##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212539_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 83817 Sequences flagged as poor quality 0 Sequence length 56 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.335588245821256 38.0 37.0 40.0 33.0 40.0 2 36.89873176085997 38.0 36.0 39.0 33.0 40.0 3 34.78320627080425 36.0 33.0 39.0 27.0 40.0 4 37.0530560626126 38.0 36.0 39.0 33.0 40.0 5 36.72410131596216 38.0 36.0 39.0 32.0 40.0 6 37.21809418137132 38.0 36.0 40.0 33.0 40.0 7 37.70022787739957 38.0 38.0 40.0 35.0 40.0 8 37.69323645561163 38.0 38.0 40.0 35.0 40.0 9 37.61263228223392 38.0 38.0 40.0 34.0 40.0 10 36.71443740529964 38.0 36.0 39.0 32.0 40.0 11 37.13035541715881 39.0 36.0 40.0 33.0 40.0 12 36.97122302158273 39.0 36.0 40.0 32.0 40.0 13 36.932507725163155 39.0 36.0 40.0 32.0 40.0 14 37.006705083694236 39.0 36.0 40.0 32.0 40.0 15 37.117601441235074 39.0 36.0 40.0 33.0 40.0 16 36.92313015259434 38.0 36.0 40.0 33.0 40.0 17 36.56675853347173 38.0 36.0 39.0 31.0 40.0 18 36.5015092403689 38.0 35.0 40.0 31.0 40.0 19 36.31561616378539 38.0 35.0 39.0 31.0 40.0 20 36.28107663123233 38.0 35.0 39.0 31.0 40.0 21 35.92364317501223 38.0 35.0 39.0 30.0 40.0 22 35.93662383526015 38.0 35.0 39.0 30.0 40.0 23 35.716095780092346 38.0 35.0 39.0 29.0 40.0 24 35.537742939976376 38.0 35.0 39.0 29.0 40.0 25 35.36384027106673 38.0 35.0 39.0 29.0 40.0 26 34.86138850113939 38.0 34.0 39.0 28.0 40.0 27 34.718648961427874 38.0 33.0 39.0 27.0 40.0 28 34.23698056480189 37.0 33.0 39.0 26.0 40.0 29 34.15468222436976 37.0 33.0 39.0 26.0 40.0 30 33.874667430234915 37.0 33.0 39.0 25.0 40.0 31 33.104429888924685 36.0 32.0 39.0 23.0 40.0 32 33.10506221888161 36.0 32.0 39.0 23.0 40.0 33 33.0288724244485 36.0 32.0 39.0 22.0 40.0 34 32.655272796688024 36.0 31.0 39.0 21.0 40.0 35 31.885297731963682 35.0 30.0 39.0 17.0 40.0 36 32.51805719603422 36.0 32.0 39.0 17.0 40.0 37 32.03090065261224 36.0 31.0 39.0 13.0 40.0 38 31.693737547275614 36.0 31.0 39.0 10.0 40.0 39 31.630826681938032 36.0 31.0 39.0 8.0 40.0 40 31.39636350620996 36.0 30.0 39.0 2.0 40.0 41 30.9980791486214 36.0 30.0 39.0 2.0 40.0 42 30.793144588806566 36.0 30.0 39.0 2.0 40.0 43 30.416371380507535 35.0 29.0 39.0 2.0 40.0 44 30.461767899113546 35.0 30.0 39.0 2.0 40.0 45 29.966438789267094 35.0 29.0 39.0 2.0 40.0 46 28.972058174356036 35.0 27.0 38.0 2.0 40.0 47 28.393356956226064 34.0 25.0 38.0 2.0 40.0 48 28.64594294713483 34.0 27.0 38.0 2.0 39.0 49 28.332152188696803 34.0 26.0 38.0 2.0 40.0 50 27.968252263860553 34.0 25.0 38.0 2.0 39.0 51 27.309233210446568 34.0 23.0 38.0 2.0 39.0 52 26.860648794397317 33.0 20.0 38.0 2.0 39.0 53 26.61108128422635 33.0 17.0 38.0 2.0 39.0 54 26.23318658506031 33.0 7.0 37.0 2.0 39.0 55 25.788539317799493 33.0 2.0 38.0 2.0 39.0 56 15.056515981244855 19.0 2.0 23.0 2.