FastQCFastQC Report
Tue 31 May 2016
SRR212541_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212541_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88504
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT40664.594142637620899No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA13761.5547319895145981No Hit
GGTACAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC13541.5298743559613126No Hit
GGTACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC13321.5050167224080269No Hit
GGTACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG10951.237232215493085No Hit
GGTACAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT10781.2180240441110006No Hit
GGTACAGGGATTGAACACCCGCACGTGCTCAAGCTCCACGACGTCTACGAGAACAAGA7100.8022236283105848No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG7090.8010937358763446No Hit
GGTACAGGGGTTTTTAGAAGTTATTAAACCTTTCTGTGCAGTTCTACCAGAAATTCAG5800.655337611859351No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA5200.5875440658049353No Hit
GGTACAGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4250.48020428455211067No Hit
GGTACAGGGGGCGCATGAGCTCTGACTTCTACAGGCCTTCCACACGAGGCGACATGAA3600.4067612763264937No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3090.3491367621802404No Hit
GGTACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3050.3446171924432794No Hit
GGTACAGGGCGGATTTACCAGGCTTTTCTCATGTCGGCCACTTTCAATGAGGATGTAC2920.329928590798156No Hit
GGTACAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2750.3107204194160716No Hit
GGTACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT2510.28360300099430535No Hit
GGTACAGGGGGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCT2510.28360300099430535No Hit
GGTACAGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCA2390.2700442917834222No Hit
GGTACAGGGCGCGGTTCCAGCTTTATGTAAACACACACACACCCCAGTTATAGTTCTA2340.26439482961222094No Hit
GGTACAGGGAGATAGAGTCTTAAGAGAGATGATATCGTACTTTTTGTAGAACGGGCCA2100.23727741119045467No Hit
GGTACAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC2100.23727741119045467No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA2090.23614751875621443No Hit
GGTACAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG1980.22371870197957153No Hit
GGTACAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA1930.21806923980837023No Hit
GGTACAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1900.21467956250564948No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG1850.20903010033444816No Hit
GGTACAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1790.2022507457290066No Hit
GGTACAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1740.1966012835578053No Hit
GGTACAGGGCTGTGGTGACTTTTGTTTGCAACTGTAATAAGTTATAAACTGCATGATT1740.1966012835578053No Hit
GGTACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT1730.195471391123565No Hit
GGTACAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1730.195471391123565No Hit
GGTACAGGGGTTCTATTTTTCTAATAGTCTTCTTACAGTTTCTTATAATGATGACATT1690.190951821386604No Hit
GGTACAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.18756214408388322No Hit
GGTACAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1620.18304257434692217No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1620.18304257434692217No Hit
GGTACAGGGCACACCTTACACTGCACTGTAGCTTGGTCTCTAACCACAGTTAGAAGCC1620.18304257434692217No Hit
GGTACAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1580.17852300460996115No Hit
GGTACAGGGGGGAATCCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC1550.17513332730724035No Hit
GGTACAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1550.17513332730724035No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCGGA1520.17174365000451958No Hit
GGTACAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG1510.1706137575702793No Hit
GGTACAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1500.16948386513603905No Hit
GGTACAGGGATCTTTCATGTGTCCCCTTTGGGCCTGGGTAGGTCTTTCTTTGGTGTGC1430.16157461809635723No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1420.16044472566211695No Hit
GGTACAGGGGGGGCTGGTGAGATAGCACAGTGGGTAAGAGCACCCAACTGCTCTTCTG1340.15140558618819489No Hit
GGTACAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1230.13897676941155201No Hit
GGTACAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAA1220.13784687697731174No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA1200.13558709210883124No Hit
GGTACAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.13332730724035072No Hit
GGTACAGGGGTCCTACCGTCCCCTCACTAGAGCTTCTTGTACCCTACTCCATCTCTAG1130.12767784506914942No Hit
GGTACAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1130.12767784506914942No Hit
GGTACAGGGGGCTGGCTCCGGCCGCCGAGATGAGCTGGGGCCGTCTGAGCCGCTTACT1080.12202838289794811No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA1070.12089849046370785No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAG1060.1197685980294676No Hit
GGTACAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAA1040.11750881316098707No Hit
GGTACAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.11637892072674681No Hit
GGTACAGGGCGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCA1020.11524902829250655No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGCTGGTAAGAGCACTGACTGCTCTTCCAAA1010.11411913585826629No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCC1000.11298924342402603No Hit
GGTACAGGGGAGAGGGAGGTCGCACACTCTGAGTTTCGGTGACCCGGAAGGGAGCCCC1000.11298924342402603No Hit
GGTACAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG990.11185935098978576No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG980.1107294585555455No Hit
GGTACAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA980.1107294585555455No Hit
GGTACAGGGGGGCTGGTGAGATGGCTTGGCGGTTAAGAGCACTGACTGCTCTTCCAGA980.1107294585555455No Hit
GGTACAGGGGGAACGAGGCTCCGGCACCATGTCTGGTTTGTTTGGCCCACTCTCCCGG970.10959956612130527No Hit
GGTACAGGGGGGGGGATGTTGGTTGTGTTTGGATATACGACTGCTATAGCTACTGAGG970.10959956612130527No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGA940.10620988881858448No Hit
GGTACAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG930.10507999638434422No Hit
GGTACAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC900.10169031908162343No Hit
GGTACAGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCA900.10169031908162343No Hit
GGTACAGGGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCAACTGCTCTTCCG890.10056042664738317No Hit
GGTACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC890.10056042664738317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTT150.00710044352.1800127
CAACCAC150.00710044352.1800127
CTGCGAG150.00710044352.1800127
CTTGCGG203.3721718E-452.1800127
TCTCATG203.3721718E-452.1800127
GAGATAA251.609387E-552.1800127
GTAAACA750.052.1800127
ATGTAAA750.052.150425
TAGCGCA307.7544246E-752.15039424
ACTGCGA150.007116412752.15039426
CCAACCA150.007116412752.15039426
GGCATCT150.007116412752.15039425
CCTTGCG203.3816457E-452.15039426
TAGTCTT203.3816457E-452.15039425
ACATCAA203.3816457E-452.15039425
ATAGTCT203.3816457E-452.15039424
GCACGCC150.007116412752.15039425
TACTGCG150.007116412752.15039425
CCGAGAT150.007116412752.15039425
CACTAGA251.614811E-552.15039425
Produced by FastQC (version 0.11.5)