FastQCFastQC Report
Tue 31 May 2016
SRR212539_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212539_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83817
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC31693.780855912285097No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC18792.2417886586253384No Hit
GGTACAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT10551.2586945369077873No Hit
GGTACAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA10221.2193230490234679No Hit
GGTACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC9791.1680208072348093No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC9281.1071739623226793No Hit
GGTACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA8360.9974110264027584No Hit
GGTACAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC7100.8470835272080843No Hit
GGTACAGGGGTTTTTAGAAGTTATTAAACCTTTCTGTGCAGTTCTACCAGAAATTC5490.6549983893482229No Hit
GGTACAGGGATTGAACACCCGCACGTGCTCAAGCTCCACGACGTCTACGAGAACAA5210.6215922784160731No Hit
GGTACAGGGGGCGCATGAGCTCTGACTTCTACAGGCCTTCCACACGAGGCGACATG4390.5237600964004915No Hit
GGTACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC4350.5189877948387558No Hit
GGTACAGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC3790.4521755729744562No Hit
GGTACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.38417027571972273No Hit
GGTACAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA2630.31377882768412135No Hit
GGTACAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2470.2946896214371786No Hit
GGTACAGGGCGCGGTTCCAGCTTTATGTAAACACACACACACCCCAGTTATAGTTC2430.28991731987544295No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2340.2791796413615376No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2320.2767934905806698No Hit
GGTACAGGGCGGATTTACCAGGCTTTTCTCATGTCGGCCACTTTCAATGAGGATGT2260.2696350382380662No Hit
GGTACAGGGCTGTGGTGACTTTTGTTTGCAACTGTAATAAGTTATAAACTGCATGA2260.2696350382380662No Hit
GGTACAGGGGGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGC2030.24219430425808608No Hit
GGTACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2000.2386150780867843No Hit
GGTACAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1720.2052089671546345No Hit
GGTACAGGGGTTCTATTTTTCTAATAGTCTTCTTACAGTTTCTTATAATGATGACA1710.20401589176420057No Hit
GGTACAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC1680.20043666559289883No Hit
GGTACAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1640.19566436403116316No Hit
GGTACAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1630.1944712886407292No Hit
GGTACAGGGAGATAGAGTCTTAAGAGAGATGATATCGTACTTTTTGTAGAACGGGC1610.19208513785986137No Hit
GGTACAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1610.19208513785986137No Hit
GGTACAGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC1610.19208513785986137No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1540.18373361012682393No Hit
GGTACAGGGACCGTGGTATCTTTATTGATGACATGTGTAGAGAAAGCAGGAACCGA1510.18015438395552213No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC1500.1789613085650882No Hit
GGTACAGGGCACACCTTACACTGCACTGTAGCTTGGTCTCTAACCACAGTTAGAAG1480.1765751577842204No Hit
GGTACAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA1460.17418900700335255No Hit
GGTACAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.17299593161291862No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC1430.17060978083205078No Hit
GGTACAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1430.17060978083205078No Hit
GGTACAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1410.16822363005118293No Hit
GGTACAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1390.16583747927031509No Hit
GGTACAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG1380.16464440387988116No Hit
GGTACAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG1310.15629287614684373No Hit
GGTACAGGGATCTACGTCTGTCTGTGGAGATAATTGTAGCTGTACTCCGTCTCTAC1300.1550998007564098No Hit
GGTACAGGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT1300.1550998007564098No Hit
GGTACAGGGATCTTTCATGTGTCCCCTTTGGGCCTGGGTAGGTCTTTCTTTGGTGT1290.15390672536597588No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC1200.14316904685207058No Hit
GGTACAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG1180.14078289607120273No Hit
GGTACAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATC1160.1383967452903349No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCG1160.1383967452903349No Hit
GGTACAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.137203669899901No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC1150.137203669899901No Hit
GGTACAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1150.137203669899901No Hit
GGTACAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT1140.13601059450946706No Hit
GGTACAGGGGGGAATCCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1120.1336244437285992No Hit
GGTACAGGGAGCTAGAGTCAAGAGCTCCGGGGTACTGGTTAGCTCATAATGTTGTT1090.13004521755729745No Hit
GGTACAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.1276590667764296No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT1060.12646599138599568No Hit
GGTACAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA1050.12527291599556176No Hit
GGTACAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1040.12407984060512783No Hit
GGTACAGGGATTGTGAACTTCGTTCACACCAACTTGAGGAAAAACAACAGACAGCC1030.12288676521469391No Hit
GGTACAGGGGGGGGGATGTTGGTTGTGTTTGGATATACGACTGCTATAGCTACTGA1030.12288676521469391No Hit
GGTACAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT1010.12050061443382608No Hit
GGTACAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1010.12050061443382608No Hit
GGTACAGGGGGGGCTGGTGAGATAGCACAGTGGGTAAGAGCACCCAACTGCTCTTC990.11811446365295823No Hit
GGTACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTC980.11692138826252432No Hit
GGTACAGGGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCAACTGCTCTTC970.11572831287209037No Hit
GGTACAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC950.11334216209122254No Hit
GGTACAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA950.11334216209122254No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTT940.11214908670078862No Hit
GGTACAGGGGTCCTACCGTCCCCTCACTAGAGCTTCTTGTACCCTACTCCATCTCT930.11095601131035471No Hit
GGTACAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGA930.11095601131035471No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC920.10976293591992078No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGCTGGTAAGAGCACTGACTGCTCTTCCA890.106183709748619No Hit
GGTACAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG890.106183709748619No Hit
GGTACAGGGCGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT880.10499063435818509No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAATGGTTAAGAGCACTGAGCACTGACTG880.10499063435818509No Hit
GGTACAGGGATTTCCATTCTAGAACCATCCTTTGTTCTTCTTGTTTCAGTTTTTAC880.10499063435818509No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC840.10021833279644941No Hit
GGTACAGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCC840.10021833279644941No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCATGA251.990749E-550.33353450
GAAATTC550.050.33353450
TCGCCGT150.00818565650.33353450
GAACCGA309.896157E-750.33353450
TATCACT204.0270033E-450.33353450
CGACTGA150.00818565650.33353450
CAAGCCC204.0270033E-450.33353450
ATGATGA354.96766E-850.303248
ACTGCAT251.9978725E-550.30319648
GCAAGCC204.0390156E-450.30319649
ACTCTTC204.0390156E-450.30319649
AGAAATT505.456968E-1250.30319649
GTTAGAA150.00820520450.30319649
CAGACAG150.00820520450.30319648
TCTCCCC150.00820520450.30319648
ACGGCCT150.00820520450.30319649
GGAACCG309.937467E-750.30319649
ACTATCA204.0390156E-450.30319648
AGTTAGA150.00820520450.30319648
TATAATG354.9914888E-850.27289644
Produced by FastQC (version 0.11.5)