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.9739665135095805 1 2 -0.9269653283032184 1 3 -2.9810363108507687 1 4 -1.213988371782257 1 5 -1.7125487294218047 1 6 -0.803587066922617 1 7 -1.6947688195445707 1 8 -1.7208466038347723 1 9 -2.133262738510169 1 10 -1.7956476138500648 1 11 -0.9708448587409464 1 12 -0.9994696808691614 1 13 -1.057834012667584 1 14 -0.7100344527704934 1 15 -0.9466905142357902 1 16 -1.9756076886070488 1 17 -1.177081697005704 1 18 -1.5461627067445818 1 19 -1.7949532676956323 1 20 -1.408947033175096 1 21 -1.656281012494894 1 22 -1.0485707634173735 1 23 -2.187936662827113 1 24 -2.163038688035755 1 25 -1.548659438770791 1 26 -1.4410967584573342 1 27 -1.65216419177127 1 28 -1.9242605009277725 1 29 -1.9876541011020734 1 30 -1.4617135324303234 1 31 -1.0935627520247841 1 32 0.22267068906952403 1 33 -1.929979950468418 1 34 0.022996072394491307 1 35 -1.4554515432500068 1 36 -0.3319664653141885 1 37 -1.943513511846028 1 38 -1.8528927490486709 1 39 -1.0428697214173503 1 40 -0.7317472737607389 1 41 -1.806828596529428 1 42 -2.0162200319087518 1 43 -0.8814159024165491 1 44 -1.2190292129695806 1 45 -1.323335615604563 1 46 -0.8493732010356005 1 47 -2.2832948844590533 1 48 -2.4311537433815573 1 49 -1.3806009638076446 1 50 -3.0463638479271786 1 51 -3.4206261521556023 1 52 -3.149468684553728 1 53 -2.5247633077196845 1 54 -3.121575025216373 1 55 -3.2532757886373957 1 56 -2.1309547786273537 2 1 -0.7210527605958248 2 2 -0.804121505459392 2 3 -2.6581924880069465 2 4 -1.2533823111761961 2 5 -1.087607004480084 2 6 -0.884006647342197 2 7 -0.4362606610364139 2 8 -0.4621053450935122 2 9 -0.47288977813720834 2 10 -0.4301464483488999 2 11 -0.9142015020975904 2 12 -1.9386305200299958 2 13 -1.4046871595207264 2 14 -0.15199249472853893 2 15 -1.0557814233266996 2 16 -2.2021811151804727 2 17 -1.454937174861179 2 18 -1.5447641053459833 2 19 -2.6807341534765143 2 20 -1.7863363105643728 2 21 -2.4476563038701897 2 22 -1.8623236771702878 2 23 -1.5895683644588132 2 24 -1.591943116940186 2 25 -2.499242189353538 2 26 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0.19885788065543153 3 11 0.19618810829202005 3 12 0.11591493451545887 3 13 0.07842972359615885 3 14 0.5921633494273024 3 15 0.3675952000499194 3 16 0.284831871832516 3 17 0.09874247881847253 3 18 0.46043069984882123 3 19 -0.01363458637695203 3 20 0.21193208770402805 3 21 1.0129497567358712 3 22 1.086594071747463 3 23 1.375799600909147 3 24 1.657840432843365 3 25 0.5414504513391023 3 26 1.4072548898943111 3 27 0.6884951488880731 3 28 0.8471680705007927 3 29 0.23432392087595133 3 30 -0.11006518078197303 3 31 -0.31004626850830164 3 32 0.0666267330255721 3 33 0.04474532425685851 3 34 0.4196993690977848 3 35 -0.2246823124807733 3 36 1.3515500182022961 3 37 1.1905524222199055 3 38 1.2163380201821 3 39 0.7933940148463847 3 40 1.5968241548106903 3 41 0.6799845902837589 3 42 0.981582165893446 3 43 1.7196829986823516 3 44 0.25899276505239754 3 45 0.8283127360437881 3 46 0.9176597659973638 3 47 2.0463754452112752 3 48 1.467747355519542 3 49 1.3468715636648803 3 50 2.0712185696552368 3 51 2.4903628588334072 3 52 3.017564282479242 3 53 3.180731197774822 3 54 2.9179854143440664 3 55 2.757713222351615 3 56 2.654759507086931 4 1 -0.8488230003063677 4 2 -0.8341983937452326 4 3 -0.7330454956957766 4 4 -1.1721973270061383 4 5 -0.8246038384918393 4 6 -1.2456077328238777 4 7 -0.334722895319544 4 8 -0.43496830303109846 4 9 -0.27999063747687813 4 10 -0.6427199445044849 4 11 -0.7398460068465766 4 12 -0.5810999908576804 4 13 -0.6068581228857184 4 14 -0.550907013046043 4 15 -0.042212902295496235 4 16 -1.138409066333793 4 17 -1.7394238324821671 4 18 -1.618034118914501 4 19 -2.56992456505499 4 20 -1.6151468035539693 4 21 -1.7974980044581557 4 22 -1.0249197875275868 4 23 -1.65085285111644 4 24 -1.8289399509519413 4 25 -1.5110015742472527 4 26 -1.435815472579037 4 27 -1.5575602882121373 4 28 -2.169036620330761 4 29 -2.572499106842603 4 30 -1.052069445174297 4 31 -1.8795558633910296 4 32 -1.1261238000234712 4 33 -1.4659156565533777 4 34 -0.2589786692817455 4 35 -1.1978166408389832 4 36 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0.7654737928012167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 83.0 3 14.0 4 16.0 5 29.0 6 50.0 7 97.0 8 101.0 9 132.0 10 165.0 11 172.0 12 215.0 13 318.0 14 362.0 15 446.0 16 549.0 17 627.0 18 707.0 19 763.0 20 886.0 21 899.0 22 960.0 23 1190.0 24 1343.0 25 1585.0 26 1487.0 27 1654.0 28 1817.0 29 2145.0 30 2552.0 31 3072.0 32 3737.0 33 4759.0 34 5950.0 35 7824.0 36 9719.0 37 11896.0 38 10608.0 39 4888.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 63.52052686209242 23.65152653996206 4.434661226242886 8.393285371702639 11 37.486428767433814 20.57816433420428 13.84325375520479 28.092153143157116 12 40.54905329467769 10.151878497202238 25.080830857701898 24.218237350418175 13 23.238722454871922 14.92895236049966 32.83582089552239 28.996504289106028 14 18.34472720331198 20.87166088025102 41.726618705035975 19.05699321140103 15 26.760681007432858 13.700084708352723 42.294522590882515 17.2447116933319 16 27.066108307383946 14.78936253981889 36.03087679110443 22.113652361692733 17 28.405931970841237 15.793932018564252 22.648150136607132 33.15198587398738 18 30.057148311201786 17.168354868344128 23.62647195676295 29.148024863691134 19 26.049608074734245 22.75910614791749 29.65269575384469 21.538590023503584 20 33.49797773721321 21.672214467232184 25.660665497452783 19.169142298101818 21 25.537778732238092 20.21069711395063 31.025925528234126 23.225598625577152 22 31.407709653172983 18.47357934547884 27.593447629955737 22.525263371392438 23 38.421799873534006 20.832289392366704 23.818557094622808 16.92735363947648 24 35.03943114165384 21.116241335289978 24.16932125941038 19.6750062636458 25 25.714081094307645 25.84657253010898 21.568650855226252 26.87069552035713 26 30.047996561440378 16.494340703949568 27.774726586751996 25.682936147858065 27 19.473413850362565 15.526406957435878 27.497640636012854 37.5025385561887 28 22.01565207001613 30.774837206523685 25.068403130414 22.14110759304618 29 33.64088344966058 17.094129457883163 27.85997705325557 21.40501003920069 30 20.944136215802565 19.658743064855557 33.734694853644534 25.66242586569734 31 30.03157441515572 17.241544275941255 32.36138353346409 20.365497775438932 32 35.95520405843573 13.595520405843573 32.48543294368203 17.96384259203867 33 39.5333524876694 11.881674088971891 29.88315855001676 18.701814873341952 34 28.551758818035967 14.017863556907162 39.48181317497186 17.94856445008501 35 20.176993269944674 23.39951620242857 43.84595118913611 12.577539338490649 36 25.00059904631826 29.026190305034383 29.954712098339442 16.01849855030791 37 30.48688420472384 21.490970532900334 32.29876930820022 15.723375954175603 38 25.905325869364564 25.435431475732113 32.24170792227563 16.417534732627693 39 30.626947974106926 18.687365140254137 32.45624550467514 18.2294413809638 40 25.8536117340881 