##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR212537_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 155058 Sequences flagged as poor quality 0 Sequence length 58 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.55757200531414 38.0 38.0 39.0 34.0 40.0 2 36.0155877155645 38.0 35.0 39.0 30.0 40.0 3 37.21443588850624 38.0 37.0 39.0 33.0 40.0 4 37.049239639360756 38.0 36.0 39.0 33.0 40.0 5 34.905299952275925 37.0 33.0 39.0 27.0 40.0 6 37.40755072295528 38.0 38.0 39.0 33.0 40.0 7 37.58954713720027 39.0 38.0 39.0 35.0 40.0 8 36.56366649898747 39.0 35.0 40.0 31.0 40.0 9 36.509119168311216 39.0 35.0 39.0 31.0 40.0 10 35.456861303512234 38.0 35.0 39.0 28.0 40.0 11 36.307149582736784 38.0 35.0 39.0 31.0 40.0 12 36.51757406905803 38.0 35.0 39.0 31.0 40.0 13 36.46002141134285 38.0 35.0 39.0 31.0 40.0 14 36.47698925563338 38.0 35.0 39.0 31.0 40.0 15 36.579725006126736 38.0 35.0 39.0 31.0 40.0 16 36.295418488565566 38.0 35.0 39.0 31.0 40.0 17 36.04989100852584 38.0 35.0 39.0 30.0 40.0 18 35.95530704639554 38.0 35.0 39.0 30.0 40.0 19 35.67058778005649 38.0 35.0 39.0 29.0 40.0 20 35.718344103496754 38.0 35.0 39.0 29.0 40.0 21 35.52011505372183 38.0 35.0 39.0 29.0 40.0 22 35.385752428123666 38.0 35.0 39.0 29.0 40.0 23 35.221162403745694 38.0 34.0 39.0 28.0 40.0 24 35.101091204581515 38.0 34.0 39.0 28.0 40.0 25 35.10737917424448 38.0 34.0 39.0 28.0 40.0 26 34.84696049220292 38.0 34.0 39.0 28.0 40.0 27 34.67535373860104 38.0 33.0 39.0 27.0 40.0 28 34.18455674650776 37.0 33.0 39.0 26.0 40.0 29 34.294522049813615 38.0 33.0 39.0 26.0 40.0 30 33.825581395348834 37.0 33.0 39.0 25.0 40.0 31 33.237781991254884 37.0 32.0 39.0 22.0 40.0 32 33.13616195230172 37.0 32.0 39.0 22.0 40.0 33 33.17068451805131 36.0 32.0 39.0 23.0 40.0 34 32.589102142424125 36.0 31.0 38.0 20.0 40.0 35 32.12457918972255 36.0 30.0 38.0 18.0 40.0 36 32.88613293090327 37.0 33.0 39.0 19.0 40.0 37 32.49208683202414 36.0 32.0 39.0 17.0 40.0 38 32.323279031072246 36.0 32.0 39.0 16.0 40.0 39 32.402449406028715 36.0 32.0 39.0 16.0 40.0 40 32.064440402946 36.0 31.0 39.0 15.0 40.0 41 32.230578235241005 37.0 32.0 39.0 10.0 40.0 42 32.162745553276835 37.0 32.0 39.0 9.0 40.0 43 31.91499309935637 36.0 31.0 39.0 8.0 40.0 44 31.827258187258963 36.0 31.0 39.0 2.0 40.0 45 31.535122341317443 36.0 31.0 39.0 2.0 40.0 46 31.105334777954056 36.0 30.0 39.0 2.0 40.0 47 30.90658334300713 36.0 30.0 39.0 2.0 40.0 48 30.77988881579796 36.0 30.0 39.0 2.0 40.0 49 30.607353377445857 35.0 29.0 39.0 2.0 40.0 50 30.344226031549486 35.0 29.0 39.0 2.0 40.0 51 29.944020947000478 35.0 29.0 39.0 2.0 39.0 52 29.725083517135523 35.0 28.0 39.0 2.0 39.0 53 29.440183673206157 35.0 28.0 39.0 2.0 39.0 54 29.31936436688207 35.0 28.0 39.0 2.0 39.0 55 28.981387609797626 35.0 27.0 39.0 2.0 39.0 56 28.63987669130261 35.0 27.0 39.0 2.0 39.0 57 28.340633827342028 35.0 26.0 38.0 2.0 39.0 58 27.883385571850532 35.0 24.0 38.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1 1 -1.9743402055806598 1 2 -2.697844896181202 1 3 -2.8583144242640515 1 4 -2.920963785625858 1 5 -3.4213918250842283 1 6 -3.239673207177475 1 7 -4.775885279269417 1 8 -4.770290357862038 1 9 -4.66606611451671 1 10 -4.500782278567645 1 11 -4.035779584423054 1 12 -4.163588881749966 1 13 -4.033770398871788 1 14 -4.0710385436438585 1 15 -3.7579609518707358 1 16 -3.466633790311583 1 17 -3.6343976269720386 1 18 -3.570064858028985 1 19 -3.1755470534620116 1 20 -3.0876344649138474 1 21 -3.2375268695679935 1 22 -3.3751092059350896 1 23 -3.836765564297476 1 24 -4.035687784924988 1 25 -3.586182010293726 1 26 -3.480883078984572 1 27 -3.4708476795718965 1 28 -3.3734804883520972 1 29 -3.178482938274481 1 30 -3.4295633017944844 1 31 -3.1865160715717806 1 32 -3.276315464947995 1 33 -3.2868142451543854 1 34 -3.0541436592017206 1 35 -3.636968784529685 1 36 -2.886648173551375 1 37 -3.8589455530307646 1 38 -3.7425774285659266 1 39 -2.843598573453942 1 40 -2.7893473609502806 1 41 -3.1040654399511425 1 42 -3.153948660111073 1 43 -2.725168410645516 1 44 -3.960506648464591 1 45 -3.078542400026386 1 46 -3.4120694364848134 1 47 -3.2548761531855455 1 48 -3.590197323835312 1 49 -3.319115275520911 1 50 -2.6833504955428005 1 51 -2.869366266165862 1 52 -2.04651456047365 1 53 -2.6747237351585795 1 54 -2.2735166231801536 1 55 -2.6049953379880897 1 56 -2.8398047661230947 1 57 -2.418346252011947 1 58 -2.6672221228041018 2 1 -0.5085300108023745 2 2 -0.5018233462433699 2 3 -0.7594748055321858 2 4 -0.7742584395835905 2 5 -0.35694922251481387 2 6 -0.4412203822016565 2 7 -1.175940535520283 2 8 -1.3154623429428511 2 9 -0.8571146018768445 2 10 -0.8328723462565506 2 11 -0.41345605907424954 2 12 -0.3551899316187317 2 13 -0.5929636576091823 2 14 -0.7256179054341558 2 15 -1.1370879031070942 2 16 -0.7661226699910983 2 17 -0.5121431719368132 2 18 -0.4624533094725578 2 19 -0.47151334714898496 2 20 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0.025520792073059795 117 25 0.24032871095383967 117 26 0.0766217943097729 117 27 -0.041607913490025794 117 28 0.587143293324317 117 29 -0.025851359327113244 117 30 -0.20207792167752814 117 31 0.6263494255042446 117 32 0.4634506169233532 117 33 0.3572403357423042 117 34 0.12265945970666081 117 35 0.4355458353533592 117 36 1.3242680057858536 117 37 1.4888127315696238 117 38 1.455278321921405 117 39 1.3575710171893292 117 40 1.0203992277436775 117 41 0.8663049304192256 117 42 1.0772404237095898 117 43 1.6268783729802188 117 44 1.1295518310675696 117 45 1.538221732527223 117 46 2.2715562945093417 117 47 1.7782622483739061 117 48 2.3819274324999675 117 49 1.4198710792549178 117 50 2.12425184363849 117 51 2.4039280808126904 117 52 1.6451033732495475 117 53 1.7642626196172486 117 54 1.7722923436814462 117 55 1.7445173325772139 117 56 1.8099028362160858 117 57 1.410893514069926 117 58 1.967475732946383 118 1 -0.6256559950543448 118 2 -0.9180461345712985 118 3 -0.36953733448077486 118 4 -0.7541681199911849 118 5 -0.8246425990780324 118 6 -0.4948331658978091 118 7 -0.6626241853581689 118 8 -0.6205225560044525 118 9 -0.8158339163742951 118 10 -1.0295490484334877 118 11 -0.6903203480969466 118 12 -0.2101575092009469 118 13 -0.3094411935054282 118 14 -0.8628342092785317 118 15 -0.031309765080230534 118 16 0.41584814983289675 118 17 -0.10692084678628078 118 18 -0.4989864266564368 118 19 0.17404014984036564 118 20 0.08341816666509771 118 21 0.24621926046296494 118 22 0.03523651129917482 118 23 -0.5332309925740475 118 24 -0.100187268929119 118 25 0.11260539940699488 118 26 0.04775469191326209 118 27 0.18911104075834118 118 28 -0.1465713036255707 118 29 0.48689373871210506 118 30 0.3742292678649548 118 31 0.30445400066763995 118 32 -0.011222585691029963 118 33 -0.7052868973731705 118 34 0.11045902397681573 118 35 0.17443472424224638 118 36 -0.022355087896059445 118 37 0.4529739516131954 118 38 0.16415631756410676 118 39 0.014760559672993168 118 40 0.43962580726437395 118 41 0.006228677695915508 118 42 0.2454321448424892 118 43 0.19730320958152348 118 44 -0.1854808486709878 118 45 0.4795071355773288 118 46 0.3503144644439793 118 47 0.001573795214866891 