FastQCFastQC Report
Tue 31 May 2016
SRR212536_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212536_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154141
Sequences flagged as poor quality0
Sequence length58
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT25611.6614658007927805No Hit
GAGGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC23781.5427433324034487No Hit
GAGGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG16721.0847211319506167No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA15350.995841469823084No Hit
GAGGAGGGGGACACATTTTTAAAAAATATAAATTGTATGTTCATTATAAAGAAATTGT9750.6325377414185712No Hit
GAGGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT8470.5494968892118255No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG5900.3827664281404688No Hit
GAGGAGGGGACACCTATGTCAACACACCAACTGGGGATGAGGATGGCAGAAGTCGCCA5820.3775763748775472No Hit
GAGGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.3289196255376571No Hit
GAGGAGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4650.30167184590731866No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA4510.2925892526972058No Hit
GAGGAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG4110.26663898638259775No Hit
GAGGAGGGGAACTATCTGTAAACCAACCTTTCAGTTGCTATATAACTTCCATAGACAT3850.24977131327810254No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA3210.20825088717472962No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA3010.1952757540174256No Hit
GAGGAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2970.1926807273859648No Hit
GAGGAGGGGACGATACCTTTTTCTTGGTGATTATGTGGACAGAGGTTATTTTAGTATA2920.18943694409663878No Hit
GAGGAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA2770.17970559422866078No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGATCTTCCAAA2530.16413543443989595No Hit
GAGGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA2530.16413543443989595No Hit
GAGGAGGGGGTATCAGGTCGACCCTGATGCCTGCTTTTCGGCCAAAGTGAACAACTCT2500.16218916446630033No Hit
GAGGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.16154040780843512No Hit
GAGGAGGGGGCTCATTATATTCAAATATTCATTTTAGGAGCCATTCCGTAGTGCCATT2490.16154040780843512No Hit
GAGGAGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG2410.15635035454551352No Hit
GAGGAGGGGGCATCCTTCCGGCTCCGTCGTGGAAGGAGGACTGCGCCTCGTCTTCCTG2400.1557015978876483No Hit
GAGGAGGGGGACTTCCTGTCAGCGAGCTGAATCCCCTGGAGATGGGGGGGTGGGCTGA2380.15440408457191793No Hit
GAGGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC2310.1498627879668615No Hit
GAGGAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT2290.14856527465113112No Hit
GAGGAGGGGATTGCAGATGGTGGTTTTACTGAGTTGCACTCACTGTGGCAGAATGAAG2280.1479165179932659No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGCTCTTCCGA2210.1433752213882095No Hit
GAGGAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2170.1407801947567487No Hit
GAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA2100.1362388981516923No Hit
GAGGAGGGGAACTTCTTAAAAGACAAAAAAGAGAGAAATTGTATCCCCGTGCATGCTA2080.13494138483596188No Hit
GAGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG2040.13234635820450108No Hit
GAGGAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG2030.13169760154663587No Hit
GAGGAGGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA1990.12910257491517507No Hit
GAGGAGGGGGGAATTCCTGGCACTGGCTGGGTTCGCTACCGTAGCGCAGGCCGCTCCG1970.12780506159944466No Hit
GAGGAGGGGGGTCACAACTGAAATCAGCATCCTGATTACTTTGTGTTCCAGTGTTCTG1970.12780506159944466No Hit
GAGGAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGA1880.12196625167865784No Hit
GAGGAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA1870.12131749502079264No Hit
GAGGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTAAAAAACATCGAAC1860.12066873836292745No Hit
GAGGAGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC1830.11872246838933184No Hit
GAGGAGGGGCCACCTTCCATGAGCAGCTTGCCCAGGCACTCCAGGTCTGAGAAGCAAG1810.11742495507360143No Hit
GAGGAGGGGGAACTTGTCAAGACATGGTCGATCTTCCTCAAGCTAGAAGCATGGAGTC1790.11612744175787104No Hit
GAGGAGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGG1790.11612744175787104No Hit
GAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.11482992844214063No Hit
GAGGAGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC1760.11418117178427543No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG1690.10963987517921903No Hit
GAGGAGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC1680.10899111852135383No Hit
GAGGAGGGGCAATTGGGTTGTGCCTTTGCGTTCGAATGCTGACCCATGCAATATGGCC1650.10704484854775823No Hit
GAGGAGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAACAACG1610.10444982191629743No Hit
GAGGAGGGGCGACAACCTCATCTGTGTGGCATATCGCCATCAGTTCGACGTGGTAAAC1580.10250355194270182No Hit
GAGGAGGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTC1550.10055728196910621No Hit
GAGGAGGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT1550.10055728196910621No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAT150.00716906952.07184627
CATCAAT150.00716906952.07184627
ACGCCGG251.6350325E-552.07184227
TCGGCAC203.4149204E-452.07184227
AACATTC203.4149204E-452.07184227
TAGACAT700.052.05492852
ATGGAGT353.8220605E-852.05492851
AACCAAC700.052.05492821
ATAGACA700.052.05492851
AACGCGA353.8220605E-852.05492823
ATGGTCG353.8220605E-852.05492824
TGGTCGA353.8220605E-852.05492825
ACGCGAT251.6381831E-552.05492424
ACAACTC251.6381831E-552.05492451
ACTATGG150.00717829352.05492421
AGCCGGA203.4204076E-452.05492451
ACACGGC150.00717829352.05492451
TTAATAA203.4204076E-452.05492423
GTCCTAT150.00717829352.05492421
ATATGTC203.4204076E-452.05492423
Produced by FastQC (version 0.11.5)