22.984852664276033 30.635276621772345 20.526258979863517 41 27.150721404670243 19.164297964715335 34.671799853680184 19.01318077693424 42 24.21685590148587 18.404186370292134 34.73199068628628 22.646967041935717 43 22.257828346475595 17.61783026680589 33.72340042487308 26.400940961845436 44 22.07525655644242 13.495769069195223 35.61783592390326 28.8111384504591 45 24.71571464594916 16.040058117892865 32.638480289148525 26.60574694700945 46 25.481248423858876 22.55713136310689 33.2268562439206 18.734763969113637 47 20.252008984877058 19.164934955736268 37.59354241991087 22.989513639475803 48 26.03225341280523 16.69751009421265 35.53162853297443 21.73860796000769 49 27.64166126204031 17.420912300929594 34.855035910739595 20.082390526290506 50 18.297826322050394 17.158718142821094 35.39886810136621 29.144587433762304 51 19.173045265017244 15.92386537051946 39.058651061631075 25.844438302832216 52 16.95235118761419 19.598038272910856 43.27098759496105 20.178622944513897 53 14.618547281863863 18.13853958789161 45.7731239931716 21.469789137072926 54 16.347911855273285 17.290944957659736 42.91883179368745 23.442311393379523 55 18.033693678527193 16.037565685838313 40.301343177691464 25.627397457943026 56 16.700738353495755 20.185670651049808 29.67002573414464 33.44356526130979 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 8.0 11 16.0 12 37.5 13 75.5 14 92.0 15 125.5 16 159.0 17 367.5 18 576.0 19 701.0 20 826.0 21 823.0 22 968.5 23 1117.0 24 1301.5 25 1486.0 26 1917.0 27 2348.0 28 2449.5 29 2554.0 30 2557.0 31 2509.5 32 2462.0 33 1995.0 34 1528.0 35 1873.5 36 2219.0 37 1879.0 38 1249.0 39 959.0 40 909.5 41 860.0 42 935.5 43 1011.0 44 1049.5 45 1088.0 46 1765.5 47 2164.0 48 1885.0 49 1938.0 50 1991.0 51 1897.0 52 1803.0 53 2453.5 54 3390.0 55 3676.0 56 3671.0 57 3666.0 58 4639.0 59 5612.0 60 6005.5 61 6399.0 62 7315.0 63 8333.5 64 8436.0 65 6471.5 66 4507.0 67 3681.0 68 2855.0 69 2225.0 70 1595.0 71 1289.5 72 905.5 73 827.0 74 646.5 75 466.0 76 403.5 77 341.0 78 279.0 79 225.0 80 233.0 81 195.5 82 158.0 83 113.5 84 69.0 85 47.5 86 26.0 87 18.5 88 7.5 89 4.0 90 3.0 91 2.0 92 2.5 93 3.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.045336864836489014 26 0.07277759881646921 27 0.12885214216686353 28 0.14555519763293842 29 0.17299593161291862 30 0.22071894723027546 31 0.24458045503895393 32 0.2839519429232733 33 0.34002648627366766 34 0.35195724017800684 35 0.37104644642494955 36 0.4187694620423064 37 0.43905174367968314 38 0.4700717038309651 39 0.4736509300022668 40 0.5201808702291898 41 0.5225670210100577 42 0.5953446198265269 43 0.5941515444360929 44 0.5989238459978287 45 0.6418745600534498 46 0.6490330123960533 47 0.6752806709855996 48 0.7170383096507869 49 0.6621568416908264 50 0.7086867819177495 51 0.7110729326986173 52 0.745672119021201 53 0.7576028729255402 54 0.8124843408855005 55 0.7826574561246527 56 0.7862366822959543 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 83817.