FastQCFastQC Report
Tue 31 May 2016
SRR212537_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212537_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155058
Sequences flagged as poor quality0
Sequence length58
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT24971.6103651536844277No Hit
GAGGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC23991.5471629970720633No Hit
GAGGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG16681.0757265023410594No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA16381.0563789033780908No Hit
GAGGAGGGGGACACATTTTTAAAAAATATAAATTGTATGTTCATTATAAAGAAATTGT9870.6365360058816701No Hit
GAGGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT8660.558500690064363No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG6360.41016909801493634No Hit
GAGGAGGGGACACCTATGTCAACACACCAACTGGGGATGAGGATGGCAGAAGTCGCCA5840.37663325981245727No Hit
GAGGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.35277122109146253No Hit
GAGGAGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT5170.33342362212849386No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA4470.28827922454823357No Hit
GAGGAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG4360.28118510492847837No Hit
GAGGAGGGGAACTATCTGTAAACCAACCTTTCAGTTGCTATATAACTTCCATAGACAT3780.24377974693340557No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA3510.22636690786673372No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA3000.19347598962968696No Hit
GAGGAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA3000.19347598962968696No Hit
GAGGAGGGGACGATACCTTTTTCTTGGTGATTATGTGGACAGAGGTTATTTTAGTATA2830.18251235021733803No Hit
GAGGAGGGGGCTCATTATATTCAAATATTCATTTTAGGAGCCATTCCGTAGTGCCATT2750.1773529904938797No Hit
GAGGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.17412839066671826No Hit
GAGGAGGGGGCATCCTTCCGGCTCCGTCGTGGAAGGAGGACTGCGCCTCGTCTTCCTG2690.17348347070128597No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGATCTTCCAAA2670.1721936307704214No Hit
GAGGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC2660.1715487108049891No Hit
GAGGAGGGGGTATCAGGTCGACCCTGATGCCTGCTTTTCGGCCAAAGTGAACAACTCT2650.1709037908395568No Hit
GAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA2610.16832411097782765No Hit
GAGGAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2550.16445459118523392No Hit
GAGGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA2480.15994015142720788No Hit
GAGGAGGGGGACTTCCTGTCAGCGAGCTGAATCCCCTGGAGATGGGGGGGTGGGCTGA2420.15607063163461413No Hit
GAGGAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT2390.15413587173831728No Hit
GAGGAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2290.14768667208399439No Hit
GAGGAGGGGGGTCACAACTGAAATCAGCATCCTGATTACTTTGTGTTCCAGTGTTCTG2290.14768667208399439No Hit
GAGGAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG2280.14704175211856207No Hit
GAGGAGGGGAACTTCTTAAAAGACAAAAAAGAGAGAAATTGTATCCCCGTGCATGCTA2240.14446207225683294No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGCTCTTCCGA2240.14446207225683294No Hit
GAGGAGGGGATTGCAGATGGTGGTTTTACTGAGTTGCACTCACTGTGGCAGAATGAAG2100.13543319274078086No Hit
GAGGAGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG2090.13478827277534858No Hit
GAGGAGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG2030.13091875298275485No Hit
GAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.1276941531555934No Hit
GAGGAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1970.1270492331901611No Hit
GAGGAGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1950.12575939325929653No Hit
GAGGAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA1940.12511447329386424No Hit
GAGGAGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC1930.12446955332843193No Hit
GAGGAGGGGCCACCTTCCATGAGCAGCTTGCCCAGGCACTCCAGGTCTGAGAAGCAAG1900.12253479343213507No Hit
GAGGAGGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA1850.11931019360497362No Hit
GAGGAGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC1800.11608559377781218No Hit
GAGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG1790.11544067381237988No Hit
GAGGAGGGGGAACTTGTCAAGACATGGTCGATCTTCCTCAAGCTAGAAGCATGGAGTC1780.11479575384694758No Hit
GAGGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTAAAAAACATCGAAC1750.11286099395065073No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG1740.11221607398521843No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA1720.11092623405435387No Hit
GAGGAGGGGGAGCCCAAGGGTGAATGGCCTGACCACAGTAGCCTTGAAAGACACCAGC1720.11092623405435387No Hit
GAGGAGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA1690.10899147415805699No Hit
GAGGAGGGGCAATTGGGTTGTGCCTTTGCGTTCGAATGCTGACCCATGCAATATGGCC1660.10705671426176012No Hit
GAGGAGGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGGGC1640.10576687433089553No Hit
GAGGAGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1630.10512195436546326No Hit
GAGGAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1610.10383211443459867No Hit
GAGGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCCG1600.10318719446916638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACTC353.8438884E-852.0177551
CCTTACT150.007198695652.0177539
CTCAAGC307.931285E-752.0177537
TACTTCC150.007198695652.0177546
ACTGATC401.87174E-952.0177545
CTATATA700.052.0177538
TAGAATC150.007198695652.0177534
TAGGTGC203.4325678E-452.0177547
AGAAGTC353.8438884E-852.0177548
ACCTGAT150.007198695652.0177540
TATATGG203.4325678E-452.0177551
TCCTCAA307.931285E-752.0177535
TAAAATG203.4325678E-452.0177538
CAATTCT150.007198695652.0177551
TAAGTAC307.931285E-752.0177552
ATATGCT150.007198695652.0177534
TAACGGC150.007198695652.0177537
TTATATG203.4325678E-452.0177550
GCTCTTA150.007198695652.0177549
GTGTGAC307.931285E-752.0177534
Produced by FastQC (version 0.11.5)