FastQCFastQC Report
Tue 31 May 2016
SRR212540_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212540_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87666
Sequences flagged as poor quality0
Sequence length58
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT37594.2878653069605095No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA13391.5273880409736957No Hit
GGTACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC12441.4190221978874364No Hit
GGTACAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC12341.4076152670362512No Hit
GGTACAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT9821.1201606095863847No Hit
GGTACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG9171.046015559053681No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG6710.7654050601145256No Hit
GGTACAGGGATTGAACACCCGCACGTGCTCAAGCTCCACGACGTCTACGAGAACAAGA6270.7152145643693107No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA4980.5680651563890219No Hit
GGTACAGGGGTTTTTAGAAGTTATTAAACCTTTCTGTGCAGTTCTACCAGAAATTCAG4970.5669244633039034No Hit
GGTACAGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4250.4847945611753702No Hit
GGTACAGGGGGCGCATGAGCTCTGACTTCTACAGGCCTTCCACACGAGGCGACATGAA3270.3730066388337554No Hit
GGTACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2850.3250975292587776No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2770.3159719845778295No Hit
GGTACAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA2710.30912782606711836No Hit
GGTACAGGGCGGATTTACCAGGCTTTTCTCATGTCGGCCACTTTCAATGAGGATGTAC2550.2908767367052221No Hit
GGTACAGGGGGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCT2550.2908767367052221No Hit
GGTACAGGGCTGTGGTGACTTTTGTTTGCAACTGTAATAAGTTATAAACTGCATGATT2130.2429676271302443No Hit
GGTACAGGGCGCGGTTCCAGCTTTATGTAAACACACACACACCCCAGTTATAGTTCTA2050.23384208244929622No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGA2030.23156069627905917No Hit
GGTACAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTG2030.23156069627905917No Hit
GGTACAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2000.2281386170237036No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA1980.22585723085346657No Hit
GGTACAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1970.22471653776834805No Hit
GGTACAGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCA1910.21787237925763697No Hit
GGTACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT1890.2155909930873999No Hit
GGTACAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1830.2087468345766888No Hit
GGTACAGGGAGATAGAGTCTTAAGAGAGATGATATCGTACTTTTTGTAGAACGGGCCA1820.20760614149157025No Hit
GGTACAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1720.19619921064038512No Hit
GGTACAGGGGTTCTATTTTTCTAATAGTCTTCTTACAGTTTCTTATAATGATGACATT1660.189355052129674No Hit
GGTACAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1580.18022950744872585No Hit
GGTACAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGGT1500.1711039627677777No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCG1490.16996326968265918No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA1470.16768188351242216No Hit
GGTACAGGGCACACCTTACACTGCACTGTAGCTTGGTCTCTAACCACAGTTAGAAGCC1470.16768188351242216No Hit
GGTACAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT1360.15513425957611846No Hit
GGTACAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1350.15399356649099993No Hit
GGTACAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1340.1528528734058814No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCGGA1320.15057148723564437No Hit
GGTACAGGGGGGAATCCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC1290.14714940798028883No Hit
GGTACAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.14372732872493327No Hit
GGTACAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCGCG1150.1311797047886296No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCCGA1130.12889831861839254No Hit
GGTACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCT1120.12775762553327402No Hit
GGTACAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1110.1266169324481555No Hit
GGTACAGGGATCTTTCATGTGTCCCCTTTGGGCCTGGGTAGGTCTTTCTTTGGTGTGC1100.12547623936303698No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTCCG1050.11977277393744439No Hit
GGTACAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCC1050.11977277393744439No Hit
GGTACAGGGGTCCTACCGTCCCCTCACTAGAGCTTCTTGTACCCTACTCCATCTCTAG1040.11863208085232588No Hit
GGTACAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1040.11863208085232588No Hit
GGTACAGGGGGGGCTGGTGAGATAGCACAGTGGGTAAGAGCACCCAACTGCTCTTCTG1030.11749138776720736No Hit
GGTACAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG1030.11749138776720736No Hit
GGTACAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAA1020.11635069468208883No Hit
GGTACAGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA1000.1140693085118518No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA1000.1140693085118518No Hit
GGTACAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980.11178792234161476No Hit
GGTACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC980.11178792234161476No Hit
GGTACAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.11064722925649625No Hit
GGTACAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGT960.10950653617137773No Hit
GGTACAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC950.10836584308625921No Hit
GGTACAGGGGGCTGGCTCCGGCCGCCGAGATGAGCTGGGGCCGTCTGAGCCGCTTACT950.10836584308625921No Hit
GGTACAGGGGGGGCTGAAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAG950.10836584308625921No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAG930.10608445691602218No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAATGGTTAAGAGCACTGAGCACTGACTGCT930.10608445691602218No Hit
GGTACAGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACAGAAG890.10152168457554811No Hit
GGTACAGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGC880.10038099149042957No Hit
GGTACAGGGGGAACGAGGCTCCGGCACCATGTCTGGTTTGTTTGGCCCACTCTCCCGG880.10038099149042957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGA150.006970812652.4230589
GGGTCTA150.006970812652.4230588
GGGTTGA150.006970812652.4230588
GGGTTCT203.2954224E-452.4230588
GGCACAC203.2954224E-452.4230588
GACAGTG458.367351E-1152.4230589
GAAGTGT150.006970812652.4230589
AGGGCGC203.2954224E-452.4230586
AGGGCCA307.4790114E-752.4230586
GGTTCTA150.006970812652.4230589
AGGGTCC150.006970812652.4230586
AGGGTAT458.367351E-1152.4230586
GGATTGA800.052.4230588
CGGATTT401.7316779E-952.42305810
GAATGTA150.006970812652.4230589
GCGGATT401.7316779E-952.4230589
AGGGAAC850.052.4230586
GGGAGAA203.2954224E-452.4230588
CTGTGGT401.7316779E-952.42305810
AATGGTA203.2954224E-452.42305810
Produced by FastQC (version 0.11.5)