118 48 -0.41295274179196895 118 49 0.34677739733770707 118 50 -0.09176122825693867 118 51 -0.31170372746617403 118 52 -0.8868138380794299 118 53 -0.6264781211234904 118 54 -1.147097634532063 118 55 -0.41485085914391817 118 56 0.16006623490889638 118 57 -0.5132894924660221 118 58 -0.24423450670503044 119 1 -0.4405056191144965 119 2 -0.5549877533240632 119 3 -0.9071866047151786 119 4 -1.0863773194604462 119 5 -1.3574820506481373 119 6 -0.670750901413065 119 7 -0.6806471840313222 119 8 -0.743974568388353 119 9 -0.944261603238516 119 10 -0.7213336570137585 119 11 -0.3841505117413462 119 12 0.2699950781497833 119 13 -0.1039458374682738 119 14 -0.4131438068017488 119 15 -0.30557999948978676 119 16 -0.74232301336923 119 17 -0.6569540179494808 119 18 -0.31693202594878755 119 19 -0.8610107126098825 119 20 -0.7944013822070843 119 21 -0.29391784701159906 119 22 0.33792337551854246 119 23 0.6695000998629226 119 24 0.1923823905136075 119 25 -0.43079353911578266 119 26 -0.3079507481575021 119 27 -0.3781158499979611 119 28 -0.30330504975560757 119 29 -0.5180819357682154 119 30 -0.28294676044109934 119 31 -0.4145862470103765 119 32 -1.001879374722428 119 33 -0.5073656236004993 119 34 -0.8899832581992122 119 35 -0.24849760658482012 119 36 0.3339032048947388 119 37 0.6598514394504349 119 38 0.795016556396476 119 39 0.2015142085009458 119 40 0.34348471925021684 119 41 0.5801450863646451 119 42 0.5227430692122326 119 43 0.2272125417354367 119 44 0.32270137158553425 119 45 -0.42440706168052245 119 46 -0.8744754515224074 119 47 -1.0543749000527143 119 48 -0.6541070982714032 119 49 -0.9569598870497309 119 50 -0.614428189778387 119 51 -0.6513211533839076 119 52 -0.8460133073408187 119 53 -1.4291097000708604 119 54 -0.693316121927019 119 55 -1.4558725309705451 119 56 -0.8611080242684572 119 57 -0.8268675552700913 119 58 -1.2085755062627506 120 1 -2.241040610438958 120 2 -2.080942062173108 120 3 -2.3446504566527153 120 4 -3.026226943520598 120 5 -2.724529476906092 120 6 -3.1413263785674914 120 7 -4.626425090335545 120 8 -5.153893596728434 120 9 -5.167078260265697 120 10 -3.7546621706054317 120 11 -3.09077283678473 120 12 -2.8911529843140684 120 13 -3.1120430034601796 120 14 -3.0234676934414253 120 15 -2.9136627062567015 120 16 -2.671255922565294 120 17 -2.8054502585509837 120 18 -3.191859729823861 120 19 -2.7592177687116717 120 20 -2.810812602565669 120 21 -2.5705228209849977 120 22 -2.556958058836571 120 23 -2.7645658342030117 120 24 -2.7053908888386218 120 25 -2.764657044031921 120 26 -2.5223810546930707 120 27 -3.423445520327629 120 28 -3.015349584168561 120 29 -2.7348257183014724 120 30 -3.305069374668978 120 31 -4.439889890735074 120 32 -4.197874169401434 120 33 -4.5406941371921725 120 34 -3.498138261091057 120 35 -3.710180660373137 120 36 -3.345318888801039 120 37 -3.5294921117352196 120 38 -3.2623142706711903 120 39 -3.5723435127251975 120 40 -3.44555518821074 120 41 -4.152142363028066 120 42 -3.212147040677877 120 43 -4.199190003088159 120 44 -3.1860464595307185 120 45 -4.043117298811811 120 46 -3.4500249020718563 120 47 -3.1494443043326434 120 48 -3.3756224250498867 120 49 -2.9631773537935153 120 50 -3.3208336264469835 120 51 -1.9893055374209254 120 52 -2.561021982875811 120 53 -2.4630165826619503 120 54 -2.5051293087402087 120 55 -2.3334028953430135 120 56 -1.8274903261770739 120 57 -2.4126107594343473 120 58 -1.488578398107748 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 235.0 3 59.0 4 107.0 5 139.0 6 107.0 7 204.0 8 203.0 9 299.0 10 378.0 11 401.0 12 506.0 13 593.0 14 641.0 15 783.0 16 943.0 17 1051.0 18 1100.0 19 1293.0 20 1353.0 21 1349.0 22 1515.0 23 1701.0 24 1970.0 25 1723.0 26 1972.0 27 2486.0 28 2901.0 29 3791.0 30 4570.0 31 5600.0 32 6827.0 33 8405.0 34 10277.0 35 12898.0 36 16962.0 37 23247.0 38 23198.0 39 13268.