FastQCFastQC Report
Tue 31 May 2016
SRR212538_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212538_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79419
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC32364.074591722383812No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC17332.18209748297007No Hit
GGTACAGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGAC10541.3271383422103025No Hit
GGTACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC10191.2830682834082525No Hit
GGTACAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9781.231443357382994No Hit
GGTACAGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA9761.228925068308591No Hit
GGTACAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA8431.0614588448608016No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC8221.0350168095795718No Hit
GGTACAGGGGTTTTTAGAAGTTATTAAACCTTTCTGTGCAGTTCTACCAGAAATTC4920.6194991123031013No Hit
GGTACAGGGATTGAACACCCGCACGTGCTCAAGCTCCACGACGTCTACGAGAACAA4790.6031302333194827No Hit
GGTACAGGGGGCGCATGAGCTCTGACTTCTACAGGCCTTCCACACGAGGCGACATG4190.5275815610873973No Hit
GGTACAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC4140.5212858384013901No Hit
GGTACAGGGGCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC3470.4369231544088946No Hit
GGTACAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA3050.38403908384643476No Hit
GGTACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2640.3324141578211763No Hit
GGTACAGGGCGCGGTTCCAGCTTTATGTAAACACACACACACCCCAGTTATAGTTC2410.3034538334655435No Hit
GGTACAGGGGGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGC2320.29212153263073065No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2310.29086238809352927No Hit
GGTACAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCG2260.2845666654075221No Hit
GGTACAGGGCGGATTTACCAGGCTTTTCTCATGTCGGCCACTTTCAATGAGGATGT2230.28078923179591786No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2150.27071607549830645No Hit
GGTACAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCC2090.2631612082750979No Hit
GGTACAGGGGTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACT2070.26064291920069504No Hit
GGTACAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1970.2480514738286808No Hit
GGTACAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA1840.23168259484506226No Hit
GGTACAGGGCTGTGGTGACTTTTGTTTGCAACTGTAATAAGTTATAAACTGCATGA1830.23042345030786082No Hit
GGTACAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1690.21279542678704091No Hit
GGTACAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1680.21153628224983945No Hit
GGTACAGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC1520.19138996965461666No Hit
GGTACAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC1520.19138996965461666No Hit
GGTACAGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1480.18635339150581096No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1470.18509424696860954No Hit
GGTACAGGGAGATAGAGTCTTAAGAGAGATGATATCGTACTTTTTGTAGAACGGGC1460.1838351024314081No Hit
GGTACAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1450.18257595789420666No Hit
GGTACAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.18131681335700525No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC1400.17628023520819955No Hit
GGTACAGGGGTTCTATTTTTCTAATAGTCTTCTTACAGTTTCTTATAATGATGACA1390.17502109067099814No Hit
GGTACAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAG1390.17502109067099814No Hit
GGTACAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1340.168725367984991No Hit
GGTACAGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG1330.16746622344778958No Hit
GGTACAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG1310.16494793437338673No Hit
GGTACAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGA1230.15487477807577532No Hit
GGTACAGGGGGGAATCCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1200.15109734446417106No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGTTCTTCC1180.14857905538976818No Hit
GGTACAGGGACCGTGGTATCTTTATTGATGACATGTGTAGAGAAAGCAGGAACCGA1160.14606076631536533No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC1140.1435424772409625No Hit
GGTACAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.1397650436293582No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCG1110.1397650436293582No Hit
GGTACAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1110.1397650436293582No Hit
GGTACAGGGCACACCTTACACTGCACTGTAGCTTGGTCTCTAACCACAGTTAGAAG1090.13724675455495536No Hit
GGTACAGGGATCTTTCATGTGTCCCCTTTGGGCCTGGGTAGGTCTTTCTTTGGTGT1070.1347284654805525No Hit
GGTACAGGGCGGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1050.13221017640614965No Hit
GGTACAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAA1050.13221017640614965No Hit
GGTACAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.13095103186894824No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC1040.13095103186894824No Hit
GGTACAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAACCCCG1020.1284327427945454No Hit
GGTACAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATC980.1233961646457397No Hit
GGTACAGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT970.12213702010853826No Hit
GGTACAGGGATCTACGTCTGTCTGTGGAGATAATTGTAGCTGTACTCCGTCTCTAC960.12087787557133683No Hit
GGTACAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC960.12087787557133683No Hit
GGTACAGGGCTCTTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCG920.11584129742253113No Hit
GGTACAGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAA920.11584129742253113No Hit
GGTACAGGGAGCTAGAGTCAAGAGCTCCGGGGTACTGGTTAGCTCATAATGTTGTT910.1145821528853297No Hit
GGTACAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT890.11206386381092685No Hit
GGTACAGGGGGGGCTGGTGAGATAGCACAGTGGGTAAGAGCACCCAACTGCTCTTC870.109545574736524No Hit
GGTACAGGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTT870.109545574736524No Hit
GGTACAGGGATTGTGAACTTCGTTCACACCAACTTGAGGAAAAACAACAGACAGCC870.109545574736524No Hit
GGTACAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT850.10702728566212116No Hit
GGTACAGGGGTCCTACCGTCCCCTCACTAGAGCTTCTTGTACCCTACTCCATCTCT840.10576814112491972No Hit
GGTACAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT840.10576814112491972No Hit
GGTACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGGGCACCCGACTGCTCTTC840.10576814112491972No Hit
GGTACAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC840.10576814112491972No Hit
GGTACAGGGGGGGCTGGAGAGATGGCTCAATGGTTAAGAGCACTGAGCACTGACTG820.10324985205051689No Hit
GGTACAGGGATTTCCATTCTAGAACCATCCTTTGTTCTTCTTGTTTCAGTTTTTAC820.10324985205051689No Hit
GGTACAGGGGGGGCTGAAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC800.10073156297611403No Hit
GGTACAGGGGGGGGGATGTTGGTTGTGTTTGGATATACGACTGCTATAGCTACTGA800.10073156297611403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGTT301.003229E-650.2292150
ACATGCA150.00825153450.2292150
AGGATGT150.00825153450.2292150
AGATTTC150.00825153450.2292150
CAAGCCC150.00825153450.2292150
GCGACTG252.0144025E-550.22920650
GGACGCG204.067189E-450.22920650
ATCCCCT150.00827227950.1973448
TAGAACG252.0219915E-550.1973447
CCAACTA150.00827227950.1973443
GTAGAAC252.0219915E-550.1973446
CCTACTC150.00827227950.1973443
CAATGAG150.00827227950.1973445
CAGCATT252.0219915E-550.1973443
ACGCCAA150.00827227950.1973444
AGCTCAT301.0076383E-650.1973441
GAGGATG150.00827227950.1973449
ATGATGA301.0076383E-650.1973448
GTACCCT150.00827227950.1973439
TATAATG301.0076383E-650.1973444
Produced by FastQC (version 0.11.5)