070343334	No Hit
AGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC	63	0.1100206070343334	No Hit
AGCGAGGGGGCTCTTTCCGCCATCTTGGCGCCTGTGGAGGCCTGCTGGGAACAGGA	61	0.10652788935070379	No Hit
AGCGAGGGGGTGGAAGTAATATCTAATCGCTCAAAGTCCAAAAAAAAACAATAATG	61	0.10652788935070379	No Hit
AGCGAGGGGGGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGGTTC	59	0.10303517166707415	No Hit
AGCGAGGGGCGAGCTGGAGAGATGGCTCAGCGGTTTGGAACACTGACTGTTCTTCC	59	0.10303517166707415	No Hit
AGCGAGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC	59	0.10303517166707415	No Hit
AGCGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGTTTTCTC	58	0.10128881282525934	No Hit
AGCGAGGGGGGCCGTGCGTGGTGGATAAGCTTGATCTCGTCTTCCCTGAAGTCTGG	58	0.10128881282525934	No Hit
AGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58	0.10128881282525934	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAACAC	20	3.913264E-4	50.598225	50
CAGATGT	20	3.913264E-4	50.598225	50
GCCCAAA	25	1.9219313E-5	50.59822	50
CCTCGGC	15	0.008004375	50.59822	50
AAATACG	15	0.008004375	50.59822	50
TGATATG	30	9.4892494E-7	50.59822	50
TCCTCGG	15	0.008032573	50.553288	49
ACAGATG	20	3.9304886E-4	50.553288	49
AAATCTT	30	9.54773E-7	50.553288	49
GAAATAC	15	0.008032573	50.553288	49
TTGAGTG	20	3.9304886E-4	50.553288	49
CTTATTC	20	3.9304886E-4	50.553288	49
TCTTGTG	15	0.008032573	50.553288	49
TACTGAA	15	0.008032573	50.553288	49
CAGGTTT	15	0.008032573	50.553288	49
GTGCGTC	15	0.008032573	50.553288	49
ACCAGCA	15	0.008032573	50.553288	49
CTGTGCT	50	5.456968E-12	50.553284	49
GAGGTGC	15	0.00808919	50.463654	46
GGCACTT	15	0.00808919	50.463654	47

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content