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 100.0 0.0 0.0 0.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 53.77407163770977 29.61601465258161 5.127758645152137 11.482155064556489 11 33.87506610429646 26.3920597453856 15.659946600626862 24.072927549691084 12 41.28519650711347 13.200221852468108 24.910033664822194 20.60454797559623 13 20.625830334455493 17.238065756039674 30.76332727108566 31.372776638419168 14 17.610829496059537 24.931960943646896 35.98072979143288 21.476479768860685 15 29.379973945233395 15.25364702240452 35.84271691883038 19.52366211353171 16 27.04084923061048 16.201679371589986 35.132660036889426 21.624811360910112 17 31.57399166763405 15.836654671155308 24.35669233448129 28.232661326729353 18 33.51584568355067 17.592771737027434 23.287415031794552 25.603967547627338 19 24.442624325248136 22.94640035599812 31.852802517783786 20.758172800969966 20 31.432311988494554 24.56967437780945 24.140799834899422 19.857213798796572 21 26.697495033924103 22.189974976137037 28.578154425612052 22.534375564326805 22 30.102027654524814 21.394850892580745 24.01970900835827 24.483412444536167 23 33.42598982283477 23.675775380031343 23.06179177442552 19.836443022708362 24 30.802022469011597 23.81495956351817 23.51184717976499 21.871170787705246 25 26.000761010467116 22.32017903093701 22.24343306010048 29.435626898495393 26 31.125832086278958 19.538546880643995 25.731461891738476 23.604159141338563 27 23.060799349891326 15.432986565710639 26.468710295322182 35.03750378907585 28 22.593837282056906 28.29212598933103 25.96868972901844 23.145346999593624 29 32.59802498758361 19.192192831388635 28.22036030108942 19.989421879938337 30 23.479467079583213 23.051711345527277 32.647504758218005 20.821316816671505 31 29.44053056695311 19.393693065985392 31.283063662873218 19.88271270418828 32 29.823463773631154 16.725469075522934 35.831629460974334 17.61943768987158 33 32.85069332473396 14.71396323766527 31.856175427281524 20.57916801031925 34 25.19027838695529 17.536572150984288 38.39753347609587 18.87561598596455 35 25.385563073668187 24.125831274631853 36.868279656589245 13.620325995110719 36 26.377007934971935 26.15831236694407 30.816076382168895 16.648603315915103 37 26.78576036747439 21.95749787102268 34.979097313617714 16.277644447885216 38 23.541596757956686 24.61152267623448 34.53124596680519 17.31563459900364 39 28.43280837693633 19.651348090608206 31.670290392717277 20.24555313973819 40 22.141857058979138 22.567054010981565 34.204804273906845 21.086284656132452 41 23.059751085532895 20.448665423599778 36.10098520578349 20.39059828508384 42 23.098369754624194 20.46906664257225 33.72013475662489 22.71242884617867 43 21.76918162281466 18.867656644272778 33.400450459171495 25.962711273741068 44 23.833979599078667 15.899427716090402 37.67783061170504 22.588762073125885 45 25.789365024064825 19.740254712964038 33.803661982735264 20.666718280235873 46 24.87192061013537 23.227858719077055 32.75799770295905 19.142222967828523 47 21.065847313757512 19.750321135561162 36.06981713024226 23.11401442043907 48 23.376061756125267 20.88980972297524 36.7658069346552 18.96832158624429 49 25.278414409761453 21.141418380193034 34.612479421543625 18.967687788501888 50 19.33682396007515 