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.108450552990444 #Duplication Level Percentage of deduplicated Percentage of total 1 70.04675859882707 21.08999367670043 2 10.964495165636393 6.602479210661322 3 4.933428435568236 4.456136583270697 4 3.2255508004438105 3.8846534712528484 5 2.0922491678554445 3.149719030745553 6 1.4186083372959264 2.5627259386520636 7 1.1531145981930575 2.430294570313898 8 0.9510223490252021 2.290704749633129 9 0.7330797273735933 1.9864705250724795 >10 3.9942938659058487 21.84998270040684 >50 0.23379299413536217 4.981089755061623 >100 0.21398002853067047 10.8188076404548 >500 0.02377555872563005 5.396279990932627 >1k 0.01585037248375337 8.500662156841692 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 3169 3.780855912285097 No Hit GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1879 2.2417886586253384 No Hit GGTACAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1055 1.2586945369077873 No Hit GGTACAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 1022 1.2193230490234679 No Hit GGTACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 979 1.1680208072348093 No Hit GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 928 1.1071739623226793 No Hit GGTACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 836 0.9974110264027584 No Hit GGTACAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC 710 0.8470835272080843 No Hit GGTACAGGGGTTTTTAGAAGTTATTAAACCTTTCTGTGCAGTTCTACCAGAAATTC 549 0.6549983893482229 No Hit GGTACAGGGATTGAACACCCGCACGTGCTCAAGCTCCACGACGTCTACGAGAACAA 521 0.6215922784160731 No Hit GGTACAGGGGGCGCATGAGCTCTGACTTCTACAGGCCTTCCACACGAGGCGACATG 439 0.5237600964004915 No Hit GGTACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC 435 0.5189877948387558 No Hit GGTACAGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC 379 0.4521755729744562 No Hit GGTACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 322 0.38417027571972273 No Hit GGTACAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 263 0.31377882768412135 No Hit GGTACAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG 247 0.2946896214371786 No Hit GGTACAGGGCGCGGTTCCAGCTTTATGTAAACACACACACACCCCAGTTATAGTTC 243 0.28991731987544295 No Hit GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 234 0.2791796413615376 No Hit GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 232 0.2767934905806698 No Hit GGTACAGGGCGGATTTACCAGGCTTTTCTCATGTCGGCCACTTTCAATGAGGATGT 226 0.2696350382380662 No Hit GGTACAGGGCTGTGGTGACTTTTGTTTGCAACTGTAATAAGTTATAAACTGCATGA 226 0.2696350382380662 No Hit GGTACAGGGGGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGC 203 0.24219430425808608 No Hit GGTACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC 200 0.2386150780867843 No Hit GGTACAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 172 0.2052089671546345 No Hit GGTACAGGGGTTCTATTTTTCTAATAGTCTTCTTACAGTTTCTTATAATGATGACA 171 0.20401589176420057 No Hit GGTACAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC 168 0.20043666559289883 No Hit GGTACAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA 164 0.19566436403116316 No Hit GGTACAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 163 0.1944712886407292 No Hit GGTACAGGGAGATAGAGTCTTAAGAGAGATGATATCGTACTTTTTGTAGAACGGGC 161 0.19208513785986137 No Hit GGTACAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 161 0.19208513785986137 No Hit GGTACAGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC 161 0.19208513785986137 No Hit GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 154 