SRR212542_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR212542_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	88426
Sequences flagged as poor quality	0
Sequence length	58
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGTACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	4254	4.810802252731097	No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA	1478	1.6714540972112275	No Hit
GGTACAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	1417	1.6024698618053514	No Hit
GGTACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	1405	1.588899192545179	No Hit
GGTACAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	1099	1.242847126410784	No Hit
GGTACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG	1068	1.207789564155339	No Hit
GGTACAGGGATTGAACACCCGCACGTGCTCAAGCTCCACGACGTCTACGAGAACAAGA	758	0.8572139416008866	No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	742	0.8391197159206567	No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA	592	0.6694863501685024	No Hit
GGTACAGGGGTTTTTAGAAGTTATTAAACCTTTCTGTGCAGTTCTACCAGAAATTCAG	579	0.6547847918033158	No Hit
GGTACAGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC	491	0.5552665505620519	No Hit
GGTACAGGGGGCGCATGAGCTCTGACTTCTACAGGCCTTCCACACGAGGCGACATGAA	368	0.41616719064528535	No Hit
GGTACAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	330	0.37319340465473955	No Hit
GGTACAGGGCGGATTTACCAGGCTTTTCTCATGTCGGCCACTTTCAATGAGGATGTAC	307	0.34718295523940923	No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	307	0.34718295523940923	No Hit
GGTACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	299	0.33813584239929434	No Hit
GGTACAGGGGGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCT	292	0.3302196186641938	No Hit
GGTACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT	270	0.3053400583538778	No Hit
GGTACAGGGCGCGGTTCCAGCTTTATGTAAACACACACACACCCCAGTTATAGTTCTA	262	0.29629294551376295	No Hit
GGTACAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG	234	0.26462805057336075	No Hit
GGTACAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC	220	0.24879560310315968	No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA	209	0.2363558229480017	No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA	204	0.23070137742292993	No Hit
GGTACAGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCA	203	0.22957048831791557	No Hit
GGTACAGGGCTGTGGTGACTTTTGTTTGCAACTGTAATAAGTTATAAACTGCATGATT	203	0.22957048831791557	No Hit
GGTACAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA	193	0.21826159726777195	No Hit
GGTACAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT	183	0.2069527062176283	No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG	182	0.20582181711261394	No Hit
GGTACAGGGAGATAGAGTCTTAAGAGAGATGATATCGTACTTTTTGTAGAACGGGCCA	182	0.20582181711261394	No Hit
GGTACAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA	181	0.20469092800759958	No Hit
GGTACAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	180	0.20356003890258523	No Hit
GGTACAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC	174	0.19677470427249905	No Hit
GGTACAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT	172	0.1945129260624703	No Hit
GGTACAGGGGTTCTATTTTTCTAATAGTCTTCTTACAGTTTCTTATAATGATGACATT	170	0.19225114785244157	No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCGGA	166	0.18772759143238413	No Hit
GGTACAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA	166	0.18772759143238413	No Hit
GGTACAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	164	0.18546581322235542	No Hit
GGTACAGGGGGGAATCCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC	162	0.18320403501232668	No Hit
GGTACAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	157	0.17754958948725488	No Hit
GGTACAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	152	0.17189514396218308	No Hit
GGTACAGGGCACACCTTACACTGCACTGTAGCTTGGTCTCTAACCACAGTTAGAAGCC	148	0.1673715875421256	No Hit
GGTACAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC	146	0.1651098093320969	No Hit
GGTACAGGGATCTTTCATGTGTCCCCTTTGGGCCTGGGTAGGTCTTTCTTTGGTGTGC	143	0.1617171420170538	No Hit
GGTACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT	142	0.16058625291203943	No Hit
GGTACAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG	136	0.15380091828195327	No Hit
GGTACAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	127	0.14362291633682403	No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG	122	0.1379684708117522	No Hit
GGTACAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA	120	0.13570669260172347	No Hit
GGTACAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	119	0.1345758034967091	No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA	119	0.1345758034967091	No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAG	114	0.12892135797163728	No Hit
GGTACAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA	113	0.12779046886662293	No Hit
GGTACAGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC	111	0.12552869065659422	No Hit
GGTACAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG	110	0.12439780155157984	No Hit
GGTACAGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG	109	0.12326691244656549	No Hit
GGTACAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG	109	0.12326691244656549	No Hit
GGTACAGGGGGGGCTGGTGAGATAGCACAGTGGGTAAGAGCACCCAACTGCTCTTCTG	109	0.12326691244656549	No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC	109	0.12326691244656549	No Hit
GGTACAGGGGTCCTACCGTCCCCTCACTAGAGCTTCTTGTACCCTACTCCATCTCTAG	107	0.12100513423653676	No Hit
GGTACAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105	0.11874335602650803	No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA	104	0.11761246692149367	No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGCTGGTAAGAGCACTGACTGCTCTTCCAAA	103	0.1164815778164793	No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA	99	0.11195802139642187	No Hit
GGTACAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA	98	0.11082713229140752	No Hit
GGTACAGGGCGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA	98	0.11082713229140752	No Hit
GGTACAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG	97	0.10969624318639315	No Hit
GGTACAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAA	95	0.10743446497636443	No Hit
GGTACAGGGATTTCCATTCTAGAACCATCCTTTGTTCTTCTTGTTTCAGTTTTTACAA	95	0.10743446497636443	No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCA	94	0.10630357587135006	No Hit
GGTACAGGGGGCTGGCTCCGGCCGCCGAGATGAGCTGGGGCCGTCTGAGCCGCTTACT	93	0.10517268676633569	No Hit
GGTACAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACACC	93	0.10517268676633569	No Hit
GGTACAGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA	93	0.10517268676633569	No Hit
GGTACAGGGGCTTTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA	92	0.10404179766132134	No Hit
GGTACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	90	0.10178001945129261	No Hit
GGTACAGGGGAGAGGGAGGTCGCACACTCTGAGTTTCGGTGACCCGGAAGGGAGCCCC	90	0.10178001945129261	No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAATGGTTAAGAGCACTGAGCACTGACTGCT	89	0.10064913034627825	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATGAG	35	3.8055077E-8	52.040207	45
CCAATGG	20	3.417182E-4	52.040207	43
GTTTGAT	20	3.417182E-4	52.040207	48
AATCTCC	25	1.6351829E-5	52.040207	51
GTCACCA	40	1.8480932E-9	52.040207	46
CAGTTAG	25	1.6351829E-5	52.040207	47
GTCATCA	20	3.417182E-4	52.040207	46
ACGTCTA	70	0.0	52.040207	41
AGAAGCC	25	1.6351829E-5	52.040207	52
CTCCCCG	25	1.6351829E-5	52.040207	49
AGGATGT	35	3.8055077E-8	52.040207	50
TGCGAGT	20	3.417182E-4	52.040207	46
CCTGATA	35	3.8055077E-8	52.040207	48
TTTCAAT	35	3.8055077E-8	52.040207	42
TATTGTC	20	3.417182E-4	52.040207	42
TAATGAT	25	1.6351829E-5	52.040207	46
TCTACGA	70	0.0	52.040207	44
TTATAAT	25	1.6351829E-5	52.040207	43
ATAATGA	25	1.6351829E-5	52.040207	45
CCCGTCA	25	1.6351829E-5	52.040207	52

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content