21.249782104835013 36.12798677779858 23.285407157291257 51 20.599398406940267 19.775758123442767 37.29941518957927 22.325428280037695 52 20.327727079111433 20.787242006892722 38.42564506344146 20.459385850554387 53 18.608524979662462 21.122632129437136 41.9857185284661 18.283124362434304 54 18.974968201803943 21.11335653364152 36.885261778245514 23.026413486309018 55 21.847816961892892 18.886298618689985 36.0742123174882 23.191672101928926 56 19.33967138576005 23.495143122868658 31.55097137542627 25.61421411594502 57 22.9868578255675 23.770221834737963 31.609674190319353 21.633246149375182 58 24.078977962642416 25.00452114604593 31.740382876482293 19.17611801482936 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 78.0 12 156.0 13 150.5 14 145.0 15 145.0 16 257.5 17 370.0 18 718.5 19 1067.0 20 1385.5 21 1704.0 22 1697.0 23 1727.0 24 1764.0 25 2631.5 26 3499.0 27 3474.5 28 3769.0 29 4088.0 30 4273.0 31 4458.0 32 4145.0 33 3832.0 34 3556.0 35 4072.0 36 4864.0 37 3555.0 38 2246.0 39 2295.5 40 2345.0 41 2334.5 42 2015.0 43 1706.0 44 1905.0 45 2104.0 46 2431.0 47 4025.5 48 5293.0 49 4360.0 50 3427.0 51 3729.5 52 4032.0 53 4159.0 54 6098.0 55 7910.0 56 7902.5 57 7895.0 58 7326.0 59 7659.0 60 8561.0 61 10483.5 62 12406.0 63 12423.5 64 12441.0 65 11318.5 66 9123.5 67 8051.0 68 6696.5 69 5342.0 70 4603.5 71 3865.0 72 3038.0 73 2004.5 74 1798.0 75 1485.0 76 1172.0 77 911.0 78 490.5 79 331.0 80 255.5 81 180.0 82 151.5 83 123.0 84 89.0 85 48.5 86 42.0 87 30.0 88 18.0 89 14.0 90 10.0 91 9.0 92 5.0 93 2.0 94 1.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 6.449199654322899E-4 20 6.449199654322899E-4 21 0.0038695197925937394 22 0.0012898399308645797 23 0.0032245998271614494 24 0.0 25 6.449199654322899E-4 26 0.016767919101239537 27 0.004514439758026029 28 0.018702678997536408 29 0.013543319274078086 30 0.04062995782223426 31 0.03482567813334365 32 0.012253479343213506 33 0.005159359723458319 34 0.014188239239510377 35 0.016122999135807246 36 0.030956158340749915 37 0.03482567813334365 38 0.06062247675063524 39 0.03934011789136968 40 0.04578931754569258 41 0.04191979775309884 42 0.07223103612841647 43 0.06771659637039043 44 0.04191979775309884 45 0.03869519792593739 46 0.04901391737285402 47 0.08964387519508829 48 0.08125991564446852 49 0.10512195436546326 50 0.1077016342271924 51 0.08770911529879143 52 0.07223103612841647 53 0.11092623405435387 54 0.11286099395065073 55 0.13220859291361942 56 0.1457519121876975 57 0.13736795263707774 58 0.14833159204942664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 58 155058.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.310219401772237 #Duplication Level Percentage of deduplicated Percentage of total 1 70.11884899791963 21.954365463246013 2 10.787039897835177 6.754891717937804 3 5.056746791900966 4.749835545408815 4 2.943417990071886 3.6863625224109686 5 2.032997590063647 3.1826800294083504 6 1.518053924900616 2.8518360871415855 7 1.1926095285175802 2.6138606198970704 8 0.9021813013656306 2.2597995588747435 9 0.7394591031741128 2.0837364083117285 >10 4.117489546643597 22.946897290046305 >50 0.37075943891738244 7.962181893227051 >100 0.19979814208325608 10.995885410620541 >500 0.012358647963912749 2.6680338969933834 >1k 0.008239098642608498 5.289633556475641 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 2497 1.6103651536844277 No Hit GAGGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC 2399 1.5471629970720633 No Hit GAGGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG 1668 1.0757265023410594 No