0.18373361012682393 No Hit GGTACAGGGACCGTGGTATCTTTATTGATGACATGTGTAGAGAAAGCAGGAACCGA 151 0.18015438395552213 No Hit GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC 150 0.1789613085650882 No Hit GGTACAGGGCACACCTTACACTGCACTGTAGCTTGGTCTCTAACCACAGTTAGAAG 148 0.1765751577842204 No Hit GGTACAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA 146 0.17418900700335255 No Hit GGTACAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 145 0.17299593161291862 No Hit GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC 143 0.17060978083205078 No Hit GGTACAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 143 0.17060978083205078 No Hit GGTACAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 141 0.16822363005118293 No Hit GGTACAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG 139 0.16583747927031509 No Hit GGTACAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG 138 0.16464440387988116 No Hit GGTACAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG 131 0.15629287614684373 No Hit GGTACAGGGATCTACGTCTGTCTGTGGAGATAATTGTAGCTGTACTCCGTCTCTAC 130 0.1550998007564098 No Hit GGTACAGGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT 130 0.1550998007564098 No Hit GGTACAGGGATCTTTCATGTGTCCCCTTTGGGCCTGGGTAGGTCTTTCTTTGGTGT 129 0.15390672536597588 No Hit GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC 120 0.14316904685207058 No Hit GGTACAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG 118 0.14078289607120273 No Hit GGTACAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATC 116 0.1383967452903349 No Hit GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCG 116 0.1383967452903349 No Hit GGTACAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 115 0.137203669899901 No Hit GGTACAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC 115 0.137203669899901 No Hit GGTACAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 115 0.137203669899901 No Hit GGTACAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 114 0.13601059450946706 No Hit GGTACAGGGGGGAATCCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 112 0.1336244437285992 No Hit GGTACAGGGAGCTAGAGTCAAGAGCTCCGGGGTACTGGTTAGCTCATAATGTTGTT 109 0.13004521755729745 No Hit GGTACAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 107 0.1276590667764296 No Hit GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 106 0.12646599138599568 No Hit GGTACAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA 105 0.12527291599556176 No Hit GGTACAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 104 0.12407984060512783 No Hit GGTACAGGGATTGTGAACTTCGTTCACACCAACTTGAGGAAAAACAACAGACAGCC 103 0.12288676521469391 No Hit GGTACAGGGGGGGGGATGTTGGTTGTGTTTGGATATACGACTGCTATAGCTACTGA 103 0.12288676521469391 No Hit GGTACAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT 101 0.12050061443382608 No Hit GGTACAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 101 0.12050061443382608 No Hit GGTACAGGGGGGGCTGGTGAGATAGCACAGTGGGTAAGAGCACCCAACTGCTCTTC 99 0.11811446365295823 No Hit GGTACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTC 98 0.11692138826252432 No Hit GGTACAGGGGGGAGCTGGAGAGATGGCTCAGCGGTTAAGAGCACCAACTGCTCTTC 97 0.11572831287209037 No Hit GGTACAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC 95 