FastQCFastQC Report
Tue 31 May 2016
SRR212535_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR212535_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153204
Sequences flagged as poor quality0
Sequence length58
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT21831.4248975222579043No Hit
GAGGAGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAAC21621.4111903083470407No Hit
GAGGAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATG14580.9516722800971255No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA14060.9177306075559385No Hit
GAGGAGGGGGACACATTTTTAAAAAATATAAATTGTATGTTCATTATAAAGAAATTGT8470.5528576277381791No Hit
GAGGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT8110.5293595467481267No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG6130.4001201013028381No Hit
GAGGAGGGGACACCTATGTCAACACACCAACTGGGGATGAGGATGGCAGAAGTCGCCA5110.333542205164356No Hit
GAGGAGGGGCCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4870.31787681783765437No Hit
GAGGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4750.31004412417430355No Hit
GAGGAGGGGGGAATTCCTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCCGCTCCG4600.300253257095115No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAA4270.27871334952090027No Hit
GAGGAGGGGAACTATCTGTAAACCAACCTTTCAGTTGCTATATAACTTCCATAGACAT3670.2395498812041461No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCCCGA2930.19124827028014935No Hit
GAGGAGGGGACGATACCTTTTTCTTGGTGATTATGTGGACAGAGGTTATTTTAGTATA2740.1788465053131772No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGA2720.17754105636928538No Hit
GAGGAGGGGGTATCAGGTCGACCCTGATGCCTGCTTTTCGGCCAAAGTGAACAACTCT2580.16840291376204278No Hit
GAGGAGGGGGACTTCCTGTCAGCGAGCTGAATCCCCTGGAGATGGGGGGGTGGGCTGA2560.16709746481815096No Hit
GAGGAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCA2410.15730659773896244No Hit
GAGGAGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGA2310.1507793530195034No Hit
GAGGAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAA2190.14294665935615258No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGATCTTCCAAA2180.1422939348842067No Hit
GAGGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.1422939348842067No Hit
GAGGAGGGGGCTCATTATATTCAAATATTCATTTTAGGAGCCATTCCGTAGTGCCATT2160.14098848594031488No Hit
GAGGAGGGGATTGCAGATGGTGGTTTTACTGAGTTGCACTCACTGTGGCAGAATGAAG2140.13968303699642307No Hit
GAGGAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC2110.13772486358058536No Hit
GAGGAGGGGGCCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCGG2080.13576669016474766No Hit
GAGGAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCTTCCGAA2060.13446124122085584No Hit
GAGGAGGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTC2060.13446124122085584No Hit
GAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAA1990.12989216991723454No Hit
GAGGAGGGGGCTCTTCCTGTCTGTATCAGGACGGCGCGTGGTCCACGCCGAGCGACTG1990.12989216991723454No Hit
GAGGAGGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCT1980.12923944544528865No Hit
GAGGAGGGGGCATCCTTCCGGCTCCGTCGTGGAAGGAGGACTGCGCCTCGTCTTCCTG1950.12728127202945091No Hit
GAGGAGGGGCCACCTTCCATGAGCAGCTTGCCCAGGCACTCCAGGTCTGAGAAGCAAG1920.12532309861361324No Hit
GAGGAGGGGGGTCACAACTGAAATCAGCATCCTGATTACTTTGTGTTCCAGTGTTCTG1920.12532309861361324No Hit
GAGGAGGGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTACA1890.12336492519777552No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCGACTGCTCTTCCGA1840.120101302838046No Hit
GAGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG1830.1194485783661001No Hit
GAGGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGC1830.1194485783661001No Hit
GAGGAGGGGAACTTCTTAAAAGACAAAAAAGAGAGAAATTGTATCCCCGTGCATGCTA1810.11814312942220831No Hit
GAGGAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT1790.11683768047831648No Hit
GAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.10965771128691156No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTG1670.10900498681496566No Hit
GAGGAGGGGGGAGCCTTGATCAGAGTAACTGTCTTGGCTACATTTCTTCTTTTGCCCC1630.10639408892718207No Hit
GAGGAGGGGACGGCATGTGAGCTTGGAGTCGCCCCTTGACACCTGCTAGGTGCTCCTG1560.10182501762356073No Hit
GAGGAGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTTACGGC1560.10182501762356073No Hit
GAGGAGGGGAGTTCTCGCCGGAGCTTGGGGAAGGAAGTCTCTCTGCGGAGGTCTGAGG1550.10117229315161483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTT203.337734E-452.3131379
GGTACCT251.5908066E-552.3131378
GATGTTC203.337734E-452.3131379
GGATGTT251.5908066E-552.3131378
GGATGTC307.626368E-752.3131378
GCAACTG150.00703903352.3131379
GAACTAT307.626368E-752.3131379
GATTTGT150.00703903352.3131379
ACTTATG150.00703903352.31313710
GGAAATT150.00703903352.3131378
GATGCCA353.67545E-852.3131379
TACTCCA150.00703903352.31313710
AACTATC307.626368E-752.31313710
GGCGAAT150.00703903352.3131379
AATCCCG150.007168279552.07309338
GATAATG150.007168279552.07309338
CTATATA251.6347374E-552.07309338
TACGGCT150.007168279552.07309339
AAAGTAG150.007168279552.07309339
TCCAAAT150.007168279552.07309338
Produced by FastQC (version 0.11.5)