Hit GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA 1638 1.0563789033780908 No Hit GAGGAGGGGGACACATTTTTAAAAAATATAAATTGTATGTTCATTATAAAGAAATTGT 987 0.6365360058816701 No Hit GAGGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 866 0.558500690064363 No Hit GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 636 0.41016909801493634 No Hit GAGGAGGGGACACCTATGTCAACACACCAACTGGGGATGAGGATGGCAGAAGTCGCCA 584 0.37663325981245727 No Hit GAGGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 547 0.35277122109146253 No Hit GAGGAGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 517 0.33342362212849386 No Hit GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA 447 0.28827922454823357 No Hit GAGGAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG 436 0.28118510492847837 No Hit GAGGAGGGGAACTATCTGTAAACCAACCTTTCAGTTGCTATATAACTTCCATAGACAT 378 0.24377974693340557 No Hit GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA 351 0.22636690786673372 No Hit GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA 300 0.19347598962968696 No Hit GAGGAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA 300 0.19347598962968696 No Hit GAGGAGGGGACGATACCTTTTTCTTGGTGATTATGTGGACAGAGGTTATTTTAGTATA 283 0.18251235021733803 No Hit GAGGAGGGGGCTCATTATATTCAAATATTCATTTTAGGAGCCATTCCGTAGTGCCATT 275 0.1773529904938797 No Hit GAGGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 270 0.17412839066671826 No Hit GAGGAGGGGGCATCCTTCCGGCTCCGTCGTGGAAGGAGGACTGCGCCTCGTCTTCCTG 269 0.17348347070128597 No Hit GAGGAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGATCTTCCAAA 267 0.1721936307704214 No Hit GAGGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC 266 0.1715487108049891 No Hit GAGGAGGGGGTATCAGGTCGACCCTGATGCCTGCTTTTCGGCCAAAGTGAACAACTCT 265 0.1709037908395568 No Hit GAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA 261 0.16832411097782765 No Hit GAGGAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA 255 0.16445459118523392 No Hit GAGGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA 248 0.15994015142720788 No Hit GAGGAGGGGGACTTCCTGTCAGCGAGCTGAATCCCCTGGAGATGGGGGGGTGGGCTGA 242 0.15607063163461413 No Hit GAGGAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT 239 0.15413587173831728 No Hit GAGGAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC 229 0.14768667208399439 No Hit GAGGAGGGGGGTCACAACTGAAATCAGCATCCTGATTACTTTGTGTTCCAGTGTTCTG 229 0.14768667208399439 No Hit GAGGAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG 228 0.14704175211856207 No Hit GAGGAGGGGAACTTCTTAAAAGACAAAAAAGAGAGAAATTGTATCCCCGTGCATGCTA 224 0.14446207225683294 No Hit GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGCTCTTCCGA 224 0.14446207225683294 No Hit GAGGAGGGGATTGCAGATGGTGGTTTTACTGAGTTGCACTCACTGTGGCAGAATGAAG 210 0.13543319274078086 No Hit GAGGAGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG 209 0.13478827277534858 No Hit GAGGAGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG 203 0.13091875298275485 No Hit GAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 198 0.1276941531555934 No Hit GAGGAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA 197 0.1270492331901611 No Hit GAGGAGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC 195 0.12575939325929653 No Hit GAGGAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA 194 0.12511447329386424 No Hit GAGGAGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC 193 0.12446955332843193 No Hit GAGGAGGGGCCACCTTCCATGAGCAGCTTGCCCAGGCACTCCAGGTCTGAGAAGCAAG 190 0.12253479343213507 No Hit GAGGAGGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA 185 0.11931019360497362 No Hit GAGGAGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC 180 0.11608559377781218 No Hit GAGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG 179 0.11544067381237988 No Hit GAGGAGGGGGAACTTGTCAAGACATGGTCGATCTTCCTCAAGCTAGAAGCATGGAGTC 178 0.11479575384694758 No Hit GAGGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTAAAAAACATCGAAC 175 0.11286099395065073 No Hit GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG 174 0.11221607398521843 No Hit GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGA 172 0.11092623405435387 No Hit GAGGAGGGGGAGCCCAAGGGTGAATGGCCTGACCACAGTAGCCTTGAAAGACACCAGC 172 0.11092623405435387 No Hit GAGGAGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACCAA 169 0.10899147415805699 No Hit GAGGAGGGGCAATTGGGTTGTGCCTTTGCGTTCGAATGCTGACCCATGCAATATGGCC 166 0.10705671426176012 No Hit GAGGAGGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGGGC 164 0.10576687433089553 No Hit GAGGAGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 163 0.10512195436546326 No Hit GAGGAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT 161 0.10383211443459867 No Hit GAGGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCCG 160 0.10318719446916638 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.011608559377781217 0.0 0.0 0.0 0.0 11 0.026441718582723883 0.0 0.0 0.0 0.0 12 0.026441718582723883 0.0 0.0 0.0 0.0 13 0.026441718582723883 0.0 0.0 0.0 0.0 14 0.03869519792593739 0.0 0.0 0.0 0.0 15 0.03934011789136968 0.0 0.0 0.0 0.0 16 0.04127487778766655 0.0 0.0 0.0 0.0 17 0.04191979775309884 0.0 0.0 0.0 0.0 18 0.04256471771853113 0.0 0.0 0.0 0.0 19 0.04256471771853113 0.0 0.0 0.0 0.0 20 0.04707915747655716 0.0 0.0 0.0 0.0 21 0.04901391737285403 0.0 0.0 0.0 0.0 22 0.04965883733828632 0.0 0.0 0.0 0.0 23 0.0573978769234738 0.0 0.0 0.0 0.0 24 0.06707167640495815 0.0 0.0 0.0 0.0 25 0.06836151633582273 0.0 0.0 0.0 0.0 26 0.06900643630125501 0.0 0.0 0.0 0.0 27 0.07029627623211959 0.0 0.0 0.0 0.0 28 0.12898399308645797 0.0 0.0 0.0 0.0 29 0.13027383301732254 0.0 0.0 0.0 0.0 30 0.14252731236053606 0.0 0.0 0.0 0.0 31 0.14381715229140063 0.0 0.0 0.0 0.0 32 0.14446207225683294 0.0 0.0 0.0 0.0 33 0.14446207225683294 0.0 0.0 0.0 0.0 34 0.14510699222226522 0.0 0.0 0.0 0.0 35 0.2102439087309265 0.0 0.0 0.0 0.0 36 0.21411342852352022 0.0 0.0 0.0 0.0 37 0.21540326845438482 0.0 0.0 0.0 0.0 38 0.21669310838524938 0.0 0.0 0.0 0.0 39 0.22056262817784314 0.0 0.0 0.0 0.0 40 0.2218524681087077 0.0 0.0 0.0 0.0 41 0.22378722800500458 0.0 0.0 0.0 0.0 42 0.22701182783216603 0.0 0.0 0.0 0.0 43 0.2295915076938952 0.0 0.0 0.0 0.0 44 0.23023642765932748 0.0 0.0 0.0 0.0 45 0.23281610752105664 0.0 0.0 0.0 0.0 46 0.2341059474519212 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAACTC 35 3.8438884E-8 52.01775 51 CCTTACT 15 0.0071986956 52.01775 39 CTCAAGC 30 7.931285E-7 52.01775 37 TACTTCC 15 0.0071986956 52.01775 46 ACTGATC 40 1.87174E-9 52.01775 45 CTATATA 70 0.0 52.01775 38 TAGAATC 15 0.0071986956 52.01775 34 TAGGTGC 20 3.4325678E-4 52.01775 47 AGAAGTC 35 3.8438884E-8 52.01775 48 ACCTGAT 15 0.0071986956 52.01775 40 TATATGG 20 3.4325678E-4 52.01775 51 TCCTCAA 30 7.931285E-7 52.01775 35 TAAAATG 20 3.4325678E-4 52.01775 38 CAATTCT 15 0.0071986956 52.01775 51 TAAGTAC 30 7.931285E-7 52.01775 52 ATATGCT 15 0.0071986956 52.01775 34 TAACGGC 15 0.0071986956 52.01775 37 TTATATG 20 3.4325678E-4 52.01775 50 GCTCTTA 15 0.0071986956 52.01775 49 GTGTGAC 30 7.931285E-7 52.01775 34 >>END_MODULE