0.11334216209122254 No Hit GGTACAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA 95 0.11334216209122254 No Hit GGTACAGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTT 94 0.11214908670078862 No Hit GGTACAGGGGTCCTACCGTCCCCTCACTAGAGCTTCTTGTACCCTACTCCATCTCT 93 0.11095601131035471 No Hit GGTACAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGA 93 0.11095601131035471 No Hit GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC 92 0.10976293591992078 No Hit GGTACAGGGGGGCTGGTGAGATGGCTCAGCTGGTAAGAGCACTGACTGCTCTTCCA 89 0.106183709748619 No Hit GGTACAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCG 89 0.106183709748619 No Hit GGTACAGGGCGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT 88 0.10499063435818509 No Hit GGTACAGGGGGGGCTGGAGAGATGGCTCAATGGTTAAGAGCACTGAGCACTGACTG 88 0.10499063435818509 No Hit GGTACAGGGATTTCCATTCTAGAACCATCCTTTGTTCTTCTTGTTTCAGTTTTTAC 88 0.10499063435818509 No Hit GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC 84 0.10021833279644941 No Hit GGTACAGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCC 84 0.10021833279644941 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.004772301561735686 0.0 0.0 0.0 0.0 12 0.005965376952169608 0.0 0.0 0.0 0.0 13 0.005965376952169608 0.0 0.0 0.0 0.0 14 0.008351527733037451 0.0 0.0 0.0 0.0 15 0.008351527733037451 0.0 0.0 0.0 0.0 16 0.009544603123471372 0.0 0.0 0.0 0.0 17 0.010737678513905294 0.0 0.0 0.0 0.0 18 0.010737678513905294 0.0 0.0 0.0 0.0 19 0.010737678513905294 0.0 0.0 0.0 0.0 20 0.013123829294773137 0.0 0.0 0.0 0.0 21 0.013123829294773137 0.0 0.0 0.0 0.0 22 0.01550998007564098 0.0 0.0 0.0 0.0 23 0.016703055466074902 0.0 0.0 0.0 0.0 24 0.019089206246942745 0.0 0.0 0.0 0.0 25 0.019089206246942745 0.0 0.0 0.0 0.0 26 0.019089206246942745 0.0 0.0 0.0 0.0 27 0.019089206246942745 0.0 0.0 0.0 0.0 28 0.04056456327475333 0.0 0.0 0.0 0.0 29 0.04056456327475333 0.0 0.0 0.0 0.0 30 0.04652994022692294 0.0 0.0 0.0 0.0 31 0.04772301561735686 0.0 0.0 0.0 0.0 32 0.048916091007790784 0.0 0.0 0.0 0.0 33 0.048916091007790784 0.0 0.0 0.0 0.0 34 0.048916091007790784 0.0 0.0 0.0 0.0 35 0.05965376952169608 0.0 0.0 0.0 0.0 36 0.05965376952169608 0.0 0.0 0.0 0.0 37 0.06084684491213 0.0 0.0 0.0 0.0 38 0.06203992030256392 0.0 0.0 0.0 0.0 39 0.06323299569299784 0.0 0.0 0.0 0.0 40 0.06800529725473353 0.0 0.0 0.0 0.0 41 0.07277759881646921 0.0 0.0 0.0 0.0 42 0.07397067420690313 0.0 0.0 0.0 0.0 43 0.07635682498777098 0.0 0.0 0.0 0.0 44 0.0775499003782049 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCATGA 25 1.990749E-5 50.333534 50 GAAATTC 55 0.0 50.333534 50 TCGCCGT 15 0.008185656 50.333534 50 GAACCGA 30 9.896157E-7 50.333534 50 TATCACT 20 4.0270033E-4 50.333534 50 CGACTGA 15 0.008185656 50.333534 50 CAAGCCC 20 4.0270033E-4 50.333534 50 ATGATGA 35 4.96766E-8 50.3032 48 ACTGCAT 25 1.9978725E-5 50.303196 48 GCAAGCC 20 4.0390156E-4 50.303196 49 ACTCTTC 20 4.0390156E-4 50.303196 49 AGAAATT 50 5.456968E-12 50.303196 49 GTTAGAA 15 0.008205204 50.303196 49 CAGACAG 15 0.008205204 50.303196 48 TCTCCCC 15 0.008205204 50.303196 48 ACGGCCT 15 0.008205204 50.303196 49 GGAACCG 30 9.937467E-7 50.303196 49 ACTATCA 20 4.0390156E-4 50.303196 48 AGTTAGA 15 0.008205204 50.303196 48 TATAATG 35 4.9914888E-8 50.272896 44 >>END_MODULE